Epsilonproteobacteria bacterium SCGC AD-308-I21

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; unclassified Epsilonproteobacteria

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A318DDE4|A0A318DDE4_9PROT Uncharacterized protein OS=Epsilonproteobacteria bacterium SCGC AD-308-I21 OX=1449167 GN=BGW53_1201 PE=4 SV=1
MM1 pKa = 7.71AIIKK5 pKa = 9.97VDD7 pKa = 3.36EE8 pKa = 4.16VDD10 pKa = 3.98FQEE13 pKa = 4.29VLSNEE18 pKa = 4.08FEE20 pKa = 4.13KK21 pKa = 11.41GNIVILKK28 pKa = 8.95FISEE32 pKa = 4.29YY33 pKa = 10.92CDD35 pKa = 2.74ACMALGFEE43 pKa = 4.32LQEE46 pKa = 4.83LNGKK50 pKa = 8.74YY51 pKa = 10.5DD52 pKa = 3.36NVSILEE58 pKa = 4.03IDD60 pKa = 3.7CGEE63 pKa = 4.21SGEE66 pKa = 4.27LASIYY71 pKa = 10.85GVFEE75 pKa = 4.18VPTMVIYY82 pKa = 10.66EE83 pKa = 4.29NEE85 pKa = 4.21DD86 pKa = 3.28SVLWHH91 pKa = 5.74QEE93 pKa = 3.87GVVLAQDD100 pKa = 3.13IEE102 pKa = 4.67KK103 pKa = 10.29IINVV107 pKa = 3.6

Molecular weight: 12.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A318DI05|A0A318DI05_9PROT Outer membrane protein OmpA-like peptidoglycan-associated protein OS=Epsilonproteobacteria bacterium SCGC AD-308-I21 OX=1449167 GN=BGW53_10822 PE=4 SV=1
MM1 pKa = 7.33RR2 pKa = 11.84NKK4 pKa = 10.47YY5 pKa = 9.69IEE7 pKa = 4.38NFEE10 pKa = 4.2KK11 pKa = 10.88AQTAEE16 pKa = 3.84KK17 pKa = 9.47TLPEE21 pKa = 4.2FRR23 pKa = 11.84AGDD26 pKa = 3.88TLRR29 pKa = 11.84LAVAIKK35 pKa = 10.41EE36 pKa = 4.13GDD38 pKa = 3.33KK39 pKa = 10.95TRR41 pKa = 11.84VQAYY45 pKa = 9.48EE46 pKa = 4.3GVCISKK52 pKa = 10.37RR53 pKa = 11.84GQGTGQTITVRR64 pKa = 11.84KK65 pKa = 9.6IGANGIGIEE74 pKa = 4.54RR75 pKa = 11.84IFPIYY80 pKa = 10.06SDD82 pKa = 4.5SIQEE86 pKa = 3.67IKK88 pKa = 10.32VIRR91 pKa = 11.84RR92 pKa = 11.84GRR94 pKa = 11.84VRR96 pKa = 11.84RR97 pKa = 11.84AKK99 pKa = 10.5LFYY102 pKa = 10.94LRR104 pKa = 11.84DD105 pKa = 3.54LAGKK109 pKa = 9.24KK110 pKa = 10.21ARR112 pKa = 11.84IKK114 pKa = 9.79EE115 pKa = 4.1LRR117 pKa = 11.84RR118 pKa = 11.84KK119 pKa = 9.45

Molecular weight: 13.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

673

0

673

209116

31

1297

310.7

35.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.579 ± 0.107

0.778 ± 0.035

5.834 ± 0.063

7.185 ± 0.09

4.876 ± 0.084

5.607 ± 0.091

2.036 ± 0.041

8.596 ± 0.084

8.478 ± 0.104

9.792 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.693 ± 0.058

5.472 ± 0.09

2.814 ± 0.052

3.055 ± 0.054

3.224 ± 0.058

6.928 ± 0.078

5.155 ± 0.078

6.136 ± 0.074

0.731 ± 0.025

4.03 ± 0.073
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski