Amino acid dipepetide frequency for Rhodovulum sp. 12E13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.667AlaAla: 23.667 ± 0.275
1.295AlaCys: 1.295 ± 0.035
7.82AlaAsp: 7.82 ± 0.105
10.726AlaGlu: 10.726 ± 0.11
4.63AlaPhe: 4.63 ± 0.073
13.737AlaGly: 13.737 ± 0.13
2.784AlaHis: 2.784 ± 0.056
5.477AlaIle: 5.477 ± 0.073
2.338AlaLys: 2.338 ± 0.056
16.961AlaLeu: 16.961 ± 0.194
3.879AlaMet: 3.879 ± 0.061
2.085AlaAsn: 2.085 ± 0.044
8.389AlaPro: 8.389 ± 0.126
4.217AlaGln: 4.217 ± 0.059
12.844AlaArg: 12.844 ± 0.137
5.322AlaSer: 5.322 ± 0.075
6.742AlaThr: 6.742 ± 0.074
9.796AlaVal: 9.796 ± 0.103
1.841AlaTrp: 1.841 ± 0.039
2.467AlaTyr: 2.467 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
1.163CysAla: 1.163 ± 0.035
0.102CysCys: 0.102 ± 0.009
0.513CysAsp: 0.513 ± 0.019
0.435CysGlu: 0.435 ± 0.019
0.274CysPhe: 0.274 ± 0.013
0.929CysGly: 0.929 ± 0.028
0.251CysHis: 0.251 ± 0.013
0.32CysIle: 0.32 ± 0.015
0.134CysLys: 0.134 ± 0.01
0.822CysLeu: 0.822 ± 0.027
0.144CysMet: 0.144 ± 0.01
0.164CysAsn: 0.164 ± 0.011
0.573CysPro: 0.573 ± 0.023
0.172CysGln: 0.172 ± 0.011
0.666CysArg: 0.666 ± 0.021
0.359CysSer: 0.359 ± 0.017
0.421CysThr: 0.421 ± 0.017
0.566CysVal: 0.566 ± 0.02
0.116CysTrp: 0.116 ± 0.009
0.164CysTyr: 0.164 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
8.123AspAla: 8.123 ± 0.087
0.431AspCys: 0.431 ± 0.021
3.55AspAsp: 3.55 ± 0.074
3.756AspGlu: 3.756 ± 0.058
1.961AspPhe: 1.961 ± 0.042
5.599AspGly: 5.599 ± 0.081
1.189AspHis: 1.189 ± 0.032
2.556AspIle: 2.556 ± 0.045
1.027AspLys: 1.027 ± 0.032
6.2AspLeu: 6.2 ± 0.08
1.373AspMet: 1.373 ± 0.035
0.957AspAsn: 0.957 ± 0.032
4.006AspPro: 4.006 ± 0.064
1.291AspGln: 1.291 ± 0.035
5.113AspArg: 5.113 ± 0.073
1.853AspSer: 1.853 ± 0.038
2.828AspThr: 2.828 ± 0.051
4.06AspVal: 4.06 ± 0.061
1.181AspTrp: 1.181 ± 0.035
1.352AspTyr: 1.352 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
10.553GluAla: 10.553 ± 0.124
0.336GluCys: 0.336 ± 0.016
2.942GluAsp: 2.942 ± 0.047
3.72GluGlu: 3.72 ± 0.066
1.593GluPhe: 1.593 ± 0.033
6.223GluGly: 6.223 ± 0.092
1.167GluHis: 1.167 ± 0.032
3.509GluIle: 3.509 ± 0.057
1.446GluLys: 1.446 ± 0.045
4.897GluLeu: 4.897 ± 0.072
1.806GluMet: 1.806 ± 0.036
1.215GluAsn: 1.215 ± 0.032
3.022GluPro: 3.022 ± 0.063
1.777GluGln: 1.777 ± 0.038
5.862GluArg: 5.862 ± 0.072
2.279GluSer: 2.279 ± 0.047
4.142GluThr: 4.142 ± 0.059
4.747GluVal: 4.747 ± 0.068
0.76GluTrp: 0.76 ± 0.027
0.866GluTyr: 0.866 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
4.639PheAla: 4.639 ± 0.06
0.351PheCys: 0.351 ± 0.017
2.565PheAsp: 2.565 ± 0.052
2.083PheGlu: 2.083 ± 0.043
1.227PhePhe: 1.227 ± 0.037
3.562PheGly: 3.562 ± 0.061
0.77PheHis: 0.77 ± 0.023
1.137PheIle: 1.137 ± 0.037
0.509PheLys: 0.509 ± 0.018
3.345PheLeu: 3.345 ± 0.053
0.671PheMet: 0.671 ± 0.021
0.72PheAsn: 0.72 ± 0.028
1.534PhePro: 1.534 ± 0.037
0.782PheGln: 0.782 ± 0.023
2.353PheArg: 2.353 ± 0.047
1.686PheSer: 1.686 ± 0.039
1.783PheThr: 1.783 ± 0.039
2.712PheVal: 2.712 ± 0.054
0.531PheTrp: 0.531 ± 0.023
0.765PheTyr: 0.765 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
13.052GlyAla: 13.052 ± 0.129
0.845GlyCys: 0.845 ± 0.03
5.005GlyAsp: 5.005 ± 0.083
5.54GlyGlu: 5.54 ± 0.073
3.373GlyPhe: 3.373 ± 0.063
8.752GlyGly: 8.752 ± 0.116
2.123GlyHis: 2.123 ± 0.042
3.94GlyIle: 3.94 ± 0.058
2.145GlyLys: 2.145 ± 0.058
10.017GlyLeu: 10.017 ± 0.106
2.55GlyMet: 2.55 ± 0.045
1.621GlyAsn: 1.621 ± 0.044
4.859GlyPro: 4.859 ± 0.067
2.818GlyGln: 2.818 ± 0.051
7.924GlyArg: 7.924 ± 0.082
3.807GlySer: 3.807 ± 0.066
5.037GlyThr: 5.037 ± 0.073
6.649GlyVal: 6.649 ± 0.073
1.734GlyTrp: 1.734 ± 0.043
2.11GlyTyr: 2.11 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
2.787HisAla: 2.787 ± 0.06
0.217HisCys: 0.217 ± 0.011
1.393HisAsp: 1.393 ± 0.036
1.183HisGlu: 1.183 ± 0.029
0.726HisPhe: 0.726 ± 0.024
2.065HisGly: 2.065 ± 0.042
0.502HisHis: 0.502 ± 0.024
0.664HisIle: 0.664 ± 0.023
0.318HisLys: 0.318 ± 0.013
2.116HisLeu: 2.116 ± 0.045
0.449HisMet: 0.449 ± 0.019
0.329HisAsn: 0.329 ± 0.016
1.425HisPro: 1.425 ± 0.041
0.441HisGln: 0.441 ± 0.017
1.605HisArg: 1.605 ± 0.044
0.728HisSer: 0.728 ± 0.023
0.793HisThr: 0.793 ± 0.028
1.69HisVal: 1.69 ± 0.032
0.339HisTrp: 0.339 ± 0.017
0.535HisTyr: 0.535 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
6.568IleAla: 6.568 ± 0.073
0.426IleCys: 0.426 ± 0.02
3.18IleAsp: 3.18 ± 0.052
3.087IleGlu: 3.087 ± 0.055
1.361IlePhe: 1.361 ± 0.033
4.264IleGly: 4.264 ± 0.061
0.757IleHis: 0.757 ± 0.025
1.137IleIle: 1.137 ± 0.036
0.644IleLys: 0.644 ± 0.026
3.94IleLeu: 3.94 ± 0.065
0.741IleMet: 0.741 ± 0.026
0.848IleAsn: 0.848 ± 0.027
2.067IlePro: 2.067 ± 0.044
0.794IleGln: 0.794 ± 0.027
3.073IleArg: 3.073 ± 0.052
2.006IleSer: 2.006 ± 0.043
2.01IleThr: 2.01 ± 0.04
3.74IleVal: 3.74 ± 0.069
0.566IleTrp: 0.566 ± 0.021
0.855IleTyr: 0.855 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
2.677LysAla: 2.677 ± 0.047
0.123LysCys: 0.123 ± 0.01
1.003LysAsp: 1.003 ± 0.031
0.996LysGlu: 0.996 ± 0.032
0.499LysPhe: 0.499 ± 0.019
1.886LysGly: 1.886 ± 0.047
0.375LysHis: 0.375 ± 0.018
0.949LysIle: 0.949 ± 0.03
0.668LysLys: 0.668 ± 0.025
1.761LysLeu: 1.761 ± 0.046
0.493LysMet: 0.493 ± 0.022
0.419LysAsn: 0.419 ± 0.019
1.174LysPro: 1.174 ± 0.032
0.526LysGln: 0.526 ± 0.024
1.709LysArg: 1.709 ± 0.04
1.023LysSer: 1.023 ± 0.03
1.222LysThr: 1.222 ± 0.036
1.545LysVal: 1.545 ± 0.038
0.227LysTrp: 0.227 ± 0.014
0.385LysTyr: 0.385 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
16.352LeuAla: 16.352 ± 0.187
0.897LeuCys: 0.897 ± 0.025
6.201LeuAsp: 6.201 ± 0.076
5.592LeuGlu: 5.592 ± 0.071
3.425LeuPhe: 3.425 ± 0.065
8.898LeuGly: 8.898 ± 0.101
1.975LeuHis: 1.975 ± 0.043
4.241LeuIle: 4.241 ± 0.067
2.112LeuLys: 2.112 ± 0.049
8.94LeuLeu: 8.94 ± 0.129
2.356LeuMet: 2.356 ± 0.05
1.824LeuAsn: 1.824 ± 0.042
6.083LeuPro: 6.083 ± 0.069
2.27LeuGln: 2.27 ± 0.044
7.911LeuArg: 7.911 ± 0.098
5.926LeuSer: 5.926 ± 0.078
5.542LeuThr: 5.542 ± 0.058
7.778LeuVal: 7.778 ± 0.087
1.429LeuTrp: 1.429 ± 0.037
1.824LeuTyr: 1.824 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
3.454MetAla: 3.454 ± 0.055
0.168MetCys: 0.168 ± 0.009
1.199MetAsp: 1.199 ± 0.03
1.363MetGlu: 1.363 ± 0.035
0.665MetPhe: 0.665 ± 0.025
2.103MetGly: 2.103 ± 0.043
0.446MetHis: 0.446 ± 0.019
1.171MetIle: 1.171 ± 0.031
0.678MetLys: 0.678 ± 0.022
2.26MetLeu: 2.26 ± 0.047
0.576MetMet: 0.576 ± 0.023
0.595MetAsn: 0.595 ± 0.022
1.538MetPro: 1.538 ± 0.038
0.758MetGln: 0.758 ± 0.027
1.931MetArg: 1.931 ± 0.037
1.411MetSer: 1.411 ± 0.029
1.797MetThr: 1.797 ± 0.037
1.689MetVal: 1.689 ± 0.039
0.234MetTrp: 0.234 ± 0.016
0.26MetTyr: 0.26 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
2.524AsnAla: 2.524 ± 0.047
0.161AsnCys: 0.161 ± 0.012
0.967AsnAsp: 0.967 ± 0.035
0.881AsnGlu: 0.881 ± 0.023
0.694AsnPhe: 0.694 ± 0.025
1.649AsnGly: 1.649 ± 0.043
0.348AsnHis: 0.348 ± 0.016
0.892AsnIle: 0.892 ± 0.028
0.351AsnLys: 0.351 ± 0.017
1.822AsnLeu: 1.822 ± 0.04
0.445AsnMet: 0.445 ± 0.02
0.41AsnAsn: 0.41 ± 0.02
1.372AsnPro: 1.372 ± 0.035
0.443AsnGln: 0.443 ± 0.02
1.449AsnArg: 1.449 ± 0.034
0.724AsnSer: 0.724 ± 0.026
0.899AsnThr: 0.899 ± 0.028
1.548AsnVal: 1.548 ± 0.042
0.305AsnTrp: 0.305 ± 0.016
0.462AsnTyr: 0.462 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
8.073ProAla: 8.073 ± 0.116
0.417ProCys: 0.417 ± 0.017
4.341ProAsp: 4.341 ± 0.062
4.729ProGlu: 4.729 ± 0.077
2.041ProPhe: 2.041 ± 0.04
6.195ProGly: 6.195 ± 0.092
1.193ProHis: 1.193 ± 0.03
1.91ProIle: 1.91 ± 0.04
1.03ProLys: 1.03 ± 0.033
5.252ProLeu: 5.252 ± 0.079
1.2ProMet: 1.2 ± 0.03
0.946ProAsn: 0.946 ± 0.031
3.53ProPro: 3.53 ± 0.074
1.552ProGln: 1.552 ± 0.041
4.078ProArg: 4.078 ± 0.068
2.463ProSer: 2.463 ± 0.044
2.394ProThr: 2.394 ± 0.047
4.697ProVal: 4.697 ± 0.069
0.823ProTrp: 0.823 ± 0.028
1.118ProTyr: 1.118 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
4.018GlnAla: 4.018 ± 0.058
0.16GlnCys: 0.16 ± 0.013
1.307GlnAsp: 1.307 ± 0.033
1.537GlnGlu: 1.537 ± 0.041
0.773GlnPhe: 0.773 ± 0.03
2.448GlnGly: 2.448 ± 0.047
0.433GlnHis: 0.433 ± 0.018
1.47GlnIle: 1.47 ± 0.033
0.64GlnLys: 0.64 ± 0.027
2.156GlnLeu: 2.156 ± 0.044
0.749GlnMet: 0.749 ± 0.022
0.566GlnAsn: 0.566 ± 0.026
1.544GlnPro: 1.544 ± 0.036
0.804GlnGln: 0.804 ± 0.033
1.967GlnArg: 1.967 ± 0.04
1.247GlnSer: 1.247 ± 0.034
1.561GlnThr: 1.561 ± 0.037
2.166GlnVal: 2.166 ± 0.041
0.306GlnTrp: 0.306 ± 0.015
0.412GlnTyr: 0.412 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
11.839ArgAla: 11.839 ± 0.137
0.566ArgCys: 0.566 ± 0.022
4.716ArgAsp: 4.716 ± 0.064
4.684ArgGlu: 4.684 ± 0.071
2.846ArgPhe: 2.846 ± 0.05
6.001ArgGly: 6.001 ± 0.076
1.887ArgHis: 1.887 ± 0.042
3.929ArgIle: 3.929 ± 0.056
1.705ArgLys: 1.705 ± 0.04
9.515ArgLeu: 9.515 ± 0.099
2.178ArgMet: 2.178 ± 0.041
1.46ArgAsn: 1.46 ± 0.034
4.847ArgPro: 4.847 ± 0.071
2.358ArgGln: 2.358 ± 0.052
7.142ArgArg: 7.142 ± 0.101
3.272ArgSer: 3.272 ± 0.052
3.53ArgThr: 3.53 ± 0.057
5.668ArgVal: 5.668 ± 0.072
1.132ArgTrp: 1.132 ± 0.03
1.551ArgTyr: 1.551 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
5.697SerAla: 5.697 ± 0.076
0.362SerCys: 0.362 ± 0.018
2.653SerAsp: 2.653 ± 0.049
2.461SerGlu: 2.461 ± 0.047
1.769SerPhe: 1.769 ± 0.037
5.076SerGly: 5.076 ± 0.077
0.866SerHis: 0.866 ± 0.027
1.803SerIle: 1.803 ± 0.04
0.827SerLys: 0.827 ± 0.029
4.371SerLeu: 4.371 ± 0.06
1.061SerMet: 1.061 ± 0.031
0.865SerAsn: 0.865 ± 0.025
2.572SerPro: 2.572 ± 0.047
1.097SerGln: 1.097 ± 0.032
3.291SerArg: 3.291 ± 0.056
1.937SerSer: 1.937 ± 0.044
2.082SerThr: 2.082 ± 0.044
3.342SerVal: 3.342 ± 0.052
0.594SerTrp: 0.594 ± 0.022
1.01SerTyr: 1.01 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
6.586ThrAla: 6.586 ± 0.07
0.444ThrCys: 0.444 ± 0.019
2.879ThrAsp: 2.879 ± 0.05
3.058ThrGlu: 3.058 ± 0.05
1.862ThrPhe: 1.862 ± 0.039
5.771ThrGly: 5.771 ± 0.077
1.037ThrHis: 1.037 ± 0.033
2.175ThrIle: 2.175 ± 0.047
0.89ThrLys: 0.89 ± 0.03
5.963ThrLeu: 5.963 ± 0.063
1.059ThrMet: 1.059 ± 0.029
0.934ThrAsn: 0.934 ± 0.027
3.379ThrPro: 3.379 ± 0.05
1.229ThrGln: 1.229 ± 0.032
3.728ThrArg: 3.728 ± 0.059
2.209ThrSer: 2.209 ± 0.039
2.582ThrThr: 2.582 ± 0.051
4.35ThrVal: 4.35 ± 0.065
0.73ThrTrp: 0.73 ± 0.023
1.084ThrTyr: 1.084 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
10.909ValAla: 10.909 ± 0.097
0.64ValCys: 0.64 ± 0.021
4.128ValAsp: 4.128 ± 0.065
5.047ValGlu: 5.047 ± 0.076
2.737ValPhe: 2.737 ± 0.045
5.751ValGly: 5.751 ± 0.068
1.408ValHis: 1.408 ± 0.036
3.52ValIle: 3.52 ± 0.063
1.544ValLys: 1.544 ± 0.044
7.497ValLeu: 7.497 ± 0.091
1.811ValMet: 1.811 ± 0.034
1.612ValAsn: 1.612 ± 0.041
4.25ValPro: 4.25 ± 0.06
1.85ValGln: 1.85 ± 0.036
5.215ValArg: 5.215 ± 0.062
3.852ValSer: 3.852 ± 0.059
4.835ValThr: 4.835 ± 0.066
6.023ValVal: 6.023 ± 0.074
1.013ValTrp: 1.013 ± 0.031
1.386ValTyr: 1.386 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.746TrpAla: 1.746 ± 0.043
0.138TrpCys: 0.138 ± 0.011
0.722TrpAsp: 0.722 ± 0.028
0.811TrpGlu: 0.811 ± 0.024
0.537TrpPhe: 0.537 ± 0.021
1.101TrpGly: 1.101 ± 0.034
0.349TrpHis: 0.349 ± 0.018
0.602TrpIle: 0.602 ± 0.023
0.318TrpLys: 0.318 ± 0.015
1.705TrpLeu: 1.705 ± 0.045
0.368TrpMet: 0.368 ± 0.018
0.335TrpAsn: 0.335 ± 0.018
0.846TrpPro: 0.846 ± 0.028
0.585TrpGln: 0.585 ± 0.019
1.393TrpArg: 1.393 ± 0.038
0.771TrpSer: 0.771 ± 0.026
0.739TrpThr: 0.739 ± 0.029
0.892TrpVal: 0.892 ± 0.028
0.269TrpTrp: 0.269 ± 0.017
0.248TrpTyr: 0.248 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.589TyrAla: 2.589 ± 0.051
0.207TyrCys: 0.207 ± 0.012
1.322TyrAsp: 1.322 ± 0.031
1.174TyrGlu: 1.174 ± 0.033
0.734TyrPhe: 0.734 ± 0.027
1.892TyrGly: 1.892 ± 0.04
0.444TyrHis: 0.444 ± 0.021
0.664TyrIle: 0.664 ± 0.025
0.336TyrLys: 0.336 ± 0.015
2.037TyrLeu: 2.037 ± 0.045
0.388TyrMet: 0.388 ± 0.02
0.442TyrAsn: 0.442 ± 0.02
0.973TyrPro: 0.973 ± 0.026
0.5TyrGln: 0.5 ± 0.023
1.571TyrArg: 1.571 ± 0.033
0.862TyrSer: 0.862 ± 0.029
1.009TyrThr: 1.009 ± 0.028
1.369TyrVal: 1.369 ± 0.03
0.344TyrTrp: 0.344 ± 0.018
0.442TyrTyr: 0.442 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4102 proteins (1297611 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski