Lepus americanus faeces associated genomovirus SHP9

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus lepam3

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5CJ37|A0A2Z5CJ37_9VIRU Capsid protein OS=Lepus americanus faeces associated genomovirus SHP9 OX=2219121 PE=4 SV=1
MM1 pKa = 7.82PFACNARR8 pKa = 11.84YY9 pKa = 8.97FLVTYY14 pKa = 9.34SHH16 pKa = 6.96VEE18 pKa = 3.77PLDD21 pKa = 3.62PFAVVEE27 pKa = 4.22HH28 pKa = 6.52FGNLGAEE35 pKa = 4.39VIVSLEE41 pKa = 4.23SYY43 pKa = 8.12NTTLGVHH50 pKa = 5.09YY51 pKa = 10.36HH52 pKa = 5.64VFADD56 pKa = 4.64FGRR59 pKa = 11.84KK60 pKa = 8.16FRR62 pKa = 11.84SRR64 pKa = 11.84RR65 pKa = 11.84TDD67 pKa = 2.68IFDD70 pKa = 3.27VDD72 pKa = 3.91GFHH75 pKa = 7.48PNISPSRR82 pKa = 11.84GTPQAGYY89 pKa = 10.56DD90 pKa = 3.74YY91 pKa = 11.05AVKK94 pKa = 10.52DD95 pKa = 4.0GNVVAGGLARR105 pKa = 11.84PSGVVAAGRR114 pKa = 11.84AAKK117 pKa = 8.68WHH119 pKa = 6.18QILDD123 pKa = 3.47AEE125 pKa = 4.47TRR127 pKa = 11.84DD128 pKa = 3.66EE129 pKa = 5.25FFALCEE135 pKa = 3.97EE136 pKa = 5.06LDD138 pKa = 3.88PEE140 pKa = 4.85RR141 pKa = 11.84LVCSFNQIQKK151 pKa = 9.25FADD154 pKa = 2.73WRR156 pKa = 11.84FAVEE160 pKa = 4.25PKK162 pKa = 10.37PYY164 pKa = 8.97ATPDD168 pKa = 3.43GVFDD172 pKa = 4.28LANYY176 pKa = 10.13GDD178 pKa = 4.02LSEE181 pKa = 4.54SKK183 pKa = 10.8SLILWGPSRR192 pKa = 11.84MGKK195 pKa = 6.86TVWARR200 pKa = 11.84SLGRR204 pKa = 11.84HH205 pKa = 6.15LYY207 pKa = 10.37FGGIFSARR215 pKa = 11.84NLGDD219 pKa = 3.46GGVDD223 pKa = 3.17YY224 pKa = 11.36AVFDD228 pKa = 5.51DD229 pKa = 3.63IAGGIKK235 pKa = 10.03FFPRR239 pKa = 11.84FKK241 pKa = 10.79DD242 pKa = 3.12WLGCQMEE249 pKa = 4.39FMVKK253 pKa = 9.1QMYY256 pKa = 9.85RR257 pKa = 11.84DD258 pKa = 3.38PHH260 pKa = 6.22LFRR263 pKa = 11.84WGKK266 pKa = 7.9PCIWVANTDD275 pKa = 3.43PRR277 pKa = 11.84HH278 pKa = 6.65DD279 pKa = 3.74MTHH282 pKa = 7.65DD283 pKa = 3.17EE284 pKa = 4.56VTWLEE289 pKa = 4.04ANCIFVEE296 pKa = 4.12ISSAIFHH303 pKa = 6.76ANIEE307 pKa = 4.2

Molecular weight: 34.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5CJ37|A0A2Z5CJ37_9VIRU Capsid protein OS=Lepus americanus faeces associated genomovirus SHP9 OX=2219121 PE=4 SV=1
MM1 pKa = 7.61AYY3 pKa = 10.1ARR5 pKa = 11.84KK6 pKa = 9.82RR7 pKa = 11.84YY8 pKa = 6.3TRR10 pKa = 11.84KK11 pKa = 9.59KK12 pKa = 7.83GAYY15 pKa = 8.95RR16 pKa = 11.84KK17 pKa = 8.44RR18 pKa = 11.84TATKK22 pKa = 10.06RR23 pKa = 11.84RR24 pKa = 11.84SSYY27 pKa = 10.42AKK29 pKa = 9.43KK30 pKa = 8.94RR31 pKa = 11.84TYY33 pKa = 10.46RR34 pKa = 11.84KK35 pKa = 8.87PAMSKK40 pKa = 10.33KK41 pKa = 10.33RR42 pKa = 11.84ILTVTSTKK50 pKa = 10.43KK51 pKa = 10.25RR52 pKa = 11.84DD53 pKa = 3.45TMLNWTNSTSSTQVGGTTYY72 pKa = 10.97GSAPAVITGGSNAQNCAAFLWCATARR98 pKa = 11.84DD99 pKa = 4.14ATVSSAGGPTTKK111 pKa = 10.42YY112 pKa = 10.89ALAGRR117 pKa = 11.84SATSCYY123 pKa = 9.66MVGLAEE129 pKa = 5.39KK130 pKa = 10.48IEE132 pKa = 4.2IQCNTGMPWQWRR144 pKa = 11.84RR145 pKa = 11.84ICFTMKK151 pKa = 10.32GVSLVPAATGSGTQFSDD168 pKa = 4.17FLEE171 pKa = 4.5TSNGYY176 pKa = 8.07MRR178 pKa = 11.84VMNQVNGNPSLDD190 pKa = 3.28PMYY193 pKa = 11.03SLFGALFKK201 pKa = 11.24GQVNSDD207 pKa = 2.95WQDD210 pKa = 2.91PMTAPTDD217 pKa = 3.57NSRR220 pKa = 11.84LSIKK224 pKa = 9.81YY225 pKa = 10.43DD226 pKa = 3.17KK227 pKa = 10.17VTSLASGNEE236 pKa = 3.89DD237 pKa = 3.44GFIRR241 pKa = 11.84SYY243 pKa = 11.1KK244 pKa = 9.57RR245 pKa = 11.84WHH247 pKa = 6.77PMNKK251 pKa = 8.21TLVYY255 pKa = 10.82NDD257 pKa = 4.58DD258 pKa = 3.83EE259 pKa = 5.64LGGGMLSNFKK269 pKa = 10.9SSFGKK274 pKa = 10.48AGMGDD279 pKa = 4.22YY280 pKa = 10.8YY281 pKa = 11.48VVDD284 pKa = 3.75IFRR287 pKa = 11.84ARR289 pKa = 11.84QGSATTDD296 pKa = 3.36QLSVCPEE303 pKa = 3.55ATLYY307 pKa = 9.32WHH309 pKa = 7.08EE310 pKa = 4.26KK311 pKa = 9.3

Molecular weight: 34.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

618

307

311

309.0

34.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.9 ± 0.075

1.942 ± 0.009

5.987 ± 1.077

3.722 ± 1.066

5.663 ± 1.774

8.576 ± 0.077

2.104 ± 1.059

3.56 ± 0.716

5.502 ± 1.373

5.987 ± 0.378

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.074 ± 0.801

4.207 ± 0.447

4.369 ± 0.603

2.589 ± 0.454

6.472 ± 0.203

7.443 ± 1.597

6.796 ± 2.299

6.311 ± 1.078

2.427 ± 0.128

4.369 ± 0.562
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski