Amino acid dipepetide frequency for Mycobacterium phage Hutc2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.069AlaAla: 12.069 ± 1.147
0.688AlaCys: 0.688 ± 0.189
6.191AlaAsp: 6.191 ± 0.629
7.566AlaGlu: 7.566 ± 0.732
3.627AlaPhe: 3.627 ± 0.497
7.191AlaGly: 7.191 ± 0.833
1.438AlaHis: 1.438 ± 0.275
5.065AlaIle: 5.065 ± 0.588
4.065AlaLys: 4.065 ± 0.675
8.567AlaLeu: 8.567 ± 1.163
2.564AlaMet: 2.564 ± 0.424
3.439AlaAsn: 3.439 ± 0.594
4.627AlaPro: 4.627 ± 0.665
3.252AlaGln: 3.252 ± 0.531
6.003AlaArg: 6.003 ± 0.56
4.19AlaSer: 4.19 ± 0.474
5.128AlaThr: 5.128 ± 0.682
6.753AlaVal: 6.753 ± 0.678
1.876AlaTrp: 1.876 ± 0.293
2.439AlaTyr: 2.439 ± 0.437
0.0AlaXaa: 0.0 ± 0.0
Cys
0.75CysAla: 0.75 ± 0.207
0.0CysCys: 0.0 ± 0.0
0.688CysAsp: 0.688 ± 0.197
0.313CysGlu: 0.313 ± 0.147
0.313CysPhe: 0.313 ± 0.162
0.875CysGly: 0.875 ± 0.25
0.188CysHis: 0.188 ± 0.108
0.5CysIle: 0.5 ± 0.208
0.188CysLys: 0.188 ± 0.108
0.75CysLeu: 0.75 ± 0.195
0.313CysMet: 0.313 ± 0.147
0.313CysAsn: 0.313 ± 0.127
0.375CysPro: 0.375 ± 0.223
0.313CysGln: 0.313 ± 0.127
0.938CysArg: 0.938 ± 0.243
0.313CysSer: 0.313 ± 0.133
0.438CysThr: 0.438 ± 0.162
0.5CysVal: 0.5 ± 0.189
0.313CysTrp: 0.313 ± 0.153
0.25CysTyr: 0.25 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
5.44AspAla: 5.44 ± 0.585
0.625AspCys: 0.625 ± 0.259
3.377AspAsp: 3.377 ± 0.429
4.627AspGlu: 4.627 ± 0.491
2.751AspPhe: 2.751 ± 0.539
5.628AspGly: 5.628 ± 0.682
1.938AspHis: 1.938 ± 0.442
3.314AspIle: 3.314 ± 0.455
3.002AspLys: 3.002 ± 0.393
5.44AspLeu: 5.44 ± 0.552
1.688AspMet: 1.688 ± 0.312
1.563AspAsn: 1.563 ± 0.338
4.815AspPro: 4.815 ± 0.506
2.126AspGln: 2.126 ± 0.35
4.002AspArg: 4.002 ± 0.532
3.002AspSer: 3.002 ± 0.422
3.939AspThr: 3.939 ± 0.442
4.815AspVal: 4.815 ± 0.57
1.251AspTrp: 1.251 ± 0.34
2.251AspTyr: 2.251 ± 0.406
0.0AspXaa: 0.0 ± 0.0
Glu
7.379GluAla: 7.379 ± 0.726
0.375GluCys: 0.375 ± 0.163
4.065GluAsp: 4.065 ± 0.509
6.253GluGlu: 6.253 ± 0.715
2.626GluPhe: 2.626 ± 0.44
6.253GluGly: 6.253 ± 0.596
2.126GluHis: 2.126 ± 0.451
3.939GluIle: 3.939 ± 0.511
2.439GluLys: 2.439 ± 0.364
7.691GluLeu: 7.691 ± 0.7
2.314GluMet: 2.314 ± 0.376
2.064GluAsn: 2.064 ± 0.343
2.689GluPro: 2.689 ± 0.492
2.064GluGln: 2.064 ± 0.353
4.627GluArg: 4.627 ± 0.585
2.751GluSer: 2.751 ± 0.38
3.689GluThr: 3.689 ± 0.384
4.94GluVal: 4.94 ± 0.623
1.501GluTrp: 1.501 ± 0.276
2.064GluTyr: 2.064 ± 0.395
0.0GluXaa: 0.0 ± 0.0
Phe
2.501PheAla: 2.501 ± 0.406
0.375PheCys: 0.375 ± 0.168
2.939PheAsp: 2.939 ± 0.442
2.626PheGlu: 2.626 ± 0.436
0.563PhePhe: 0.563 ± 0.207
2.814PheGly: 2.814 ± 0.46
0.5PheHis: 0.5 ± 0.185
1.501PheIle: 1.501 ± 0.293
1.501PheLys: 1.501 ± 0.304
2.564PheLeu: 2.564 ± 0.438
0.563PheMet: 0.563 ± 0.185
1.501PheAsn: 1.501 ± 0.317
2.501PhePro: 2.501 ± 0.367
0.938PheGln: 0.938 ± 0.236
2.251PheArg: 2.251 ± 0.395
2.314PheSer: 2.314 ± 0.434
1.563PheThr: 1.563 ± 0.314
2.564PheVal: 2.564 ± 0.464
0.438PheTrp: 0.438 ± 0.156
0.688PheTyr: 0.688 ± 0.215
0.0PheXaa: 0.0 ± 0.0
Gly
6.378GlyAla: 6.378 ± 0.854
0.5GlyCys: 0.5 ± 0.171
5.253GlyAsp: 5.253 ± 0.704
4.94GlyGlu: 4.94 ± 0.563
2.501GlyPhe: 2.501 ± 0.355
8.442GlyGly: 8.442 ± 1.484
2.251GlyHis: 2.251 ± 0.433
4.377GlyIle: 4.377 ± 0.527
4.127GlyLys: 4.127 ± 0.606
6.378GlyLeu: 6.378 ± 0.732
1.688GlyMet: 1.688 ± 0.274
2.751GlyAsn: 2.751 ± 0.508
3.689GlyPro: 3.689 ± 0.474
3.064GlyGln: 3.064 ± 0.478
3.752GlyArg: 3.752 ± 0.451
4.19GlySer: 4.19 ± 0.551
4.752GlyThr: 4.752 ± 0.57
5.69GlyVal: 5.69 ± 0.74
2.001GlyTrp: 2.001 ± 0.399
2.626GlyTyr: 2.626 ± 0.454
0.0GlyXaa: 0.0 ± 0.0
His
1.251HisAla: 1.251 ± 0.245
0.313HisCys: 0.313 ± 0.149
1.626HisAsp: 1.626 ± 0.403
1.626HisGlu: 1.626 ± 0.336
0.625HisPhe: 0.625 ± 0.243
1.751HisGly: 1.751 ± 0.343
0.75HisHis: 0.75 ± 0.182
1.063HisIle: 1.063 ± 0.264
1.188HisLys: 1.188 ± 0.335
1.626HisLeu: 1.626 ± 0.329
0.5HisMet: 0.5 ± 0.167
0.5HisAsn: 0.5 ± 0.157
1.001HisPro: 1.001 ± 0.247
1.126HisGln: 1.126 ± 0.329
2.064HisArg: 2.064 ± 0.354
0.75HisSer: 0.75 ± 0.214
1.063HisThr: 1.063 ± 0.242
1.813HisVal: 1.813 ± 0.376
0.438HisTrp: 0.438 ± 0.161
0.563HisTyr: 0.563 ± 0.249
0.0HisXaa: 0.0 ± 0.0
Ile
4.44IleAla: 4.44 ± 0.522
0.563IleCys: 0.563 ± 0.198
3.627IleAsp: 3.627 ± 0.452
5.378IleGlu: 5.378 ± 0.556
1.313IlePhe: 1.313 ± 0.265
3.564IleGly: 3.564 ± 0.415
1.001IleHis: 1.001 ± 0.248
2.126IleIle: 2.126 ± 0.432
2.314IleLys: 2.314 ± 0.392
3.127IleLeu: 3.127 ± 0.531
0.875IleMet: 0.875 ± 0.227
2.501IleAsn: 2.501 ± 0.415
3.814IlePro: 3.814 ± 0.481
1.376IleGln: 1.376 ± 0.275
3.814IleArg: 3.814 ± 0.486
2.751IleSer: 2.751 ± 0.422
3.252IleThr: 3.252 ± 0.398
3.189IleVal: 3.189 ± 0.423
0.625IleTrp: 0.625 ± 0.173
0.813IleTyr: 0.813 ± 0.264
0.0IleXaa: 0.0 ± 0.0
Lys
4.627LysAla: 4.627 ± 0.704
0.188LysCys: 0.188 ± 0.114
3.189LysAsp: 3.189 ± 0.409
2.626LysGlu: 2.626 ± 0.441
1.251LysPhe: 1.251 ± 0.257
3.439LysGly: 3.439 ± 0.379
1.001LysHis: 1.001 ± 0.237
1.876LysIle: 1.876 ± 0.271
2.751LysLys: 2.751 ± 0.475
3.252LysLeu: 3.252 ± 0.451
1.001LysMet: 1.001 ± 0.209
1.876LysAsn: 1.876 ± 0.428
2.939LysPro: 2.939 ± 0.576
1.876LysGln: 1.876 ± 0.286
3.377LysArg: 3.377 ± 0.517
2.626LysSer: 2.626 ± 0.447
2.126LysThr: 2.126 ± 0.348
4.69LysVal: 4.69 ± 0.576
0.813LysTrp: 0.813 ± 0.208
1.126LysTyr: 1.126 ± 0.328
0.0LysXaa: 0.0 ± 0.0
Leu
7.816LeuAla: 7.816 ± 0.705
0.688LeuCys: 0.688 ± 0.241
4.94LeuAsp: 4.94 ± 0.446
6.628LeuGlu: 6.628 ± 0.7
2.439LeuPhe: 2.439 ± 0.388
5.44LeuGly: 5.44 ± 0.556
2.564LeuHis: 2.564 ± 0.471
3.939LeuIle: 3.939 ± 0.518
4.377LeuLys: 4.377 ± 0.756
4.94LeuLeu: 4.94 ± 0.725
2.251LeuMet: 2.251 ± 0.361
2.814LeuAsn: 2.814 ± 0.44
4.315LeuPro: 4.315 ± 0.534
2.376LeuGln: 2.376 ± 0.415
5.503LeuArg: 5.503 ± 0.59
5.253LeuSer: 5.253 ± 0.745
4.877LeuThr: 4.877 ± 0.515
5.253LeuVal: 5.253 ± 0.584
2.001LeuTrp: 2.001 ± 0.319
2.876LeuTyr: 2.876 ± 0.412
0.0LeuXaa: 0.0 ± 0.0
Met
2.251MetAla: 2.251 ± 0.323
0.125MetCys: 0.125 ± 0.098
0.813MetAsp: 0.813 ± 0.217
1.376MetGlu: 1.376 ± 0.24
0.625MetPhe: 0.625 ± 0.177
1.501MetGly: 1.501 ± 0.317
0.438MetHis: 0.438 ± 0.153
1.501MetIle: 1.501 ± 0.279
1.938MetLys: 1.938 ± 0.341
2.251MetLeu: 2.251 ± 0.373
0.375MetMet: 0.375 ± 0.161
0.625MetAsn: 0.625 ± 0.188
1.251MetPro: 1.251 ± 0.336
0.688MetGln: 0.688 ± 0.2
1.313MetArg: 1.313 ± 0.271
2.501MetSer: 2.501 ± 0.404
2.126MetThr: 2.126 ± 0.412
1.251MetVal: 1.251 ± 0.298
0.375MetTrp: 0.375 ± 0.16
0.563MetTyr: 0.563 ± 0.204
0.0MetXaa: 0.0 ± 0.0
Asn
2.814AsnAla: 2.814 ± 0.488
0.375AsnCys: 0.375 ± 0.153
1.751AsnAsp: 1.751 ± 0.284
2.126AsnGlu: 2.126 ± 0.469
1.251AsnPhe: 1.251 ± 0.29
3.377AsnGly: 3.377 ± 0.447
1.001AsnHis: 1.001 ± 0.213
1.813AsnIle: 1.813 ± 0.323
1.001AsnLys: 1.001 ± 0.237
3.314AsnLeu: 3.314 ± 0.398
0.5AsnMet: 0.5 ± 0.152
0.875AsnAsn: 0.875 ± 0.255
2.501AsnPro: 2.501 ± 0.432
1.126AsnGln: 1.126 ± 0.257
1.876AsnArg: 1.876 ± 0.304
1.876AsnSer: 1.876 ± 0.381
2.126AsnThr: 2.126 ± 0.396
2.564AsnVal: 2.564 ± 0.357
0.813AsnTrp: 0.813 ± 0.229
1.001AsnTyr: 1.001 ± 0.208
0.0AsnXaa: 0.0 ± 0.0
Pro
5.253ProAla: 5.253 ± 0.479
0.563ProCys: 0.563 ± 0.198
4.315ProAsp: 4.315 ± 0.474
4.565ProGlu: 4.565 ± 0.482
1.813ProPhe: 1.813 ± 0.347
3.752ProGly: 3.752 ± 0.517
0.875ProHis: 0.875 ± 0.25
2.064ProIle: 2.064 ± 0.352
3.064ProLys: 3.064 ± 0.496
4.127ProLeu: 4.127 ± 0.611
1.188ProMet: 1.188 ± 0.287
2.689ProAsn: 2.689 ± 0.445
2.251ProPro: 2.251 ± 0.392
2.314ProGln: 2.314 ± 0.482
3.127ProArg: 3.127 ± 0.436
2.564ProSer: 2.564 ± 0.409
3.689ProThr: 3.689 ± 0.492
3.752ProVal: 3.752 ± 0.465
1.251ProTrp: 1.251 ± 0.39
1.438ProTyr: 1.438 ± 0.277
0.0ProXaa: 0.0 ± 0.0
Gln
4.19GlnAla: 4.19 ± 0.572
0.188GlnCys: 0.188 ± 0.117
1.938GlnAsp: 1.938 ± 0.358
1.938GlnGlu: 1.938 ± 0.389
1.251GlnPhe: 1.251 ± 0.232
3.627GlnGly: 3.627 ± 0.47
0.563GlnHis: 0.563 ± 0.156
2.626GlnIle: 2.626 ± 0.374
1.563GlnLys: 1.563 ± 0.302
3.502GlnLeu: 3.502 ± 0.516
0.875GlnMet: 0.875 ± 0.208
0.5GlnAsn: 0.5 ± 0.196
1.563GlnPro: 1.563 ± 0.358
1.188GlnGln: 1.188 ± 0.396
2.439GlnArg: 2.439 ± 0.377
1.501GlnSer: 1.501 ± 0.284
1.751GlnThr: 1.751 ± 0.292
2.251GlnVal: 2.251 ± 0.375
0.813GlnTrp: 0.813 ± 0.278
1.313GlnTyr: 1.313 ± 0.231
0.0GlnXaa: 0.0 ± 0.0
Arg
6.691ArgAla: 6.691 ± 0.74
0.813ArgCys: 0.813 ± 0.331
4.315ArgAsp: 4.315 ± 0.577
4.252ArgGlu: 4.252 ± 0.568
2.314ArgPhe: 2.314 ± 0.405
4.002ArgGly: 4.002 ± 0.495
1.313ArgHis: 1.313 ± 0.289
3.689ArgIle: 3.689 ± 0.509
3.064ArgLys: 3.064 ± 0.579
5.44ArgLeu: 5.44 ± 0.506
1.813ArgMet: 1.813 ± 0.314
1.688ArgAsn: 1.688 ± 0.365
3.127ArgPro: 3.127 ± 0.43
2.251ArgGln: 2.251 ± 0.376
6.316ArgArg: 6.316 ± 0.77
2.689ArgSer: 2.689 ± 0.388
2.501ArgThr: 2.501 ± 0.357
5.065ArgVal: 5.065 ± 0.561
1.501ArgTrp: 1.501 ± 0.329
2.001ArgTyr: 2.001 ± 0.351
0.0ArgXaa: 0.0 ± 0.0
Ser
4.94SerAla: 4.94 ± 0.577
0.5SerCys: 0.5 ± 0.18
3.002SerAsp: 3.002 ± 0.43
3.439SerGlu: 3.439 ± 0.533
1.688SerPhe: 1.688 ± 0.333
4.44SerGly: 4.44 ± 0.571
0.75SerHis: 0.75 ± 0.204
2.251SerIle: 2.251 ± 0.349
2.001SerLys: 2.001 ± 0.465
4.065SerLeu: 4.065 ± 0.494
1.501SerMet: 1.501 ± 0.232
1.688SerAsn: 1.688 ± 0.363
3.377SerPro: 3.377 ± 0.497
2.251SerGln: 2.251 ± 0.441
3.502SerArg: 3.502 ± 0.532
2.564SerSer: 2.564 ± 0.506
2.689SerThr: 2.689 ± 0.409
3.939SerVal: 3.939 ± 0.447
1.251SerTrp: 1.251 ± 0.25
1.438SerTyr: 1.438 ± 0.267
0.0SerXaa: 0.0 ± 0.0
Thr
5.628ThrAla: 5.628 ± 0.5
0.438ThrCys: 0.438 ± 0.144
4.565ThrAsp: 4.565 ± 0.608
3.127ThrGlu: 3.127 ± 0.447
2.001ThrPhe: 2.001 ± 0.329
5.315ThrGly: 5.315 ± 0.605
0.875ThrHis: 0.875 ± 0.259
2.064ThrIle: 2.064 ± 0.349
2.501ThrLys: 2.501 ± 0.416
4.502ThrLeu: 4.502 ± 0.528
1.313ThrMet: 1.313 ± 0.316
1.813ThrAsn: 1.813 ± 0.283
4.065ThrPro: 4.065 ± 0.554
2.189ThrGln: 2.189 ± 0.431
2.626ThrArg: 2.626 ± 0.357
3.189ThrSer: 3.189 ± 0.561
3.627ThrThr: 3.627 ± 0.515
4.502ThrVal: 4.502 ± 0.529
0.563ThrTrp: 0.563 ± 0.15
2.064ThrTyr: 2.064 ± 0.39
0.0ThrXaa: 0.0 ± 0.0
Val
7.441ValAla: 7.441 ± 0.641
0.625ValCys: 0.625 ± 0.173
5.065ValAsp: 5.065 ± 0.604
5.253ValGlu: 5.253 ± 0.654
2.626ValPhe: 2.626 ± 0.382
4.565ValGly: 4.565 ± 0.46
1.063ValHis: 1.063 ± 0.256
3.877ValIle: 3.877 ± 0.43
3.689ValLys: 3.689 ± 0.449
5.565ValLeu: 5.565 ± 0.684
1.563ValMet: 1.563 ± 0.344
3.314ValAsn: 3.314 ± 0.487
3.502ValPro: 3.502 ± 0.484
2.814ValGln: 2.814 ± 0.353
4.19ValArg: 4.19 ± 0.573
3.627ValSer: 3.627 ± 0.486
4.877ValThr: 4.877 ± 0.621
5.878ValVal: 5.878 ± 0.748
1.501ValTrp: 1.501 ± 0.307
2.501ValTyr: 2.501 ± 0.507
0.0ValXaa: 0.0 ± 0.0
Trp
2.064TrpAla: 2.064 ± 0.414
0.375TrpCys: 0.375 ± 0.164
1.563TrpAsp: 1.563 ± 0.232
1.376TrpGlu: 1.376 ± 0.264
0.875TrpPhe: 0.875 ± 0.289
1.251TrpGly: 1.251 ± 0.278
0.563TrpHis: 0.563 ± 0.189
1.001TrpIle: 1.001 ± 0.248
0.75TrpLys: 0.75 ± 0.203
1.376TrpLeu: 1.376 ± 0.32
0.5TrpMet: 0.5 ± 0.189
0.625TrpAsn: 0.625 ± 0.233
0.938TrpPro: 0.938 ± 0.265
1.001TrpGln: 1.001 ± 0.207
1.188TrpArg: 1.188 ± 0.234
1.126TrpSer: 1.126 ± 0.25
1.126TrpThr: 1.126 ± 0.251
1.438TrpVal: 1.438 ± 0.271
0.438TrpTrp: 0.438 ± 0.186
0.625TrpTyr: 0.625 ± 0.201
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.814TyrAla: 2.814 ± 0.369
0.313TyrCys: 0.313 ± 0.144
2.376TyrAsp: 2.376 ± 0.338
1.813TyrGlu: 1.813 ± 0.379
0.75TyrPhe: 0.75 ± 0.266
2.126TyrGly: 2.126 ± 0.339
0.438TyrHis: 0.438 ± 0.167
1.876TyrIle: 1.876 ± 0.333
1.188TyrLys: 1.188 ± 0.272
2.814TyrLeu: 2.814 ± 0.416
0.438TyrMet: 0.438 ± 0.175
0.938TyrAsn: 0.938 ± 0.251
1.313TyrPro: 1.313 ± 0.258
1.251TyrGln: 1.251 ± 0.281
1.938TyrArg: 1.938 ± 0.347
1.501TyrSer: 1.501 ± 0.356
1.626TyrThr: 1.626 ± 0.358
2.689TyrVal: 2.689 ± 0.416
0.438TyrTrp: 0.438 ± 0.164
0.938TyrTyr: 0.938 ± 0.301
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (15993 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski