Lachnospiraceae bacterium OF09-33XD

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3402 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A396QA61|A0A396QA61_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium OF09-33XD OX=2292273 GN=DXA96_19885 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.32KK3 pKa = 10.21VLAVTAAAAMGLSLTACSGGAASGSGSDD31 pKa = 3.58SSGASSASGATSVQGASAPSDD52 pKa = 3.49SSSVSGGTAAKK63 pKa = 8.62DD64 pKa = 3.36TYY66 pKa = 10.66KK67 pKa = 10.47IAVVKK72 pKa = 10.88QMDD75 pKa = 4.03HH76 pKa = 6.87ASLDD80 pKa = 4.11EE81 pKa = 4.03IAGAVTAEE89 pKa = 4.11LDD91 pKa = 3.78VIAEE95 pKa = 4.25EE96 pKa = 3.84NHH98 pKa = 4.88ITIEE102 pKa = 4.2YY103 pKa = 9.49EE104 pKa = 4.2VYY106 pKa = 10.46SGQGEE111 pKa = 4.28QTVLKK116 pKa = 10.63QIGDD120 pKa = 3.43QAIADD125 pKa = 4.12GVDD128 pKa = 3.74AIIPIATLAAQVMAVCAEE146 pKa = 4.08DD147 pKa = 3.44TQTPVIYY154 pKa = 10.2AAISDD159 pKa = 4.12PEE161 pKa = 4.08AAEE164 pKa = 4.07VTGIDD169 pKa = 4.25YY170 pKa = 8.61VTGTSDD176 pKa = 4.28ALNTDD181 pKa = 4.87FIIDD185 pKa = 3.72MMLKK189 pKa = 10.22QNPDD193 pKa = 3.22TKK195 pKa = 10.81KK196 pKa = 10.78VGLLYY201 pKa = 10.8SLSEE205 pKa = 4.43PNSATPVAEE214 pKa = 3.67ARR216 pKa = 11.84EE217 pKa = 4.1YY218 pKa = 11.27LDD220 pKa = 3.47SKK222 pKa = 10.62GVGYY226 pKa = 10.85VEE228 pKa = 4.18ATGNTNDD235 pKa = 3.75EE236 pKa = 4.47VIAAASVLVSEE247 pKa = 5.18GVDD250 pKa = 3.37AVFTPTDD257 pKa = 4.1NIVMAAEE264 pKa = 3.9MAIAGTFAEE273 pKa = 4.81AGIPHH278 pKa = 5.71YY279 pKa = 9.7TGADD283 pKa = 3.26SFVRR287 pKa = 11.84NGAFATCGVNYY298 pKa = 9.68TDD300 pKa = 5.79LGTKK304 pKa = 8.71TADD307 pKa = 3.62LAYY310 pKa = 10.36QAITDD315 pKa = 3.86GMGGMEE321 pKa = 5.77DD322 pKa = 4.46YY323 pKa = 11.42YY324 pKa = 11.73KK325 pKa = 9.96MDD327 pKa = 3.34GGIITVNTEE336 pKa = 3.55TAEE339 pKa = 4.16TLKK342 pKa = 10.81ADD344 pKa = 3.72YY345 pKa = 11.71SMFASMGEE353 pKa = 4.03LVEE356 pKa = 4.5VSTTEE361 pKa = 3.84EE362 pKa = 3.8

Molecular weight: 36.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A396QHA3|A0A396QHA3_9FIRM Aminotransferase class III-fold pyridoxal phosphate-dependent enzyme OS=Lachnospiraceae bacterium OF09-33XD OX=2292273 GN=DXA96_09700 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.48GRR39 pKa = 11.84KK40 pKa = 8.27EE41 pKa = 3.72LSAA44 pKa = 4.99

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3402

0

3402

997160

20

3886

293.1

32.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.086 ± 0.046

1.652 ± 0.019

5.375 ± 0.031

7.662 ± 0.045

3.842 ± 0.032

7.757 ± 0.04

1.729 ± 0.021

6.763 ± 0.034

5.795 ± 0.032

9.237 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.072 ± 0.024

3.878 ± 0.029

3.572 ± 0.026

3.45 ± 0.024

5.078 ± 0.037

5.982 ± 0.035

5.248 ± 0.041

6.874 ± 0.031

0.975 ± 0.014

3.975 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski