Pseudomonas phage phiNN

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Vidaverviricetes; Mindivirales; Cystoviridae; Cystovirus; Pseudomonas virus phiNN

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4N5V3|A0A0B4N5V3_9VIRU Uncharacterized protein OS=Pseudomonas phage phiNN OX=1603039 PE=4 SV=1
MM1 pKa = 7.39TLYY4 pKa = 10.67LVPPLDD10 pKa = 4.0SADD13 pKa = 3.84KK14 pKa = 10.13EE15 pKa = 4.4LPALASKK22 pKa = 10.9AGVEE26 pKa = 3.92LLEE29 pKa = 4.29VEE31 pKa = 4.92FLHH34 pKa = 6.56EE35 pKa = 4.62LWPHH39 pKa = 6.23LSGGQIVIAAMSANNLAILNRR60 pKa = 11.84YY61 pKa = 7.73MSTLLKK67 pKa = 9.16EE68 pKa = 4.22LPVAVMAVPGASYY81 pKa = 10.62RR82 pKa = 11.84SDD84 pKa = 2.83WNMIAHH90 pKa = 7.69ALPSEE95 pKa = 4.09DD96 pKa = 4.43WITLSSKK103 pKa = 8.0MLKK106 pKa = 10.26SGLLANDD113 pKa = 3.86TVQGQKK119 pKa = 10.45RR120 pKa = 11.84SGAEE124 pKa = 3.4PLAPNTYY131 pKa = 8.55TAALSRR137 pKa = 11.84LGIGTPDD144 pKa = 3.38AKK146 pKa = 10.72VDD148 pKa = 3.78EE149 pKa = 4.98PEE151 pKa = 3.93QPFDD155 pKa = 3.83VDD157 pKa = 3.5EE158 pKa = 4.49VTAA161 pKa = 4.62

Molecular weight: 17.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4N5K7|A0A0B4N5K7_9VIRU Uncharacterized protein OS=Pseudomonas phage phiNN OX=1603039 PE=4 SV=1
MM1 pKa = 7.39AVVNVNVTQLDD12 pKa = 3.76SALRR16 pKa = 11.84PLLQLARR23 pKa = 11.84THH25 pKa = 6.84SDD27 pKa = 2.87ANLVVYY33 pKa = 10.6ARR35 pKa = 11.84GLTPSQRR42 pKa = 11.84TALYY46 pKa = 10.55KK47 pKa = 10.5LLKK50 pKa = 10.46DD51 pKa = 3.78LDD53 pKa = 4.04VVIPRR58 pKa = 11.84FNKK61 pKa = 10.17ALVSATTLVARR72 pKa = 11.84GLL74 pKa = 3.72

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

3617

56

769

278.2

30.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.948 ± 0.534

0.636 ± 0.15

5.502 ± 0.432

4.894 ± 0.403

3.843 ± 0.317

7.575 ± 0.682

1.548 ± 0.304

5.281 ± 0.481

4.589 ± 0.331

10.064 ± 0.426

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.23

3.566 ± 0.27

4.866 ± 0.277

3.179 ± 0.268

5.115 ± 0.52

7.409 ± 0.233

6.11 ± 0.473

7.99 ± 0.821

1.327 ± 0.233

2.875 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski