Amino acid dipepetide frequency for Synechococcus phage S-B68

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.031AlaAla: 8.031 ± 0.869
0.571AlaCys: 0.571 ± 0.103
5.886AlaAsp: 5.886 ± 0.742
5.61AlaGlu: 5.61 ± 0.49
2.913AlaPhe: 2.913 ± 0.27
7.697AlaGly: 7.697 ± 0.703
1.24AlaHis: 1.24 ± 0.146
3.898AlaIle: 3.898 ± 0.248
3.976AlaLys: 3.976 ± 0.507
6.378AlaLeu: 6.378 ± 0.377
1.417AlaMet: 1.417 ± 0.195
3.484AlaAsn: 3.484 ± 0.446
3.642AlaPro: 3.642 ± 0.351
3.169AlaGln: 3.169 ± 0.455
3.169AlaArg: 3.169 ± 0.273
4.901AlaSer: 4.901 ± 0.342
5.571AlaThr: 5.571 ± 0.557
4.567AlaVal: 4.567 ± 0.315
1.161AlaTrp: 1.161 ± 0.206
2.638AlaTyr: 2.638 ± 0.275
0.0AlaXaa: 0.0 ± 0.0
Cys
0.591CysAla: 0.591 ± 0.127
0.059CysCys: 0.059 ± 0.038
0.61CysAsp: 0.61 ± 0.12
0.807CysGlu: 0.807 ± 0.127
0.394CysPhe: 0.394 ± 0.125
0.492CysGly: 0.492 ± 0.128
0.256CysHis: 0.256 ± 0.078
0.433CysIle: 0.433 ± 0.099
0.433CysLys: 0.433 ± 0.094
0.512CysLeu: 0.512 ± 0.119
0.118CysMet: 0.118 ± 0.045
0.394CysAsn: 0.394 ± 0.104
0.512CysPro: 0.512 ± 0.131
0.394CysGln: 0.394 ± 0.091
0.354CysArg: 0.354 ± 0.104
0.61CysSer: 0.61 ± 0.105
0.394CysThr: 0.394 ± 0.085
0.669CysVal: 0.669 ± 0.12
0.217CysTrp: 0.217 ± 0.07
0.413CysTyr: 0.413 ± 0.09
0.0CysXaa: 0.0 ± 0.0
Asp
6.535AspAla: 6.535 ± 1.069
0.472AspCys: 0.472 ± 0.09
4.468AspAsp: 4.468 ± 0.5
4.016AspGlu: 4.016 ± 0.403
2.776AspPhe: 2.776 ± 0.245
6.378AspGly: 6.378 ± 0.559
0.945AspHis: 0.945 ± 0.146
3.917AspIle: 3.917 ± 0.348
2.992AspLys: 2.992 ± 0.345
6.614AspLeu: 6.614 ± 0.467
1.398AspMet: 1.398 ± 0.194
3.307AspAsn: 3.307 ± 0.339
3.209AspPro: 3.209 ± 0.294
2.224AspGln: 2.224 ± 0.232
2.461AspArg: 2.461 ± 0.297
3.937AspSer: 3.937 ± 0.335
4.488AspThr: 4.488 ± 0.603
4.547AspVal: 4.547 ± 0.415
1.024AspTrp: 1.024 ± 0.147
2.795AspTyr: 2.795 ± 0.258
0.0AspXaa: 0.0 ± 0.0
Glu
4.921GluAla: 4.921 ± 0.342
0.689GluCys: 0.689 ± 0.133
4.291GluAsp: 4.291 ± 0.426
5.649GluGlu: 5.649 ± 0.673
2.638GluPhe: 2.638 ± 0.3
4.429GluGly: 4.429 ± 0.361
1.063GluHis: 1.063 ± 0.177
4.055GluIle: 4.055 ± 0.264
3.563GluLys: 3.563 ± 0.416
5.984GluLeu: 5.984 ± 0.447
1.713GluMet: 1.713 ± 0.219
2.913GluAsn: 2.913 ± 0.306
1.909GluPro: 1.909 ± 0.269
2.598GluGln: 2.598 ± 0.289
3.11GluArg: 3.11 ± 0.347
3.287GluSer: 3.287 ± 0.265
3.346GluThr: 3.346 ± 0.302
5.256GluVal: 5.256 ± 0.498
0.925GluTrp: 0.925 ± 0.147
2.716GluTyr: 2.716 ± 0.291
0.0GluXaa: 0.0 ± 0.0
Phe
2.854PheAla: 2.854 ± 0.285
0.433PheCys: 0.433 ± 0.098
3.209PheAsp: 3.209 ± 0.311
2.323PheGlu: 2.323 ± 0.271
1.614PhePhe: 1.614 ± 0.21
2.913PheGly: 2.913 ± 0.354
0.866PheHis: 0.866 ± 0.15
2.126PheIle: 2.126 ± 0.243
2.283PheLys: 2.283 ± 0.324
3.012PheLeu: 3.012 ± 0.261
0.945PheMet: 0.945 ± 0.154
2.067PheAsn: 2.067 ± 0.255
1.358PhePro: 1.358 ± 0.166
1.339PheGln: 1.339 ± 0.156
1.811PheArg: 1.811 ± 0.248
3.051PheSer: 3.051 ± 0.298
3.327PheThr: 3.327 ± 0.324
2.657PheVal: 2.657 ± 0.209
0.472PheTrp: 0.472 ± 0.123
1.594PheTyr: 1.594 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
6.693GlyAla: 6.693 ± 0.71
0.374GlyCys: 0.374 ± 0.09
6.181GlyAsp: 6.181 ± 0.621
4.862GlyGlu: 4.862 ± 0.358
3.346GlyPhe: 3.346 ± 0.557
6.594GlyGly: 6.594 ± 0.709
1.102GlyHis: 1.102 ± 0.154
4.055GlyIle: 4.055 ± 0.298
3.957GlyLys: 3.957 ± 0.375
5.335GlyLeu: 5.335 ± 0.446
1.614GlyMet: 1.614 ± 0.213
4.291GlyAsn: 4.291 ± 0.474
1.575GlyPro: 1.575 ± 0.236
2.835GlyGln: 2.835 ± 0.27
3.346GlyArg: 3.346 ± 0.27
6.417GlySer: 6.417 ± 0.621
6.516GlyThr: 6.516 ± 0.76
4.862GlyVal: 4.862 ± 0.395
1.043GlyTrp: 1.043 ± 0.156
3.228GlyTyr: 3.228 ± 0.297
0.0GlyXaa: 0.0 ± 0.0
His
0.984HisAla: 0.984 ± 0.162
0.315HisCys: 0.315 ± 0.098
1.28HisAsp: 1.28 ± 0.17
0.984HisGlu: 0.984 ± 0.185
0.689HisPhe: 0.689 ± 0.112
1.201HisGly: 1.201 ± 0.211
0.315HisHis: 0.315 ± 0.087
0.669HisIle: 0.669 ± 0.129
1.28HisLys: 1.28 ± 0.24
1.417HisLeu: 1.417 ± 0.173
0.374HisMet: 0.374 ± 0.088
0.709HisAsn: 0.709 ± 0.128
1.024HisPro: 1.024 ± 0.15
0.65HisGln: 0.65 ± 0.11
0.827HisArg: 0.827 ± 0.172
1.043HisSer: 1.043 ± 0.155
0.925HisThr: 0.925 ± 0.118
0.807HisVal: 0.807 ± 0.146
0.295HisTrp: 0.295 ± 0.087
0.768HisTyr: 0.768 ± 0.132
0.0HisXaa: 0.0 ± 0.0
Ile
4.252IleAla: 4.252 ± 0.32
0.571IleCys: 0.571 ± 0.134
4.311IleAsp: 4.311 ± 0.331
3.681IleGlu: 3.681 ± 0.273
1.654IlePhe: 1.654 ± 0.227
3.524IleGly: 3.524 ± 0.254
0.945IleHis: 0.945 ± 0.168
2.559IleIle: 2.559 ± 0.271
3.209IleLys: 3.209 ± 0.268
3.957IleLeu: 3.957 ± 0.324
1.201IleMet: 1.201 ± 0.196
2.697IleAsn: 2.697 ± 0.233
1.968IlePro: 1.968 ± 0.21
1.831IleGln: 1.831 ± 0.212
2.244IleArg: 2.244 ± 0.218
3.287IleSer: 3.287 ± 0.318
4.665IleThr: 4.665 ± 0.543
3.858IleVal: 3.858 ± 0.355
0.512IleTrp: 0.512 ± 0.115
1.89IleTyr: 1.89 ± 0.155
0.0IleXaa: 0.0 ± 0.0
Lys
3.642LysAla: 3.642 ± 0.351
0.472LysCys: 0.472 ± 0.111
2.697LysAsp: 2.697 ± 0.337
4.567LysGlu: 4.567 ± 0.591
2.539LysPhe: 2.539 ± 0.273
3.583LysGly: 3.583 ± 0.417
1.083LysHis: 1.083 ± 0.184
2.224LysIle: 2.224 ± 0.264
4.744LysLys: 4.744 ± 0.64
4.055LysLeu: 4.055 ± 0.401
1.457LysMet: 1.457 ± 0.239
2.421LysAsn: 2.421 ± 0.268
2.008LysPro: 2.008 ± 0.223
2.441LysGln: 2.441 ± 0.33
2.992LysArg: 2.992 ± 0.385
3.15LysSer: 3.15 ± 0.338
3.051LysThr: 3.051 ± 0.292
4.153LysVal: 4.153 ± 0.413
0.748LysTrp: 0.748 ± 0.161
2.421LysTyr: 2.421 ± 0.29
0.0LysXaa: 0.0 ± 0.0
Leu
5.61LeuAla: 5.61 ± 0.504
0.846LeuCys: 0.846 ± 0.168
5.945LeuAsp: 5.945 ± 0.402
4.646LeuGlu: 4.646 ± 0.436
2.795LeuPhe: 2.795 ± 0.323
5.0LeuGly: 5.0 ± 0.301
1.496LeuHis: 1.496 ± 0.21
3.642LeuIle: 3.642 ± 0.348
4.508LeuLys: 4.508 ± 0.477
5.374LeuLeu: 5.374 ± 0.502
1.772LeuMet: 1.772 ± 0.242
4.429LeuAsn: 4.429 ± 0.313
3.583LeuPro: 3.583 ± 0.313
3.248LeuGln: 3.248 ± 0.321
3.602LeuArg: 3.602 ± 0.3
5.905LeuSer: 5.905 ± 0.42
5.846LeuThr: 5.846 ± 0.485
5.374LeuVal: 5.374 ± 0.364
0.905LeuTrp: 0.905 ± 0.144
3.051LeuTyr: 3.051 ± 0.247
0.0LeuXaa: 0.0 ± 0.0
Met
1.968MetAla: 1.968 ± 0.265
0.197MetCys: 0.197 ± 0.07
1.24MetAsp: 1.24 ± 0.185
1.142MetGlu: 1.142 ± 0.161
0.945MetPhe: 0.945 ± 0.123
1.358MetGly: 1.358 ± 0.165
0.394MetHis: 0.394 ± 0.114
1.181MetIle: 1.181 ± 0.211
1.496MetLys: 1.496 ± 0.213
1.398MetLeu: 1.398 ± 0.191
0.63MetMet: 0.63 ± 0.113
1.083MetAsn: 1.083 ± 0.17
1.004MetPro: 1.004 ± 0.149
0.807MetGln: 0.807 ± 0.162
1.063MetArg: 1.063 ± 0.171
2.067MetSer: 2.067 ± 0.223
1.634MetThr: 1.634 ± 0.239
1.142MetVal: 1.142 ± 0.183
0.315MetTrp: 0.315 ± 0.077
0.571MetTyr: 0.571 ± 0.111
0.0MetXaa: 0.0 ± 0.0
Asn
4.409AsnAla: 4.409 ± 0.51
0.413AsnCys: 0.413 ± 0.1
2.953AsnAsp: 2.953 ± 0.461
2.657AsnGlu: 2.657 ± 0.221
2.146AsnPhe: 2.146 ± 0.225
4.823AsnGly: 4.823 ± 0.52
0.709AsnHis: 0.709 ± 0.161
2.953AsnIle: 2.953 ± 0.231
2.776AsnLys: 2.776 ± 0.382
4.252AsnLeu: 4.252 ± 0.317
0.787AsnMet: 0.787 ± 0.114
2.657AsnAsn: 2.657 ± 0.363
2.835AsnPro: 2.835 ± 0.308
1.673AsnGln: 1.673 ± 0.188
2.224AsnArg: 2.224 ± 0.242
3.169AsnSer: 3.169 ± 0.323
3.228AsnThr: 3.228 ± 0.403
3.858AsnVal: 3.858 ± 0.362
0.768AsnTrp: 0.768 ± 0.18
2.283AsnTyr: 2.283 ± 0.222
0.0AsnXaa: 0.0 ± 0.0
Pro
3.504ProAla: 3.504 ± 0.356
0.256ProCys: 0.256 ± 0.079
2.028ProAsp: 2.028 ± 0.217
3.09ProGlu: 3.09 ± 0.332
2.087ProPhe: 2.087 ± 0.27
3.484ProGly: 3.484 ± 0.272
0.63ProHis: 0.63 ± 0.132
1.752ProIle: 1.752 ± 0.192
1.949ProLys: 1.949 ± 0.234
2.205ProLeu: 2.205 ± 0.273
0.689ProMet: 0.689 ± 0.126
2.047ProAsn: 2.047 ± 0.238
1.201ProPro: 1.201 ± 0.152
1.417ProGln: 1.417 ± 0.209
1.594ProArg: 1.594 ± 0.227
2.756ProSer: 2.756 ± 0.237
3.819ProThr: 3.819 ± 0.273
3.031ProVal: 3.031 ± 0.259
0.453ProTrp: 0.453 ± 0.11
1.122ProTyr: 1.122 ± 0.173
0.0ProXaa: 0.0 ± 0.0
Gln
2.087GlnAla: 2.087 ± 0.2
0.197GlnCys: 0.197 ± 0.087
2.165GlnAsp: 2.165 ± 0.244
2.441GlnGlu: 2.441 ± 0.28
1.437GlnPhe: 1.437 ± 0.185
2.579GlnGly: 2.579 ± 0.234
0.65GlnHis: 0.65 ± 0.112
2.264GlnIle: 2.264 ± 0.227
2.146GlnLys: 2.146 ± 0.313
3.583GlnLeu: 3.583 ± 0.379
1.063GlnMet: 1.063 ± 0.147
1.673GlnAsn: 1.673 ± 0.172
1.437GlnPro: 1.437 ± 0.187
1.693GlnGln: 1.693 ± 0.24
2.067GlnArg: 2.067 ± 0.23
2.421GlnSer: 2.421 ± 0.361
2.756GlnThr: 2.756 ± 0.632
2.776GlnVal: 2.776 ± 0.275
0.669GlnTrp: 0.669 ± 0.118
1.614GlnTyr: 1.614 ± 0.215
0.0GlnXaa: 0.0 ± 0.0
Arg
2.677ArgAla: 2.677 ± 0.308
0.492ArgCys: 0.492 ± 0.105
2.618ArgAsp: 2.618 ± 0.234
2.835ArgGlu: 2.835 ± 0.276
1.772ArgPhe: 1.772 ± 0.224
3.189ArgGly: 3.189 ± 0.307
0.866ArgHis: 0.866 ± 0.153
2.835ArgIle: 2.835 ± 0.302
2.913ArgLys: 2.913 ± 0.382
3.858ArgLeu: 3.858 ± 0.374
1.181ArgMet: 1.181 ± 0.177
2.106ArgAsn: 2.106 ± 0.232
1.85ArgPro: 1.85 ± 0.181
1.614ArgGln: 1.614 ± 0.248
2.323ArgArg: 2.323 ± 0.258
2.795ArgSer: 2.795 ± 0.256
2.5ArgThr: 2.5 ± 0.246
2.579ArgVal: 2.579 ± 0.291
0.472ArgTrp: 0.472 ± 0.111
2.028ArgTyr: 2.028 ± 0.189
0.0ArgXaa: 0.0 ± 0.0
Ser
6.299SerAla: 6.299 ± 0.52
0.453SerCys: 0.453 ± 0.117
4.803SerAsp: 4.803 ± 0.622
3.74SerGlu: 3.74 ± 0.303
2.697SerPhe: 2.697 ± 0.25
6.752SerGly: 6.752 ± 0.587
1.004SerHis: 1.004 ± 0.131
3.917SerIle: 3.917 ± 0.282
3.228SerLys: 3.228 ± 0.397
4.823SerLeu: 4.823 ± 0.308
1.437SerMet: 1.437 ± 0.191
3.504SerAsn: 3.504 ± 0.407
2.402SerPro: 2.402 ± 0.239
2.244SerGln: 2.244 ± 0.276
2.244SerArg: 2.244 ± 0.254
5.669SerSer: 5.669 ± 0.567
6.024SerThr: 6.024 ± 0.626
4.606SerVal: 4.606 ± 0.396
0.571SerTrp: 0.571 ± 0.11
2.598SerTyr: 2.598 ± 0.311
0.0SerXaa: 0.0 ± 0.0
Thr
6.122ThrAla: 6.122 ± 1.049
0.551ThrCys: 0.551 ± 0.119
4.646ThrAsp: 4.646 ± 0.52
4.153ThrGlu: 4.153 ± 0.382
3.051ThrPhe: 3.051 ± 0.261
7.027ThrGly: 7.027 ± 0.887
1.024ThrHis: 1.024 ± 0.203
3.602ThrIle: 3.602 ± 0.315
3.307ThrLys: 3.307 ± 0.269
5.315ThrLeu: 5.315 ± 0.381
0.905ThrMet: 0.905 ± 0.142
4.488ThrAsn: 4.488 ± 0.837
3.543ThrPro: 3.543 ± 0.241
2.598ThrGln: 2.598 ± 0.228
2.264ThrArg: 2.264 ± 0.293
5.689ThrSer: 5.689 ± 0.647
5.531ThrThr: 5.531 ± 0.624
5.61ThrVal: 5.61 ± 0.528
0.65ThrTrp: 0.65 ± 0.115
3.09ThrTyr: 3.09 ± 0.401
0.0ThrXaa: 0.0 ± 0.0
Val
5.256ValAla: 5.256 ± 0.33
0.63ValCys: 0.63 ± 0.158
4.98ValAsp: 4.98 ± 0.418
4.783ValGlu: 4.783 ± 0.469
2.559ValPhe: 2.559 ± 0.257
4.272ValGly: 4.272 ± 0.401
0.984ValHis: 0.984 ± 0.148
3.681ValIle: 3.681 ± 0.322
3.445ValLys: 3.445 ± 0.304
5.413ValLeu: 5.413 ± 0.446
1.634ValMet: 1.634 ± 0.191
3.858ValAsn: 3.858 ± 0.408
2.677ValPro: 2.677 ± 0.265
2.736ValGln: 2.736 ± 0.385
3.169ValArg: 3.169 ± 0.328
5.374ValSer: 5.374 ± 0.413
5.827ValThr: 5.827 ± 0.627
4.862ValVal: 4.862 ± 0.44
0.846ValTrp: 0.846 ± 0.159
2.283ValTyr: 2.283 ± 0.274
0.0ValXaa: 0.0 ± 0.0
Trp
0.925TrpAla: 0.925 ± 0.15
0.236TrpCys: 0.236 ± 0.1
1.22TrpAsp: 1.22 ± 0.168
0.846TrpGlu: 0.846 ± 0.14
0.512TrpPhe: 0.512 ± 0.11
0.65TrpGly: 0.65 ± 0.114
0.217TrpHis: 0.217 ± 0.084
0.728TrpIle: 0.728 ± 0.151
0.335TrpLys: 0.335 ± 0.121
0.768TrpLeu: 0.768 ± 0.143
0.413TrpMet: 0.413 ± 0.095
0.886TrpAsn: 0.886 ± 0.137
0.138TrpPro: 0.138 ± 0.055
0.394TrpGln: 0.394 ± 0.093
0.61TrpArg: 0.61 ± 0.135
1.083TrpSer: 1.083 ± 0.164
0.787TrpThr: 0.787 ± 0.176
1.142TrpVal: 1.142 ± 0.309
0.394TrpTrp: 0.394 ± 0.101
0.807TrpTyr: 0.807 ± 0.19
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.559TyrAla: 2.559 ± 0.271
0.433TyrCys: 0.433 ± 0.114
2.972TyrAsp: 2.972 ± 0.252
2.224TyrGlu: 2.224 ± 0.232
1.693TyrPhe: 1.693 ± 0.197
2.283TyrGly: 2.283 ± 0.278
0.768TyrHis: 0.768 ± 0.142
2.323TyrIle: 2.323 ± 0.26
1.909TyrLys: 1.909 ± 0.222
3.327TyrLeu: 3.327 ± 0.27
0.866TyrMet: 0.866 ± 0.15
2.559TyrAsn: 2.559 ± 0.199
1.24TyrPro: 1.24 ± 0.188
1.811TyrGln: 1.811 ± 0.219
2.047TyrArg: 2.047 ± 0.234
2.402TyrSer: 2.402 ± 0.188
2.933TyrThr: 2.933 ± 0.336
2.913TyrVal: 2.913 ± 0.35
0.65TyrTrp: 0.65 ± 0.117
1.988TyrTyr: 1.988 ± 0.221
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 229 proteins (50802 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski