Clostridium sp. N3C

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M4NDA7|A0A1M4NDA7_9CLOT Membrane dipeptidase (Peptidase family M19) OS=Clostridium sp. N3C OX=1776758 GN=N3C_2222 PE=4 SV=1
MM1 pKa = 7.69AAQNYY6 pKa = 9.28YY7 pKa = 11.04LLTLMSVTPLASDD20 pKa = 4.34PSNKK24 pKa = 9.34IQTVEE29 pKa = 4.17YY30 pKa = 9.97NVKK33 pKa = 8.94LTSDD37 pKa = 3.48NSNVTDD43 pKa = 3.86LAKK46 pKa = 11.08ANFILSSNTSIDD58 pKa = 3.64PADD61 pKa = 3.61WTLDD65 pKa = 3.28TTDD68 pKa = 4.29ASNGNYY74 pKa = 9.99SVTFEE79 pKa = 4.57SGAEE83 pKa = 3.87LDD85 pKa = 3.76VTSYY89 pKa = 11.1LVAYY93 pKa = 9.88VEE95 pKa = 4.32NTGNKK100 pKa = 9.01SDD102 pKa = 3.75KK103 pKa = 10.91LFVDD107 pKa = 5.32FSQALDD113 pKa = 3.64AQGSATYY120 pKa = 9.59SYY122 pKa = 11.43NVSTPGKK129 pKa = 9.3YY130 pKa = 9.94YY131 pKa = 10.63FVLDD135 pKa = 3.78SADD138 pKa = 3.21GVVLNKK144 pKa = 10.16EE145 pKa = 4.15SAEE148 pKa = 4.12SYY150 pKa = 11.03DD151 pKa = 3.98FNNPPDD157 pKa = 4.48PNNSSIAVTFAKK169 pKa = 10.2PYY171 pKa = 9.25LAQIIPDD178 pKa = 4.02PVTGLNVNVIVTLKK192 pKa = 10.69DD193 pKa = 3.54VNDD196 pKa = 3.85TPLIGNNEE204 pKa = 3.73VSFYY208 pKa = 11.56ARR210 pKa = 11.84LEE212 pKa = 4.18SS213 pKa = 3.63

Molecular weight: 23.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4NC77|A0A1M4NC77_9CLOT DNA polymerase III subunit gamma/tau OS=Clostridium sp. N3C OX=1776758 GN=dnaX PE=3 SV=1
MM1 pKa = 7.29ARR3 pKa = 11.84KK4 pKa = 9.71AIIEE8 pKa = 4.08KK9 pKa = 9.11WKK11 pKa = 10.78KK12 pKa = 7.55EE13 pKa = 3.85PKK15 pKa = 8.7YY16 pKa = 8.29QTRR19 pKa = 11.84AYY21 pKa = 7.9TRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 6.44AVLRR35 pKa = 11.84KK36 pKa = 9.63YY37 pKa = 9.83GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.88KK50 pKa = 10.14GQIPGCRR57 pKa = 11.84KK58 pKa = 10.14ASWW61 pKa = 3.01

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2848

0

2848

868247

29

2934

304.9

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.039 ± 0.047

1.135 ± 0.018

5.537 ± 0.03

7.622 ± 0.051

4.238 ± 0.035

6.267 ± 0.038

1.44 ± 0.017

9.529 ± 0.056

8.769 ± 0.037

9.238 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.579 ± 0.02

5.815 ± 0.041

2.867 ± 0.026

2.563 ± 0.022

3.665 ± 0.034

6.332 ± 0.041

4.769 ± 0.036

6.595 ± 0.041

0.701 ± 0.015

4.3 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski