Amino acid dipepetide frequency for Saphire II virus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.225AlaAla: 3.225 ± 2.59
1.188AlaCys: 1.188 ± 0.437
2.206AlaAsp: 2.206 ± 0.523
3.055AlaGlu: 3.055 ± 1.202
2.206AlaPhe: 2.206 ± 0.834
1.697AlaGly: 1.697 ± 1.118
0.849AlaHis: 0.849 ± 0.265
2.716AlaIle: 2.716 ± 0.769
3.055AlaLys: 3.055 ± 0.339
5.771AlaLeu: 5.771 ± 2.471
0.509AlaMet: 0.509 ± 0.459
2.546AlaAsn: 2.546 ± 1.185
1.188AlaPro: 1.188 ± 0.282
0.679AlaGln: 0.679 ± 0.388
3.055AlaArg: 3.055 ± 0.99
4.243AlaSer: 4.243 ± 0.56
3.225AlaThr: 3.225 ± 0.33
4.413AlaVal: 4.413 ± 1.121
0.509AlaTrp: 0.509 ± 0.44
2.376AlaTyr: 2.376 ± 0.984
0.0AlaXaa: 0.0 ± 0.0
Cys
1.867CysAla: 1.867 ± 1.745
1.358CysCys: 1.358 ± 0.529
0.679CysAsp: 0.679 ± 0.192
1.358CysGlu: 1.358 ± 0.434
2.376CysPhe: 2.376 ± 0.172
1.697CysGly: 1.697 ± 0.485
0.849CysHis: 0.849 ± 0.434
1.527CysIle: 1.527 ± 0.621
1.697CysLys: 1.697 ± 0.792
2.037CysLeu: 2.037 ± 0.735
0.339CysMet: 0.339 ± 0.158
1.018CysAsn: 1.018 ± 0.732
1.697CysPro: 1.697 ± 1.36
0.339CysGln: 0.339 ± 0.191
1.697CysArg: 1.697 ± 0.473
1.358CysSer: 1.358 ± 0.384
1.697CysThr: 1.697 ± 1.589
1.697CysVal: 1.697 ± 0.792
0.679CysTrp: 0.679 ± 0.317
1.018CysTyr: 1.018 ± 0.587
0.0CysXaa: 0.0 ± 0.0
Asp
1.527AspAla: 1.527 ± 0.631
1.188AspCys: 1.188 ± 0.329
2.716AspAsp: 2.716 ± 1.046
4.413AspGlu: 4.413 ± 0.864
2.037AspPhe: 2.037 ± 0.592
2.037AspGly: 2.037 ± 0.592
1.358AspHis: 1.358 ± 0.384
3.055AspIle: 3.055 ± 1.47
3.225AspLys: 3.225 ± 0.456
4.752AspLeu: 4.752 ± 0.188
1.697AspMet: 1.697 ± 1.815
3.225AspAsn: 3.225 ± 0.456
1.527AspPro: 1.527 ± 0.144
1.018AspGln: 1.018 ± 0.85
2.546AspArg: 2.546 ± 1.564
4.752AspSer: 4.752 ± 0.906
3.225AspThr: 3.225 ± 1.047
3.564AspVal: 3.564 ± 1.226
0.679AspTrp: 0.679 ± 0.388
1.697AspTyr: 1.697 ± 0.66
0.0AspXaa: 0.0 ± 0.0
Glu
2.885GluAla: 2.885 ± 1.46
2.376GluCys: 2.376 ± 1.029
3.564GluAsp: 3.564 ± 0.447
6.11GluGlu: 6.11 ± 0.899
3.055GluPhe: 3.055 ± 0.37
3.225GluGly: 3.225 ± 0.493
1.867GluHis: 1.867 ± 0.11
4.582GluIle: 4.582 ± 1.106
5.261GluLys: 5.261 ± 1.031
6.449GluLeu: 6.449 ± 1.294
1.527GluMet: 1.527 ± 0.631
2.716GluAsn: 2.716 ± 1.003
1.018GluPro: 1.018 ± 0.359
3.394GluGln: 3.394 ± 1.119
3.055GluArg: 3.055 ± 0.906
4.413GluSer: 4.413 ± 1.625
4.413GluThr: 4.413 ± 1.279
5.601GluVal: 5.601 ± 1.698
1.018GluTrp: 1.018 ± 0.85
2.376GluTyr: 2.376 ± 0.89
0.0GluXaa: 0.0 ± 0.0
Phe
1.358PheAla: 1.358 ± 0.777
1.188PheCys: 1.188 ± 0.492
2.376PheAsp: 2.376 ± 0.172
3.055PheGlu: 3.055 ± 0.558
2.376PhePhe: 2.376 ± 0.172
2.376PheGly: 2.376 ± 1.029
0.849PheHis: 0.849 ± 0.795
2.037PheIle: 2.037 ± 1.146
2.716PheLys: 2.716 ± 1.509
3.734PheLeu: 3.734 ± 0.522
0.509PheMet: 0.509 ± 0.148
2.206PheAsn: 2.206 ± 1.738
1.867PhePro: 1.867 ± 0.655
1.188PheGln: 1.188 ± 0.845
1.188PheArg: 1.188 ± 0.273
3.734PheSer: 3.734 ± 0.805
2.885PheThr: 2.885 ± 0.301
2.376PheVal: 2.376 ± 0.453
0.339PheTrp: 0.339 ± 0.158
2.037PheTyr: 2.037 ± 0.698
0.0PheXaa: 0.0 ± 0.0
Gly
2.376GlyAla: 2.376 ± 1.654
1.188GlyCys: 1.188 ± 1.046
2.376GlyAsp: 2.376 ± 0.472
2.206GlyGlu: 2.206 ± 0.631
1.188GlyPhe: 1.188 ± 0.946
1.188GlyGly: 1.188 ± 0.445
1.188GlyHis: 1.188 ± 0.445
2.546GlyIle: 2.546 ± 0.153
5.261GlyLys: 5.261 ± 1.198
4.413GlyLeu: 4.413 ± 0.733
1.867GlyMet: 1.867 ± 0.11
1.527GlyAsn: 1.527 ± 0.731
1.527GlyPro: 1.527 ± 0.601
1.527GlyGln: 1.527 ± 0.631
2.885GlyArg: 2.885 ± 1.094
5.431GlySer: 5.431 ± 0.839
2.546GlyThr: 2.546 ± 1.535
2.546GlyVal: 2.546 ± 0.74
0.509GlyTrp: 0.509 ± 0.633
1.527GlyTyr: 1.527 ± 0.301
0.0GlyXaa: 0.0 ± 0.0
His
1.188HisAla: 1.188 ± 0.445
1.358HisCys: 1.358 ± 0.887
1.188HisAsp: 1.188 ± 0.282
1.358HisGlu: 1.358 ± 0.434
0.849HisPhe: 0.849 ± 0.291
1.527HisGly: 1.527 ± 0.835
1.527HisHis: 1.527 ± 0.601
1.527HisIle: 1.527 ± 0.444
1.697HisLys: 1.697 ± 0.955
2.037HisLeu: 2.037 ± 0.277
1.358HisMet: 1.358 ± 1.149
1.527HisAsn: 1.527 ± 0.601
1.188HisPro: 1.188 ± 0.282
1.018HisGln: 1.018 ± 0.359
1.188HisArg: 1.188 ± 0.763
1.867HisSer: 1.867 ± 0.579
1.188HisThr: 1.188 ± 0.282
1.018HisVal: 1.018 ± 0.475
0.339HisTrp: 0.339 ± 0.158
0.679HisTyr: 0.679 ± 0.192
0.0HisXaa: 0.0 ± 0.0
Ile
3.055IleAla: 3.055 ± 0.339
1.867IleCys: 1.867 ± 0.396
2.885IleAsp: 2.885 ± 0.835
3.734IleGlu: 3.734 ± 1.403
2.037IlePhe: 2.037 ± 0.698
1.867IleGly: 1.867 ± 0.758
1.867IleHis: 1.867 ± 0.655
2.716IleIle: 2.716 ± 0.367
5.261IleLys: 5.261 ± 1.608
6.11IleLeu: 6.11 ± 1.125
1.018IleMet: 1.018 ± 0.296
1.697IleAsn: 1.697 ± 0.66
2.037IlePro: 2.037 ± 0.576
2.546IleGln: 2.546 ± 0.966
2.716IleArg: 2.716 ± 1.057
5.431IleSer: 5.431 ± 1.753
2.376IleThr: 2.376 ± 0.659
5.092IleVal: 5.092 ± 1.09
0.679IleTrp: 0.679 ± 0.192
1.527IleTyr: 1.527 ± 0.621
0.0IleXaa: 0.0 ± 0.0
Lys
4.582LysAla: 4.582 ± 0.258
1.018LysCys: 1.018 ± 0.359
5.261LysAsp: 5.261 ± 0.801
7.468LysGlu: 7.468 ± 2.267
2.716LysPhe: 2.716 ± 1.138
3.394LysGly: 3.394 ± 0.647
1.188LysHis: 1.188 ± 0.329
4.243LysIle: 4.243 ± 0.638
6.959LysLys: 6.959 ± 1.468
8.147LysLeu: 8.147 ± 1.562
1.867LysMet: 1.867 ± 0.628
2.885LysAsn: 2.885 ± 0.909
2.376LysPro: 2.376 ± 0.743
1.358LysGln: 1.358 ± 0.764
3.225LysArg: 3.225 ± 0.493
6.11LysSer: 6.11 ± 1.164
4.582LysThr: 4.582 ± 0.816
7.298LysVal: 7.298 ± 0.302
1.527LysTrp: 1.527 ± 0.811
1.867LysTyr: 1.867 ± 0.11
0.0LysXaa: 0.0 ± 0.0
Leu
2.885LeuAla: 2.885 ± 0.463
1.867LeuCys: 1.867 ± 0.11
5.431LeuAsp: 5.431 ± 1.103
6.28LeuGlu: 6.28 ± 0.624
4.752LeuPhe: 4.752 ± 0.565
3.564LeuGly: 3.564 ± 0.728
2.885LeuHis: 2.885 ± 0.301
6.449LeuIle: 6.449 ± 1.281
8.656LeuLys: 8.656 ± 0.231
10.523LeuLeu: 10.523 ± 1.038
2.376LeuMet: 2.376 ± 0.717
6.28LeuAsn: 6.28 ± 1.984
4.922LeuPro: 4.922 ± 0.311
4.243LeuGln: 4.243 ± 1.483
5.261LeuArg: 5.261 ± 0.479
10.183LeuSer: 10.183 ± 0.799
8.995LeuThr: 8.995 ± 0.893
6.11LeuVal: 6.11 ± 0.756
1.188LeuTrp: 1.188 ± 0.273
2.716LeuTyr: 2.716 ± 0.189
0.0LeuXaa: 0.0 ± 0.0
Met
1.697MetAla: 1.697 ± 0.775
0.509MetCys: 0.509 ± 0.44
1.188MetAsp: 1.188 ± 0.678
1.697MetGlu: 1.697 ± 0.869
1.018MetPhe: 1.018 ± 0.879
1.188MetGly: 1.188 ± 0.273
0.849MetHis: 0.849 ± 0.449
1.018MetIle: 1.018 ± 0.573
2.885MetLys: 2.885 ± 0.731
3.904MetLeu: 3.904 ± 1.365
1.018MetMet: 1.018 ± 0.573
1.188MetAsn: 1.188 ± 0.329
1.018MetPro: 1.018 ± 0.359
0.679MetGln: 0.679 ± 0.426
0.679MetArg: 0.679 ± 0.426
1.358MetSer: 1.358 ± 0.529
2.037MetThr: 2.037 ± 0.016
0.849MetVal: 0.849 ± 0.291
0.0MetTrp: 0.0 ± 0.0
0.849MetTyr: 0.849 ± 0.265
0.0MetXaa: 0.0 ± 0.0
Asn
2.037AsnAla: 2.037 ± 0.592
2.037AsnCys: 2.037 ± 0.95
1.358AsnAsp: 1.358 ± 0.304
2.546AsnGlu: 2.546 ± 1.134
2.376AsnPhe: 2.376 ± 1.052
2.716AsnGly: 2.716 ± 1.788
1.018AsnHis: 1.018 ± 0.573
3.055AsnIle: 3.055 ± 0.825
2.716AsnLys: 2.716 ± 1.325
5.94AsnLeu: 5.94 ± 1.021
0.679AsnMet: 0.679 ± 0.388
2.037AsnAsn: 2.037 ± 0.718
1.697AsnPro: 1.697 ± 0.415
1.697AsnGln: 1.697 ± 0.415
1.697AsnArg: 1.697 ± 1.234
5.431AsnSer: 5.431 ± 0.815
1.867AsnThr: 1.867 ± 0.519
3.564AsnVal: 3.564 ± 0.615
1.018AsnTrp: 1.018 ± 0.296
1.527AsnTyr: 1.527 ± 1.273
0.0AsnXaa: 0.0 ± 0.0
Pro
2.037ProAla: 2.037 ± 1.837
0.17ProCys: 0.17 ± 0.095
2.037ProAsp: 2.037 ± 0.31
2.376ProGlu: 2.376 ± 0.172
1.188ProPhe: 1.188 ± 0.282
1.527ProGly: 1.527 ± 0.835
0.849ProHis: 0.849 ± 0.291
2.716ProIle: 2.716 ± 0.858
2.376ProLys: 2.376 ± 0.546
1.867ProLeu: 1.867 ± 0.396
0.849ProMet: 0.849 ± 0.265
1.867ProAsn: 1.867 ± 0.708
0.679ProPro: 0.679 ± 0.944
0.679ProGln: 0.679 ± 0.192
0.849ProArg: 0.849 ± 0.265
3.564ProSer: 3.564 ± 1.312
3.055ProThr: 3.055 ± 0.602
2.885ProVal: 2.885 ± 0.331
0.679ProTrp: 0.679 ± 0.388
1.358ProTyr: 1.358 ± 0.434
0.0ProXaa: 0.0 ± 0.0
Gln
2.037GlnAla: 2.037 ± 0.277
1.188GlnCys: 1.188 ± 0.437
1.867GlnAsp: 1.867 ± 0.612
1.527GlnGlu: 1.527 ± 0.731
1.527GlnPhe: 1.527 ± 0.859
1.697GlnGly: 1.697 ± 0.473
0.849GlnHis: 0.849 ± 0.477
1.018GlnIle: 1.018 ± 0.349
2.716GlnLys: 2.716 ± 0.638
3.904GlnLeu: 3.904 ± 1.494
1.358GlnMet: 1.358 ± 0.304
0.849GlnAsn: 0.849 ± 0.683
1.018GlnPro: 1.018 ± 0.462
1.527GlnGln: 1.527 ± 0.631
2.376GlnArg: 2.376 ± 0.472
2.376GlnSer: 2.376 ± 1.043
1.697GlnThr: 1.697 ± 0.714
1.697GlnVal: 1.697 ± 0.485
0.0GlnTrp: 0.0 ± 0.0
0.339GlnTyr: 0.339 ± 0.472
0.0GlnXaa: 0.0 ± 0.0
Arg
2.376ArgAla: 2.376 ± 0.172
1.527ArgCys: 1.527 ± 0.453
1.697ArgAsp: 1.697 ± 0.955
2.546ArgGlu: 2.546 ± 0.832
2.037ArgPhe: 2.037 ± 0.31
3.225ArgGly: 3.225 ± 1.416
1.867ArgHis: 1.867 ± 0.396
1.867ArgIle: 1.867 ± 0.758
2.206ArgLys: 2.206 ± 0.631
5.771ArgLeu: 5.771 ± 1.215
1.188ArgMet: 1.188 ± 0.445
2.037ArgAsn: 2.037 ± 0.277
1.188ArgPro: 1.188 ± 0.492
0.679ArgGln: 0.679 ± 0.426
2.206ArgArg: 2.206 ± 0.523
3.904ArgSer: 3.904 ± 1.036
2.546ArgThr: 2.546 ± 0.153
3.055ArgVal: 3.055 ± 0.906
0.509ArgTrp: 0.509 ± 0.148
1.188ArgTyr: 1.188 ± 0.437
0.0ArgXaa: 0.0 ± 0.0
Ser
6.11SerAla: 6.11 ± 0.049
3.055SerCys: 3.055 ± 1.025
5.092SerAsp: 5.092 ± 1.459
5.601SerGlu: 5.601 ± 0.597
2.885SerPhe: 2.885 ± 0.992
5.431SerGly: 5.431 ± 0.758
1.527SerHis: 1.527 ± 0.144
5.601SerIle: 5.601 ± 0.442
7.637SerLys: 7.637 ± 0.346
10.014SerLeu: 10.014 ± 0.806
2.546SerMet: 2.546 ± 0.832
3.734SerAsn: 3.734 ± 1.478
2.376SerPro: 2.376 ± 0.447
3.225SerGln: 3.225 ± 0.919
2.037SerArg: 2.037 ± 0.592
11.711SerSer: 11.711 ± 1.269
5.431SerThr: 5.431 ± 1.371
4.413SerVal: 4.413 ± 1.854
1.018SerTrp: 1.018 ± 1.067
4.073SerTyr: 4.073 ± 1.184
0.0SerXaa: 0.0 ± 0.0
Thr
3.055ThrAla: 3.055 ± 1.025
1.697ThrCys: 1.697 ± 1.043
2.546ThrAsp: 2.546 ± 0.74
5.092ThrGlu: 5.092 ± 0.81
2.206ThrPhe: 2.206 ± 0.643
3.225ThrGly: 3.225 ± 0.456
1.527ThrHis: 1.527 ± 0.444
2.716ThrIle: 2.716 ± 0.419
3.225ThrLys: 3.225 ± 0.274
6.789ThrLeu: 6.789 ± 0.407
1.527ThrMet: 1.527 ± 0.453
3.055ThrAsn: 3.055 ± 0.37
2.716ThrPro: 2.716 ± 0.769
1.867ThrGln: 1.867 ± 0.612
1.697ThrArg: 1.697 ± 0.53
5.94ThrSer: 5.94 ± 0.668
3.394ThrThr: 3.394 ± 1.359
6.789ThrVal: 6.789 ± 1.101
1.188ThrTrp: 1.188 ± 0.946
1.697ThrTyr: 1.697 ± 0.206
0.0ThrXaa: 0.0 ± 0.0
Val
2.885ValAla: 2.885 ± 0.835
0.849ValCys: 0.849 ± 0.33
3.394ValAsp: 3.394 ± 0.647
6.449ValGlu: 6.449 ± 0.149
1.018ValPhe: 1.018 ± 0.292
1.867ValGly: 1.867 ± 0.396
1.358ValHis: 1.358 ± 0.887
4.243ValIle: 4.243 ± 0.775
7.637ValLys: 7.637 ± 0.816
8.656ValLeu: 8.656 ± 0.497
1.527ValMet: 1.527 ± 0.621
3.394ValAsn: 3.394 ± 0.685
2.376ValPro: 2.376 ± 1.602
2.206ValGln: 2.206 ± 0.724
2.716ValArg: 2.716 ± 0.876
7.637ValSer: 7.637 ± 0.144
4.243ValThr: 4.243 ± 0.068
5.261ValVal: 5.261 ± 0.899
0.339ValTrp: 0.339 ± 0.191
2.206ValTyr: 2.206 ± 0.523
0.0ValXaa: 0.0 ± 0.0
Trp
0.17TrpAla: 0.17 ± 0.216
0.509TrpCys: 0.509 ± 0.148
0.679TrpAsp: 0.679 ± 0.388
0.679TrpGlu: 0.679 ± 0.388
0.339TrpPhe: 0.339 ± 0.538
1.018TrpGly: 1.018 ± 0.85
0.509TrpHis: 0.509 ± 0.459
0.509TrpIle: 0.509 ± 0.286
1.358TrpLys: 1.358 ± 0.853
1.697TrpLeu: 1.697 ± 1.118
0.679TrpMet: 0.679 ± 0.576
0.679TrpAsn: 0.679 ± 0.317
0.509TrpPro: 0.509 ± 0.148
0.509TrpGln: 0.509 ± 0.647
0.339TrpArg: 0.339 ± 0.158
1.358TrpSer: 1.358 ± 0.434
0.849TrpThr: 0.849 ± 0.291
0.339TrpVal: 0.339 ± 0.191
0.17TrpTrp: 0.17 ± 0.095
0.339TrpTyr: 0.339 ± 0.472
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.358TyrAla: 1.358 ± 0.304
0.849TyrCys: 0.849 ± 0.291
1.697TyrAsp: 1.697 ± 0.146
1.358TyrGlu: 1.358 ± 0.777
2.206TyrPhe: 2.206 ± 1.151
1.358TyrGly: 1.358 ± 0.634
0.849TyrHis: 0.849 ± 0.795
2.376TyrIle: 2.376 ± 0.177
1.697TyrLys: 1.697 ± 1.299
3.055TyrLeu: 3.055 ± 0.558
1.018TyrMet: 1.018 ± 0.349
2.546TyrAsn: 2.546 ± 0.545
0.679TyrPro: 0.679 ± 0.382
1.188TyrGln: 1.188 ± 0.492
2.206TyrArg: 2.206 ± 0.916
2.716TyrSer: 2.716 ± 0.868
1.697TyrThr: 1.697 ± 0.731
1.697TyrVal: 1.697 ± 0.485
0.849TyrTrp: 0.849 ± 0.434
1.358TyrTyr: 1.358 ± 0.529
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3 proteins (5893 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski