Amino acid dipepetide frequency for Bacillus phage phIS3501

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.019AlaAla: 5.019 ± 1.004
0.717AlaCys: 0.717 ± 0.334
3.585AlaAsp: 3.585 ± 0.698
4.213AlaGlu: 4.213 ± 0.523
2.061AlaPhe: 2.061 ± 0.409
3.854AlaGly: 3.854 ± 0.807
0.896AlaHis: 0.896 ± 0.336
3.854AlaIle: 3.854 ± 0.645
5.916AlaLys: 5.916 ± 0.749
5.199AlaLeu: 5.199 ± 0.682
2.151AlaMet: 2.151 ± 0.409
3.585AlaAsn: 3.585 ± 0.805
1.434AlaPro: 1.434 ± 0.323
2.151AlaGln: 2.151 ± 0.416
2.689AlaArg: 2.689 ± 0.448
2.599AlaSer: 2.599 ± 0.675
4.661AlaThr: 4.661 ± 0.977
4.392AlaVal: 4.392 ± 0.778
0.717AlaTrp: 0.717 ± 0.261
1.972AlaTyr: 1.972 ± 0.382
0.0AlaXaa: 0.0 ± 0.0
Cys
0.448CysAla: 0.448 ± 0.216
0.09CysCys: 0.09 ± 0.089
0.807CysAsp: 0.807 ± 0.248
0.627CysGlu: 0.627 ± 0.236
0.448CysPhe: 0.448 ± 0.307
0.359CysGly: 0.359 ± 0.176
0.448CysHis: 0.448 ± 0.233
0.359CysIle: 0.359 ± 0.196
0.538CysLys: 0.538 ± 0.239
0.09CysLeu: 0.09 ± 0.094
0.09CysMet: 0.09 ± 0.091
0.179CysAsn: 0.179 ± 0.118
0.538CysPro: 0.538 ± 0.217
0.359CysGln: 0.359 ± 0.173
0.359CysArg: 0.359 ± 0.197
0.627CysSer: 0.627 ± 0.21
0.717CysThr: 0.717 ± 0.25
0.448CysVal: 0.448 ± 0.207
0.0CysTrp: 0.0 ± 0.0
0.359CysTyr: 0.359 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
3.227AspAla: 3.227 ± 0.688
0.179AspCys: 0.179 ± 0.129
3.316AspAsp: 3.316 ± 0.605
4.213AspGlu: 4.213 ± 0.533
1.524AspPhe: 1.524 ± 0.369
3.496AspGly: 3.496 ± 0.562
0.627AspHis: 0.627 ± 0.238
4.302AspIle: 4.302 ± 0.653
6.095AspLys: 6.095 ± 0.652
4.75AspLeu: 4.75 ± 0.687
2.689AspMet: 2.689 ± 0.389
2.779AspAsn: 2.779 ± 0.554
1.793AspPro: 1.793 ± 0.418
1.255AspGln: 1.255 ± 0.33
2.33AspArg: 2.33 ± 0.52
2.599AspSer: 2.599 ± 0.437
2.599AspThr: 2.599 ± 0.392
4.033AspVal: 4.033 ± 0.522
0.717AspTrp: 0.717 ± 0.216
2.779AspTyr: 2.779 ± 0.538
0.0AspXaa: 0.0 ± 0.0
Glu
5.916GluAla: 5.916 ± 0.638
0.627GluCys: 0.627 ± 0.25
4.661GluAsp: 4.661 ± 0.805
7.35GluGlu: 7.35 ± 1.038
3.944GluPhe: 3.944 ± 0.702
4.571GluGly: 4.571 ± 0.646
1.344GluHis: 1.344 ± 0.38
6.812GluIle: 6.812 ± 0.753
8.156GluLys: 8.156 ± 0.691
7.887GluLeu: 7.887 ± 0.618
2.241GluMet: 2.241 ± 0.415
3.854GluAsn: 3.854 ± 0.524
1.703GluPro: 1.703 ± 0.612
5.199GluGln: 5.199 ± 0.58
3.675GluArg: 3.675 ± 0.713
4.033GluSer: 4.033 ± 0.709
4.392GluThr: 4.392 ± 0.59
4.661GluVal: 4.661 ± 0.535
0.896GluTrp: 0.896 ± 0.306
2.599GluTyr: 2.599 ± 0.584
0.0GluXaa: 0.0 ± 0.0
Phe
1.793PheAla: 1.793 ± 0.374
0.448PheCys: 0.448 ± 0.263
2.599PheAsp: 2.599 ± 0.544
2.689PheGlu: 2.689 ± 0.442
1.703PhePhe: 1.703 ± 0.44
2.868PheGly: 2.868 ± 0.49
0.538PheHis: 0.538 ± 0.204
3.227PheIle: 3.227 ± 0.603
4.123PheLys: 4.123 ± 0.63
3.137PheLeu: 3.137 ± 0.657
1.344PheMet: 1.344 ± 0.347
3.316PheAsn: 3.316 ± 0.501
0.717PhePro: 0.717 ± 0.256
1.165PheGln: 1.165 ± 0.251
1.793PheArg: 1.793 ± 0.38
2.689PheSer: 2.689 ± 0.494
2.51PheThr: 2.51 ± 0.645
1.972PheVal: 1.972 ± 0.36
0.448PheTrp: 0.448 ± 0.226
1.613PheTyr: 1.613 ± 0.349
0.0PheXaa: 0.0 ± 0.0
Gly
2.51GlyAla: 2.51 ± 0.656
0.538GlyCys: 0.538 ± 0.284
2.51GlyAsp: 2.51 ± 0.371
5.826GlyGlu: 5.826 ± 0.654
2.958GlyPhe: 2.958 ± 0.583
2.868GlyGly: 2.868 ± 0.559
0.627GlyHis: 0.627 ± 0.217
4.213GlyIle: 4.213 ± 0.625
5.109GlyLys: 5.109 ± 0.668
4.302GlyLeu: 4.302 ± 0.773
2.241GlyMet: 2.241 ± 0.608
2.599GlyAsn: 2.599 ± 0.462
0.717GlyPro: 0.717 ± 0.228
1.793GlyGln: 1.793 ± 0.338
2.868GlyArg: 2.868 ± 0.451
3.137GlySer: 3.137 ± 0.513
2.599GlyThr: 2.599 ± 0.477
3.227GlyVal: 3.227 ± 0.564
1.255GlyTrp: 1.255 ± 0.382
2.599GlyTyr: 2.599 ± 0.395
0.0GlyXaa: 0.0 ± 0.0
His
0.717HisAla: 0.717 ± 0.282
0.269HisCys: 0.269 ± 0.145
0.986HisAsp: 0.986 ± 0.32
1.524HisGlu: 1.524 ± 0.334
0.538HisPhe: 0.538 ± 0.28
1.076HisGly: 1.076 ± 0.275
0.179HisHis: 0.179 ± 0.128
1.524HisIle: 1.524 ± 0.36
1.165HisLys: 1.165 ± 0.388
2.061HisLeu: 2.061 ± 0.495
0.717HisMet: 0.717 ± 0.274
0.448HisAsn: 0.448 ± 0.187
0.448HisPro: 0.448 ± 0.251
0.448HisGln: 0.448 ± 0.21
0.717HisArg: 0.717 ± 0.249
1.613HisSer: 1.613 ± 0.498
0.896HisThr: 0.896 ± 0.326
0.986HisVal: 0.986 ± 0.326
0.179HisTrp: 0.179 ± 0.139
0.448HisTyr: 0.448 ± 0.23
0.0HisXaa: 0.0 ± 0.0
Ile
6.095IleAla: 6.095 ± 0.616
0.269IleCys: 0.269 ± 0.124
3.316IleAsp: 3.316 ± 0.606
7.081IleGlu: 7.081 ± 0.59
2.689IlePhe: 2.689 ± 0.392
3.406IleGly: 3.406 ± 0.642
1.524IleHis: 1.524 ± 0.374
5.199IleIle: 5.199 ± 0.74
6.274IleLys: 6.274 ± 0.717
5.647IleLeu: 5.647 ± 0.786
2.51IleMet: 2.51 ± 0.523
5.916IleAsn: 5.916 ± 0.977
2.061IlePro: 2.061 ± 0.409
3.675IleGln: 3.675 ± 0.517
4.392IleArg: 4.392 ± 0.683
4.213IleSer: 4.213 ± 0.723
3.764IleThr: 3.764 ± 0.595
4.123IleVal: 4.123 ± 0.507
0.448IleTrp: 0.448 ± 0.261
2.42IleTyr: 2.42 ± 0.447
0.0IleXaa: 0.0 ± 0.0
Lys
5.916LysAla: 5.916 ± 0.782
0.627LysCys: 0.627 ± 0.303
4.93LysAsp: 4.93 ± 0.633
8.067LysGlu: 8.067 ± 0.579
3.944LysPhe: 3.944 ± 0.543
4.571LysGly: 4.571 ± 0.547
1.613LysHis: 1.613 ± 0.399
6.812LysIle: 6.812 ± 0.582
8.246LysLys: 8.246 ± 0.911
6.274LysLeu: 6.274 ± 0.661
2.958LysMet: 2.958 ± 0.597
5.736LysAsn: 5.736 ± 0.618
2.241LysPro: 2.241 ± 0.564
5.557LysGln: 5.557 ± 0.967
5.019LysArg: 5.019 ± 0.608
4.93LysSer: 4.93 ± 0.46
6.095LysThr: 6.095 ± 0.622
5.467LysVal: 5.467 ± 0.68
1.165LysTrp: 1.165 ± 0.343
3.227LysTyr: 3.227 ± 0.518
0.0LysXaa: 0.0 ± 0.0
Leu
4.302LeuAla: 4.302 ± 0.534
0.807LeuCys: 0.807 ± 0.312
4.392LeuAsp: 4.392 ± 0.626
6.901LeuGlu: 6.901 ± 1.014
2.51LeuPhe: 2.51 ± 0.539
4.123LeuGly: 4.123 ± 0.771
1.613LeuHis: 1.613 ± 0.442
4.481LeuIle: 4.481 ± 0.585
9.232LeuLys: 9.232 ± 0.739
5.109LeuLeu: 5.109 ± 0.967
1.703LeuMet: 1.703 ± 0.345
5.378LeuAsn: 5.378 ± 0.797
2.599LeuPro: 2.599 ± 0.428
3.496LeuGln: 3.496 ± 0.552
3.585LeuArg: 3.585 ± 0.508
5.916LeuSer: 5.916 ± 0.767
4.481LeuThr: 4.481 ± 0.63
4.481LeuVal: 4.481 ± 0.622
0.896LeuTrp: 0.896 ± 0.298
2.599LeuTyr: 2.599 ± 0.598
0.0LeuXaa: 0.0 ± 0.0
Met
2.241MetAla: 2.241 ± 0.443
0.179MetCys: 0.179 ± 0.124
1.703MetAsp: 1.703 ± 0.419
2.42MetGlu: 2.42 ± 0.345
0.448MetPhe: 0.448 ± 0.241
1.793MetGly: 1.793 ± 0.462
0.538MetHis: 0.538 ± 0.238
1.882MetIle: 1.882 ± 0.501
3.764MetLys: 3.764 ± 0.639
2.42MetLeu: 2.42 ± 0.646
0.896MetMet: 0.896 ± 0.283
2.151MetAsn: 2.151 ± 0.54
0.807MetPro: 0.807 ± 0.238
1.703MetGln: 1.703 ± 0.431
1.524MetArg: 1.524 ± 0.333
1.793MetSer: 1.793 ± 0.394
1.793MetThr: 1.793 ± 0.349
1.165MetVal: 1.165 ± 0.342
0.538MetTrp: 0.538 ± 0.18
0.538MetTyr: 0.538 ± 0.265
0.0MetXaa: 0.0 ± 0.0
Asn
4.392AsnAla: 4.392 ± 0.637
0.269AsnCys: 0.269 ± 0.183
2.33AsnAsp: 2.33 ± 0.445
5.378AsnGlu: 5.378 ± 0.54
1.703AsnPhe: 1.703 ± 0.448
3.406AsnGly: 3.406 ± 0.576
1.076AsnHis: 1.076 ± 0.25
4.481AsnIle: 4.481 ± 0.636
6.543AsnLys: 6.543 ± 0.954
3.496AsnLeu: 3.496 ± 0.732
1.344AsnMet: 1.344 ± 0.322
2.958AsnAsn: 2.958 ± 0.386
2.241AsnPro: 2.241 ± 0.557
2.51AsnGln: 2.51 ± 0.393
3.316AsnArg: 3.316 ± 0.522
3.496AsnSer: 3.496 ± 0.398
3.675AsnThr: 3.675 ± 0.475
3.675AsnVal: 3.675 ± 0.518
0.448AsnTrp: 0.448 ± 0.165
2.151AsnTyr: 2.151 ± 0.394
0.0AsnXaa: 0.0 ± 0.0
Pro
1.165ProAla: 1.165 ± 0.328
0.269ProCys: 0.269 ± 0.152
1.165ProAsp: 1.165 ± 0.288
2.42ProGlu: 2.42 ± 0.469
0.896ProPhe: 0.896 ± 0.327
1.344ProGly: 1.344 ± 0.308
0.717ProHis: 0.717 ± 0.232
2.868ProIle: 2.868 ± 0.615
1.255ProLys: 1.255 ± 0.384
2.42ProLeu: 2.42 ± 0.427
0.538ProMet: 0.538 ± 0.202
1.434ProAsn: 1.434 ± 0.36
0.807ProPro: 0.807 ± 0.31
0.986ProGln: 0.986 ± 0.429
0.896ProArg: 0.896 ± 0.271
2.151ProSer: 2.151 ± 0.396
2.42ProThr: 2.42 ± 0.538
1.613ProVal: 1.613 ± 0.512
0.359ProTrp: 0.359 ± 0.183
2.061ProTyr: 2.061 ± 0.423
0.0ProXaa: 0.0 ± 0.0
Gln
3.047GlnAla: 3.047 ± 0.739
0.269GlnCys: 0.269 ± 0.149
1.972GlnAsp: 1.972 ± 0.431
3.227GlnGlu: 3.227 ± 0.705
1.972GlnPhe: 1.972 ± 0.5
2.241GlnGly: 2.241 ± 0.487
0.717GlnHis: 0.717 ± 0.329
3.137GlnIle: 3.137 ± 0.507
4.571GlnLys: 4.571 ± 0.632
3.316GlnLeu: 3.316 ± 0.596
1.524GlnMet: 1.524 ± 0.51
3.227GlnAsn: 3.227 ± 0.485
1.613GlnPro: 1.613 ± 0.432
2.689GlnGln: 2.689 ± 0.494
1.793GlnArg: 1.793 ± 0.389
2.061GlnSer: 2.061 ± 0.463
1.703GlnThr: 1.703 ± 0.29
3.047GlnVal: 3.047 ± 0.608
0.448GlnTrp: 0.448 ± 0.174
1.882GlnTyr: 1.882 ± 0.36
0.0GlnXaa: 0.0 ± 0.0
Arg
2.51ArgAla: 2.51 ± 0.547
0.269ArgCys: 0.269 ± 0.179
3.047ArgAsp: 3.047 ± 0.445
3.854ArgGlu: 3.854 ± 0.655
2.151ArgPhe: 2.151 ± 0.357
2.42ArgGly: 2.42 ± 0.411
0.627ArgHis: 0.627 ± 0.233
4.213ArgIle: 4.213 ± 0.592
4.213ArgLys: 4.213 ± 0.54
4.123ArgLeu: 4.123 ± 0.639
1.344ArgMet: 1.344 ± 0.274
2.151ArgAsn: 2.151 ± 0.501
1.076ArgPro: 1.076 ± 0.33
2.779ArgGln: 2.779 ± 0.598
1.882ArgArg: 1.882 ± 0.371
1.882ArgSer: 1.882 ± 0.445
1.793ArgThr: 1.793 ± 0.358
2.779ArgVal: 2.779 ± 0.5
0.538ArgTrp: 0.538 ± 0.158
1.524ArgTyr: 1.524 ± 0.321
0.0ArgXaa: 0.0 ± 0.0
Ser
2.599SerAla: 2.599 ± 0.739
0.269SerCys: 0.269 ± 0.2
3.854SerAsp: 3.854 ± 0.537
4.302SerGlu: 4.302 ± 0.568
2.958SerPhe: 2.958 ± 0.551
2.868SerGly: 2.868 ± 0.523
1.344SerHis: 1.344 ± 0.491
5.199SerIle: 5.199 ± 0.512
5.109SerLys: 5.109 ± 0.668
4.302SerLeu: 4.302 ± 0.646
1.524SerMet: 1.524 ± 0.404
3.496SerAsn: 3.496 ± 0.519
1.344SerPro: 1.344 ± 0.361
2.151SerGln: 2.151 ± 0.432
2.599SerArg: 2.599 ± 0.55
3.496SerSer: 3.496 ± 0.52
2.958SerThr: 2.958 ± 0.556
3.944SerVal: 3.944 ± 0.745
0.269SerTrp: 0.269 ± 0.148
1.793SerTyr: 1.793 ± 0.332
0.0SerXaa: 0.0 ± 0.0
Thr
3.406ThrAla: 3.406 ± 0.704
0.627ThrCys: 0.627 ± 0.224
3.047ThrAsp: 3.047 ± 0.58
4.661ThrGlu: 4.661 ± 0.592
3.227ThrPhe: 3.227 ± 0.48
3.764ThrGly: 3.764 ± 0.524
0.986ThrHis: 0.986 ± 0.308
3.406ThrIle: 3.406 ± 0.529
4.661ThrLys: 4.661 ± 0.7
6.095ThrLeu: 6.095 ± 0.664
1.076ThrMet: 1.076 ± 0.295
3.406ThrAsn: 3.406 ± 0.723
2.151ThrPro: 2.151 ± 0.504
1.434ThrGln: 1.434 ± 0.316
1.344ThrArg: 1.344 ± 0.317
3.406ThrSer: 3.406 ± 0.392
4.213ThrThr: 4.213 ± 0.62
4.213ThrVal: 4.213 ± 0.515
0.717ThrTrp: 0.717 ± 0.237
2.151ThrTyr: 2.151 ± 0.558
0.0ThrXaa: 0.0 ± 0.0
Val
3.764ValAla: 3.764 ± 0.584
0.359ValCys: 0.359 ± 0.171
4.571ValAsp: 4.571 ± 0.644
5.557ValGlu: 5.557 ± 0.913
2.241ValPhe: 2.241 ± 0.429
3.227ValGly: 3.227 ± 0.463
0.627ValHis: 0.627 ± 0.262
4.84ValIle: 4.84 ± 0.659
4.302ValLys: 4.302 ± 0.615
4.123ValLeu: 4.123 ± 0.641
2.33ValMet: 2.33 ± 0.414
3.496ValAsn: 3.496 ± 0.507
2.51ValPro: 2.51 ± 0.517
2.51ValGln: 2.51 ± 0.553
1.972ValArg: 1.972 ± 0.455
3.585ValSer: 3.585 ± 0.464
3.854ValThr: 3.854 ± 0.519
3.585ValVal: 3.585 ± 0.705
0.986ValTrp: 0.986 ± 0.258
3.047ValTyr: 3.047 ± 0.598
0.0ValXaa: 0.0 ± 0.0
Trp
0.538TrpAla: 0.538 ± 0.217
0.0TrpCys: 0.0 ± 0.0
0.986TrpAsp: 0.986 ± 0.24
1.255TrpGlu: 1.255 ± 0.286
0.538TrpPhe: 0.538 ± 0.218
0.538TrpGly: 0.538 ± 0.283
0.179TrpHis: 0.179 ± 0.121
0.807TrpIle: 0.807 ± 0.251
0.807TrpLys: 0.807 ± 0.252
1.165TrpLeu: 1.165 ± 0.297
0.179TrpMet: 0.179 ± 0.182
0.717TrpAsn: 0.717 ± 0.211
0.0TrpPro: 0.0 ± 0.0
0.538TrpGln: 0.538 ± 0.213
0.538TrpArg: 0.538 ± 0.196
0.896TrpSer: 0.896 ± 0.237
0.538TrpThr: 0.538 ± 0.2
0.717TrpVal: 0.717 ± 0.324
0.0TrpTrp: 0.0 ± 0.0
0.538TrpTyr: 0.538 ± 0.197
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.061TyrAla: 2.061 ± 0.504
0.807TyrCys: 0.807 ± 0.274
1.524TyrAsp: 1.524 ± 0.341
2.958TyrGlu: 2.958 ± 0.405
2.241TyrPhe: 2.241 ± 0.458
1.703TyrGly: 1.703 ± 0.359
0.717TyrHis: 0.717 ± 0.3
3.406TyrIle: 3.406 ± 0.665
3.316TyrLys: 3.316 ± 0.625
2.689TyrLeu: 2.689 ± 0.531
0.807TyrMet: 0.807 ± 0.268
2.061TyrAsn: 2.061 ± 0.455
1.076TyrPro: 1.076 ± 0.358
2.061TyrGln: 2.061 ± 0.463
1.882TyrArg: 1.882 ± 0.356
1.255TyrSer: 1.255 ± 0.443
2.33TyrThr: 2.33 ± 0.509
2.958TyrVal: 2.958 ± 0.437
0.448TyrTrp: 0.448 ± 0.185
1.255TyrTyr: 1.255 ± 0.473
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (11158 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski