Isoptericola sp. CG 20/1183

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Promicromonosporaceae; Isoptericola; unclassified Isoptericola

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3485 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0Y435|A0A2T0Y435_9MICO Xanthine dehydrogenase accessory factor OS=Isoptericola sp. CG 20/1183 OX=1881052 GN=BCE75_104201 PE=4 SV=1
MM1 pKa = 8.1RR2 pKa = 11.84SRR4 pKa = 11.84SLRR7 pKa = 11.84PRR9 pKa = 11.84DD10 pKa = 3.48EE11 pKa = 4.23AVSRR15 pKa = 11.84PVAVAATLALAAVPLLGATTTASAAEE41 pKa = 4.27RR42 pKa = 11.84VDD44 pKa = 3.59NPYY47 pKa = 10.81VGADD51 pKa = 3.39QYY53 pKa = 11.91VNSYY57 pKa = 8.45WSEE60 pKa = 4.05NVVDD64 pKa = 4.78AAAEE68 pKa = 4.08AGGDD72 pKa = 3.87LGQQMLTLADD82 pKa = 3.94TPTSVWMDD90 pKa = 3.21RR91 pKa = 11.84TSAIEE96 pKa = 4.06GNVDD100 pKa = 3.6GPGLRR105 pKa = 11.84YY106 pKa = 9.84HH107 pKa = 7.37LDD109 pKa = 3.34AALEE113 pKa = 4.08QQQGDD118 pKa = 3.64TPMVFNLVIYY128 pKa = 8.83NLPGRR133 pKa = 11.84DD134 pKa = 3.75CYY136 pKa = 11.63ALASNGLLDD145 pKa = 3.93PTPEE149 pKa = 4.13GMAEE153 pKa = 4.08YY154 pKa = 9.99KK155 pKa = 10.52NEE157 pKa = 4.42YY158 pKa = 9.3IDD160 pKa = 5.26VIADD164 pKa = 3.51MLAEE168 pKa = 4.11PQYY171 pKa = 11.69ADD173 pKa = 3.58LRR175 pKa = 11.84VVATIEE181 pKa = 4.21PDD183 pKa = 3.36SLPNLVTNMSEE194 pKa = 4.43PKK196 pKa = 9.45CQEE199 pKa = 3.48SAPYY203 pKa = 9.47YY204 pKa = 10.45RR205 pKa = 11.84EE206 pKa = 3.89GTAYY210 pKa = 10.45ALDD213 pKa = 3.72RR214 pKa = 11.84LYY216 pKa = 11.08EE217 pKa = 4.26AGNVYY222 pKa = 10.54SYY224 pKa = 11.27IDD226 pKa = 3.57AAHH229 pKa = 6.66AGWLGWDD236 pKa = 3.86SNAGPAVDD244 pKa = 3.87VFQEE248 pKa = 4.49VVTSTEE254 pKa = 3.54HH255 pKa = 7.32GYY257 pKa = 8.65DD258 pKa = 3.3TVAGFVTNTANSTPLHH274 pKa = 6.05EE275 pKa = 5.43PFLEE279 pKa = 4.42DD280 pKa = 3.75EE281 pKa = 4.74PFGSGGGTQVRR292 pKa = 11.84QADD295 pKa = 5.09FYY297 pKa = 11.35QWNSDD302 pKa = 3.31FDD304 pKa = 3.95EE305 pKa = 5.15LDD307 pKa = 3.4WTEE310 pKa = 4.25HH311 pKa = 6.16LYY313 pKa = 11.21SLAVAAGFPSDD324 pKa = 3.31IGMLIDD330 pKa = 3.66TSRR333 pKa = 11.84NGWGGPDD340 pKa = 3.21RR341 pKa = 11.84PTAEE345 pKa = 4.48SSSSTLNTYY354 pKa = 9.97VDD356 pKa = 3.67EE357 pKa = 4.55SRR359 pKa = 11.84VDD361 pKa = 3.34RR362 pKa = 11.84RR363 pKa = 11.84THH365 pKa = 6.28RR366 pKa = 11.84GAWCNPAGAGLGEE379 pKa = 4.53RR380 pKa = 11.84PTVNPTDD387 pKa = 3.8NPASHH392 pKa = 7.3LDD394 pKa = 3.17AYY396 pKa = 10.95VWVKK400 pKa = 10.59PPGEE404 pKa = 3.89SDD406 pKa = 3.57GNSEE410 pKa = 5.58EE411 pKa = 4.44IPNDD415 pKa = 3.09QGKK418 pKa = 9.72SFDD421 pKa = 4.04RR422 pKa = 11.84MCDD425 pKa = 3.25PTFASPKK432 pKa = 10.1LDD434 pKa = 3.43GKK436 pKa = 9.43LTGALGGAPLAGQWFQDD453 pKa = 3.52QFEE456 pKa = 4.42MLVTNAYY463 pKa = 9.28PPIDD467 pKa = 4.56GGSVDD472 pKa = 5.2PDD474 pKa = 3.82TEE476 pKa = 4.66APTAPADD483 pKa = 3.7LAATGTTSTSTDD495 pKa = 3.78LTWGASSDD503 pKa = 3.76DD504 pKa = 3.84TGVTRR509 pKa = 11.84YY510 pKa = 7.66TVLVDD515 pKa = 3.36GAEE518 pKa = 4.4AGTSATTSYY527 pKa = 10.03TLTGLTPSTTYY538 pKa = 10.58EE539 pKa = 4.08VTVTARR545 pKa = 11.84DD546 pKa = 3.28AAGNVSAASAPVTVTTKK563 pKa = 10.32EE564 pKa = 3.92ATGGEE569 pKa = 4.35DD570 pKa = 3.21TQAPTVPTGLAAGEE584 pKa = 4.39VTSSSVEE591 pKa = 4.18LTWAAADD598 pKa = 4.54DD599 pKa = 4.06DD600 pKa = 4.57TGVAGYY606 pKa = 9.65RR607 pKa = 11.84VHH609 pKa = 7.85RR610 pKa = 11.84DD611 pKa = 2.74GDD613 pKa = 3.88LVADD617 pKa = 3.91VTGTSATDD625 pKa = 2.96SGLAADD631 pKa = 4.02TAYY634 pKa = 10.83SYY636 pKa = 10.75TVTAYY641 pKa = 10.27DD642 pKa = 3.37AAGNASAASDD652 pKa = 3.72ALSVTTEE659 pKa = 4.04EE660 pKa = 5.34GGTTPTGGCTVDD672 pKa = 3.41YY673 pKa = 10.81SANSWNTGFTGSFVVTNDD691 pKa = 2.42SDD693 pKa = 3.85AAIDD697 pKa = 3.68GWEE700 pKa = 4.11LTFTFADD707 pKa = 4.29GQTVDD712 pKa = 4.58QIWSAQASQSGSAVTITPAAWSTTIPAGGSVTFGFNGSHH751 pKa = 6.53SGTNTAPDD759 pKa = 4.07DD760 pKa = 3.8FALDD764 pKa = 4.06GVACDD769 pKa = 3.63IAA771 pKa = 5.89

Molecular weight: 80.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0Y6M9|A0A2T0Y6M9_9MICO Isochorismate synthase OS=Isoptericola sp. CG 20/1183 OX=1881052 GN=BCE75_102398 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3485

0

3485

1184991

30

3095

340.0

36.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.14 ± 0.063

0.544 ± 0.01

6.776 ± 0.043

5.703 ± 0.037

2.555 ± 0.024

9.474 ± 0.038

2.166 ± 0.02

2.797 ± 0.026

1.402 ± 0.027

10.068 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.611 ± 0.015

1.482 ± 0.022

5.889 ± 0.035

2.604 ± 0.021

7.842 ± 0.053

4.99 ± 0.03

6.366 ± 0.033

10.15 ± 0.043

1.59 ± 0.019

1.851 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski