Amino acid dipepetide frequency for Mycobacterium phage DS6A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
25.991AlaAla: 25.991 ± 1.819
1.208AlaCys: 1.208 ± 0.291
9.084AlaAsp: 9.084 ± 0.634
11.867AlaGlu: 11.867 ± 1.159
2.468AlaPhe: 2.468 ± 0.454
16.697AlaGly: 16.697 ± 1.425
1.523AlaHis: 1.523 ± 0.306
4.253AlaIle: 4.253 ± 0.458
4.253AlaLys: 4.253 ± 0.669
11.709AlaLeu: 11.709 ± 0.838
3.57AlaMet: 3.57 ± 0.416
2.678AlaAsn: 2.678 ± 0.364
7.088AlaPro: 7.088 ± 0.624
4.411AlaGln: 4.411 ± 0.664
9.661AlaArg: 9.661 ± 0.89
5.198AlaSer: 5.198 ± 0.583
7.088AlaThr: 7.088 ± 0.76
12.444AlaVal: 12.444 ± 1.374
2.993AlaTrp: 2.993 ± 0.356
2.31AlaTyr: 2.31 ± 0.388
0.0AlaXaa: 0.0 ± 0.0
Cys
0.735CysAla: 0.735 ± 0.203
0.263CysCys: 0.263 ± 0.144
0.473CysAsp: 0.473 ± 0.211
0.735CysGlu: 0.735 ± 0.242
0.158CysPhe: 0.158 ± 0.101
1.208CysGly: 1.208 ± 0.282
0.21CysHis: 0.21 ± 0.113
0.315CysIle: 0.315 ± 0.163
0.263CysLys: 0.263 ± 0.105
0.473CysLeu: 0.473 ± 0.142
0.158CysMet: 0.158 ± 0.095
0.158CysAsn: 0.158 ± 0.091
0.893CysPro: 0.893 ± 0.226
0.315CysGln: 0.315 ± 0.109
1.05CysArg: 1.05 ± 0.248
0.525CysSer: 0.525 ± 0.171
0.473CysThr: 0.473 ± 0.15
0.42CysVal: 0.42 ± 0.151
0.263CysTrp: 0.263 ± 0.114
0.053CysTyr: 0.053 ± 0.057
0.0CysXaa: 0.0 ± 0.0
Asp
8.979AspAla: 8.979 ± 0.683
0.63AspCys: 0.63 ± 0.212
4.988AspAsp: 4.988 ± 0.651
3.886AspGlu: 3.886 ± 0.475
1.365AspPhe: 1.365 ± 0.237
7.404AspGly: 7.404 ± 0.626
0.84AspHis: 0.84 ± 0.192
1.418AspIle: 1.418 ± 0.312
1.838AspLys: 1.838 ± 0.266
5.933AspLeu: 5.933 ± 0.53
1.208AspMet: 1.208 ± 0.226
1.313AspAsn: 1.313 ± 0.331
3.938AspPro: 3.938 ± 0.456
1.995AspGln: 1.995 ± 0.297
6.983AspArg: 6.983 ± 0.684
2.205AspSer: 2.205 ± 0.392
2.94AspThr: 2.94 ± 0.4
4.778AspVal: 4.778 ± 0.519
1.523AspTrp: 1.523 ± 0.259
1.68AspTyr: 1.68 ± 0.296
0.0AspXaa: 0.0 ± 0.0
Glu
9.556GluAla: 9.556 ± 0.778
0.42GluCys: 0.42 ± 0.143
3.098GluAsp: 3.098 ± 0.383
1.103GluGlu: 1.103 ± 0.192
1.785GluPhe: 1.785 ± 0.378
4.148GluGly: 4.148 ± 0.511
1.89GluHis: 1.89 ± 0.339
2.048GluIle: 2.048 ± 0.364
1.47GluLys: 1.47 ± 0.324
7.351GluLeu: 7.351 ± 0.568
0.735GluMet: 0.735 ± 0.198
0.788GluAsn: 0.788 ± 0.225
2.94GluPro: 2.94 ± 0.411
1.785GluGln: 1.785 ± 0.277
4.988GluArg: 4.988 ± 0.65
1.733GluSer: 1.733 ± 0.331
2.835GluThr: 2.835 ± 0.405
3.623GluVal: 3.623 ± 0.434
1.575GluTrp: 1.575 ± 0.245
1.418GluTyr: 1.418 ± 0.229
0.0GluXaa: 0.0 ± 0.0
Phe
3.255PheAla: 3.255 ± 0.372
0.158PheCys: 0.158 ± 0.087
2.31PheAsp: 2.31 ± 0.358
1.365PheGlu: 1.365 ± 0.266
0.42PhePhe: 0.42 ± 0.171
2.888PheGly: 2.888 ± 0.573
0.683PheHis: 0.683 ± 0.193
1.313PheIle: 1.313 ± 0.233
0.578PheLys: 0.578 ± 0.203
1.103PheLeu: 1.103 ± 0.187
0.315PheMet: 0.315 ± 0.109
0.788PheAsn: 0.788 ± 0.216
1.47PhePro: 1.47 ± 0.323
0.893PheGln: 0.893 ± 0.245
1.155PheArg: 1.155 ± 0.21
0.788PheSer: 0.788 ± 0.204
1.838PheThr: 1.838 ± 0.29
1.208PheVal: 1.208 ± 0.247
0.315PheTrp: 0.315 ± 0.167
0.683PheTyr: 0.683 ± 0.219
0.0PheXaa: 0.0 ± 0.0
Gly
11.657GlyAla: 11.657 ± 1.522
0.683GlyCys: 0.683 ± 0.164
6.406GlyAsp: 6.406 ± 0.588
6.301GlyGlu: 6.301 ± 0.704
2.363GlyPhe: 2.363 ± 0.339
11.919GlyGly: 11.919 ± 1.382
1.785GlyHis: 1.785 ± 0.286
3.36GlyIle: 3.36 ± 0.581
3.308GlyLys: 3.308 ± 0.628
6.983GlyLeu: 6.983 ± 0.653
2.258GlyMet: 2.258 ± 0.302
2.993GlyAsn: 2.993 ± 0.527
4.936GlyPro: 4.936 ± 0.593
3.676GlyGln: 3.676 ± 0.481
8.244GlyArg: 8.244 ± 0.744
4.358GlySer: 4.358 ± 0.444
6.091GlyThr: 6.091 ± 0.537
7.614GlyVal: 7.614 ± 0.697
2.52GlyTrp: 2.52 ± 0.4
2.31GlyTyr: 2.31 ± 0.339
0.0GlyXaa: 0.0 ± 0.0
His
2.258HisAla: 2.258 ± 0.391
0.473HisCys: 0.473 ± 0.155
1.26HisAsp: 1.26 ± 0.261
0.84HisGlu: 0.84 ± 0.199
0.473HisPhe: 0.473 ± 0.131
2.258HisGly: 2.258 ± 0.387
0.578HisHis: 0.578 ± 0.178
1.523HisIle: 1.523 ± 0.338
0.683HisLys: 0.683 ± 0.159
1.628HisLeu: 1.628 ± 0.399
0.315HisMet: 0.315 ± 0.116
0.525HisAsn: 0.525 ± 0.147
0.945HisPro: 0.945 ± 0.252
0.788HisGln: 0.788 ± 0.209
2.153HisArg: 2.153 ± 0.389
0.998HisSer: 0.998 ± 0.209
1.733HisThr: 1.733 ± 0.324
1.365HisVal: 1.365 ± 0.248
0.578HisTrp: 0.578 ± 0.183
0.368HisTyr: 0.368 ± 0.133
0.0HisXaa: 0.0 ± 0.0
Ile
6.353IleAla: 6.353 ± 0.615
0.315IleCys: 0.315 ± 0.134
3.413IleAsp: 3.413 ± 0.395
3.098IleGlu: 3.098 ± 0.416
0.525IlePhe: 0.525 ± 0.161
3.203IleGly: 3.203 ± 0.453
0.945IleHis: 0.945 ± 0.212
0.788IleIle: 0.788 ± 0.164
1.05IleLys: 1.05 ± 0.251
2.258IleLeu: 2.258 ± 0.286
0.525IleMet: 0.525 ± 0.135
0.578IleAsn: 0.578 ± 0.178
1.838IlePro: 1.838 ± 0.381
0.84IleGln: 0.84 ± 0.229
2.1IleArg: 2.1 ± 0.375
1.208IleSer: 1.208 ± 0.204
2.573IleThr: 2.573 ± 0.379
2.993IleVal: 2.993 ± 0.347
0.578IleTrp: 0.578 ± 0.153
0.735IleTyr: 0.735 ± 0.153
0.0IleXaa: 0.0 ± 0.0
Lys
5.093LysAla: 5.093 ± 0.673
0.263LysCys: 0.263 ± 0.115
1.313LysAsp: 1.313 ± 0.246
0.893LysGlu: 0.893 ± 0.178
0.893LysPhe: 0.893 ± 0.202
2.835LysGly: 2.835 ± 0.457
0.63LysHis: 0.63 ± 0.183
1.103LysIle: 1.103 ± 0.353
0.683LysLys: 0.683 ± 0.194
2.678LysLeu: 2.678 ± 0.328
0.683LysMet: 0.683 ± 0.173
0.578LysAsn: 0.578 ± 0.181
1.943LysPro: 1.943 ± 0.346
1.155LysGln: 1.155 ± 0.2
3.623LysArg: 3.623 ± 0.476
1.26LysSer: 1.26 ± 0.238
1.365LysThr: 1.365 ± 0.272
1.68LysVal: 1.68 ± 0.274
0.84LysTrp: 0.84 ± 0.175
0.473LysTyr: 0.473 ± 0.163
0.0LysXaa: 0.0 ± 0.0
Leu
12.287LeuAla: 12.287 ± 0.865
0.735LeuCys: 0.735 ± 0.193
6.616LeuAsp: 6.616 ± 0.564
3.781LeuGlu: 3.781 ± 0.478
1.995LeuPhe: 1.995 ± 0.394
8.139LeuGly: 8.139 ± 0.741
2.258LeuHis: 2.258 ± 0.337
3.098LeuIle: 3.098 ± 0.395
2.1LeuLys: 2.1 ± 0.361
6.353LeuLeu: 6.353 ± 0.86
1.68LeuMet: 1.68 ± 0.274
1.628LeuAsn: 1.628 ± 0.321
4.568LeuPro: 4.568 ± 0.539
1.785LeuGln: 1.785 ± 0.243
6.511LeuArg: 6.511 ± 0.756
3.676LeuSer: 3.676 ± 0.422
5.251LeuThr: 5.251 ± 0.486
4.988LeuVal: 4.988 ± 0.507
1.575LeuTrp: 1.575 ± 0.269
1.523LeuTyr: 1.523 ± 0.289
0.0LeuXaa: 0.0 ± 0.0
Met
2.888MetAla: 2.888 ± 0.329
0.105MetCys: 0.105 ± 0.068
0.945MetAsp: 0.945 ± 0.196
0.473MetGlu: 0.473 ± 0.16
0.63MetPhe: 0.63 ± 0.144
1.995MetGly: 1.995 ± 0.402
0.315MetHis: 0.315 ± 0.123
0.893MetIle: 0.893 ± 0.2
0.578MetLys: 0.578 ± 0.165
1.365MetLeu: 1.365 ± 0.238
0.525MetMet: 0.525 ± 0.141
0.63MetAsn: 0.63 ± 0.15
1.68MetPro: 1.68 ± 0.275
0.735MetGln: 0.735 ± 0.211
1.313MetArg: 1.313 ± 0.275
1.05MetSer: 1.05 ± 0.199
2.52MetThr: 2.52 ± 0.422
1.155MetVal: 1.155 ± 0.239
0.578MetTrp: 0.578 ± 0.182
0.473MetTyr: 0.473 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
2.835AsnAla: 2.835 ± 0.416
0.105AsnCys: 0.105 ± 0.069
1.785AsnAsp: 1.785 ± 0.314
0.998AsnGlu: 0.998 ± 0.227
0.368AsnPhe: 0.368 ± 0.125
2.94AsnGly: 2.94 ± 0.576
0.525AsnHis: 0.525 ± 0.188
0.84AsnIle: 0.84 ± 0.184
0.945AsnLys: 0.945 ± 0.216
1.733AsnLeu: 1.733 ± 0.338
0.525AsnMet: 0.525 ± 0.19
0.473AsnAsn: 0.473 ± 0.189
1.313AsnPro: 1.313 ± 0.193
0.473AsnGln: 0.473 ± 0.142
1.733AsnArg: 1.733 ± 0.321
0.788AsnSer: 0.788 ± 0.219
1.523AsnThr: 1.523 ± 0.236
1.523AsnVal: 1.523 ± 0.227
0.735AsnTrp: 0.735 ± 0.194
0.84AsnTyr: 0.84 ± 0.18
0.0AsnXaa: 0.0 ± 0.0
Pro
10.291ProAla: 10.291 ± 0.778
0.578ProCys: 0.578 ± 0.191
3.465ProAsp: 3.465 ± 0.419
2.52ProGlu: 2.52 ± 0.398
1.365ProPhe: 1.365 ± 0.256
6.668ProGly: 6.668 ± 0.524
1.208ProHis: 1.208 ± 0.238
2.205ProIle: 2.205 ± 0.324
1.628ProLys: 1.628 ± 0.336
3.15ProLeu: 3.15 ± 0.341
0.945ProMet: 0.945 ± 0.216
1.68ProAsn: 1.68 ± 0.324
3.886ProPro: 3.886 ± 0.573
0.945ProGln: 0.945 ± 0.208
3.465ProArg: 3.465 ± 0.396
1.943ProSer: 1.943 ± 0.316
3.36ProThr: 3.36 ± 0.435
4.411ProVal: 4.411 ± 0.512
1.26ProTrp: 1.26 ± 0.304
1.155ProTyr: 1.155 ± 0.193
0.0ProXaa: 0.0 ± 0.0
Gln
4.673GlnAla: 4.673 ± 0.538
0.42GlnCys: 0.42 ± 0.143
1.418GlnAsp: 1.418 ± 0.287
1.05GlnGlu: 1.05 ± 0.24
0.683GlnPhe: 0.683 ± 0.197
1.785GlnGly: 1.785 ± 0.413
1.05GlnHis: 1.05 ± 0.244
1.418GlnIle: 1.418 ± 0.261
1.05GlnLys: 1.05 ± 0.216
3.518GlnLeu: 3.518 ± 0.413
0.998GlnMet: 0.998 ± 0.262
0.63GlnAsn: 0.63 ± 0.197
1.89GlnPro: 1.89 ± 0.316
1.418GlnGln: 1.418 ± 0.267
3.413GlnArg: 3.413 ± 0.486
1.208GlnSer: 1.208 ± 0.261
1.26GlnThr: 1.26 ± 0.264
1.89GlnVal: 1.89 ± 0.249
0.84GlnTrp: 0.84 ± 0.231
0.683GlnTyr: 0.683 ± 0.186
0.0GlnXaa: 0.0 ± 0.0
Arg
9.976ArgAla: 9.976 ± 1.0
0.683ArgCys: 0.683 ± 0.218
5.408ArgAsp: 5.408 ± 0.736
4.358ArgGlu: 4.358 ± 0.448
2.468ArgPhe: 2.468 ± 0.356
5.828ArgGly: 5.828 ± 0.585
2.678ArgHis: 2.678 ± 0.4
3.36ArgIle: 3.36 ± 0.411
2.73ArgLys: 2.73 ± 0.443
5.461ArgLeu: 5.461 ± 0.518
2.258ArgMet: 2.258 ± 0.347
1.943ArgAsn: 1.943 ± 0.281
5.356ArgPro: 5.356 ± 0.569
3.045ArgGln: 3.045 ± 0.458
9.661ArgArg: 9.661 ± 1.298
3.045ArgSer: 3.045 ± 0.414
4.096ArgThr: 4.096 ± 0.505
5.198ArgVal: 5.198 ± 0.64
2.73ArgTrp: 2.73 ± 0.365
2.468ArgTyr: 2.468 ± 0.284
0.0ArgXaa: 0.0 ± 0.0
Ser
5.513SerAla: 5.513 ± 0.684
0.315SerCys: 0.315 ± 0.136
2.73SerAsp: 2.73 ± 0.486
1.575SerGlu: 1.575 ± 0.226
1.103SerPhe: 1.103 ± 0.213
4.306SerGly: 4.306 ± 0.619
0.84SerHis: 0.84 ± 0.215
1.575SerIle: 1.575 ± 0.273
1.313SerLys: 1.313 ± 0.291
2.94SerLeu: 2.94 ± 0.386
0.945SerMet: 0.945 ± 0.215
1.208SerAsn: 1.208 ± 0.332
1.575SerPro: 1.575 ± 0.261
0.84SerGln: 0.84 ± 0.22
3.308SerArg: 3.308 ± 0.344
1.47SerSer: 1.47 ± 0.316
2.363SerThr: 2.363 ± 0.342
3.465SerVal: 3.465 ± 0.593
1.26SerTrp: 1.26 ± 0.298
0.998SerTyr: 0.998 ± 0.233
0.0SerXaa: 0.0 ± 0.0
Thr
6.773ThrAla: 6.773 ± 0.577
0.578ThrCys: 0.578 ± 0.189
3.781ThrAsp: 3.781 ± 0.45
4.043ThrGlu: 4.043 ± 0.41
1.208ThrPhe: 1.208 ± 0.266
5.881ThrGly: 5.881 ± 0.579
0.998ThrHis: 0.998 ± 0.262
2.678ThrIle: 2.678 ± 0.411
1.68ThrLys: 1.68 ± 0.313
4.988ThrLeu: 4.988 ± 0.457
0.84ThrMet: 0.84 ± 0.221
1.26ThrAsn: 1.26 ± 0.321
3.938ThrPro: 3.938 ± 0.521
1.733ThrGln: 1.733 ± 0.255
3.886ThrArg: 3.886 ± 0.492
2.153ThrSer: 2.153 ± 0.445
4.201ThrThr: 4.201 ± 0.465
5.461ThrVal: 5.461 ± 0.587
1.313ThrTrp: 1.313 ± 0.281
1.628ThrTyr: 1.628 ± 0.325
0.0ThrXaa: 0.0 ± 0.0
Val
11.499ValAla: 11.499 ± 0.99
0.525ValCys: 0.525 ± 0.153
4.516ValAsp: 4.516 ± 0.435
3.728ValGlu: 3.728 ± 0.402
1.943ValPhe: 1.943 ± 0.343
6.301ValGly: 6.301 ± 0.68
1.68ValHis: 1.68 ± 0.313
2.363ValIle: 2.363 ± 0.347
2.625ValLys: 2.625 ± 0.36
6.406ValLeu: 6.406 ± 0.622
1.418ValMet: 1.418 ± 0.337
1.785ValAsn: 1.785 ± 0.237
3.938ValPro: 3.938 ± 0.442
2.415ValGln: 2.415 ± 0.325
4.621ValArg: 4.621 ± 0.602
4.358ValSer: 4.358 ± 0.61
4.621ValThr: 4.621 ± 0.569
6.406ValVal: 6.406 ± 0.609
1.155ValTrp: 1.155 ± 0.212
1.208ValTyr: 1.208 ± 0.285
0.0ValXaa: 0.0 ± 0.0
Trp
3.465TrpAla: 3.465 ± 0.353
0.368TrpCys: 0.368 ± 0.135
1.523TrpAsp: 1.523 ± 0.225
1.313TrpGlu: 1.313 ± 0.234
0.683TrpPhe: 0.683 ± 0.17
1.575TrpGly: 1.575 ± 0.307
0.683TrpHis: 0.683 ± 0.199
0.788TrpIle: 0.788 ± 0.179
0.315TrpLys: 0.315 ± 0.125
2.835TrpLeu: 2.835 ± 0.381
0.42TrpMet: 0.42 ± 0.131
0.63TrpAsn: 0.63 ± 0.186
0.84TrpPro: 0.84 ± 0.244
1.208TrpGln: 1.208 ± 0.309
2.468TrpArg: 2.468 ± 0.38
0.735TrpSer: 0.735 ± 0.231
1.208TrpThr: 1.208 ± 0.227
1.523TrpVal: 1.523 ± 0.266
0.42TrpTrp: 0.42 ± 0.139
0.525TrpTyr: 0.525 ± 0.153
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.468TyrAla: 2.468 ± 0.386
0.315TyrCys: 0.315 ± 0.12
1.313TyrAsp: 1.313 ± 0.3
1.365TyrGlu: 1.365 ± 0.252
0.63TyrPhe: 0.63 ± 0.225
1.785TyrGly: 1.785 ± 0.288
0.42TyrHis: 0.42 ± 0.115
0.578TyrIle: 0.578 ± 0.171
0.893TyrLys: 0.893 ± 0.204
1.68TyrLeu: 1.68 ± 0.308
0.473TyrMet: 0.473 ± 0.154
0.735TyrAsn: 0.735 ± 0.212
1.155TyrPro: 1.155 ± 0.274
0.84TyrGln: 0.84 ± 0.204
2.415TyrArg: 2.415 ± 0.373
0.945TyrSer: 0.945 ± 0.226
1.68TyrThr: 1.68 ± 0.285
1.365TyrVal: 1.365 ± 0.236
0.473TyrTrp: 0.473 ± 0.183
0.525TyrTyr: 0.525 ± 0.248
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (19046 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski