Ruminococcus sp. AM36-2AA

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; unclassified Ruminococcus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A374DT64|A0A374DT64_9FIRM Uncharacterized protein OS=Ruminococcus sp. AM36-2AA OX=2293210 GN=DW846_02825 PE=4 SV=1
MM1 pKa = 7.37TKK3 pKa = 10.04SEE5 pKa = 4.24DD6 pKa = 3.82GSWKK10 pKa = 10.62VEE12 pKa = 3.89TSKK15 pKa = 10.86ISAGALNEE23 pKa = 3.84YY24 pKa = 9.59MIQQLLGCADD34 pKa = 3.9KK35 pKa = 11.22DD36 pKa = 4.11SLCFTPEE43 pKa = 3.75EE44 pKa = 4.05LQKK47 pKa = 11.35VLDD50 pKa = 4.17DD51 pKa = 3.81RR52 pKa = 11.84AAEE55 pKa = 4.06KK56 pKa = 10.47KK57 pKa = 10.28AALEE61 pKa = 3.99KK62 pKa = 10.94SLNSTNKK69 pKa = 9.52IPKK72 pKa = 8.99TSEE75 pKa = 3.53KK76 pKa = 10.86SDD78 pKa = 3.57DD79 pKa = 3.83KK80 pKa = 11.48KK81 pKa = 11.38SADD84 pKa = 3.42GSAKK88 pKa = 10.24QNSDD92 pKa = 3.14TTDD95 pKa = 2.84GNTADD100 pKa = 3.8TAGSGEE106 pKa = 4.31NTTQEE111 pKa = 4.22YY112 pKa = 8.79TPDD115 pKa = 3.49ASQDD119 pKa = 3.52EE120 pKa = 4.68TDD122 pKa = 4.21SSGEE126 pKa = 3.98DD127 pKa = 3.32TAGSDD132 pKa = 4.77GDD134 pKa = 3.9VSDD137 pKa = 5.82DD138 pKa = 3.7GSSDD142 pKa = 3.76DD143 pKa = 4.53GSGDD147 pKa = 4.3GDD149 pKa = 3.91SQDD152 pKa = 3.87TKK154 pKa = 11.06QCTITILCDD163 pKa = 3.57TILNNMDD170 pKa = 3.71NLTAGKK176 pKa = 9.25EE177 pKa = 4.19AYY179 pKa = 10.23VPANGVILDD188 pKa = 3.92TSTVEE193 pKa = 4.05FTEE196 pKa = 4.54GEE198 pKa = 4.5TVFEE202 pKa = 4.12VLQRR206 pKa = 11.84VCEE209 pKa = 3.96YY210 pKa = 11.29AGIQLEE216 pKa = 4.42YY217 pKa = 10.23SWTPMYY223 pKa = 10.7DD224 pKa = 2.53SYY226 pKa = 11.65YY227 pKa = 9.87IQGINNLYY235 pKa = 10.65EE236 pKa = 4.28FDD238 pKa = 5.09CGAQSGWMYY247 pKa = 10.85KK248 pKa = 9.92VNGWFPNYY256 pKa = 10.01GCSKK260 pKa = 9.31YY261 pKa = 9.2TLEE264 pKa = 6.03DD265 pKa = 3.52GDD267 pKa = 5.02DD268 pKa = 3.68IVWCFTCNGLGADD281 pKa = 4.27VGAEE285 pKa = 4.13YY286 pKa = 10.76NGEE289 pKa = 4.0

Molecular weight: 31.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A374D4P5|A0A374D4P5_9FIRM Uncharacterized protein OS=Ruminococcus sp. AM36-2AA OX=2293210 GN=DW846_16075 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.68GRR39 pKa = 11.84KK40 pKa = 8.28QLSAA44 pKa = 3.9

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3275

0

3275

1061070

22

3014

324.0

36.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.388 ± 0.042

1.535 ± 0.018

5.786 ± 0.038

7.682 ± 0.055

4.016 ± 0.029

7.214 ± 0.037

1.77 ± 0.022

7.125 ± 0.043

7.085 ± 0.042

8.711 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.132 ± 0.025

4.293 ± 0.028

3.332 ± 0.023

3.252 ± 0.023

4.375 ± 0.04

5.833 ± 0.043

5.639 ± 0.047

6.841 ± 0.037

0.914 ± 0.013

4.076 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski