Amino acid dipepetide frequency for Pasteurella phage PHB01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.448AlaAla: 4.448 ± 0.672
0.872AlaCys: 0.872 ± 0.243
4.797AlaAsp: 4.797 ± 0.506
5.495AlaGlu: 5.495 ± 0.805
3.314AlaPhe: 3.314 ± 0.493
4.099AlaGly: 4.099 ± 0.612
1.134AlaHis: 1.134 ± 0.263
3.837AlaIle: 3.837 ± 0.615
5.669AlaLys: 5.669 ± 0.678
6.803AlaLeu: 6.803 ± 0.941
1.395AlaMet: 1.395 ± 0.446
4.535AlaAsn: 4.535 ± 0.75
1.744AlaPro: 1.744 ± 0.367
3.401AlaGln: 3.401 ± 0.579
4.099AlaArg: 4.099 ± 0.579
4.971AlaSer: 4.971 ± 0.815
3.925AlaThr: 3.925 ± 0.635
5.407AlaVal: 5.407 ± 0.774
1.395AlaTrp: 1.395 ± 0.398
2.18AlaTyr: 2.18 ± 0.515
0.0AlaXaa: 0.0 ± 0.0
Cys
0.698CysAla: 0.698 ± 0.213
0.0CysCys: 0.0 ± 0.0
0.349CysAsp: 0.349 ± 0.215
0.698CysGlu: 0.698 ± 0.219
0.523CysPhe: 0.523 ± 0.224
0.523CysGly: 0.523 ± 0.206
0.349CysHis: 0.349 ± 0.195
0.698CysIle: 0.698 ± 0.275
0.698CysLys: 0.698 ± 0.277
0.872CysLeu: 0.872 ± 0.28
0.087CysMet: 0.087 ± 0.085
0.436CysAsn: 0.436 ± 0.195
0.523CysPro: 0.523 ± 0.19
0.436CysGln: 0.436 ± 0.195
0.436CysArg: 0.436 ± 0.221
0.349CysSer: 0.349 ± 0.198
0.436CysThr: 0.436 ± 0.266
0.611CysVal: 0.611 ± 0.211
0.0CysTrp: 0.0 ± 0.0
0.174CysTyr: 0.174 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
3.663AspAla: 3.663 ± 0.579
0.872AspCys: 0.872 ± 0.345
3.576AspAsp: 3.576 ± 0.464
4.448AspGlu: 4.448 ± 0.699
3.489AspPhe: 3.489 ± 0.583
5.495AspGly: 5.495 ± 0.504
1.134AspHis: 1.134 ± 0.344
4.535AspIle: 4.535 ± 0.505
5.756AspLys: 5.756 ± 0.719
4.274AspLeu: 4.274 ± 0.357
1.047AspMet: 1.047 ± 0.277
3.227AspAsn: 3.227 ± 0.429
2.529AspPro: 2.529 ± 0.545
1.657AspGln: 1.657 ± 0.348
2.18AspArg: 2.18 ± 0.274
3.75AspSer: 3.75 ± 0.471
4.622AspThr: 4.622 ± 0.453
3.489AspVal: 3.489 ± 0.501
0.611AspTrp: 0.611 ± 0.214
2.006AspTyr: 2.006 ± 0.335
0.0AspXaa: 0.0 ± 0.0
Glu
5.931GluAla: 5.931 ± 0.856
0.611GluCys: 0.611 ± 0.236
4.535GluAsp: 4.535 ± 0.624
6.977GluGlu: 6.977 ± 0.942
2.442GluPhe: 2.442 ± 0.482
3.401GluGly: 3.401 ± 0.691
1.395GluHis: 1.395 ± 0.479
3.663GluIle: 3.663 ± 0.601
4.448GluLys: 4.448 ± 0.704
5.843GluLeu: 5.843 ± 0.81
2.18GluMet: 2.18 ± 0.492
2.704GluAsn: 2.704 ± 0.482
1.308GluPro: 1.308 ± 0.277
3.489GluGln: 3.489 ± 0.618
3.663GluArg: 3.663 ± 0.426
5.669GluSer: 5.669 ± 0.794
2.442GluThr: 2.442 ± 0.412
5.407GluVal: 5.407 ± 0.693
1.047GluTrp: 1.047 ± 0.343
4.012GluTyr: 4.012 ± 0.643
0.0GluXaa: 0.0 ± 0.0
Phe
1.919PheAla: 1.919 ± 0.389
0.262PheCys: 0.262 ± 0.113
3.925PheAsp: 3.925 ± 0.49
1.744PheGlu: 1.744 ± 0.329
0.523PhePhe: 0.523 ± 0.23
2.355PheGly: 2.355 ± 0.423
0.523PheHis: 0.523 ± 0.188
1.919PheIle: 1.919 ± 0.534
2.529PheLys: 2.529 ± 0.405
3.925PheLeu: 3.925 ± 0.493
1.308PheMet: 1.308 ± 0.309
2.442PheAsn: 2.442 ± 0.533
1.57PhePro: 1.57 ± 0.334
1.047PheGln: 1.047 ± 0.278
2.355PheArg: 2.355 ± 0.569
2.616PheSer: 2.616 ± 0.504
2.616PheThr: 2.616 ± 0.529
1.919PheVal: 1.919 ± 0.462
0.349PheTrp: 0.349 ± 0.145
2.093PheTyr: 2.093 ± 0.452
0.0PheXaa: 0.0 ± 0.0
Gly
5.843GlyAla: 5.843 ± 0.935
0.872GlyCys: 0.872 ± 0.231
5.058GlyAsp: 5.058 ± 0.561
4.884GlyGlu: 4.884 ± 0.696
2.965GlyPhe: 2.965 ± 0.408
4.622GlyGly: 4.622 ± 0.643
0.959GlyHis: 0.959 ± 0.29
4.622GlyIle: 4.622 ± 0.5
5.495GlyLys: 5.495 ± 0.789
7.239GlyLeu: 7.239 ± 0.815
1.57GlyMet: 1.57 ± 0.37
3.314GlyAsn: 3.314 ± 0.442
0.0GlyPro: 0.0 ± 0.0
2.616GlyGln: 2.616 ± 0.454
3.663GlyArg: 3.663 ± 0.428
4.71GlySer: 4.71 ± 0.822
4.361GlyThr: 4.361 ± 0.475
4.099GlyVal: 4.099 ± 0.515
0.872GlyTrp: 0.872 ± 0.32
3.401GlyTyr: 3.401 ± 0.481
0.0GlyXaa: 0.0 ± 0.0
His
1.047HisAla: 1.047 ± 0.333
0.349HisCys: 0.349 ± 0.163
0.785HisAsp: 0.785 ± 0.257
0.872HisGlu: 0.872 ± 0.29
0.785HisPhe: 0.785 ± 0.277
1.483HisGly: 1.483 ± 0.386
0.436HisHis: 0.436 ± 0.19
1.832HisIle: 1.832 ± 0.29
1.047HisLys: 1.047 ± 0.259
1.832HisLeu: 1.832 ± 0.425
0.174HisMet: 0.174 ± 0.124
1.134HisAsn: 1.134 ± 0.282
0.523HisPro: 0.523 ± 0.189
0.349HisGln: 0.349 ± 0.162
0.872HisArg: 0.872 ± 0.285
1.134HisSer: 1.134 ± 0.299
1.657HisThr: 1.657 ± 0.439
1.047HisVal: 1.047 ± 0.28
0.262HisTrp: 0.262 ± 0.121
0.785HisTyr: 0.785 ± 0.237
0.0HisXaa: 0.0 ± 0.0
Ile
3.663IleAla: 3.663 ± 0.466
0.349IleCys: 0.349 ± 0.217
3.314IleAsp: 3.314 ± 0.472
4.535IleGlu: 4.535 ± 0.569
1.57IlePhe: 1.57 ± 0.52
4.012IleGly: 4.012 ± 0.422
1.483IleHis: 1.483 ± 0.386
3.14IleIle: 3.14 ± 0.71
5.495IleLys: 5.495 ± 0.701
5.058IleLeu: 5.058 ± 0.566
1.483IleMet: 1.483 ± 0.358
2.878IleAsn: 2.878 ± 0.364
2.878IlePro: 2.878 ± 0.477
2.616IleGln: 2.616 ± 0.619
2.268IleArg: 2.268 ± 0.383
3.837IleSer: 3.837 ± 0.531
4.012IleThr: 4.012 ± 0.351
2.791IleVal: 2.791 ± 0.629
0.523IleTrp: 0.523 ± 0.178
2.093IleTyr: 2.093 ± 0.422
0.0IleXaa: 0.0 ± 0.0
Lys
7.413LysAla: 7.413 ± 0.872
0.349LysCys: 0.349 ± 0.169
3.663LysAsp: 3.663 ± 0.585
5.495LysGlu: 5.495 ± 0.773
2.529LysPhe: 2.529 ± 0.481
6.367LysGly: 6.367 ± 0.71
1.832LysHis: 1.832 ± 0.47
2.878LysIle: 2.878 ± 0.526
4.971LysLys: 4.971 ± 0.678
6.279LysLeu: 6.279 ± 0.799
1.657LysMet: 1.657 ± 0.435
3.053LysAsn: 3.053 ± 0.483
2.791LysPro: 2.791 ± 0.518
4.448LysGln: 4.448 ± 0.409
2.878LysArg: 2.878 ± 0.564
4.361LysSer: 4.361 ± 0.728
4.361LysThr: 4.361 ± 0.598
5.32LysVal: 5.32 ± 0.754
1.657LysTrp: 1.657 ± 0.404
3.75LysTyr: 3.75 ± 0.657
0.0LysXaa: 0.0 ± 0.0
Leu
6.105LeuAla: 6.105 ± 0.856
0.087LeuCys: 0.087 ± 0.082
5.931LeuAsp: 5.931 ± 0.514
7.239LeuGlu: 7.239 ± 0.93
1.919LeuPhe: 1.919 ± 0.46
5.756LeuGly: 5.756 ± 0.52
1.047LeuHis: 1.047 ± 0.31
4.448LeuIle: 4.448 ± 0.521
7.675LeuLys: 7.675 ± 0.817
7.326LeuLeu: 7.326 ± 0.921
2.442LeuMet: 2.442 ± 0.373
5.582LeuAsn: 5.582 ± 0.681
3.576LeuPro: 3.576 ± 0.578
4.274LeuGln: 4.274 ± 0.476
4.971LeuArg: 4.971 ± 0.63
5.407LeuSer: 5.407 ± 0.551
4.361LeuThr: 4.361 ± 0.574
6.279LeuVal: 6.279 ± 0.639
0.872LeuTrp: 0.872 ± 0.39
3.053LeuTyr: 3.053 ± 0.537
0.0LeuXaa: 0.0 ± 0.0
Met
1.57MetAla: 1.57 ± 0.378
0.349MetCys: 0.349 ± 0.174
1.483MetAsp: 1.483 ± 0.289
1.657MetGlu: 1.657 ± 0.427
0.872MetPhe: 0.872 ± 0.29
1.832MetGly: 1.832 ± 0.598
0.174MetHis: 0.174 ± 0.112
0.959MetIle: 0.959 ± 0.312
1.047MetLys: 1.047 ± 0.28
2.355MetLeu: 2.355 ± 0.37
0.174MetMet: 0.174 ± 0.113
0.698MetAsn: 0.698 ± 0.271
0.872MetPro: 0.872 ± 0.241
1.047MetGln: 1.047 ± 0.324
0.698MetArg: 0.698 ± 0.203
1.744MetSer: 1.744 ± 0.313
1.483MetThr: 1.483 ± 0.339
1.832MetVal: 1.832 ± 0.361
0.0MetTrp: 0.0 ± 0.0
0.436MetTyr: 0.436 ± 0.186
0.0MetXaa: 0.0 ± 0.0
Asn
3.489AsnAla: 3.489 ± 0.548
0.698AsnCys: 0.698 ± 0.238
3.14AsnAsp: 3.14 ± 0.516
3.227AsnGlu: 3.227 ± 0.477
1.919AsnPhe: 1.919 ± 0.358
4.099AsnGly: 4.099 ± 0.602
0.785AsnHis: 0.785 ± 0.219
3.576AsnIle: 3.576 ± 0.52
3.75AsnLys: 3.75 ± 0.616
4.622AsnLeu: 4.622 ± 0.592
0.611AsnMet: 0.611 ± 0.183
2.878AsnAsn: 2.878 ± 0.464
2.878AsnPro: 2.878 ± 0.342
1.919AsnGln: 1.919 ± 0.405
2.093AsnArg: 2.093 ± 0.483
3.837AsnSer: 3.837 ± 0.565
2.965AsnThr: 2.965 ± 0.491
3.227AsnVal: 3.227 ± 0.416
0.349AsnTrp: 0.349 ± 0.171
2.355AsnTyr: 2.355 ± 0.38
0.0AsnXaa: 0.0 ± 0.0
Pro
2.006ProAla: 2.006 ± 0.38
0.087ProCys: 0.087 ± 0.085
2.268ProAsp: 2.268 ± 0.422
2.616ProGlu: 2.616 ± 0.53
2.093ProPhe: 2.093 ± 0.467
0.262ProGly: 0.262 ± 0.169
0.872ProHis: 0.872 ± 0.292
1.832ProIle: 1.832 ± 0.381
2.355ProLys: 2.355 ± 0.595
2.616ProLeu: 2.616 ± 0.484
0.523ProMet: 0.523 ± 0.205
2.704ProAsn: 2.704 ± 0.399
1.134ProPro: 1.134 ± 0.543
2.093ProGln: 2.093 ± 0.431
0.959ProArg: 0.959 ± 0.294
2.878ProSer: 2.878 ± 0.468
2.355ProThr: 2.355 ± 0.391
1.919ProVal: 1.919 ± 0.425
0.087ProTrp: 0.087 ± 0.089
1.657ProTyr: 1.657 ± 0.36
0.0ProXaa: 0.0 ± 0.0
Gln
4.012GlnAla: 4.012 ± 0.456
0.436GlnCys: 0.436 ± 0.193
2.616GlnAsp: 2.616 ± 0.439
3.489GlnGlu: 3.489 ± 0.605
2.006GlnPhe: 2.006 ± 0.339
2.704GlnGly: 2.704 ± 0.376
0.698GlnHis: 0.698 ± 0.205
2.791GlnIle: 2.791 ± 0.384
2.442GlnLys: 2.442 ± 0.396
3.314GlnLeu: 3.314 ± 0.437
1.395GlnMet: 1.395 ± 0.309
1.221GlnAsn: 1.221 ± 0.356
1.221GlnPro: 1.221 ± 0.308
2.355GlnGln: 2.355 ± 0.385
2.093GlnArg: 2.093 ± 0.422
2.965GlnSer: 2.965 ± 0.447
2.18GlnThr: 2.18 ± 0.354
3.401GlnVal: 3.401 ± 0.529
0.436GlnTrp: 0.436 ± 0.219
0.872GlnTyr: 0.872 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
3.401ArgAla: 3.401 ± 0.638
0.611ArgCys: 0.611 ± 0.23
3.75ArgAsp: 3.75 ± 0.578
2.442ArgGlu: 2.442 ± 0.39
1.657ArgPhe: 1.657 ± 0.361
3.314ArgGly: 3.314 ± 0.766
1.221ArgHis: 1.221 ± 0.289
2.268ArgIle: 2.268 ± 0.494
3.576ArgLys: 3.576 ± 0.515
4.971ArgLeu: 4.971 ± 0.769
1.308ArgMet: 1.308 ± 0.266
3.053ArgAsn: 3.053 ± 0.544
1.483ArgPro: 1.483 ± 0.335
1.395ArgGln: 1.395 ± 0.417
1.657ArgArg: 1.657 ± 0.329
2.878ArgSer: 2.878 ± 0.496
2.616ArgThr: 2.616 ± 0.364
2.268ArgVal: 2.268 ± 0.45
0.349ArgTrp: 0.349 ± 0.191
1.221ArgTyr: 1.221 ± 0.253
0.0ArgXaa: 0.0 ± 0.0
Ser
4.971SerAla: 4.971 ± 0.652
0.523SerCys: 0.523 ± 0.258
4.186SerAsp: 4.186 ± 0.594
4.535SerGlu: 4.535 ± 0.596
2.878SerPhe: 2.878 ± 0.511
5.669SerGly: 5.669 ± 0.658
1.134SerHis: 1.134 ± 0.289
4.186SerIle: 4.186 ± 0.625
4.622SerLys: 4.622 ± 0.662
5.407SerLeu: 5.407 ± 0.785
1.308SerMet: 1.308 ± 0.374
2.965SerAsn: 2.965 ± 0.601
2.529SerPro: 2.529 ± 0.471
3.227SerGln: 3.227 ± 0.659
3.14SerArg: 3.14 ± 0.547
3.401SerSer: 3.401 ± 0.573
4.448SerThr: 4.448 ± 0.694
3.14SerVal: 3.14 ± 0.573
1.047SerTrp: 1.047 ± 0.366
2.18SerTyr: 2.18 ± 0.474
0.0SerXaa: 0.0 ± 0.0
Thr
4.274ThrAla: 4.274 ± 0.534
0.436ThrCys: 0.436 ± 0.192
2.965ThrAsp: 2.965 ± 0.443
3.401ThrGlu: 3.401 ± 0.423
2.529ThrPhe: 2.529 ± 0.407
6.018ThrGly: 6.018 ± 0.653
0.959ThrHis: 0.959 ± 0.244
3.576ThrIle: 3.576 ± 0.547
4.797ThrLys: 4.797 ± 0.605
6.105ThrLeu: 6.105 ± 0.833
0.959ThrMet: 0.959 ± 0.276
3.314ThrAsn: 3.314 ± 0.536
3.053ThrPro: 3.053 ± 0.413
2.442ThrGln: 2.442 ± 0.435
2.355ThrArg: 2.355 ± 0.446
3.576ThrSer: 3.576 ± 0.592
3.227ThrThr: 3.227 ± 0.532
4.099ThrVal: 4.099 ± 0.594
1.047ThrTrp: 1.047 ± 0.245
1.657ThrTyr: 1.657 ± 0.285
0.0ThrXaa: 0.0 ± 0.0
Val
5.146ValAla: 5.146 ± 0.637
0.436ValCys: 0.436 ± 0.183
3.75ValAsp: 3.75 ± 0.67
4.448ValGlu: 4.448 ± 0.711
2.006ValPhe: 2.006 ± 0.461
5.756ValGly: 5.756 ± 0.756
1.308ValHis: 1.308 ± 0.287
3.925ValIle: 3.925 ± 0.422
5.407ValLys: 5.407 ± 0.463
5.233ValLeu: 5.233 ± 0.744
0.698ValMet: 0.698 ± 0.212
3.314ValAsn: 3.314 ± 0.645
1.221ValPro: 1.221 ± 0.315
2.006ValGln: 2.006 ± 0.304
2.965ValArg: 2.965 ± 0.599
3.837ValSer: 3.837 ± 0.55
5.407ValThr: 5.407 ± 0.578
4.274ValVal: 4.274 ± 0.514
0.872ValTrp: 0.872 ± 0.28
1.483ValTyr: 1.483 ± 0.278
0.0ValXaa: 0.0 ± 0.0
Trp
0.698TrpAla: 0.698 ± 0.228
0.349TrpCys: 0.349 ± 0.155
0.785TrpAsp: 0.785 ± 0.238
0.611TrpGlu: 0.611 ± 0.209
0.785TrpPhe: 0.785 ± 0.261
1.134TrpGly: 1.134 ± 0.263
0.262TrpHis: 0.262 ± 0.166
0.785TrpIle: 0.785 ± 0.295
0.959TrpLys: 0.959 ± 0.25
1.308TrpLeu: 1.308 ± 0.345
0.087TrpMet: 0.087 ± 0.085
0.698TrpAsn: 0.698 ± 0.273
0.0TrpPro: 0.0 ± 0.0
0.349TrpGln: 0.349 ± 0.154
0.436TrpArg: 0.436 ± 0.167
0.698TrpSer: 0.698 ± 0.259
0.872TrpThr: 0.872 ± 0.297
0.785TrpVal: 0.785 ± 0.225
0.087TrpTrp: 0.087 ± 0.082
0.523TrpTyr: 0.523 ± 0.253
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.227TyrAla: 3.227 ± 0.558
0.349TyrCys: 0.349 ± 0.166
1.483TyrAsp: 1.483 ± 0.384
2.355TyrGlu: 2.355 ± 0.506
1.134TyrPhe: 1.134 ± 0.331
2.268TyrGly: 2.268 ± 0.512
0.698TyrHis: 0.698 ± 0.25
2.529TyrIle: 2.529 ± 0.609
3.314TyrLys: 3.314 ± 0.435
2.965TyrLeu: 2.965 ± 0.485
0.785TyrMet: 0.785 ± 0.243
2.268TyrAsn: 2.268 ± 0.346
1.483TyrPro: 1.483 ± 0.416
1.57TyrGln: 1.57 ± 0.49
1.657TyrArg: 1.657 ± 0.308
2.878TyrSer: 2.878 ± 0.487
2.442TyrThr: 2.442 ± 0.344
2.18TyrVal: 2.18 ± 0.462
0.349TyrTrp: 0.349 ± 0.175
1.134TyrTyr: 1.134 ± 0.327
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 43 proteins (11467 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski