Thermomonas hydrothermalis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Thermomonas

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2296 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M4YQN7|A0A1M4YQN7_9GAMM Dihydropteroate synthase OS=Thermomonas hydrothermalis OX=213588 GN=SAMN02745204_01744 PE=3 SV=1
MM1 pKa = 7.87SDD3 pKa = 3.11EE4 pKa = 4.5TLNGAAPSTDD14 pKa = 3.39APQGPLFTVEE24 pKa = 3.88KK25 pKa = 10.3LYY27 pKa = 11.46VKK29 pKa = 10.29DD30 pKa = 4.92LSFEE34 pKa = 4.29APNAPQVFNEE44 pKa = 3.98QGQPDD49 pKa = 4.54LQMKK53 pKa = 9.88LNQKK57 pKa = 7.8VEE59 pKa = 4.17RR60 pKa = 11.84LAEE63 pKa = 3.85NAYY66 pKa = 9.48EE67 pKa = 4.06VSLGITLTCTLNGKK81 pKa = 6.4TAYY84 pKa = 10.03LAEE87 pKa = 4.21VEE89 pKa = 4.29QCGVFGLAGMEE100 pKa = 3.91APMLDD105 pKa = 3.32AMLGIQCPTILYY117 pKa = 8.35PYY119 pKa = 9.71ASAAITQLITSGGFPPFPMQPINFEE144 pKa = 4.03ALYY147 pKa = 11.07AEE149 pKa = 5.07ALRR152 pKa = 11.84QRR154 pKa = 11.84AAQIGQQDD162 pKa = 3.67GALAADD168 pKa = 4.02TDD170 pKa = 4.25TAGNAA175 pKa = 3.47

Molecular weight: 18.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4U0M7|A0A1M4U0M7_9GAMM GMP synthase (Glutamine-hydrolysing) OS=Thermomonas hydrothermalis OX=213588 GN=SAMN02745204_00578 PE=4 SV=1
MM1 pKa = 7.49TSPEE5 pKa = 3.98GVPAGFWQRR14 pKa = 11.84YY15 pKa = 6.38AAWSLDD21 pKa = 3.7AVCLLPLVAGLAWSPLQRR39 pKa = 11.84AWAQAAQALKK49 pKa = 9.72TLTALLGRR57 pKa = 11.84QLEE60 pKa = 4.19IALASGQPPQALLSGVLADD79 pKa = 4.23PQVQAGAAVLNAALFTLFGLPVLLYY104 pKa = 10.84ALLSALWTLGFEE116 pKa = 4.52GSAWQATPGKK126 pKa = 9.49RR127 pKa = 11.84VLGLAVTDD135 pKa = 3.95SQGGKK140 pKa = 9.47PSLARR145 pKa = 11.84LALRR149 pKa = 11.84WLAAGLSWASLNLGHH164 pKa = 7.45ALAAVPPHH172 pKa = 6.49LALHH176 pKa = 6.69DD177 pKa = 4.33RR178 pKa = 11.84LSGTCVQTHH187 pKa = 6.05SGTRR191 pKa = 11.84RR192 pKa = 11.84LPRR195 pKa = 11.84WAQAWLGLQSVGALLALIWLWRR217 pKa = 11.84LVQAWTLAALGMAAPGG233 pKa = 3.36

Molecular weight: 24.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2296

0

2296

745712

39

2442

324.8

35.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.264 ± 0.08

0.891 ± 0.017

5.645 ± 0.039

5.148 ± 0.06

3.199 ± 0.029

8.206 ± 0.06

2.404 ± 0.027

4.175 ± 0.038

2.695 ± 0.048

11.532 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.113 ± 0.024

2.283 ± 0.035

5.743 ± 0.047

4.088 ± 0.039

7.989 ± 0.047

4.493 ± 0.038

4.973 ± 0.034

7.337 ± 0.04

1.573 ± 0.027

2.25 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski