Streptomyces phage Dagobah

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G4AWR0|A0A7G4AWR0_9VIRU Uncharacterized protein OS=Streptomyces phage Dagobah OX=2740180 GN=HUN42_00073 PE=4 SV=1
MM1 pKa = 7.38SLSAWCTCCGDD12 pKa = 3.51EE13 pKa = 4.54FDD15 pKa = 5.51PIEE18 pKa = 4.64GVTVPQDD25 pKa = 3.2PDD27 pKa = 3.2EE28 pKa = 4.66PRR30 pKa = 11.84FCSTGCEE37 pKa = 4.14EE38 pKa = 4.25RR39 pKa = 11.84WWAAVDD45 pKa = 3.75AEE47 pKa = 4.28EE48 pKa = 5.92AYY50 pKa = 10.6DD51 pKa = 3.94AGVGGAPVFPP61 pKa = 5.33

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G4AWJ5|A0A7G4AWJ5_9VIRU Putative cysteinyl-tRNA synthetase OS=Streptomyces phage Dagobah OX=2740180 GN=HUN42_00007 PE=4 SV=1
MM1 pKa = 7.73PKK3 pKa = 10.34CPEE6 pKa = 3.67NCTCGRR12 pKa = 11.84HH13 pKa = 5.09RR14 pKa = 11.84SKK16 pKa = 10.93GAQRR20 pKa = 11.84SAEE23 pKa = 3.75VRR25 pKa = 11.84ARR27 pKa = 11.84ISAAKK32 pKa = 9.09MGHH35 pKa = 5.37EE36 pKa = 4.39VSAEE40 pKa = 3.57TRR42 pKa = 11.84ARR44 pKa = 11.84ISAAGKK50 pKa = 9.78GRR52 pKa = 11.84KK53 pKa = 8.62QSAEE57 pKa = 3.91HH58 pKa = 5.71ARR60 pKa = 11.84SRR62 pKa = 11.84AEE64 pKa = 3.55SRR66 pKa = 11.84KK67 pKa = 7.16THH69 pKa = 4.15GHH71 pKa = 5.18AAKK74 pKa = 10.72VNGRR78 pKa = 11.84RR79 pKa = 11.84SPEE82 pKa = 3.54YY83 pKa = 10.17RR84 pKa = 11.84AWDD87 pKa = 3.43AMKK90 pKa = 10.18QRR92 pKa = 11.84CHH94 pKa = 6.11NPRR97 pKa = 11.84SRR99 pKa = 11.84QYY101 pKa = 9.78AQYY104 pKa = 9.03GARR107 pKa = 11.84GIYY110 pKa = 10.16VCDD113 pKa = 3.08RR114 pKa = 11.84WRR116 pKa = 11.84NSFEE120 pKa = 4.37NFLADD125 pKa = 3.35MGPRR129 pKa = 11.84PSVPGVRR136 pKa = 11.84YY137 pKa = 10.09SVDD140 pKa = 3.91RR141 pKa = 11.84IDD143 pKa = 4.45NDD145 pKa = 3.8GPYY148 pKa = 10.38SPEE151 pKa = 3.55NCRR154 pKa = 11.84WATMSEE160 pKa = 4.27QNRR163 pKa = 11.84NRR165 pKa = 11.84PNFNPNKK172 pKa = 10.16RR173 pKa = 11.84GG174 pKa = 3.35

Molecular weight: 19.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

14145

29

1278

158.9

17.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.548 ± 0.303

1.202 ± 0.186

6.539 ± 0.311

6.469 ± 0.397

2.927 ± 0.193

9.233 ± 0.253

2.114 ± 0.146

3.69 ± 0.196

3.344 ± 0.264

7.741 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.17 ± 0.126

2.835 ± 0.205

5.422 ± 0.275

3.351 ± 0.22

7.833 ± 0.374

5.917 ± 0.278

6.214 ± 0.279

7.798 ± 0.263

2.008 ± 0.141

2.644 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski