Phormidium ambiguum IAM M-71

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Oscillatoriaceae; Phormidium; Phormidium ambiguum

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7I3K8|A0A1U7I3K8_9CYAN Uncharacterized protein OS=Phormidium ambiguum IAM M-71 OX=454136 GN=NIES2119_30420 PE=4 SV=1
MM1 pKa = 7.16TVNIYY6 pKa = 11.22SNVPGDD12 pKa = 3.71GFASEE17 pKa = 4.12EE18 pKa = 4.15TKK20 pKa = 10.69LYY22 pKa = 11.12NLVNEE27 pKa = 4.19YY28 pKa = 10.22RR29 pKa = 11.84AQNGLAPITASKK41 pKa = 10.77ALSTVANRR49 pKa = 11.84HH50 pKa = 4.81VLDD53 pKa = 3.78LAEE56 pKa = 4.91NIGTLTHH63 pKa = 6.01SWSDD67 pKa = 3.41APYY70 pKa = 10.51DD71 pKa = 3.75PSNSSTYY78 pKa = 10.53SAMWSAPQRR87 pKa = 11.84FNTGYY92 pKa = 10.55VGNGYY97 pKa = 10.31EE98 pKa = 4.1NAFGGSGSYY107 pKa = 9.46IANATDD113 pKa = 5.53ALQAWKK119 pKa = 10.67NSPAHH124 pKa = 5.86NAVILNQGMWQNLQWNALGVGLYY147 pKa = 9.99KK148 pKa = 10.71GYY150 pKa = 11.05AVLWFGQQPDD160 pKa = 3.82TTGTPEE166 pKa = 5.75LITTSSSTSDD176 pKa = 3.36PNTGNNSTSVLASTTPTNSPIFGTEE201 pKa = 3.97GNDD204 pKa = 3.35TLLGTEE210 pKa = 5.01GDD212 pKa = 4.04DD213 pKa = 3.76TLHH216 pKa = 6.75GLGGNDD222 pKa = 3.38LFYY225 pKa = 11.16AGGGNDD231 pKa = 3.16ILFGNLGSDD240 pKa = 3.69SLNGNLGNDD249 pKa = 3.34SLYY252 pKa = 11.0GGKK255 pKa = 9.92DD256 pKa = 3.13ADD258 pKa = 4.1ILWGGKK264 pKa = 10.29GEE266 pKa = 4.12DD267 pKa = 3.47ALYY270 pKa = 11.19GNLGNDD276 pKa = 3.71LLYY279 pKa = 11.33GNIGQDD285 pKa = 2.53ILYY288 pKa = 10.05GGRR291 pKa = 11.84EE292 pKa = 3.95ADD294 pKa = 3.47TLYY297 pKa = 10.97GGQDD301 pKa = 3.57DD302 pKa = 5.32DD303 pKa = 5.27VLFGDD308 pKa = 5.1LDD310 pKa = 3.92NDD312 pKa = 3.61ILFGDD317 pKa = 4.56FGVDD321 pKa = 2.98TLTGGEE327 pKa = 4.11GSDD330 pKa = 3.87IFGLQLDD337 pKa = 4.19RR338 pKa = 11.84GTDD341 pKa = 3.19IVSDD345 pKa = 3.78FTDD348 pKa = 4.09GVDD351 pKa = 4.61FLGLSAGLSFANLSITQGTGTDD373 pKa = 3.38AANTLISANGQLLAVLQGVQAITITEE399 pKa = 3.75ADD401 pKa = 3.17IRR403 pKa = 11.84LLL405 pKa = 3.89

Molecular weight: 42.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7IJJ4|A0A1U7IJJ4_9CYAN RND transporter OS=Phormidium ambiguum IAM M-71 OX=454136 GN=NIES2119_14070 PE=3 SV=1
MM1 pKa = 6.63TQRR4 pKa = 11.84TLHH7 pKa = 4.82GTSRR11 pKa = 11.84KK12 pKa = 9.46RR13 pKa = 11.84KK14 pKa = 7.05RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 10.45NGKK29 pKa = 9.01LVLKK33 pKa = 10.49ARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.34KK38 pKa = 10.23GRR40 pKa = 11.84YY41 pKa = 8.55RR42 pKa = 11.84LAVV45 pKa = 3.33

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6148

0

6148

2062752

29

3377

335.5

37.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.855 ± 0.034

0.963 ± 0.011

4.74 ± 0.029

6.588 ± 0.033

4.061 ± 0.02

6.528 ± 0.039

1.664 ± 0.015

7.126 ± 0.026

5.185 ± 0.029

10.924 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.752 ± 0.014

4.825 ± 0.037

4.614 ± 0.024

5.3 ± 0.027

4.996 ± 0.024

6.365 ± 0.031

5.684 ± 0.027

6.362 ± 0.026

1.449 ± 0.014

3.018 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski