Anoxybacillus amylolyticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Anoxybacillus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3186 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160F4A4|A0A160F4A4_9BACI TubC_N domain-containing protein OS=Anoxybacillus amylolyticus OX=294699 GN=GFC30_2761 PE=4 SV=1
MM1 pKa = 7.28EE2 pKa = 5.9HH3 pKa = 6.64GEE5 pKa = 3.93KK6 pKa = 10.35HH7 pKa = 4.36ITVVDD12 pKa = 3.33EE13 pKa = 4.72HH14 pKa = 8.52GNEE17 pKa = 4.02QLCEE21 pKa = 3.82VLFTFEE27 pKa = 5.35SEE29 pKa = 4.67DD30 pKa = 3.47FGKK33 pKa = 10.64SYY35 pKa = 11.12VFYY38 pKa = 10.57YY39 pKa = 10.21PIGAEE44 pKa = 3.88YY45 pKa = 10.49DD46 pKa = 3.75DD47 pKa = 4.58EE48 pKa = 5.05EE49 pKa = 4.28EE50 pKa = 4.44TEE52 pKa = 3.96IHH54 pKa = 6.29VSAFVPGEE62 pKa = 3.88GDD64 pKa = 3.72QEE66 pKa = 5.26GEE68 pKa = 4.06LQPIEE73 pKa = 4.44TEE75 pKa = 4.44EE76 pKa = 4.01EE77 pKa = 3.31WDD79 pKa = 3.66MIEE82 pKa = 4.42EE83 pKa = 4.17VWNTFCAEE91 pKa = 3.97QEE93 pKa = 4.12EE94 pKa = 4.74DD95 pKa = 3.73EE96 pKa = 4.47EE97 pKa = 4.5

Molecular weight: 11.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A167TMS7|A0A167TMS7_9BACI Uncharacterized protein OS=Anoxybacillus amylolyticus OX=294699 GN=GFC30_2038 PE=4 SV=1
MM1 pKa = 7.41LRR3 pKa = 11.84LFMFGRR9 pKa = 11.84NRR11 pKa = 11.84TLWNQWLRR19 pKa = 11.84LTGQRR24 pKa = 11.84NSRR27 pKa = 11.84GMLWSLISFGLGIAATTAFNWRR49 pKa = 11.84RR50 pKa = 11.84QQMAQSTFKK59 pKa = 10.87RR60 pKa = 11.84PIRR63 pKa = 11.84RR64 pKa = 11.84AMRR67 pKa = 11.84TINGFMNRR75 pKa = 11.84RR76 pKa = 11.84MRR78 pKa = 11.84RR79 pKa = 11.84PSLAAVEE86 pKa = 4.04LSKK89 pKa = 11.04EE90 pKa = 3.9IAEE93 pKa = 4.41KK94 pKa = 10.67PKK96 pKa = 10.36IPTNNRR102 pKa = 11.84SS103 pKa = 3.03

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3186

0

3186

897943

29

1698

281.8

31.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.756 ± 0.049

0.832 ± 0.012

4.63 ± 0.033

7.565 ± 0.051

4.412 ± 0.035

6.623 ± 0.04

2.369 ± 0.021

7.132 ± 0.038

6.673 ± 0.038

9.82 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.784 ± 0.022

3.614 ± 0.028

3.792 ± 0.031

4.167 ± 0.031

4.883 ± 0.031

5.154 ± 0.031

5.475 ± 0.032

7.62 ± 0.041

1.122 ± 0.016

3.577 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski