Shigella phage vB_SdyM_006

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 252 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0IMP1|A0A3T0IMP1_9CAUD Baseplate wedge subunit and tail pin OS=Shigella phage vB_SdyM_006 OX=2500762 GN=vBSdyM006_144 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.15KK3 pKa = 8.53YY4 pKa = 8.83TVWCEE9 pKa = 3.62EE10 pKa = 4.29LEE12 pKa = 4.81LGFTTSNYY20 pKa = 10.02QLALDD25 pKa = 3.86IKK27 pKa = 10.77NNNTEE32 pKa = 4.16CIIDD36 pKa = 3.54EE37 pKa = 4.33SEE39 pKa = 4.48YY40 pKa = 10.75PGIAALAFDD49 pKa = 4.69FCDD52 pKa = 3.4LTEE55 pKa = 4.37DD56 pKa = 3.82TEE58 pKa = 4.28LTIIEE63 pKa = 4.39VKK65 pKa = 10.65

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0IMR5|A0A3T0IMR5_9CAUD Recombination repair and ssDNA binding protein OS=Shigella phage vB_SdyM_006 OX=2500762 GN=vBSdyM006_183 PE=4 SV=1
MM1 pKa = 7.5LLDD4 pKa = 4.62TISINSNALLRR15 pKa = 11.84IRR17 pKa = 11.84SLSDD21 pKa = 3.61LLYY24 pKa = 10.46QSCLVGNDD32 pKa = 3.08IKK34 pKa = 11.52LMLLYY39 pKa = 9.69FVRR42 pKa = 11.84ILHH45 pKa = 6.14TWPYY49 pKa = 8.36WIKK52 pKa = 10.37DD53 pKa = 3.26LAMIKK58 pKa = 10.41SKK60 pKa = 11.02LGTFNEE66 pKa = 3.83KK67 pKa = 9.88RR68 pKa = 11.84RR69 pKa = 11.84AIPAPITALLFFLIFMFVVMQIASQGISRR98 pKa = 11.84IFPDD102 pKa = 3.92TIFIKK107 pKa = 10.88LLLKK111 pKa = 10.82LLL113 pKa = 4.15

Molecular weight: 13.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

252

0

252

50962

54

1218

202.2

23.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.941 ± 0.158

1.224 ± 0.083

6.519 ± 0.122

7.384 ± 0.15

4.507 ± 0.106

5.645 ± 0.171

1.737 ± 0.075

7.969 ± 0.138

8.699 ± 0.172

8.4 ± 0.142

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.07

6.034 ± 0.111

3.35 ± 0.09

3.183 ± 0.091

3.544 ± 0.102

6.937 ± 0.105

5.565 ± 0.144

6.004 ± 0.101

1.258 ± 0.052

4.729 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski