Pseudoalteromonas denitrificans DSM 6059

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas; Pseudoalteromonas denitrificans

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1T2I5|A0A1I1T2I5_9GAMM Biotin-lipoyl like OS=Pseudoalteromonas denitrificans DSM 6059 OX=1123010 GN=SAMN02745724_04771 PE=4 SV=1
MM1 pKa = 7.59SRR3 pKa = 11.84FSPVNFSKK11 pKa = 11.09YY12 pKa = 10.58LLLILTTSAVLFSTPSIAITISDD35 pKa = 3.51VSTTNFSNSDD45 pKa = 3.3HH46 pKa = 6.31YY47 pKa = 11.5QDD49 pKa = 5.55DD50 pKa = 4.24GNSLYY55 pKa = 10.6RR56 pKa = 11.84EE57 pKa = 4.06YY58 pKa = 11.24SSNLDD63 pKa = 2.86ITTVTNNNNDD73 pKa = 4.01AIINGHH79 pKa = 6.19LAWFHH84 pKa = 5.6GMRR87 pKa = 11.84VDD89 pKa = 4.36QPEE92 pKa = 4.54GPNFALIYY100 pKa = 9.0RR101 pKa = 11.84HH102 pKa = 6.13NIGYY106 pKa = 9.18EE107 pKa = 3.48ITFTVNDD114 pKa = 5.12PIEE117 pKa = 4.3EE118 pKa = 4.73GYY120 pKa = 10.46EE121 pKa = 3.73ISIEE125 pKa = 3.91QHH127 pKa = 4.91MKK129 pKa = 10.91GLITVSRR136 pKa = 11.84EE137 pKa = 3.53EE138 pKa = 4.73AISVSATAGLMLGRR152 pKa = 11.84VDD154 pKa = 3.57EE155 pKa = 4.62HH156 pKa = 8.14QGDD159 pKa = 4.53GPIHH163 pKa = 6.36LAGFSISGGGLSVDD177 pKa = 4.12SEE179 pKa = 4.49ATDD182 pKa = 3.65TVQTITIDD190 pKa = 3.49EE191 pKa = 4.59TDD193 pKa = 3.65TQNLATQYY201 pKa = 10.58IGTHH205 pKa = 5.24TFTMSFSSFPSPALVSIFQNSGGGEE230 pKa = 3.84TSIQFGVTSNQDD242 pKa = 2.59AFLYY246 pKa = 11.01GNATQNGLIDD256 pKa = 4.48LTPLGHH262 pKa = 6.9FSQINITSLNLTAPDD277 pKa = 3.68TDD279 pKa = 3.6NDD281 pKa = 4.01GVADD285 pKa = 4.6NIDD288 pKa = 3.65NCVLTSNPDD297 pKa = 3.41QLDD300 pKa = 3.49TDD302 pKa = 4.08GDD304 pKa = 4.5GVGDD308 pKa = 3.69VCDD311 pKa = 3.66NCMEE315 pKa = 4.54TPNSDD320 pKa = 4.37QLDD323 pKa = 3.74TDD325 pKa = 3.49EE326 pKa = 6.42DD327 pKa = 5.07GIGDD331 pKa = 3.77VCDD334 pKa = 3.7NCLDD338 pKa = 3.86TSNPDD343 pKa = 3.28QLDD346 pKa = 3.2IDD348 pKa = 4.0EE349 pKa = 5.64DD350 pKa = 4.94GIGDD354 pKa = 3.77VCDD357 pKa = 3.47NCMDD361 pKa = 4.23TSNPDD366 pKa = 3.2QLDD369 pKa = 3.2IDD371 pKa = 4.0EE372 pKa = 5.64DD373 pKa = 4.94GIGDD377 pKa = 3.77VCDD380 pKa = 3.48NCMDD384 pKa = 4.28TPNSDD389 pKa = 3.56QLDD392 pKa = 3.5IDD394 pKa = 3.78EE395 pKa = 5.67DD396 pKa = 4.94GIGDD400 pKa = 3.74VCDD403 pKa = 3.48NCMGTPNSDD412 pKa = 3.91QLDD415 pKa = 3.74TDD417 pKa = 3.6EE418 pKa = 6.34DD419 pKa = 4.72GVGDD423 pKa = 3.82VCDD426 pKa = 3.83NCVDD430 pKa = 3.8TSNPDD435 pKa = 3.54QVDD438 pKa = 3.43TDD440 pKa = 4.14DD441 pKa = 6.01DD442 pKa = 5.44GIGDD446 pKa = 3.75MCDD449 pKa = 2.95NCINTVNPDD458 pKa = 3.46QLDD461 pKa = 3.95SNNDD465 pKa = 3.21GTGDD469 pKa = 3.41LCEE472 pKa = 4.31QSDD475 pKa = 4.2EE476 pKa = 4.25QVTLIPKK483 pKa = 9.63KK484 pKa = 10.66VNCKK488 pKa = 6.27TLKK491 pKa = 10.71GNVPMTLLGSIDD503 pKa = 4.14FNVSSIDD510 pKa = 4.32ILTLDD515 pKa = 3.53INQVSVAEE523 pKa = 4.09KK524 pKa = 10.42HH525 pKa = 5.9NRR527 pKa = 11.84LHH529 pKa = 7.38LSDD532 pKa = 4.54INNDD536 pKa = 3.36GYY538 pKa = 11.81NDD540 pKa = 4.12AKK542 pKa = 10.69IHH544 pKa = 5.72LNKK547 pKa = 10.34SEE549 pKa = 4.09FCKK552 pKa = 10.86AMDD555 pKa = 4.22TLPAGNPKK563 pKa = 10.21EE564 pKa = 4.51FILNGQFGDD573 pKa = 3.69PSSSFEE579 pKa = 4.02STNSVFIKK587 pKa = 10.53KK588 pKa = 9.95

Molecular weight: 63.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1JWV7|A0A1I1JWV7_9GAMM Putative redox protein OS=Pseudoalteromonas denitrificans DSM 6059 OX=1123010 GN=SAMN02745724_01875 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.36RR14 pKa = 11.84SHH16 pKa = 6.15GFRR19 pKa = 11.84ARR21 pKa = 11.84MATTNGRR28 pKa = 11.84KK29 pKa = 9.47LINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5332

0

5332

1773304

39

4786

332.6

37.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.341 ± 0.038

1.017 ± 0.011

5.482 ± 0.03

5.896 ± 0.033

4.529 ± 0.022

6.011 ± 0.034

2.16 ± 0.018

7.538 ± 0.025

6.902 ± 0.036

10.46 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.307 ± 0.014

5.68 ± 0.032

3.425 ± 0.017

4.549 ± 0.031

3.528 ± 0.023

7.205 ± 0.031

5.51 ± 0.033

6.037 ± 0.03

1.121 ± 0.011

3.3 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski