Cryobacterium sp. TmT2-59

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Cryobacterium; unclassified Cryobacterium

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3217 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R8XNH0|A0A4R8XNH0_9MICO Deoxyribodipyrimidine photolyase OS=Cryobacterium sp. TmT2-59 OX=1259264 GN=E3T24_16845 PE=4 SV=1
MM1 pKa = 7.74ADD3 pKa = 3.43KK4 pKa = 11.18TEE6 pKa = 3.95LQYY9 pKa = 10.44TAEE12 pKa = 4.41HH13 pKa = 6.27EE14 pKa = 4.38WVLVDD19 pKa = 4.19GDD21 pKa = 4.41TVTVGITAYY30 pKa = 10.41AADD33 pKa = 3.87KK34 pKa = 11.02LGDD37 pKa = 3.66VVFVEE42 pKa = 5.02LPAVGSDD49 pKa = 3.35VAGGSVVGEE58 pKa = 4.03IEE60 pKa = 4.16STKK63 pKa = 10.87SVGEE67 pKa = 3.78LFAPVTGTVAEE78 pKa = 4.37TNDD81 pKa = 3.48AVVDD85 pKa = 4.09SPEE88 pKa = 4.59LVNSDD93 pKa = 3.52PFGAGWLIKK102 pKa = 9.31VTYY105 pKa = 10.73AEE107 pKa = 4.7LPPLLSYY114 pKa = 11.32AEE116 pKa = 4.09YY117 pKa = 10.38SALVGEE123 pKa = 4.72

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R8Y1L0|A0A4R8Y1L0_9MICO Single-stranded DNA-binding protein OS=Cryobacterium sp. TmT2-59 OX=1259264 GN=E3T24_07550 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3217

0

3217

1037322

30

1975

322.5

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.181 ± 0.058

0.553 ± 0.01

5.804 ± 0.038

5.235 ± 0.037

3.228 ± 0.022

8.928 ± 0.039

1.966 ± 0.018

4.619 ± 0.029

2.263 ± 0.035

10.575 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.822 ± 0.017

2.253 ± 0.023

5.358 ± 0.033

2.916 ± 0.023

7.007 ± 0.04

5.939 ± 0.033

6.276 ± 0.038

8.711 ± 0.038

1.398 ± 0.018

1.968 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski