Ralstonia phage RS-PII-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Okabevirinae; Sukuvirus; Ralstonia virus RSPII1

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L7DQE6|A0A1L7DQE6_9CAUD DNA packaging protein OS=Ralstonia phage RS-PII-1 OX=1932892 PE=4 SV=1
MM1 pKa = 7.31SRR3 pKa = 11.84DD4 pKa = 3.41ISNMDD9 pKa = 4.44DD10 pKa = 3.75IIDD13 pKa = 3.53VRR15 pKa = 11.84QVIEE19 pKa = 3.99RR20 pKa = 11.84VEE22 pKa = 3.93EE23 pKa = 3.88LRR25 pKa = 11.84EE26 pKa = 3.72QRR28 pKa = 11.84TPRR31 pKa = 11.84FIVGYY36 pKa = 9.4NLAGYY41 pKa = 8.55MPDD44 pKa = 3.59ADD46 pKa = 4.1PGEE49 pKa = 4.31FGDD52 pKa = 3.63WGDD55 pKa = 3.73AQRR58 pKa = 11.84EE59 pKa = 4.23LIDD62 pKa = 3.14ILKK65 pKa = 10.32RR66 pKa = 11.84FEE68 pKa = 4.5DD69 pKa = 4.0EE70 pKa = 4.01EE71 pKa = 4.94GIPEE75 pKa = 4.25GDD77 pKa = 3.19AAEE80 pKa = 4.3YY81 pKa = 10.28CHH83 pKa = 6.87AAEE86 pKa = 5.94DD87 pKa = 3.85VNLWSEE93 pKa = 4.2IDD95 pKa = 4.92DD96 pKa = 4.4GDD98 pKa = 4.55DD99 pKa = 4.33DD100 pKa = 5.08LVIHH104 pKa = 7.02AGLYY108 pKa = 9.9AFWIRR113 pKa = 11.84RR114 pKa = 11.84DD115 pKa = 3.22GFMGLDD121 pKa = 3.1SDD123 pKa = 4.91EE124 pKa = 4.4EE125 pKa = 4.48NEE127 pKa = 4.57LEE129 pKa = 4.64LLEE132 pKa = 4.88EE133 pKa = 4.62LLSDD137 pKa = 4.12LAGYY141 pKa = 10.22GGDD144 pKa = 4.19HH145 pKa = 5.57QWEE148 pKa = 4.53GDD150 pKa = 3.72WYY152 pKa = 9.21PVTLIRR158 pKa = 11.84DD159 pKa = 4.0SYY161 pKa = 9.16FQTYY165 pKa = 8.68AQEE168 pKa = 3.92FAEE171 pKa = 5.2DD172 pKa = 3.32IGAVEE177 pKa = 6.19HH178 pKa = 7.27DD179 pKa = 4.01AQWPNNCIDD188 pKa = 3.37WEE190 pKa = 4.21QAARR194 pKa = 11.84EE195 pKa = 4.03LRR197 pKa = 11.84MDD199 pKa = 3.71YY200 pKa = 11.11SSVDD204 pKa = 4.12FDD206 pKa = 5.67GEE208 pKa = 4.8TYY210 pKa = 9.79WYY212 pKa = 10.48RR213 pKa = 3.59

Molecular weight: 24.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L7DQN7|A0A1L7DQN7_9CAUD Uncharacterized protein OS=Ralstonia phage RS-PII-1 OX=1932892 PE=4 SV=1
MM1 pKa = 7.83SIEE4 pKa = 4.43EE5 pKa = 4.23VNQLLRR11 pKa = 11.84DD12 pKa = 3.61MAVPHH17 pKa = 6.04MLEE20 pKa = 3.59TAYY23 pKa = 10.73LRR25 pKa = 11.84NIWRR29 pKa = 11.84KK30 pKa = 8.66PFYY33 pKa = 9.53GTAFGVRR40 pKa = 11.84RR41 pKa = 11.84SRR43 pKa = 3.65

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

12847

43

1689

279.3

30.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.47 ± 0.603

0.833 ± 0.131

6.165 ± 0.213

5.916 ± 0.351

3.293 ± 0.198

7.971 ± 0.339

2.032 ± 0.147

4.203 ± 0.197

4.873 ± 0.308

8.36 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.177

3.892 ± 0.251

4.445 ± 0.233

4.382 ± 0.284

6.157 ± 0.378

5.402 ± 0.311

5.869 ± 0.333

6.624 ± 0.32

1.565 ± 0.139

2.958 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski