Mycobacterium phage Dori

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8I4N9|G8I4N9_9CAUD Uncharacterized protein OS=Mycobacterium phage Dori OX=1089121 GN=32 PE=4 SV=1
MM1 pKa = 7.28TAPTPPADD9 pKa = 4.05AQWGIQWEE17 pKa = 4.61VFIQPDD23 pKa = 3.95GRR25 pKa = 11.84PGPPQQPEE33 pKa = 3.96YY34 pKa = 8.09TQPYY38 pKa = 8.4PVSYY42 pKa = 10.39DD43 pKa = 4.12DD44 pKa = 4.22NGQPIPLTEE53 pKa = 4.44DD54 pKa = 3.04QQTANAAAYY63 pKa = 8.91AQYY66 pKa = 11.25LSLLEE71 pKa = 3.91QWRR74 pKa = 11.84EE75 pKa = 3.78DD76 pKa = 2.98ITAYY80 pKa = 10.39DD81 pKa = 3.79AQTAALISDD90 pKa = 3.78DD91 pKa = 4.35ANWRR95 pKa = 11.84TTVFSAADD103 pKa = 3.45EE104 pKa = 4.31ATARR108 pKa = 11.84TMYY111 pKa = 10.28PIIKK115 pKa = 9.63QGNEE119 pKa = 3.78GNPQTRR125 pKa = 11.84NFILVWALPVNWTAAPEE142 pKa = 3.91

Molecular weight: 15.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8I4S7|G8I4S7_9CAUD Uncharacterized protein OS=Mycobacterium phage Dori OX=1089121 GN=70 PE=4 SV=1
MM1 pKa = 7.62AEE3 pKa = 3.58QWRR6 pKa = 11.84PVPGWEE12 pKa = 3.71GHH14 pKa = 5.27YY15 pKa = 10.01EE16 pKa = 3.87VSNRR20 pKa = 11.84GQVRR24 pKa = 11.84SVDD27 pKa = 3.42RR28 pKa = 11.84TVRR31 pKa = 11.84TATGALQTRR40 pKa = 11.84RR41 pKa = 11.84GRR43 pKa = 11.84VLTPFVRR50 pKa = 11.84KK51 pKa = 9.42HH52 pKa = 5.79GYY54 pKa = 8.85PVVGLYY60 pKa = 10.53GPNKK64 pKa = 9.89RR65 pKa = 11.84RR66 pKa = 11.84TQTDD70 pKa = 2.91VHH72 pKa = 7.51LLVLAAFAGLRR83 pKa = 11.84PDD85 pKa = 3.59GSVARR90 pKa = 11.84HH91 pKa = 5.67LNGDD95 pKa = 3.59PSDD98 pKa = 3.78NTIEE102 pKa = 3.92NLAWGTYY109 pKa = 7.37TEE111 pKa = 4.77NNDD114 pKa = 3.15DD115 pKa = 3.84QVRR118 pKa = 11.84HH119 pKa = 4.93GTHH122 pKa = 5.98YY123 pKa = 8.9EE124 pKa = 3.83ASRR127 pKa = 11.84TRR129 pKa = 11.84CDD131 pKa = 3.1RR132 pKa = 11.84GHH134 pKa = 7.17LLEE137 pKa = 4.93GEE139 pKa = 4.15NLAVRR144 pKa = 11.84TKK146 pKa = 10.7KK147 pKa = 10.38NGRR150 pKa = 11.84KK151 pKa = 9.05RR152 pKa = 11.84RR153 pKa = 11.84ACKK156 pKa = 9.69TCDD159 pKa = 3.41RR160 pKa = 11.84DD161 pKa = 3.08NSRR164 pKa = 11.84AYY166 pKa = 10.23RR167 pKa = 11.84ARR169 pKa = 11.84KK170 pKa = 7.81QRR172 pKa = 11.84KK173 pKa = 6.36STGRR177 pKa = 11.84AAA179 pKa = 3.91

Molecular weight: 20.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

20237

41

1901

215.3

23.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.077 ± 0.372

1.033 ± 0.148

6.34 ± 0.25

6.256 ± 0.281

2.53 ± 0.129

9.033 ± 0.55

1.967 ± 0.15

4.151 ± 0.171

2.906 ± 0.17

8.173 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.986 ± 0.116

2.876 ± 0.143

6.147 ± 0.187

3.602 ± 0.154

7.205 ± 0.433

5.159 ± 0.235

6.542 ± 0.225

7.906 ± 0.208

1.986 ± 0.142

2.125 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski