Sputnik virophage

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; Sputnikvirus; Mimivirus-dependent virus Sputnik

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|B4YNF9|V19_SPTNK Structural protein V19 OS=Sputnik virophage OX=543939 GN=ORF19 PE=1 SV=1
MM1 pKa = 7.56SYY3 pKa = 10.17SHH5 pKa = 7.1SIKK8 pKa = 10.57DD9 pKa = 3.68CQEE12 pKa = 4.27PDD14 pKa = 3.08TVYY17 pKa = 11.34YY18 pKa = 10.59DD19 pKa = 3.07ILIPFKK25 pKa = 10.96PNDD28 pKa = 3.51QGFSPAIFQAQLTQPIVHH46 pKa = 6.36NPSEE50 pKa = 4.37YY51 pKa = 9.73FLSVVRR57 pKa = 11.84FSIPTQNIPLTIPQIQPYY75 pKa = 10.32PNTNVNNTIYY85 pKa = 10.55SVSIGYY91 pKa = 9.81NGTYY95 pKa = 10.16SSQNFVQFDD104 pKa = 3.76PSLTSPNIPAPNAPTVTSPNVEE126 pKa = 4.02VTPYY130 pKa = 10.67YY131 pKa = 10.47YY132 pKa = 9.78IYY134 pKa = 10.37DD135 pKa = 3.76YY136 pKa = 11.15STFLQMINTALEE148 pKa = 3.83NAFNEE153 pKa = 3.73ISAPVGADD161 pKa = 2.89APFFFLL167 pKa = 5.58

Molecular weight: 18.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B4YNE2|V2_SPTNK Uncharacterized protein V2 OS=Sputnik virophage OX=543939 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.59SKK3 pKa = 9.87VQLYY7 pKa = 5.92GTPMRR12 pKa = 11.84GGCCGGCEE20 pKa = 4.49CPNCGFKK27 pKa = 10.87LGGVVAGVLAGGKK40 pKa = 8.22VSKK43 pKa = 10.14RR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 9.34SVAKK50 pKa = 10.26KK51 pKa = 10.62GGIGTKK57 pKa = 10.32GGAKK61 pKa = 9.69KK62 pKa = 10.68SPWVAFLQRR71 pKa = 11.84YY72 pKa = 8.31SKK74 pKa = 10.46EE75 pKa = 3.9HH76 pKa = 4.87GVKK79 pKa = 10.42YY80 pKa = 10.39SEE82 pKa = 5.0AMQSPKK88 pKa = 10.76AKK90 pKa = 10.05KK91 pKa = 9.71EE92 pKa = 3.55YY93 pKa = 10.31SALKK97 pKa = 10.4KK98 pKa = 10.11SGKK101 pKa = 6.35IHH103 pKa = 6.63KK104 pKa = 10.21VGGSKK109 pKa = 10.49SSGHH113 pKa = 6.64RR114 pKa = 11.84KK115 pKa = 6.66TKK117 pKa = 10.26KK118 pKa = 9.15PKK120 pKa = 9.89KK121 pKa = 9.25SMKK124 pKa = 10.31GGSKK128 pKa = 8.78TKK130 pKa = 10.41KK131 pKa = 10.13LSEE134 pKa = 3.98KK135 pKa = 10.56QLMKK139 pKa = 10.72EE140 pKa = 4.09LLAMM144 pKa = 5.16

Molecular weight: 15.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

4848

88

779

230.9

26.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.187 ± 0.255

1.093 ± 0.164

5.219 ± 0.45

6.106 ± 0.705

4.538 ± 0.425

6.333 ± 1.093

1.279 ± 0.125

7.426 ± 0.43

10.252 ± 1.15

8.478 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.191

7.776 ± 0.433

4.29 ± 0.44

3.63 ± 0.356

3.177 ± 0.401

6.745 ± 0.591

6.044 ± 0.616

5.507 ± 0.462

0.598 ± 0.093

5.116 ± 0.425
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski