Tortoise microvirus 59

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1FVZ6|A0A4V1FVZ6_9VIRU Major capsid protein OS=Tortoise microvirus 59 OX=2583164 PE=3 SV=1
MM1 pKa = 7.76SDD3 pKa = 4.67FIPIDD8 pKa = 3.67FFTDD12 pKa = 3.02DD13 pKa = 4.25GYY15 pKa = 11.87APVPVRR21 pKa = 11.84YY22 pKa = 8.71LASPEE27 pKa = 3.86TAEE30 pKa = 3.88VFNRR34 pKa = 11.84VMSMAPTFFPDD45 pKa = 4.22LPVDD49 pKa = 4.28ANPVIAEE56 pKa = 3.94QQYY59 pKa = 11.45AMALVTLAVNFYY71 pKa = 10.73FDD73 pKa = 4.57ALGLLSPPEE82 pKa = 4.25VAPSTPVGDD91 pKa = 3.93DD92 pKa = 3.19VLDD95 pKa = 3.77IKK97 pKa = 10.49EE98 pKa = 4.2SRR100 pKa = 11.84VV101 pKa = 3.25

Molecular weight: 11.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W6T5|A0A4P8W6T5_9VIRU Uncharacterized protein OS=Tortoise microvirus 59 OX=2583164 PE=4 SV=1
MM1 pKa = 7.48SCLSPVTIHH10 pKa = 6.46VNKK13 pKa = 10.0QSYY16 pKa = 7.94EE17 pKa = 4.27VPCRR21 pKa = 11.84QCLSCRR27 pKa = 11.84LDD29 pKa = 3.28YY30 pKa = 11.34QMYY33 pKa = 8.78LTFCVEE39 pKa = 3.77NEE41 pKa = 4.22LYY43 pKa = 10.68DD44 pKa = 3.74LYY46 pKa = 11.42KK47 pKa = 10.53RR48 pKa = 11.84GLGATFCTFTYY59 pKa = 10.88SDD61 pKa = 3.91VNLPSNGSLCKK72 pKa = 9.96SDD74 pKa = 3.76LQNLFKK80 pKa = 10.29TVRR83 pKa = 11.84TTAKK87 pKa = 10.17RR88 pKa = 11.84NGSLPPFKK96 pKa = 10.79YY97 pKa = 9.65IACGEE102 pKa = 4.18YY103 pKa = 10.04GDD105 pKa = 4.41KK106 pKa = 10.53FGRR109 pKa = 11.84PHH111 pKa = 5.0YY112 pKa = 10.21HH113 pKa = 6.23AVILGWSDD121 pKa = 3.85VICHH125 pKa = 5.67NMFRR129 pKa = 11.84KK130 pKa = 9.75YY131 pKa = 6.64WTHH134 pKa = 5.81GLSDD138 pKa = 4.08FGVLRR143 pKa = 11.84SGGVRR148 pKa = 11.84YY149 pKa = 9.49VLKK152 pKa = 10.73YY153 pKa = 7.99CTKK156 pKa = 10.14SVRR159 pKa = 11.84GTKK162 pKa = 10.19AEE164 pKa = 3.98EE165 pKa = 4.25MYY167 pKa = 10.64DD168 pKa = 3.53AQGLEE173 pKa = 4.27RR174 pKa = 11.84PFITHH179 pKa = 4.96SHH181 pKa = 6.54KK182 pKa = 10.43IGYY185 pKa = 8.85DD186 pKa = 3.31WLCRR190 pKa = 11.84NADD193 pKa = 4.24DD194 pKa = 4.84IVANNFTYY202 pKa = 10.66LNKK205 pKa = 10.15GVRR208 pKa = 11.84RR209 pKa = 11.84PIPKK213 pKa = 9.86YY214 pKa = 10.43FRR216 pKa = 11.84DD217 pKa = 3.46KK218 pKa = 11.04YY219 pKa = 11.44DD220 pKa = 3.2YY221 pKa = 10.93FKK223 pKa = 10.77TFNPMPAVDD232 pKa = 4.13FLRR235 pKa = 11.84RR236 pKa = 11.84EE237 pKa = 4.48GALHH241 pKa = 6.88NYY243 pKa = 8.63PDD245 pKa = 3.52YY246 pKa = 11.37RR247 pKa = 11.84EE248 pKa = 3.9WSKK251 pKa = 11.37VKK253 pKa = 10.42AYY255 pKa = 10.57NKK257 pKa = 10.13EE258 pKa = 3.58KK259 pKa = 11.02GLIQRR264 pKa = 11.84ARR266 pKa = 11.84QEE268 pKa = 4.66GIPVDD273 pKa = 3.93DD274 pKa = 5.32ANFIATPLPSPSLAPSLVTEE294 pKa = 4.22VLNRR298 pKa = 11.84PP299 pKa = 3.68

Molecular weight: 34.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1846

83

579

230.8

25.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.909 ± 1.287

1.517 ± 0.661

6.176 ± 0.484

4.55 ± 0.706

4.55 ± 0.544

5.796 ± 0.671

1.733 ± 0.257

4.28 ± 0.511

3.684 ± 0.989

9.534 ± 0.928

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.267

4.984 ± 0.431

5.85 ± 0.767

4.659 ± 1.714

5.525 ± 0.427

8.667 ± 0.925

6.23 ± 0.545

6.176 ± 0.778

0.65 ± 0.157

5.038 ± 0.798
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski