Tentweb spider associated circular virus 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BPD1|A0A346BPD1_9VIRU Putative capsid protein OS=Tentweb spider associated circular virus 1 OX=2293307 PE=4 SV=1
MM1 pKa = 7.56PRR3 pKa = 11.84QARR6 pKa = 11.84VKK8 pKa = 9.72CWCFTLNNYY17 pKa = 8.59TEE19 pKa = 5.57DD20 pKa = 3.6EE21 pKa = 4.41FLTLQRR27 pKa = 11.84TLEE30 pKa = 4.1LQKK33 pKa = 9.86PTFAIIAKK41 pKa = 9.72EE42 pKa = 3.99VGDD45 pKa = 3.92NQTPHH50 pKa = 5.83LQGYY54 pKa = 7.17VHH56 pKa = 6.33LTKK59 pKa = 10.07RR60 pKa = 11.84TVFSDD65 pKa = 3.19VKK67 pKa = 10.53KK68 pKa = 10.16ILGIRR73 pKa = 11.84AHH75 pKa = 6.68IEE77 pKa = 3.55QARR80 pKa = 11.84GSDD83 pKa = 3.58QDD85 pKa = 3.77SLLYY89 pKa = 10.36CSKK92 pKa = 10.61QDD94 pKa = 3.73PNPFIIGAPDD104 pKa = 3.78LKK106 pKa = 10.98SGGSAKK112 pKa = 10.32RR113 pKa = 11.84IALHH117 pKa = 6.31RR118 pKa = 11.84AASEE122 pKa = 4.15WIAGVTGGEE131 pKa = 4.51SIFHH135 pKa = 6.34QFTKK139 pKa = 10.82SPDD142 pKa = 2.97SGMAYY147 pKa = 9.93LRR149 pKa = 11.84HH150 pKa = 6.14FKK152 pKa = 10.55AIEE155 pKa = 4.25NIVKK159 pKa = 10.14DD160 pKa = 3.38KK161 pKa = 11.19RR162 pKa = 11.84RR163 pKa = 11.84IHH165 pKa = 7.41SMDD168 pKa = 4.14KK169 pKa = 10.38IRR171 pKa = 11.84EE172 pKa = 4.12EE173 pKa = 4.15YY174 pKa = 10.29TNATLRR180 pKa = 11.84IYY182 pKa = 8.81QARR185 pKa = 11.84IDD187 pKa = 4.1RR188 pKa = 11.84MVKK191 pKa = 10.44LPPDD195 pKa = 3.76PRR197 pKa = 11.84TIHH200 pKa = 5.76WFYY203 pKa = 11.4DD204 pKa = 3.31EE205 pKa = 4.99RR206 pKa = 11.84GNTGKK211 pKa = 10.06SWMTNWLMLNHH222 pKa = 6.82GAICFSNGKK231 pKa = 9.35SADD234 pKa = 2.92IAYY237 pKa = 9.37AYY239 pKa = 10.18SGEE242 pKa = 4.23RR243 pKa = 11.84IVVFDD248 pKa = 4.66FTRR251 pKa = 11.84SMQDD255 pKa = 2.91VLNYY259 pKa = 9.88QVIEE263 pKa = 3.95EE264 pKa = 4.57LKK266 pKa = 10.02NGRR269 pKa = 11.84IFSPKK274 pKa = 10.02YY275 pKa = 9.73EE276 pKa = 4.37SCCKK280 pKa = 10.21LFPRR284 pKa = 11.84PHH286 pKa = 6.76VICFANWLPDD296 pKa = 3.79YY297 pKa = 11.17GKK299 pKa = 9.95MSADD303 pKa = 2.79RR304 pKa = 11.84WDD306 pKa = 3.01IHH308 pKa = 6.91EE309 pKa = 5.55ILDD312 pKa = 3.87EE313 pKa = 4.65DD314 pKa = 4.98CEE316 pKa = 4.43QEE318 pKa = 3.83TDD320 pKa = 3.18QDD322 pKa = 3.91QVEE325 pKa = 4.36LEE327 pKa = 3.91NFIFEE332 pKa = 4.46KK333 pKa = 10.92

Molecular weight: 38.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BPD1|A0A346BPD1_9VIRU Putative capsid protein OS=Tentweb spider associated circular virus 1 OX=2293307 PE=4 SV=1
MM1 pKa = 8.03DD2 pKa = 4.48EE3 pKa = 3.87NVRR6 pKa = 11.84RR7 pKa = 11.84ILHH10 pKa = 6.88DD11 pKa = 4.61IIQQRR16 pKa = 11.84EE17 pKa = 3.68DD18 pKa = 3.57GYY20 pKa = 11.05RR21 pKa = 11.84SGMSAAIRR29 pKa = 11.84GIDD32 pKa = 3.66QSWSIPATGPFTTKK46 pKa = 9.79IRR48 pKa = 11.84FSLGDD53 pKa = 3.7LMKK56 pKa = 10.89SSATQYY62 pKa = 10.89SEE64 pKa = 5.84EE65 pKa = 4.14YY66 pKa = 8.24WQLEE70 pKa = 4.3GYY72 pKa = 7.88YY73 pKa = 10.23TIFDD77 pKa = 3.44QVKK80 pKa = 8.0VVKK83 pKa = 10.93VMFEE87 pKa = 3.79YY88 pKa = 10.4WLTDD92 pKa = 3.42DD93 pKa = 5.88DD94 pKa = 6.21VEE96 pKa = 4.58TTPNAAFVRR105 pKa = 11.84QVYY108 pKa = 10.47SYY110 pKa = 11.45DD111 pKa = 3.69PDD113 pKa = 3.9SADD116 pKa = 5.88GNMTWDD122 pKa = 3.41NVIRR126 pKa = 11.84VPDD129 pKa = 3.73HH130 pKa = 5.86AHH132 pKa = 7.19RR133 pKa = 11.84ILKK136 pKa = 10.02PGVIYY141 pKa = 8.66RR142 pKa = 11.84TSLKK146 pKa = 10.57PKK148 pKa = 10.04FAVQLATPNGTFKK161 pKa = 10.94GYY163 pKa = 10.44SKK165 pKa = 11.33NMWIDD170 pKa = 3.67CGDD173 pKa = 3.33VAGYY177 pKa = 9.73DD178 pKa = 3.43QAKK181 pKa = 10.1SVLGSVNAIHH191 pKa = 6.4MAFKK195 pKa = 10.78GPEE198 pKa = 3.7EE199 pKa = 4.34LGAIGYY205 pKa = 9.03RR206 pKa = 11.84RR207 pKa = 11.84TLFLKK212 pKa = 10.09FRR214 pKa = 11.84KK215 pKa = 9.15RR216 pKa = 11.84RR217 pKa = 11.84QGAVVNN223 pKa = 4.41

Molecular weight: 25.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

556

223

333

278.0

32.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.655 ± 0.29

1.619 ± 0.653

7.194 ± 0.239

5.576 ± 0.859

4.856 ± 0.207

6.295 ± 0.741

2.698 ± 0.504

7.194 ± 0.511

6.295 ± 0.26

6.295 ± 0.51

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.698 ± 0.246

3.957 ± 0.206

4.137 ± 0.056

4.496 ± 0.007

6.655 ± 0.04

5.935 ± 0.191

5.396 ± 0.242

5.576 ± 1.142

2.158 ± 0.047

4.317 ± 0.594
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski