Venustampulla echinocandica

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Pleuroascaceae; Venustampulla

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10697 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370TXZ0|A0A370TXZ0_9HELO Uncharacterized protein OS=Venustampulla echinocandica OX=2656787 GN=BP5553_00357 PE=4 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.2NIYY6 pKa = 11.01DD7 pKa = 4.05EE8 pKa = 5.38IEE10 pKa = 4.56IEE12 pKa = 4.86DD13 pKa = 3.87MTYY16 pKa = 10.95DD17 pKa = 3.48AAIGIYY23 pKa = 9.7HH24 pKa = 6.0YY25 pKa = 9.67PCPCGDD31 pKa = 3.63RR32 pKa = 11.84FQIALADD39 pKa = 3.86LRR41 pKa = 11.84DD42 pKa = 3.79EE43 pKa = 4.51EE44 pKa = 6.24DD45 pKa = 3.22IAVCPSCSLMIRR57 pKa = 11.84VIFEE61 pKa = 3.84VDD63 pKa = 4.26DD64 pKa = 4.08LPKK67 pKa = 10.54PADD70 pKa = 3.75EE71 pKa = 4.32EE72 pKa = 4.34AKK74 pKa = 10.63EE75 pKa = 3.82PAAAVAVAAA84 pKa = 4.86

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370TUT5|A0A370TUT5_9HELO HET domain-containing protein OS=Venustampulla echinocandica OX=2656787 GN=BP5553_03637 PE=4 SV=1
MM1 pKa = 7.39GAGRR5 pKa = 11.84SPPVVRR11 pKa = 11.84KK12 pKa = 9.8VKK14 pKa = 10.38LRR16 pKa = 11.84LPHH19 pKa = 6.46FPQSVGGNGALASNGGQNSMGHH41 pKa = 6.15NPTPTPPAPTVGNRR55 pKa = 11.84GGGGGGRR62 pKa = 11.84GSMMGQNRR70 pKa = 11.84LAMGNGPNGVGSGNVMGRR88 pKa = 11.84NGVNGPPTGNPDD100 pKa = 3.27GGGSGMGSFGPPMRR114 pKa = 11.84IPSRR118 pKa = 11.84GGRR121 pKa = 11.84GGSRR125 pKa = 11.84GGSRR129 pKa = 11.84GGGRR133 pKa = 11.84GGGILARR140 pKa = 11.84GGGSRR145 pKa = 11.84RR146 pKa = 11.84VV147 pKa = 3.19

Molecular weight: 14.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10697

0

10697

5497940

52

13565

514.0

56.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.4 ± 0.018

1.212 ± 0.009

5.615 ± 0.015

6.189 ± 0.023

3.663 ± 0.014

6.985 ± 0.023

2.303 ± 0.009

5.186 ± 0.016

5.075 ± 0.023

8.834 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.171 ± 0.008

3.845 ± 0.012

6.145 ± 0.026

3.969 ± 0.017

5.878 ± 0.021

8.423 ± 0.027

5.999 ± 0.017

5.959 ± 0.019

1.414 ± 0.009

2.735 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski