Aphis glycines (Soybean aphid)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha;

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18358 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G0U8S4|A0A6G0U8S4_APHGL Uncharacterized protein OS=Aphis glycines OX=307491 GN=AGLY_000552 PE=4 SV=1
MM1 pKa = 7.52CPDD4 pKa = 3.61GTTCHH9 pKa = 6.21QNGEE13 pKa = 4.72CVRR16 pKa = 11.84STTGKK21 pKa = 9.94FSCEE25 pKa = 4.15CVVGWAGNGKK35 pKa = 8.99ICGPDD40 pKa = 3.39LDD42 pKa = 4.53LDD44 pKa = 3.93RR45 pKa = 11.84WPDD48 pKa = 3.08IDD50 pKa = 5.09LACSDD55 pKa = 4.09TRR57 pKa = 11.84CKK59 pKa = 10.44KK60 pKa = 10.29DD61 pKa = 3.08NCVRR65 pKa = 11.84VPNSGQEE72 pKa = 4.17DD73 pKa = 4.09ADD75 pKa = 3.71HH76 pKa = 7.41DD77 pKa = 5.11GIGDD81 pKa = 3.79VCDD84 pKa = 5.37DD85 pKa = 4.52DD86 pKa = 6.5ADD88 pKa = 4.05NDD90 pKa = 4.41GVPNTPDD97 pKa = 3.12NCPLVSNPDD106 pKa = 3.49QMDD109 pKa = 3.53SDD111 pKa = 4.06QDD113 pKa = 3.87GADD116 pKa = 3.81KK117 pKa = 11.19KK118 pKa = 11.36GDD120 pKa = 3.66ACDD123 pKa = 3.65NCPTIPNLDD132 pKa = 3.45QHH134 pKa = 6.76DD135 pKa = 4.08TDD137 pKa = 4.2NDD139 pKa = 5.07GIGDD143 pKa = 3.95ACDD146 pKa = 3.98ADD148 pKa = 3.68IDD150 pKa = 3.95NDD152 pKa = 4.64GILNHH157 pKa = 6.98EE158 pKa = 4.7DD159 pKa = 3.47NCPKK163 pKa = 10.36KK164 pKa = 10.77ANPDD168 pKa = 3.49QKK170 pKa = 10.88DD171 pKa = 3.19TDD173 pKa = 3.98GDD175 pKa = 4.14GLGDD179 pKa = 3.61VCDD182 pKa = 4.58NCPSMHH188 pKa = 6.76NPTQTDD194 pKa = 2.98SDD196 pKa = 3.84KK197 pKa = 11.68DD198 pKa = 4.3LIGDD202 pKa = 4.18ACDD205 pKa = 3.55SDD207 pKa = 3.39IDD209 pKa = 3.97RR210 pKa = 11.84DD211 pKa = 3.46RR212 pKa = 11.84DD213 pKa = 4.02GIQDD217 pKa = 4.22SVDD220 pKa = 3.45NCPKK224 pKa = 10.19VANSNQLDD232 pKa = 3.65TDD234 pKa = 3.87GDD236 pKa = 4.22GKK238 pKa = 11.27GDD240 pKa = 3.65EE241 pKa = 5.08CDD243 pKa = 3.85PDD245 pKa = 3.57IDD247 pKa = 5.09GDD249 pKa = 4.45GISNAQDD256 pKa = 2.92NCMLVYY262 pKa = 10.91NPDD265 pKa = 3.3QADD268 pKa = 3.37FDD270 pKa = 5.09GNGIGDD276 pKa = 3.97ICQDD280 pKa = 3.56DD281 pKa = 3.88NDD283 pKa = 3.91YY284 pKa = 11.75DD285 pKa = 3.88KK286 pKa = 11.76VPNHH290 pKa = 7.21LDD292 pKa = 3.27NCPNNSKK299 pKa = 10.26IFTTDD304 pKa = 3.09FRR306 pKa = 11.84TYY308 pKa = 8.31QTVVLDD314 pKa = 4.26PEE316 pKa = 5.01GDD318 pKa = 3.9SQIDD322 pKa = 4.05PNWVVYY328 pKa = 10.8NKK330 pKa = 10.33GAEE333 pKa = 4.33FVQTMNSDD341 pKa = 3.23PGLAVGYY348 pKa = 10.3DD349 pKa = 3.41SFGGVDD355 pKa = 3.87FEE357 pKa = 4.84GTFFVDD363 pKa = 3.1TDD365 pKa = 3.34IDD367 pKa = 3.87DD368 pKa = 5.12DD369 pKa = 4.35YY370 pKa = 11.94VGFVFSYY377 pKa = 10.52QDD379 pKa = 2.85NHH381 pKa = 6.64KK382 pKa = 10.12FYY384 pKa = 11.09TVMWKK389 pKa = 10.4KK390 pKa = 9.41GTQTYY395 pKa = 7.65WQATPFRR402 pKa = 11.84AVAEE406 pKa = 4.03PGIQIKK412 pKa = 10.05AVHH415 pKa = 6.25SEE417 pKa = 4.06TGPGQMLRR425 pKa = 11.84NALWNTEE432 pKa = 4.15STDD435 pKa = 3.19KK436 pKa = 10.48QARR439 pKa = 11.84II440 pKa = 3.86

Molecular weight: 47.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G0U6E5|A0A6G0U6E5_APHGL AMP-binding domain-containing protein OS=Aphis glycines OX=307491 GN=AGLY_002026 PE=4 SV=1
SS1 pKa = 6.95NLPIMKK7 pKa = 10.16LNNEE11 pKa = 3.9WNIFNTATQSFRR23 pKa = 11.84RR24 pKa = 11.84ILINKK29 pKa = 8.83ILSKK33 pKa = 11.19LKK35 pKa = 9.29MLKK38 pKa = 9.51IKK40 pKa = 10.55YY41 pKa = 9.18IYY43 pKa = 10.36NIINLMCTNNCTVRR57 pKa = 11.84VLKK60 pKa = 10.84NHH62 pKa = 6.45FPTATRR68 pKa = 11.84PRR70 pKa = 11.84QTLNCLLLASQQSEE84 pKa = 4.1RR85 pKa = 11.84KK86 pKa = 9.41IIPQNDD92 pKa = 3.17KK93 pKa = 9.64LTYY96 pKa = 9.14SVYY99 pKa = 9.62MQILTGTPSVSSDD112 pKa = 4.29CPCKK116 pKa = 10.7LCTLSLIRR124 pKa = 11.84LRR126 pKa = 11.84VCTARR131 pKa = 11.84GTHH134 pKa = 5.36VDD136 pKa = 3.07SRR138 pKa = 11.84ILIRR142 pKa = 11.84FPQYY146 pKa = 9.86PGQRR150 pKa = 11.84CTFGKK155 pKa = 10.15RR156 pKa = 11.84CTNAAHH162 pKa = 6.9LHH164 pKa = 6.27HH165 pKa = 6.76NLSSEE170 pKa = 4.0CCTTVRR176 pKa = 11.84AGRR179 pKa = 11.84SNRR182 pKa = 11.84TRR184 pKa = 11.84QFLCIPLPRR193 pKa = 11.84TLEE196 pKa = 4.22DD197 pKa = 3.37CSRR200 pKa = 11.84PGICVRR206 pKa = 11.84FPIRR210 pKa = 11.84RR211 pKa = 11.84IRR213 pKa = 11.84IRR215 pKa = 11.84GSTYY219 pKa = 10.24VPIFRR224 pKa = 11.84NSTLRR229 pKa = 11.84KK230 pKa = 9.69FGWSKK235 pKa = 11.09DD236 pKa = 3.41KK237 pKa = 10.74PIRR240 pKa = 11.84SCHH243 pKa = 6.3RR244 pKa = 11.84STSFWSLQNSMACSMSCSS262 pKa = 3.43

Molecular weight: 30.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18358

0

18358

8501140

170

16471

463.1

52.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.838 ± 0.018

2.189 ± 0.029

5.278 ± 0.016

5.763 ± 0.02

4.452 ± 0.017

4.587 ± 0.022

2.487 ± 0.01

7.163 ± 0.02

7.424 ± 0.024

9.235 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.008

6.474 ± 0.019

4.399 ± 0.027

3.898 ± 0.017

4.617 ± 0.016

8.229 ± 0.023

5.999 ± 0.017

5.929 ± 0.015

1.047 ± 0.006

3.687 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski