Epsilonproteobacteria bacterium SCGC AD-311-E16

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; unclassified Epsilonproteobacteria

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 456 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P6UMQ4|A0A2P6UMQ4_9PROT Transglutaminase-like cysteine proteinase BTLCP OS=Epsilonproteobacteria bacterium SCGC AD-311-E16 OX=1449181 GN=BGW86_11016 PE=4 SV=1
MM1 pKa = 7.86IDD3 pKa = 4.39LNTIICGCNDD13 pKa = 3.31LSVRR17 pKa = 11.84DD18 pKa = 3.42IAEE21 pKa = 4.54CIKK24 pKa = 10.61EE25 pKa = 4.19NNFTTLDD32 pKa = 3.62EE33 pKa = 4.43LLEE36 pKa = 4.53NDD38 pKa = 4.06VCPMGDD44 pKa = 2.93KK45 pKa = 11.12CEE47 pKa = 4.12SCKK50 pKa = 11.01DD51 pKa = 3.12EE52 pKa = 5.07GYY54 pKa = 11.49NNDD57 pKa = 4.16GLNIPYY63 pKa = 10.03VLSIVAKK70 pKa = 10.53KK71 pKa = 10.66LII73 pKa = 4.01

Molecular weight: 8.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P6UN31|A0A2P6UN31_9PROT Uncharacterized protein OS=Epsilonproteobacteria bacterium SCGC AD-311-E16 OX=1449181 GN=BGW86_10612 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.32RR14 pKa = 11.84THH16 pKa = 6.0GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84NVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.39GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

456

0

456

133552

27

1297

292.9

33.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.645 ± 0.138

0.752 ± 0.043

5.863 ± 0.093

7.024 ± 0.122

4.753 ± 0.087

5.737 ± 0.109

2.006 ± 0.046

8.635 ± 0.114

8.311 ± 0.125

9.566 ± 0.117

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.845 ± 0.056

5.492 ± 0.087

2.746 ± 0.06

3.12 ± 0.064

3.303 ± 0.079

6.911 ± 0.09

5.295 ± 0.077

6.229 ± 0.087

0.821 ± 0.042

3.946 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski