Amino acid dipepetide frequency for Solibacillus sp. R5-41

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.732AlaAla: 5.732 ± 0.098
0.579AlaCys: 0.579 ± 0.023
3.385AlaAsp: 3.385 ± 0.062
4.49AlaGlu: 4.49 ± 0.073
3.498AlaPhe: 3.498 ± 0.058
4.944AlaGly: 4.944 ± 0.076
1.378AlaHis: 1.378 ± 0.032
6.665AlaIle: 6.665 ± 0.084
4.855AlaLys: 4.855 ± 0.082
7.359AlaLeu: 7.359 ± 0.088
2.268AlaMet: 2.268 ± 0.05
3.238AlaAsn: 3.238 ± 0.06
2.081AlaPro: 2.081 ± 0.048
2.861AlaGln: 2.861 ± 0.056
2.588AlaArg: 2.588 ± 0.047
4.126AlaSer: 4.126 ± 0.067
4.248AlaThr: 4.248 ± 0.114
5.241AlaVal: 5.241 ± 0.08
0.55AlaTrp: 0.55 ± 0.021
2.389AlaTyr: 2.389 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.451CysAla: 0.451 ± 0.02
0.121CysCys: 0.121 ± 0.012
0.367CysAsp: 0.367 ± 0.019
0.501CysGlu: 0.501 ± 0.025
0.323CysPhe: 0.323 ± 0.017
0.636CysGly: 0.636 ± 0.024
0.177CysHis: 0.177 ± 0.013
0.596CysIle: 0.596 ± 0.023
0.365CysLys: 0.365 ± 0.02
0.626CysLeu: 0.626 ± 0.026
0.196CysMet: 0.196 ± 0.013
0.331CysAsn: 0.331 ± 0.019
0.324CysPro: 0.324 ± 0.019
0.223CysGln: 0.223 ± 0.014
0.252CysArg: 0.252 ± 0.014
0.484CysSer: 0.484 ± 0.02
0.421CysThr: 0.421 ± 0.021
0.42CysVal: 0.42 ± 0.019
0.081CysTrp: 0.081 ± 0.009
0.277CysTyr: 0.277 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.683AspAla: 3.683 ± 0.068
0.375AspCys: 0.375 ± 0.023
2.319AspAsp: 2.319 ± 0.057
4.492AspGlu: 4.492 ± 0.075
2.615AspPhe: 2.615 ± 0.05
3.379AspGly: 3.379 ± 0.062
0.929AspHis: 0.929 ± 0.027
3.966AspIle: 3.966 ± 0.06
2.954AspLys: 2.954 ± 0.055
4.6AspLeu: 4.6 ± 0.071
1.188AspMet: 1.188 ± 0.032
1.93AspAsn: 1.93 ± 0.045
1.646AspPro: 1.646 ± 0.036
1.62AspGln: 1.62 ± 0.038
1.836AspArg: 1.836 ± 0.039
2.475AspSer: 2.475 ± 0.048
2.409AspThr: 2.409 ± 0.047
3.957AspVal: 3.957 ± 0.066
0.567AspTrp: 0.567 ± 0.024
2.177AspTyr: 2.177 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
5.333GluAla: 5.333 ± 0.082
0.378GluCys: 0.378 ± 0.021
3.534GluAsp: 3.534 ± 0.064
6.275GluGlu: 6.275 ± 0.104
2.584GluPhe: 2.584 ± 0.054
4.065GluGly: 4.065 ± 0.064
1.514GluHis: 1.514 ± 0.034
5.805GluIle: 5.805 ± 0.082
6.132GluLys: 6.132 ± 0.072
7.108GluLeu: 7.108 ± 0.1
2.234GluMet: 2.234 ± 0.045
3.876GluAsn: 3.876 ± 0.065
1.954GluPro: 1.954 ± 0.045
4.35GluGln: 4.35 ± 0.077
3.253GluArg: 3.253 ± 0.054
3.387GluSer: 3.387 ± 0.06
3.769GluThr: 3.769 ± 0.068
5.17GluVal: 5.17 ± 0.075
0.825GluTrp: 0.825 ± 0.026
2.094GluTyr: 2.094 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
3.433PheAla: 3.433 ± 0.066
0.362PheCys: 0.362 ± 0.019
2.501PheAsp: 2.501 ± 0.052
3.347PheGlu: 3.347 ± 0.063
2.435PhePhe: 2.435 ± 0.061
3.343PheGly: 3.343 ± 0.06
0.942PheHis: 0.942 ± 0.03
4.414PheIle: 4.414 ± 0.083
2.539PheLys: 2.539 ± 0.048
4.493PheLeu: 4.493 ± 0.087
1.309PheMet: 1.309 ± 0.034
2.206PheAsn: 2.206 ± 0.047
1.56PhePro: 1.56 ± 0.041
1.641PheGln: 1.641 ± 0.042
1.464PheArg: 1.464 ± 0.035
3.264PheSer: 3.264 ± 0.069
2.855PheThr: 2.855 ± 0.051
3.477PheVal: 3.477 ± 0.064
0.492PheTrp: 0.492 ± 0.024
1.833PheTyr: 1.833 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
4.794GlyAla: 4.794 ± 0.119
0.578GlyCys: 0.578 ± 0.024
3.075GlyAsp: 3.075 ± 0.06
4.404GlyGlu: 4.404 ± 0.065
3.331GlyPhe: 3.331 ± 0.063
4.478GlyGly: 4.478 ± 0.114
1.355GlyHis: 1.355 ± 0.035
5.846GlyIle: 5.846 ± 0.092
4.512GlyLys: 4.512 ± 0.065
6.048GlyLeu: 6.048 ± 0.075
1.991GlyMet: 1.991 ± 0.044
2.683GlyAsn: 2.683 ± 0.052
1.478GlyPro: 1.478 ± 0.037
2.315GlyGln: 2.315 ± 0.05
2.316GlyArg: 2.316 ± 0.051
3.636GlySer: 3.636 ± 0.06
3.971GlyThr: 3.971 ± 0.112
4.696GlyVal: 4.696 ± 0.065
0.67GlyTrp: 0.67 ± 0.026
2.683GlyTyr: 2.683 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
1.322HisAla: 1.322 ± 0.034
0.23HisCys: 0.23 ± 0.016
0.955HisAsp: 0.955 ± 0.028
1.441HisGlu: 1.441 ± 0.039
1.141HisPhe: 1.141 ± 0.032
1.283HisGly: 1.283 ± 0.034
0.575HisHis: 0.575 ± 0.024
1.584HisIle: 1.584 ± 0.039
0.998HisLys: 0.998 ± 0.031
2.076HisLeu: 2.076 ± 0.044
0.483HisMet: 0.483 ± 0.022
0.808HisAsn: 0.808 ± 0.026
1.0HisPro: 1.0 ± 0.03
0.847HisGln: 0.847 ± 0.028
0.74HisArg: 0.74 ± 0.027
1.176HisSer: 1.176 ± 0.033
1.043HisThr: 1.043 ± 0.03
1.378HisVal: 1.378 ± 0.039
0.201HisTrp: 0.201 ± 0.015
0.941HisTyr: 0.941 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
6.739IleAla: 6.739 ± 0.073
0.687IleCys: 0.687 ± 0.023
4.429IleAsp: 4.429 ± 0.066
6.465IleGlu: 6.465 ± 0.085
3.821IlePhe: 3.821 ± 0.08
5.998IleGly: 5.998 ± 0.087
1.668IleHis: 1.668 ± 0.037
6.657IleIle: 6.657 ± 0.099
4.344IleLys: 4.344 ± 0.069
7.603IleLeu: 7.603 ± 0.093
1.867IleMet: 1.867 ± 0.038
3.423IleAsn: 3.423 ± 0.059
3.287IlePro: 3.287 ± 0.057
3.417IleGln: 3.417 ± 0.054
3.076IleArg: 3.076 ± 0.058
5.177IleSer: 5.177 ± 0.076
4.651IleThr: 4.651 ± 0.071
6.188IleVal: 6.188 ± 0.091
0.711IleTrp: 0.711 ± 0.032
2.593IleTyr: 2.593 ± 0.053
0.0IleXaa: 0.0 ± 0.0
Lys
4.318LysAla: 4.318 ± 0.073
0.341LysCys: 0.341 ± 0.019
3.444LysAsp: 3.444 ± 0.057
5.917LysGlu: 5.917 ± 0.083
2.232LysPhe: 2.232 ± 0.043
3.927LysGly: 3.927 ± 0.065
1.233LysHis: 1.233 ± 0.034
5.069LysIle: 5.069 ± 0.079
5.411LysLys: 5.411 ± 0.079
5.952LysLeu: 5.952 ± 0.085
2.374LysMet: 2.374 ± 0.049
3.601LysAsn: 3.601 ± 0.067
2.061LysPro: 2.061 ± 0.054
3.134LysGln: 3.134 ± 0.055
2.948LysArg: 2.948 ± 0.054
3.479LysSer: 3.479 ± 0.06
3.643LysThr: 3.643 ± 0.053
4.491LysVal: 4.491 ± 0.06
0.828LysTrp: 0.828 ± 0.026
2.291LysTyr: 2.291 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
7.624LeuAla: 7.624 ± 0.093
0.72LeuCys: 0.72 ± 0.026
4.629LeuAsp: 4.629 ± 0.07
6.328LeuGlu: 6.328 ± 0.087
4.999LeuPhe: 4.999 ± 0.096
5.983LeuGly: 5.983 ± 0.1
1.965LeuHis: 1.965 ± 0.046
7.523LeuIle: 7.523 ± 0.103
6.472LeuLys: 6.472 ± 0.082
10.144LeuLeu: 10.144 ± 0.143
2.588LeuMet: 2.588 ± 0.055
4.554LeuAsn: 4.554 ± 0.065
3.74LeuPro: 3.74 ± 0.062
4.171LeuGln: 4.171 ± 0.069
3.416LeuArg: 3.416 ± 0.063
6.226LeuSer: 6.226 ± 0.08
5.942LeuThr: 5.942 ± 0.084
6.396LeuVal: 6.396 ± 0.087
0.778LeuTrp: 0.778 ± 0.024
3.149LeuTyr: 3.149 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
1.986MetAla: 1.986 ± 0.046
0.142MetCys: 0.142 ± 0.011
1.486MetAsp: 1.486 ± 0.036
1.901MetGlu: 1.901 ± 0.041
1.11MetPhe: 1.11 ± 0.032
1.673MetGly: 1.673 ± 0.044
0.565MetHis: 0.565 ± 0.024
2.131MetIle: 2.131 ± 0.044
2.529MetLys: 2.529 ± 0.05
2.697MetLeu: 2.697 ± 0.05
0.881MetMet: 0.881 ± 0.029
1.627MetAsn: 1.627 ± 0.036
1.059MetPro: 1.059 ± 0.037
1.16MetGln: 1.16 ± 0.038
1.147MetArg: 1.147 ± 0.033
1.64MetSer: 1.64 ± 0.043
1.813MetThr: 1.813 ± 0.044
1.621MetVal: 1.621 ± 0.044
0.206MetTrp: 0.206 ± 0.012
0.865MetTyr: 0.865 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.162AsnAla: 3.162 ± 0.057
0.334AsnCys: 0.334 ± 0.019
2.339AsnAsp: 2.339 ± 0.048
3.936AsnGlu: 3.936 ± 0.062
1.931AsnPhe: 1.931 ± 0.04
3.386AsnGly: 3.386 ± 0.056
0.972AsnHis: 0.972 ± 0.033
3.667AsnIle: 3.667 ± 0.063
2.945AsnLys: 2.945 ± 0.061
4.111AsnLeu: 4.111 ± 0.068
1.283AsnMet: 1.283 ± 0.03
2.222AsnAsn: 2.222 ± 0.053
2.071AsnPro: 2.071 ± 0.042
1.687AsnGln: 1.687 ± 0.037
1.957AsnArg: 1.957 ± 0.042
2.573AsnSer: 2.573 ± 0.054
2.413AsnThr: 2.413 ± 0.045
3.308AsnVal: 3.308 ± 0.064
0.567AsnTrp: 0.567 ± 0.024
1.862AsnTyr: 1.862 ± 0.048
0.0AsnXaa: 0.0 ± 0.0
Pro
2.216ProAla: 2.216 ± 0.047
0.188ProCys: 0.188 ± 0.014
1.646ProAsp: 1.646 ± 0.039
2.585ProGlu: 2.585 ± 0.053
2.022ProPhe: 2.022 ± 0.048
1.901ProGly: 1.901 ± 0.048
0.753ProHis: 0.753 ± 0.025
3.081ProIle: 3.081 ± 0.057
2.147ProLys: 2.147 ± 0.047
3.279ProLeu: 3.279 ± 0.059
0.869ProMet: 0.869 ± 0.034
1.732ProAsn: 1.732 ± 0.04
0.869ProPro: 0.869 ± 0.033
1.202ProGln: 1.202 ± 0.036
0.938ProArg: 0.938 ± 0.026
2.11ProSer: 2.11 ± 0.041
2.147ProThr: 2.147 ± 0.049
2.542ProVal: 2.542 ± 0.053
0.301ProTrp: 0.301 ± 0.019
1.346ProTyr: 1.346 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.969GlnAla: 2.969 ± 0.059
0.208GlnCys: 0.208 ± 0.015
1.669GlnAsp: 1.669 ± 0.04
2.659GlnGlu: 2.659 ± 0.056
2.042GlnPhe: 2.042 ± 0.044
2.145GlnGly: 2.145 ± 0.048
0.924GlnHis: 0.924 ± 0.032
3.043GlnIle: 3.043 ± 0.055
2.804GlnLys: 2.804 ± 0.053
4.844GlnLeu: 4.844 ± 0.076
1.27GlnMet: 1.27 ± 0.033
1.836GlnAsn: 1.836 ± 0.045
1.272GlnPro: 1.272 ± 0.04
2.589GlnGln: 2.589 ± 0.062
1.537GlnArg: 1.537 ± 0.041
2.289GlnSer: 2.289 ± 0.041
2.138GlnThr: 2.138 ± 0.047
2.572GlnVal: 2.572 ± 0.045
0.431GlnTrp: 0.431 ± 0.021
1.523GlnTyr: 1.523 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.471ArgAla: 2.471 ± 0.049
0.238ArgCys: 0.238 ± 0.014
1.867ArgAsp: 1.867 ± 0.048
2.87ArgGlu: 2.87 ± 0.053
1.876ArgPhe: 1.876 ± 0.048
2.229ArgGly: 2.229 ± 0.047
0.756ArgHis: 0.756 ± 0.03
3.015ArgIle: 3.015 ± 0.058
2.767ArgLys: 2.767 ± 0.054
3.658ArgLeu: 3.658 ± 0.063
1.214ArgMet: 1.214 ± 0.034
1.844ArgAsn: 1.844 ± 0.038
1.16ArgPro: 1.16 ± 0.035
1.577ArgGln: 1.577 ± 0.046
1.552ArgArg: 1.552 ± 0.045
1.952ArgSer: 1.952 ± 0.042
1.939ArgThr: 1.939 ± 0.045
2.427ArgVal: 2.427 ± 0.039
0.379ArgTrp: 0.379 ± 0.018
1.409ArgTyr: 1.409 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
3.857SerAla: 3.857 ± 0.064
0.402SerCys: 0.402 ± 0.022
2.665SerAsp: 2.665 ± 0.05
3.693SerGlu: 3.693 ± 0.059
3.279SerPhe: 3.279 ± 0.05
3.93SerGly: 3.93 ± 0.061
1.086SerHis: 1.086 ± 0.027
5.277SerIle: 5.277 ± 0.076
3.891SerLys: 3.891 ± 0.051
5.773SerLeu: 5.773 ± 0.081
1.619SerMet: 1.619 ± 0.038
2.843SerAsn: 2.843 ± 0.056
1.972SerPro: 1.972 ± 0.044
1.888SerGln: 1.888 ± 0.046
1.983SerArg: 1.983 ± 0.038
3.563SerSer: 3.563 ± 0.066
3.33SerThr: 3.33 ± 0.053
3.997SerVal: 3.997 ± 0.064
0.577SerTrp: 0.577 ± 0.022
2.296SerTyr: 2.296 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
4.074ThrAla: 4.074 ± 0.061
0.333ThrCys: 0.333 ± 0.02
2.717ThrAsp: 2.717 ± 0.047
3.746ThrGlu: 3.746 ± 0.067
2.859ThrPhe: 2.859 ± 0.057
4.113ThrGly: 4.113 ± 0.213
1.145ThrHis: 1.145 ± 0.03
5.136ThrIle: 5.136 ± 0.064
3.551ThrLys: 3.551 ± 0.052
5.554ThrLeu: 5.554 ± 0.071
1.508ThrMet: 1.508 ± 0.035
2.786ThrAsn: 2.786 ± 0.048
2.244ThrPro: 2.244 ± 0.048
1.744ThrGln: 1.744 ± 0.037
1.827ThrArg: 1.827 ± 0.042
3.326ThrSer: 3.326 ± 0.062
3.37ThrThr: 3.37 ± 0.061
4.324ThrVal: 4.324 ± 0.073
0.504ThrTrp: 0.504 ± 0.023
2.108ThrTyr: 2.108 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
5.459ValAla: 5.459 ± 0.082
0.555ValCys: 0.555 ± 0.025
3.572ValAsp: 3.572 ± 0.059
5.157ValGlu: 5.157 ± 0.072
3.213ValPhe: 3.213 ± 0.06
4.523ValGly: 4.523 ± 0.074
1.338ValHis: 1.338 ± 0.036
5.764ValIle: 5.764 ± 0.084
4.612ValLys: 4.612 ± 0.074
6.878ValLeu: 6.878 ± 0.089
1.896ValMet: 1.896 ± 0.046
3.07ValAsn: 3.07 ± 0.059
2.51ValPro: 2.51 ± 0.05
2.68ValGln: 2.68 ± 0.047
2.575ValArg: 2.575 ± 0.056
4.255ValSer: 4.255 ± 0.069
4.355ValThr: 4.355 ± 0.068
5.212ValVal: 5.212 ± 0.077
0.569ValTrp: 0.569 ± 0.023
2.189ValTyr: 2.189 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
0.567TrpAla: 0.567 ± 0.022
0.073TrpCys: 0.073 ± 0.008
0.529TrpAsp: 0.529 ± 0.022
0.588TrpGlu: 0.588 ± 0.025
0.512TrpPhe: 0.512 ± 0.025
0.633TrpGly: 0.633 ± 0.022
0.238TrpHis: 0.238 ± 0.014
0.833TrpIle: 0.833 ± 0.031
0.658TrpLys: 0.658 ± 0.026
1.091TrpLeu: 1.091 ± 0.032
0.318TrpMet: 0.318 ± 0.017
0.512TrpAsn: 0.512 ± 0.02
0.226TrpPro: 0.226 ± 0.014
0.406TrpGln: 0.406 ± 0.021
0.359TrpArg: 0.359 ± 0.016
0.59TrpSer: 0.59 ± 0.026
0.531TrpThr: 0.531 ± 0.026
0.628TrpVal: 0.628 ± 0.027
0.123TrpTrp: 0.123 ± 0.011
0.347TrpTyr: 0.347 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.289TyrAla: 2.289 ± 0.049
0.328TyrCys: 0.328 ± 0.018
1.986TyrAsp: 1.986 ± 0.047
2.843TyrGlu: 2.843 ± 0.056
2.087TyrPhe: 2.087 ± 0.047
2.292TyrGly: 2.292 ± 0.049
0.709TyrHis: 0.709 ± 0.025
2.726TyrIle: 2.726 ± 0.05
2.211TyrLys: 2.211 ± 0.047
3.455TyrLeu: 3.455 ± 0.065
0.882TyrMet: 0.882 ± 0.03
1.604TyrAsn: 1.604 ± 0.036
1.361TyrPro: 1.361 ± 0.036
1.253TyrGln: 1.253 ± 0.037
1.434TyrArg: 1.434 ± 0.036
2.193TyrSer: 2.193 ± 0.046
1.983TyrThr: 1.983 ± 0.037
2.337TyrVal: 2.337 ± 0.039
0.399TyrTrp: 0.399 ± 0.018
1.551TyrTyr: 1.551 ± 0.041
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3944 proteins (1115748 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski