Amino acid dipepetide frequency for marine actinobacterium PHSC20C1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.305AlaAla: 17.305 ± 0.202	0.553AlaCys: 0.553 ± 0.027	7.1AlaAsp: 7.1 ± 0.105	7.557AlaGlu: 7.557 ± 0.106	3.851AlaPhe: 3.851 ± 0.074	10.524AlaGly: 10.524 ± 0.127	2.254AlaHis: 2.254 ± 0.055	7.191AlaIle: 7.191 ± 0.107	3.375AlaLys: 3.375 ± 0.071	13.212AlaLeu: 13.212 ± 0.185	2.535AlaMet: 2.535 ± 0.056	3.039AlaAsn: 3.039 ± 0.059	5.276AlaPro: 5.276 ± 0.096	3.827AlaGln: 3.827 ± 0.08	7.413AlaArg: 7.413 ± 0.093	7.946AlaSer: 7.946 ± 0.117	7.678AlaThr: 7.678 ± 0.16	10.392AlaVal: 10.392 ± 0.137	1.573AlaTrp: 1.573 ± 0.045	2.175AlaTyr: 2.175 ± 0.059	0.0AlaXaa: 0.0 ± 0.0
Cys	0.547CysAla: 0.547 ± 0.026	0.06CysCys: 0.06 ± 0.008	0.314CysAsp: 0.314 ± 0.021	0.295CysGlu: 0.295 ± 0.018	0.15CysPhe: 0.15 ± 0.014	0.504CysGly: 0.504 ± 0.027	0.108CysHis: 0.108 ± 0.012	0.223CysIle: 0.223 ± 0.017	0.067CysLys: 0.067 ± 0.009	0.404CysLeu: 0.404 ± 0.027	0.076CysMet: 0.076 ± 0.01	0.117CysAsn: 0.117 ± 0.013	0.281CysPro: 0.281 ± 0.018	0.141CysGln: 0.141 ± 0.013	0.268CysArg: 0.268 ± 0.018	0.361CysSer: 0.361 ± 0.02	0.336CysThr: 0.336 ± 0.023	0.422CysVal: 0.422 ± 0.023	0.06CysTrp: 0.06 ± 0.009	0.105CysTyr: 0.105 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.641AspAla: 7.641 ± 0.116	0.258AspCys: 0.258 ± 0.017	3.721AspAsp: 3.721 ± 0.095	4.058AspGlu: 4.058 ± 0.078	1.977AspPhe: 1.977 ± 0.054	5.073AspGly: 5.073 ± 0.096	1.148AspHis: 1.148 ± 0.038	2.625AspIle: 2.625 ± 0.057	1.274AspLys: 1.274 ± 0.039	5.636AspLeu: 5.636 ± 0.076	0.84AspMet: 0.84 ± 0.032	1.487AspAsn: 1.487 ± 0.047	3.427AspPro: 3.427 ± 0.066	1.601AspGln: 1.601 ± 0.045	3.759AspArg: 3.759 ± 0.071	3.783AspSer: 3.783 ± 0.072	2.905AspThr: 2.905 ± 0.067	5.099AspVal: 5.099 ± 0.081	0.834AspTrp: 0.834 ± 0.029	1.412AspTyr: 1.412 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	6.595GluAla: 6.595 ± 0.111	0.283GluCys: 0.283 ± 0.02	2.388GluAsp: 2.388 ± 0.057	2.976GluGlu: 2.976 ± 0.074	2.198GluPhe: 2.198 ± 0.057	3.782GluGly: 3.782 ± 0.078	1.41GluHis: 1.41 ± 0.041	3.263GluIle: 3.263 ± 0.069	1.708GluLys: 1.708 ± 0.054	7.441GluLeu: 7.441 ± 0.108	1.115GluMet: 1.115 ± 0.035	1.691GluAsn: 1.691 ± 0.049	2.834GluPro: 2.834 ± 0.068	2.232GluGln: 2.232 ± 0.055	4.451GluArg: 4.451 ± 0.081	3.747GluSer: 3.747 ± 0.064	3.223GluThr: 3.223 ± 0.058	4.394GluVal: 4.394 ± 0.084	0.881GluTrp: 0.881 ± 0.036	1.172GluTyr: 1.172 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	4.583PheAla: 4.583 ± 0.086	0.176PheCys: 0.176 ± 0.014	2.491PheAsp: 2.491 ± 0.053	2.09PheGlu: 2.09 ± 0.046	1.22PhePhe: 1.22 ± 0.043	3.635PheGly: 3.635 ± 0.074	0.551PheHis: 0.551 ± 0.026	1.609PheIle: 1.609 ± 0.048	0.605PheLys: 0.605 ± 0.03	2.862PheLeu: 2.862 ± 0.067	0.58PheMet: 0.58 ± 0.027	0.88PheAsn: 0.88 ± 0.031	1.379PhePro: 1.379 ± 0.047	0.794PheGln: 0.794 ± 0.033	1.644PheArg: 1.644 ± 0.049	2.379PheSer: 2.379 ± 0.058	2.384PheThr: 2.384 ± 0.059	2.99PheVal: 2.99 ± 0.061	0.482PheTrp: 0.482 ± 0.025	0.741PheTyr: 0.741 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	9.528GlyAla: 9.528 ± 0.117	0.527GlyCys: 0.527 ± 0.025	4.563GlyAsp: 4.563 ± 0.088	4.506GlyGlu: 4.506 ± 0.09	3.376GlyPhe: 3.376 ± 0.071	6.685GlyGly: 6.685 ± 0.111	1.75GlyHis: 1.75 ± 0.047	5.199GlyIle: 5.199 ± 0.074	2.662GlyLys: 2.662 ± 0.058	8.555GlyLeu: 8.555 ± 0.112	1.875GlyMet: 1.875 ± 0.056	2.174GlyAsn: 2.174 ± 0.076	3.076GlyPro: 3.076 ± 0.07	2.49GlyGln: 2.49 ± 0.054	4.906GlyArg: 4.906 ± 0.079	5.514GlySer: 5.514 ± 0.082	5.362GlyThr: 5.362 ± 0.123	7.255GlyVal: 7.255 ± 0.085	1.414GlyTrp: 1.414 ± 0.047	2.187GlyTyr: 2.187 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	2.118HisAla: 2.118 ± 0.05	0.12HisCys: 0.12 ± 0.012	1.233HisAsp: 1.233 ± 0.04	1.158HisGlu: 1.158 ± 0.04	0.617HisPhe: 0.617 ± 0.027	1.733HisGly: 1.733 ± 0.048	0.517HisHis: 0.517 ± 0.027	0.859HisIle: 0.859 ± 0.034	0.395HisLys: 0.395 ± 0.02	1.853HisLeu: 1.853 ± 0.052	0.383HisMet: 0.383 ± 0.021	0.487HisAsn: 0.487 ± 0.024	1.343HisPro: 1.343 ± 0.042	0.49HisGln: 0.49 ± 0.021	1.263HisArg: 1.263 ± 0.044	1.321HisSer: 1.321 ± 0.04	1.034HisThr: 1.034 ± 0.039	1.604HisVal: 1.604 ± 0.048	0.281HisTrp: 0.281 ± 0.02	0.47HisTyr: 0.47 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.92IleAla: 7.92 ± 0.112	0.274IleCys: 0.274 ± 0.018	3.88IleAsp: 3.88 ± 0.064	3.362IleGlu: 3.362 ± 0.064	1.617IlePhe: 1.617 ± 0.054	4.891IleGly: 4.891 ± 0.083	0.833IleHis: 0.833 ± 0.034	2.606IleIle: 2.606 ± 0.069	1.133IleLys: 1.133 ± 0.036	4.285IleLeu: 4.285 ± 0.091	0.815IleMet: 0.815 ± 0.032	1.42IleAsn: 1.42 ± 0.041	2.725IlePro: 2.725 ± 0.054	1.251IleGln: 1.251 ± 0.042	2.833IleArg: 2.833 ± 0.055	3.536IleSer: 3.536 ± 0.069	3.539IleThr: 3.539 ± 0.089	5.478IleVal: 5.478 ± 0.102	0.514IleTrp: 0.514 ± 0.023	0.923IleTyr: 0.923 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	2.897LysAla: 2.897 ± 0.064	0.079LysCys: 0.079 ± 0.01	1.358LysAsp: 1.358 ± 0.048	1.219LysGlu: 1.219 ± 0.046	0.812LysPhe: 0.812 ± 0.029	1.751LysGly: 1.751 ± 0.047	0.601LysHis: 0.601 ± 0.025	1.318LysIle: 1.318 ± 0.045	1.132LysLys: 1.132 ± 0.051	2.626LysLeu: 2.626 ± 0.062	0.568LysMet: 0.568 ± 0.027	0.874LysAsn: 0.874 ± 0.034	1.446LysPro: 1.446 ± 0.046	0.957LysGln: 0.957 ± 0.035	1.98LysArg: 1.98 ± 0.053	1.779LysSer: 1.779 ± 0.058	1.633LysThr: 1.633 ± 0.049	2.07LysVal: 2.07 ± 0.052	0.307LysTrp: 0.307 ± 0.018	0.59LysTyr: 0.59 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	13.48LeuAla: 13.48 ± 0.164	0.476LeuCys: 0.476 ± 0.023	6.387LeuAsp: 6.387 ± 0.096	5.475LeuGlu: 5.475 ± 0.097	3.029LeuPhe: 3.029 ± 0.077	8.872LeuGly: 8.872 ± 0.125	1.73LeuHis: 1.73 ± 0.057	5.388LeuIle: 5.388 ± 0.108	2.441LeuLys: 2.441 ± 0.056	9.797LeuLeu: 9.797 ± 0.152	1.794LeuMet: 1.794 ± 0.043	2.551LeuAsn: 2.551 ± 0.051	5.218LeuPro: 5.218 ± 0.088	2.376LeuGln: 2.376 ± 0.052	6.309LeuArg: 6.309 ± 0.103	7.141LeuSer: 7.141 ± 0.098	6.485LeuThr: 6.485 ± 0.095	8.876LeuVal: 8.876 ± 0.122	1.193LeuTrp: 1.193 ± 0.036	1.76LeuTyr: 1.76 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.227MetAla: 2.227 ± 0.057	0.083MetCys: 0.083 ± 0.009	0.816MetAsp: 0.816 ± 0.033	0.746MetGlu: 0.746 ± 0.031	0.638MetPhe: 0.638 ± 0.027	1.391MetGly: 1.391 ± 0.047	0.388MetHis: 0.388 ± 0.02	1.111MetIle: 1.111 ± 0.039	0.587MetLys: 0.587 ± 0.029	2.025MetLeu: 2.025 ± 0.054	0.345MetMet: 0.345 ± 0.022	0.653MetAsn: 0.653 ± 0.026	1.122MetPro: 1.122 ± 0.037	0.523MetGln: 0.523 ± 0.021	1.316MetArg: 1.316 ± 0.039	1.536MetSer: 1.536 ± 0.042	1.734MetThr: 1.734 ± 0.044	1.536MetVal: 1.536 ± 0.04	0.214MetTrp: 0.214 ± 0.016	0.308MetTyr: 0.308 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.967AsnAla: 2.967 ± 0.066	0.154AsnCys: 0.154 ± 0.014	1.646AsnAsp: 1.646 ± 0.051	1.485AsnGlu: 1.485 ± 0.047	0.985AsnPhe: 0.985 ± 0.034	2.387AsnGly: 2.387 ± 0.069	0.514AsnHis: 0.514 ± 0.024	1.223AsnIle: 1.223 ± 0.041	0.684AsnLys: 0.684 ± 0.028	2.444AsnLeu: 2.444 ± 0.058	0.506AsnMet: 0.506 ± 0.022	0.794AsnAsn: 0.794 ± 0.035	1.944AsnPro: 1.944 ± 0.049	0.886AsnGln: 0.886 ± 0.029	1.627AsnArg: 1.627 ± 0.045	1.723AsnSer: 1.723 ± 0.049	1.607AsnThr: 1.607 ± 0.096	2.205AsnVal: 2.205 ± 0.074	0.401AsnTrp: 0.401 ± 0.023	0.678AsnTyr: 0.678 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	5.905ProAla: 5.905 ± 0.097	0.184ProCys: 0.184 ± 0.015	3.161ProAsp: 3.161 ± 0.065	3.668ProGlu: 3.668 ± 0.072	1.621ProPhe: 1.621 ± 0.041	4.072ProGly: 4.072 ± 0.112	1.034ProHis: 1.034 ± 0.033	2.413ProIle: 2.413 ± 0.059	1.263ProLys: 1.263 ± 0.039	4.434ProLeu: 4.434 ± 0.075	0.907ProMet: 0.907 ± 0.037	1.379ProAsn: 1.379 ± 0.05	1.667ProPro: 1.667 ± 0.051	1.547ProGln: 1.547 ± 0.042	2.727ProArg: 2.727 ± 0.065	3.258ProSer: 3.258 ± 0.068	3.324ProThr: 3.324 ± 0.073	4.184ProVal: 4.184 ± 0.076	0.734ProTrp: 0.734 ± 0.034	1.012ProTyr: 1.012 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	3.337GlnAla: 3.337 ± 0.061	0.126GlnCys: 0.126 ± 0.013	1.144GlnAsp: 1.144 ± 0.038	1.284GlnGlu: 1.284 ± 0.037	1.02GlnPhe: 1.02 ± 0.038	1.943GlnGly: 1.943 ± 0.05	0.659GlnHis: 0.659 ± 0.033	1.603GlnIle: 1.603 ± 0.044	0.81GlnLys: 0.81 ± 0.027	3.642GlnLeu: 3.642 ± 0.072	0.614GlnMet: 0.614 ± 0.027	0.74GlnAsn: 0.74 ± 0.03	1.461GlnPro: 1.461 ± 0.043	1.251GlnGln: 1.251 ± 0.041	2.296GlnArg: 2.296 ± 0.063	1.959GlnSer: 1.959 ± 0.057	1.559GlnThr: 1.559 ± 0.044	2.343GlnVal: 2.343 ± 0.054	0.554GlnTrp: 0.554 ± 0.025	0.689GlnTyr: 0.689 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	7.264ArgAla: 7.264 ± 0.107	0.269ArgCys: 0.269 ± 0.018	3.733ArgAsp: 3.733 ± 0.076	4.102ArgGlu: 4.102 ± 0.077	2.178ArgPhe: 2.178 ± 0.047	4.648ArgGly: 4.648 ± 0.073	1.293ArgHis: 1.293 ± 0.04	3.41ArgIle: 3.41 ± 0.069	1.727ArgLys: 1.727 ± 0.058	6.046ArgLeu: 6.046 ± 0.092	1.595ArgMet: 1.595 ± 0.042	1.639ArgAsn: 1.639 ± 0.052	2.723ArgPro: 2.723 ± 0.07	1.908ArgGln: 1.908 ± 0.048	4.411ArgArg: 4.411 ± 0.092	4.022ArgSer: 4.022 ± 0.085	3.732ArgThr: 3.732 ± 0.071	5.181ArgVal: 5.181 ± 0.099	0.973ArgTrp: 0.973 ± 0.036	1.458ArgTyr: 1.458 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	8.106SerAla: 8.106 ± 0.108	0.289SerCys: 0.289 ± 0.021	3.763SerAsp: 3.763 ± 0.072	3.622SerGlu: 3.622 ± 0.074	2.322SerPhe: 2.322 ± 0.054	6.421SerGly: 6.421 ± 0.135	1.224SerHis: 1.224 ± 0.037	3.53SerIle: 3.53 ± 0.061	1.731SerLys: 1.731 ± 0.051	6.407SerLeu: 6.407 ± 0.089	1.381SerMet: 1.381 ± 0.037	1.811SerAsn: 1.811 ± 0.051	3.21SerPro: 3.21 ± 0.064	1.768SerGln: 1.768 ± 0.04	4.019SerArg: 4.019 ± 0.081	4.648SerSer: 4.648 ± 0.093	4.393SerThr: 4.393 ± 0.089	5.568SerVal: 5.568 ± 0.101	0.964SerTrp: 0.964 ± 0.034	1.424SerTyr: 1.424 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	7.663ThrAla: 7.663 ± 0.179	0.273ThrCys: 0.273 ± 0.016	3.532ThrAsp: 3.532 ± 0.07	3.536ThrGlu: 3.536 ± 0.065	2.165ThrPhe: 2.165 ± 0.053	5.539ThrGly: 5.539 ± 0.109	1.081ThrHis: 1.081 ± 0.037	3.699ThrIle: 3.699 ± 0.069	1.617ThrLys: 1.617 ± 0.054	6.202ThrLeu: 6.202 ± 0.104	1.068ThrMet: 1.068 ± 0.04	1.691ThrAsn: 1.691 ± 0.064	3.624ThrPro: 3.624 ± 0.083	1.684ThrGln: 1.684 ± 0.055	3.4ThrArg: 3.4 ± 0.061	3.843ThrSer: 3.843 ± 0.08	4.077ThrThr: 4.077 ± 0.088	6.019ThrVal: 6.019 ± 0.136	0.815ThrTrp: 0.815 ± 0.049	1.243ThrTyr: 1.243 ± 0.07	0.0ThrXaa: 0.0 ± 0.0
Val	11.027ValAla: 11.027 ± 0.137	0.429ValCys: 0.429 ± 0.021	5.348ValAsp: 5.348 ± 0.083	4.856ValGlu: 4.856 ± 0.084	2.886ValPhe: 2.886 ± 0.069	6.903ValGly: 6.903 ± 0.089	1.595ValHis: 1.595 ± 0.046	5.015ValIle: 5.015 ± 0.091	1.972ValLys: 1.972 ± 0.059	8.993ValLeu: 8.993 ± 0.131	1.61ValMet: 1.61 ± 0.052	2.314ValAsn: 2.314 ± 0.064	4.041ValPro: 4.041 ± 0.068	2.152ValGln: 2.152 ± 0.051	5.259ValArg: 5.259 ± 0.073	5.743ValSer: 5.743 ± 0.093	5.743ValThr: 5.743 ± 0.18	8.233ValVal: 8.233 ± 0.131	1.072ValTrp: 1.072 ± 0.037	1.435ValTyr: 1.435 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.428TrpAla: 1.428 ± 0.043	0.092TrpCys: 0.092 ± 0.011	0.666TrpAsp: 0.666 ± 0.029	0.585TrpGlu: 0.585 ± 0.028	0.545TrpPhe: 0.545 ± 0.026	1.001TrpGly: 1.001 ± 0.036	0.297TrpHis: 0.297 ± 0.018	0.719TrpIle: 0.719 ± 0.03	0.385TrpLys: 0.385 ± 0.021	1.682TrpLeu: 1.682 ± 0.052	0.381TrpMet: 0.381 ± 0.022	0.527TrpAsn: 0.527 ± 0.026	0.688TrpPro: 0.688 ± 0.053	0.572TrpGln: 0.572 ± 0.024	0.935TrpArg: 0.935 ± 0.034	0.924TrpSer: 0.924 ± 0.035	0.814TrpThr: 0.814 ± 0.034	1.078TrpVal: 1.078 ± 0.036	0.352TrpTrp: 0.352 ± 0.028	0.271TrpTyr: 0.271 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.239TyrAla: 2.239 ± 0.051	0.134TyrCys: 0.134 ± 0.012	1.313TyrAsp: 1.313 ± 0.049	1.206TyrGlu: 1.206 ± 0.04	0.87TyrPhe: 0.87 ± 0.032	1.849TyrGly: 1.849 ± 0.052	0.291TyrHis: 0.291 ± 0.02	0.833TyrIle: 0.833 ± 0.034	0.429TyrLys: 0.429 ± 0.025	2.231TyrLeu: 2.231 ± 0.057	0.309TyrMet: 0.309 ± 0.02	0.588TyrAsn: 0.588 ± 0.03	1.01TyrPro: 1.01 ± 0.033	0.587TyrGln: 0.587 ± 0.027	1.492TyrArg: 1.492 ± 0.041	1.439TyrSer: 1.439 ± 0.035	1.21TyrThr: 1.21 ± 0.056	1.697TyrVal: 1.697 ± 0.047	0.325TyrTrp: 0.325 ± 0.019	0.49TyrTyr: 0.49 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2668 proteins (851365 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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