Thiomicrorhabdus sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Thiomicrorhabdus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2078 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1HI22|A0A4V1HI22_9GAMM Formate-dependent phosphoribosylglycinamide formyltransferase OS=Thiomicrorhabdus sediminis OX=2580412 GN=purT PE=3 SV=1
MM1 pKa = 7.73INRR4 pKa = 11.84SKK6 pKa = 10.83QGGFSLVEE14 pKa = 3.9MMVVLAILGILFVGVTEE31 pKa = 4.53GMKK34 pKa = 10.32SFQEE38 pKa = 4.27TEE40 pKa = 3.82LGKK43 pKa = 11.02SNNEE47 pKa = 3.67KK48 pKa = 10.68LDD50 pKa = 3.72LVKK53 pKa = 10.66QQLLKK58 pKa = 10.51FVQAEE63 pKa = 4.44KK64 pKa = 11.27YY65 pKa = 10.1LLCPDD70 pKa = 3.98SDD72 pKa = 3.74GDD74 pKa = 3.97GYY76 pKa = 10.7EE77 pKa = 3.83NRR79 pKa = 11.84TPSAVVIGTLGNVQACTVSYY99 pKa = 8.64GTVPYY104 pKa = 10.17RR105 pKa = 11.84DD106 pKa = 3.9LGLKK110 pKa = 9.96EE111 pKa = 4.13SQAVDD116 pKa = 2.68AWNNAIAYY124 pKa = 7.91AVNTQTTDD132 pKa = 2.91AQKK135 pKa = 10.5ICDD138 pKa = 3.75KK139 pKa = 10.98TEE141 pKa = 3.89AASMFCNLVPGVLWFSLADD160 pKa = 3.92TPPLAINRR168 pKa = 11.84GDD170 pKa = 3.31GNYY173 pKa = 9.84YY174 pKa = 9.18ICKK177 pKa = 10.14LGVAQCDD184 pKa = 3.36ATFVLDD190 pKa = 4.24SNNVLQDD197 pKa = 3.31ATVVLVAFNQTGQQAWDD214 pKa = 3.76DD215 pKa = 3.98CSEE218 pKa = 4.41LSTSQQEE225 pKa = 4.09NCDD228 pKa = 3.23ADD230 pKa = 4.98LYY232 pKa = 9.57YY233 pKa = 10.64QKK235 pKa = 10.61QSYY238 pKa = 10.54SSGGVIDD245 pKa = 5.6DD246 pKa = 5.31DD247 pKa = 4.84QIQTINGYY255 pKa = 7.6EE256 pKa = 3.98IKK258 pKa = 10.19ALAMGTVMTWNAFDD272 pKa = 4.18SASSAADD279 pKa = 3.34LTPTYY284 pKa = 10.4EE285 pKa = 4.45AFDD288 pKa = 3.49IAAGDD293 pKa = 4.25DD294 pKa = 3.75VSSLYY299 pKa = 11.14SSDD302 pKa = 3.4SDD304 pKa = 3.54VVMINHH310 pKa = 7.08DD311 pKa = 2.96VDD313 pKa = 3.43QAVRR317 pKa = 11.84LGNGDD322 pKa = 3.99DD323 pKa = 3.85YY324 pKa = 11.51MVIGNDD330 pKa = 3.25LNANANLALNKK341 pKa = 10.61GNDD344 pKa = 3.14SLYY347 pKa = 10.32IVGSSYY353 pKa = 11.86SNVNLGLGDD362 pKa = 3.67DD363 pKa = 4.05TMVLGGDD370 pKa = 3.58LTNNLSAQAGNDD382 pKa = 3.5RR383 pKa = 11.84VWIQGSVLSGSSLDD397 pKa = 3.78LGKK400 pKa = 10.3NDD402 pKa = 4.06DD403 pKa = 4.1VLWLGKK409 pKa = 9.91SDD411 pKa = 3.93NADD414 pKa = 3.15SGQIFTTLQGGDD426 pKa = 3.48GYY428 pKa = 11.15DD429 pKa = 2.54IVVFEE434 pKa = 4.94NQASWSDD441 pKa = 3.45LSASEE446 pKa = 4.78QSNLQNFEE454 pKa = 3.85LAIFKK459 pKa = 9.72TGSDD463 pKa = 3.58GGSGRR468 pKa = 11.84AYY470 pKa = 10.63CFLDD474 pKa = 4.23GSSPSCYY481 pKa = 10.65

Molecular weight: 51.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P9K332|A0A4P9K332_9GAMM Malonyl-[acyl-carrier protein] O-methyltransferase OS=Thiomicrorhabdus sediminis OX=2580412 GN=bioC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.16VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.98RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2078

0

2078

695024

41

2244

334.5

37.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.782 ± 0.064

0.856 ± 0.015

5.694 ± 0.048

6.44 ± 0.048

4.098 ± 0.038

6.465 ± 0.049

2.233 ± 0.029

6.588 ± 0.043

5.6 ± 0.04

10.439 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.772 ± 0.027

4.46 ± 0.039

3.761 ± 0.031

5.438 ± 0.064

4.289 ± 0.04

6.279 ± 0.043

4.871 ± 0.042

6.743 ± 0.048

1.203 ± 0.021

2.989 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski