Paenibacillus polymyxa (strain SC2) (Bacillus polymyxa)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; Paenibacillus polymyxa

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5377 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3E5U8|E3E5U8_PAEPS LacI family transcriptional regulator OS=Paenibacillus polymyxa (strain SC2) OX=886882 GN=purR7 PE=4 SV=1
MM1 pKa = 7.81PIIKK5 pKa = 9.61PVYY8 pKa = 8.21TAVATAPVATGGAISTTVTPAVTRR32 pKa = 11.84FFATITAGMIGATTTTIPAASFVDD56 pKa = 4.31DD57 pKa = 3.98ADD59 pKa = 4.13APVVALPTLTATDD72 pKa = 3.45SANYY76 pKa = 8.96YY77 pKa = 9.77INGVIQQSSLFTLTTASLVIASVDD101 pKa = 3.18ITPGVPAVIEE111 pKa = 3.95ISDD114 pKa = 3.71FSGTTSTITTQPTISAPTVTIITT137 pKa = 3.99

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3ECA4|E3ECA4_PAEPS UPF0291 protein PPSC2_01295 OS=Paenibacillus polymyxa (strain SC2) OX=886882 GN=M1-396 PE=3 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.72RR12 pKa = 11.84KK13 pKa = 8.96KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5377

0

5377

1702113

26

14083

316.6

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.667 ± 0.036

0.796 ± 0.012

5.142 ± 0.025

6.849 ± 0.048

4.059 ± 0.026

7.114 ± 0.03

2.294 ± 0.021

6.793 ± 0.038

5.588 ± 0.044

10.034 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.019

3.995 ± 0.026

3.919 ± 0.024

4.201 ± 0.031

4.916 ± 0.029

6.403 ± 0.028

5.539 ± 0.032

7.071 ± 0.026

1.231 ± 0.013

3.57 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski