Amino acid dipepetide frequency for Vagococcus humatus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.678AlaAla: 4.678 ± 0.131
0.687AlaCys: 0.687 ± 0.04
3.325AlaAsp: 3.325 ± 0.076
4.131AlaGlu: 4.131 ± 0.105
2.936AlaPhe: 2.936 ± 0.066
4.637AlaGly: 4.637 ± 0.113
1.261AlaHis: 1.261 ± 0.051
5.35AlaIle: 5.35 ± 0.098
5.178AlaLys: 5.178 ± 0.103
6.603AlaLeu: 6.603 ± 0.121
1.95AlaMet: 1.95 ± 0.062
3.063AlaAsn: 3.063 ± 0.075
1.953AlaPro: 1.953 ± 0.065
2.318AlaGln: 2.318 ± 0.066
2.282AlaArg: 2.282 ± 0.056
4.181AlaSer: 4.181 ± 0.088
4.07AlaThr: 4.07 ± 0.076
4.703AlaVal: 4.703 ± 0.106
0.544AlaTrp: 0.544 ± 0.035
2.609AlaTyr: 2.609 ± 0.067
0.0AlaXaa: 0.0 ± 0.0
Cys
0.41CysAla: 0.41 ± 0.024
0.093CysCys: 0.093 ± 0.014
0.373CysAsp: 0.373 ± 0.026
0.438CysGlu: 0.438 ± 0.029
0.365CysPhe: 0.365 ± 0.022
0.679CysGly: 0.679 ± 0.035
0.225CysHis: 0.225 ± 0.019
0.436CysIle: 0.436 ± 0.026
0.232CysLys: 0.232 ± 0.022
0.964CysLeu: 0.964 ± 0.042
0.191CysMet: 0.191 ± 0.017
0.219CysAsn: 0.219 ± 0.018
0.343CysPro: 0.343 ± 0.026
0.553CysGln: 0.553 ± 0.03
0.24CysArg: 0.24 ± 0.02
0.495CysSer: 0.495 ± 0.029
0.323CysThr: 0.323 ± 0.027
0.462CysVal: 0.462 ± 0.027
0.053CysTrp: 0.053 ± 0.01
0.352CysTyr: 0.352 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
2.9AspAla: 2.9 ± 0.07
0.397AspCys: 0.397 ± 0.027
2.144AspAsp: 2.144 ± 0.072
3.868AspGlu: 3.868 ± 0.09
2.63AspPhe: 2.63 ± 0.066
2.945AspGly: 2.945 ± 0.078
1.124AspHis: 1.124 ± 0.047
4.15AspIle: 4.15 ± 0.076
3.757AspLys: 3.757 ± 0.072
5.419AspLeu: 5.419 ± 0.094
1.584AspMet: 1.584 ± 0.049
2.126AspAsn: 2.126 ± 0.068
1.938AspPro: 1.938 ± 0.051
2.392AspGln: 2.392 ± 0.07
1.839AspArg: 1.839 ± 0.064
2.789AspSer: 2.789 ± 0.063
2.821AspThr: 2.821 ± 0.078
3.634AspVal: 3.634 ± 0.086
0.679AspTrp: 0.679 ± 0.037
2.57AspTyr: 2.57 ± 0.066
0.0AspXaa: 0.0 ± 0.0
Glu
5.446GluAla: 5.446 ± 0.126
0.384GluCys: 0.384 ± 0.026
3.793GluAsp: 3.793 ± 0.086
6.973GluGlu: 6.973 ± 0.125
2.426GluPhe: 2.426 ± 0.06
4.132GluGly: 4.132 ± 0.076
1.314GluHis: 1.314 ± 0.046
4.98GluIle: 4.98 ± 0.091
6.452GluLys: 6.452 ± 0.1
7.386GluLeu: 7.386 ± 0.12
2.146GluMet: 2.146 ± 0.051
3.228GluAsn: 3.228 ± 0.068
2.061GluPro: 2.061 ± 0.06
3.831GluGln: 3.831 ± 0.103
2.67GluArg: 2.67 ± 0.077
3.188GluSer: 3.188 ± 0.065
4.47GluThr: 4.47 ± 0.095
5.091GluVal: 5.091 ± 0.106
0.631GluTrp: 0.631 ± 0.036
2.061GluTyr: 2.061 ± 0.061
0.0GluXaa: 0.0 ± 0.0
Phe
2.533PheAla: 2.533 ± 0.068
0.349PheCys: 0.349 ± 0.026
2.416PheAsp: 2.416 ± 0.065
2.439PheGlu: 2.439 ± 0.062
2.273PhePhe: 2.273 ± 0.067
3.098PheGly: 3.098 ± 0.066
0.88PheHis: 0.88 ± 0.039
3.508PheIle: 3.508 ± 0.082
2.62PheLys: 2.62 ± 0.064
4.419PheLeu: 4.419 ± 0.09
1.181PheMet: 1.181 ± 0.043
1.985PheAsn: 1.985 ± 0.06
1.522PhePro: 1.522 ± 0.049
1.776PheGln: 1.776 ± 0.058
1.375PheArg: 1.375 ± 0.052
3.22PheSer: 3.22 ± 0.074
2.398PheThr: 2.398 ± 0.073
2.958PheVal: 2.958 ± 0.07
0.412PheTrp: 0.412 ± 0.029
1.892PheTyr: 1.892 ± 0.063
0.0PheXaa: 0.0 ± 0.0
Gly
4.378GlyAla: 4.378 ± 0.108
0.616GlyCys: 0.616 ± 0.03
3.098GlyAsp: 3.098 ± 0.075
4.301GlyGlu: 4.301 ± 0.085
3.101GlyPhe: 3.101 ± 0.077
4.472GlyGly: 4.472 ± 0.105
1.24GlyHis: 1.24 ± 0.051
5.705GlyIle: 5.705 ± 0.099
4.953GlyLys: 4.953 ± 0.1
6.802GlyLeu: 6.802 ± 0.121
2.123GlyMet: 2.123 ± 0.069
2.55GlyAsn: 2.55 ± 0.069
1.576GlyPro: 1.576 ± 0.049
2.683GlyGln: 2.683 ± 0.067
2.284GlyArg: 2.284 ± 0.064
3.922GlySer: 3.922 ± 0.089
3.914GlyThr: 3.914 ± 0.079
5.057GlyVal: 5.057 ± 0.105
0.629GlyTrp: 0.629 ± 0.039
2.845GlyTyr: 2.845 ± 0.079
0.0GlyXaa: 0.0 ± 0.0
His
1.137HisAla: 1.137 ± 0.045
0.171HisCys: 0.171 ± 0.015
0.946HisAsp: 0.946 ± 0.041
1.222HisGlu: 1.222 ± 0.042
1.057HisPhe: 1.057 ± 0.042
1.35HisGly: 1.35 ± 0.044
0.663HisHis: 0.663 ± 0.035
1.502HisIle: 1.502 ± 0.044
0.972HisLys: 0.972 ± 0.043
2.49HisLeu: 2.49 ± 0.068
0.55HisMet: 0.55 ± 0.028
0.719HisAsn: 0.719 ± 0.038
1.161HisPro: 1.161 ± 0.046
1.268HisGln: 1.268 ± 0.044
0.812HisArg: 0.812 ± 0.033
1.173HisSer: 1.173 ± 0.047
1.076HisThr: 1.076 ± 0.041
1.38HisVal: 1.38 ± 0.056
0.201HisTrp: 0.201 ± 0.019
1.015HisTyr: 1.015 ± 0.046
0.0HisXaa: 0.0 ± 0.0
Ile
5.445IleAla: 5.445 ± 0.121
0.693IleCys: 0.693 ± 0.035
4.137IleAsp: 4.137 ± 0.081
5.073IleGlu: 5.073 ± 0.089
3.069IlePhe: 3.069 ± 0.075
5.615IleGly: 5.615 ± 0.117
1.637IleHis: 1.637 ± 0.053
5.305IleIle: 5.305 ± 0.122
4.333IleLys: 4.333 ± 0.095
6.931IleLeu: 6.931 ± 0.128
1.734IleMet: 1.734 ± 0.057
3.079IleAsn: 3.079 ± 0.077
3.331IlePro: 3.331 ± 0.079
3.753IleGln: 3.753 ± 0.086
2.734IleArg: 2.734 ± 0.071
4.679IleSer: 4.679 ± 0.106
4.219IleThr: 4.219 ± 0.085
4.999IleVal: 4.999 ± 0.089
0.606IleTrp: 0.606 ± 0.033
2.675IleTyr: 2.675 ± 0.071
0.0IleXaa: 0.0 ± 0.0
Lys
4.258LysAla: 4.258 ± 0.099
0.283LysCys: 0.283 ± 0.02
3.923LysAsp: 3.923 ± 0.089
7.917LysGlu: 7.917 ± 0.129
1.81LysPhe: 1.81 ± 0.051
4.222LysGly: 4.222 ± 0.09
1.305LysHis: 1.305 ± 0.05
4.835LysIle: 4.835 ± 0.093
6.275LysLys: 6.275 ± 0.116
5.652LysLeu: 5.652 ± 0.098
2.135LysMet: 2.135 ± 0.067
3.351LysAsn: 3.351 ± 0.08
2.228LysPro: 2.228 ± 0.059
4.314LysGln: 4.314 ± 0.099
3.013LysArg: 3.013 ± 0.079
3.116LysSer: 3.116 ± 0.067
3.891LysThr: 3.891 ± 0.073
4.477LysVal: 4.477 ± 0.088
0.647LysTrp: 0.647 ± 0.035
2.044LysTyr: 2.044 ± 0.055
0.0LysXaa: 0.0 ± 0.0
Leu
8.388LeuAla: 8.388 ± 0.133
0.669LeuCys: 0.669 ± 0.034
5.665LeuAsp: 5.665 ± 0.1
6.992LeuGlu: 6.992 ± 0.128
4.534LeuPhe: 4.534 ± 0.092
6.912LeuGly: 6.912 ± 0.123
1.711LeuHis: 1.711 ± 0.05
7.137LeuIle: 7.137 ± 0.137
6.683LeuLys: 6.683 ± 0.119
10.473LeuLeu: 10.473 ± 0.194
2.644LeuMet: 2.644 ± 0.064
4.435LeuAsn: 4.435 ± 0.084
4.356LeuPro: 4.356 ± 0.085
3.558LeuGln: 3.558 ± 0.081
3.164LeuArg: 3.164 ± 0.073
6.782LeuSer: 6.782 ± 0.117
7.222LeuThr: 7.222 ± 0.112
7.156LeuVal: 7.156 ± 0.119
0.711LeuTrp: 0.711 ± 0.035
3.178LeuTyr: 3.178 ± 0.069
0.0LeuXaa: 0.0 ± 0.0
Met
2.004MetAla: 2.004 ± 0.062
0.191MetCys: 0.191 ± 0.018
1.522MetAsp: 1.522 ± 0.051
1.753MetGlu: 1.753 ± 0.056
1.015MetPhe: 1.015 ± 0.044
1.787MetGly: 1.787 ± 0.051
0.418MetHis: 0.418 ± 0.027
2.208MetIle: 2.208 ± 0.06
2.35MetLys: 2.35 ± 0.057
2.474MetLeu: 2.474 ± 0.065
0.896MetMet: 0.896 ± 0.043
1.395MetAsn: 1.395 ± 0.053
0.981MetPro: 0.981 ± 0.037
0.888MetGln: 0.888 ± 0.04
0.981MetArg: 0.981 ± 0.033
1.819MetSer: 1.819 ± 0.054
1.964MetThr: 1.964 ± 0.056
1.771MetVal: 1.771 ± 0.057
0.18MetTrp: 0.18 ± 0.018
0.772MetTyr: 0.772 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
2.202AsnAla: 2.202 ± 0.062
0.281AsnCys: 0.281 ± 0.021
1.932AsnAsp: 1.932 ± 0.055
2.886AsnGlu: 2.886 ± 0.079
1.723AsnPhe: 1.723 ± 0.052
2.781AsnGly: 2.781 ± 0.072
1.2AsnHis: 1.2 ± 0.051
3.116AsnIle: 3.116 ± 0.073
2.767AsnLys: 2.767 ± 0.071
4.042AsnLeu: 4.042 ± 0.081
1.308AsnMet: 1.308 ± 0.047
1.797AsnAsn: 1.797 ± 0.061
2.059AsnPro: 2.059 ± 0.052
3.219AsnGln: 3.219 ± 0.087
1.864AsnArg: 1.864 ± 0.052
2.07AsnSer: 2.07 ± 0.062
1.95AsnThr: 1.95 ± 0.056
2.614AsnVal: 2.614 ± 0.06
0.574AsnTrp: 0.574 ± 0.032
1.893AsnTyr: 1.893 ± 0.057
0.0AsnXaa: 0.0 ± 0.0
Pro
2.125ProAla: 2.125 ± 0.061
0.222ProCys: 0.222 ± 0.02
2.104ProAsp: 2.104 ± 0.065
2.891ProGlu: 2.891 ± 0.063
1.732ProPhe: 1.732 ± 0.048
2.089ProGly: 2.089 ± 0.066
0.772ProHis: 0.772 ± 0.037
2.895ProIle: 2.895 ± 0.062
2.741ProLys: 2.741 ± 0.079
3.59ProLeu: 3.59 ± 0.084
0.88ProMet: 0.88 ± 0.037
1.84ProAsn: 1.84 ± 0.059
0.698ProPro: 0.698 ± 0.038
1.229ProGln: 1.229 ± 0.047
0.983ProArg: 0.983 ± 0.037
2.303ProSer: 2.303 ± 0.053
2.496ProThr: 2.496 ± 0.067
3.034ProVal: 3.034 ± 0.068
0.317ProTrp: 0.317 ± 0.025
1.56ProTyr: 1.56 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
3.891GlnAla: 3.891 ± 0.083
0.224GlnCys: 0.224 ± 0.019
2.213GlnAsp: 2.213 ± 0.065
3.648GlnGlu: 3.648 ± 0.095
1.802GlnPhe: 1.802 ± 0.049
2.828GlnGly: 2.828 ± 0.069
1.034GlnHis: 1.034 ± 0.037
3.04GlnIle: 3.04 ± 0.079
3.248GlnLys: 3.248 ± 0.082
6.128GlnLeu: 6.128 ± 0.138
1.226GlnMet: 1.226 ± 0.042
1.713GlnAsn: 1.713 ± 0.051
1.765GlnPro: 1.765 ± 0.06
2.903GlnGln: 2.903 ± 0.109
1.66GlnArg: 1.66 ± 0.054
2.189GlnSer: 2.189 ± 0.061
2.826GlnThr: 2.826 ± 0.073
4.124GlnVal: 4.124 ± 0.079
0.441GlnTrp: 0.441 ± 0.028
1.465GlnTyr: 1.465 ± 0.051
0.0GlnXaa: 0.0 ± 0.0
Arg
1.966ArgAla: 1.966 ± 0.061
0.273ArgCys: 0.273 ± 0.02
1.629ArgAsp: 1.629 ± 0.058
2.709ArgGlu: 2.709 ± 0.077
1.623ArgPhe: 1.623 ± 0.055
2.015ArgGly: 2.015 ± 0.059
0.835ArgHis: 0.835 ± 0.037
2.638ArgIle: 2.638 ± 0.074
2.715ArgLys: 2.715 ± 0.07
3.93ArgLeu: 3.93 ± 0.081
1.091ArgMet: 1.091 ± 0.042
1.387ArgAsn: 1.387 ± 0.046
1.25ArgPro: 1.25 ± 0.046
2.098ArgGln: 2.098 ± 0.066
1.687ArgArg: 1.687 ± 0.056
1.837ArgSer: 1.837 ± 0.054
1.782ArgThr: 1.782 ± 0.053
2.524ArgVal: 2.524 ± 0.07
0.27ArgTrp: 0.27 ± 0.021
1.641ArgTyr: 1.641 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
3.172SerAla: 3.172 ± 0.077
0.402SerCys: 0.402 ± 0.023
2.969SerAsp: 2.969 ± 0.061
3.597SerGlu: 3.597 ± 0.073
3.1SerPhe: 3.1 ± 0.072
4.121SerGly: 4.121 ± 0.083
1.472SerHis: 1.472 ± 0.052
4.375SerIle: 4.375 ± 0.102
3.73SerLys: 3.73 ± 0.078
6.585SerLeu: 6.585 ± 0.122
1.461SerMet: 1.461 ± 0.051
2.244SerAsn: 2.244 ± 0.063
1.943SerPro: 1.943 ± 0.055
3.24SerGln: 3.24 ± 0.073
2.122SerArg: 2.122 ± 0.057
3.605SerSer: 3.605 ± 0.08
3.001SerThr: 3.001 ± 0.064
4.184SerVal: 4.184 ± 0.081
0.513SerTrp: 0.513 ± 0.027
2.506SerTyr: 2.506 ± 0.077
0.0SerXaa: 0.0 ± 0.0
Thr
3.476ThrAla: 3.476 ± 0.078
0.5ThrCys: 0.5 ± 0.033
3.322ThrAsp: 3.322 ± 0.08
4.07ThrGlu: 4.07 ± 0.085
2.681ThrPhe: 2.681 ± 0.067
4.568ThrGly: 4.568 ± 0.084
1.388ThrHis: 1.388 ± 0.049
4.419ThrIle: 4.419 ± 0.084
3.815ThrLys: 3.815 ± 0.082
5.964ThrLeu: 5.964 ± 0.102
1.314ThrMet: 1.314 ± 0.045
2.554ThrAsn: 2.554 ± 0.063
2.633ThrPro: 2.633 ± 0.078
2.377ThrGln: 2.377 ± 0.068
1.877ThrArg: 1.877 ± 0.058
3.682ThrSer: 3.682 ± 0.077
3.436ThrThr: 3.436 ± 0.08
4.062ThrVal: 4.062 ± 0.084
0.553ThrTrp: 0.553 ± 0.034
2.406ThrTyr: 2.406 ± 0.057
0.0ThrXaa: 0.0 ± 0.0
Val
5.454ValAla: 5.454 ± 0.098
0.643ValCys: 0.643 ± 0.034
3.643ValAsp: 3.643 ± 0.088
4.644ValGlu: 4.644 ± 0.102
3.019ValPhe: 3.019 ± 0.069
4.89ValGly: 4.89 ± 0.102
1.152ValHis: 1.152 ± 0.048
5.496ValIle: 5.496 ± 0.097
4.602ValLys: 4.602 ± 0.094
6.876ValLeu: 6.876 ± 0.118
1.866ValMet: 1.866 ± 0.059
2.776ValAsn: 2.776 ± 0.074
2.767ValPro: 2.767 ± 0.068
2.336ValGln: 2.336 ± 0.067
2.326ValArg: 2.326 ± 0.062
4.851ValSer: 4.851 ± 0.091
4.729ValThr: 4.729 ± 0.087
5.2ValVal: 5.2 ± 0.106
0.542ValTrp: 0.542 ± 0.028
2.554ValTyr: 2.554 ± 0.068
0.0ValXaa: 0.0 ± 0.0
Trp
0.426TrpAla: 0.426 ± 0.028
0.069TrpCys: 0.069 ± 0.01
0.483TrpAsp: 0.483 ± 0.032
0.594TrpGlu: 0.594 ± 0.035
0.476TrpPhe: 0.476 ± 0.029
0.502TrpGly: 0.502 ± 0.033
0.199TrpHis: 0.199 ± 0.017
0.621TrpIle: 0.621 ± 0.031
0.513TrpLys: 0.513 ± 0.031
1.261TrpLeu: 1.261 ± 0.056
0.272TrpMet: 0.272 ± 0.022
0.399TrpAsn: 0.399 ± 0.029
0.251TrpPro: 0.251 ± 0.021
0.727TrpGln: 0.727 ± 0.037
0.325TrpArg: 0.325 ± 0.026
0.473TrpSer: 0.473 ± 0.027
0.431TrpThr: 0.431 ± 0.029
0.537TrpVal: 0.537 ± 0.032
0.101TrpTrp: 0.101 ± 0.013
0.325TrpTyr: 0.325 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.165TyrAla: 2.165 ± 0.056
0.296TyrCys: 0.296 ± 0.023
2.072TyrAsp: 2.072 ± 0.059
2.519TyrGlu: 2.519 ± 0.059
1.882TyrPhe: 1.882 ± 0.059
2.606TyrGly: 2.606 ± 0.073
1.033TyrHis: 1.033 ± 0.04
2.332TyrIle: 2.332 ± 0.064
1.758TyrLys: 1.758 ± 0.05
4.382TyrLeu: 4.382 ± 0.11
0.819TyrMet: 0.819 ± 0.039
1.419TyrAsn: 1.419 ± 0.051
1.687TyrPro: 1.687 ± 0.053
2.907TyrGln: 2.907 ± 0.087
1.662TyrArg: 1.662 ± 0.055
1.967TyrSer: 1.967 ± 0.061
2.078TyrThr: 2.078 ± 0.065
2.402TyrVal: 2.402 ± 0.065
0.381TyrTrp: 0.381 ± 0.024
1.668TyrTyr: 1.668 ± 0.056
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1964 proteins (621691 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski