Amino acid dipepetide frequency for Enterococcus phage Idefix

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.049AlaAla: 1.049 ± 0.527
0.524AlaCys: 0.524 ± 0.271
3.146AlaAsp: 3.146 ± 0.673
2.797AlaGlu: 2.797 ± 0.853
2.447AlaPhe: 2.447 ± 0.517
2.972AlaGly: 2.972 ± 0.787
0.699AlaHis: 0.699 ± 0.384
4.02AlaIle: 4.02 ± 0.936
2.797AlaLys: 2.797 ± 0.761
4.894AlaLeu: 4.894 ± 0.685
2.098AlaMet: 2.098 ± 0.561
3.496AlaAsn: 3.496 ± 0.696
2.098AlaPro: 2.098 ± 0.654
2.447AlaGln: 2.447 ± 0.712
1.923AlaArg: 1.923 ± 0.393
2.972AlaSer: 2.972 ± 0.573
3.146AlaThr: 3.146 ± 0.659
2.447AlaVal: 2.447 ± 0.54
0.699AlaTrp: 0.699 ± 0.394
2.972AlaTyr: 2.972 ± 0.689
0.0AlaXaa: 0.0 ± 0.0
Cys
0.35CysAla: 0.35 ± 0.226
0.0CysCys: 0.0 ± 0.0
0.699CysAsp: 0.699 ± 0.319
0.874CysGlu: 0.874 ± 0.288
0.524CysPhe: 0.524 ± 0.281
0.874CysGly: 0.874 ± 0.534
0.175CysHis: 0.175 ± 0.188
0.524CysIle: 0.524 ± 0.288
0.35CysLys: 0.35 ± 0.289
0.524CysLeu: 0.524 ± 0.317
0.175CysMet: 0.175 ± 0.176
0.874CysAsn: 0.874 ± 0.316
0.175CysPro: 0.175 ± 0.148
0.524CysGln: 0.524 ± 0.317
0.35CysArg: 0.35 ± 0.248
0.35CysSer: 0.35 ± 0.218
0.524CysThr: 0.524 ± 0.353
0.874CysVal: 0.874 ± 0.412
0.0CysTrp: 0.0 ± 0.0
0.35CysTyr: 0.35 ± 0.262
0.0CysXaa: 0.0 ± 0.0
Asp
1.573AspAla: 1.573 ± 0.449
0.874AspCys: 0.874 ± 0.388
1.923AspAsp: 1.923 ± 0.763
4.894AspGlu: 4.894 ± 1.177
3.845AspPhe: 3.845 ± 0.584
4.37AspGly: 4.37 ± 0.933
0.35AspHis: 0.35 ± 0.243
4.894AspIle: 4.894 ± 0.892
4.195AspLys: 4.195 ± 0.786
6.992AspLeu: 6.992 ± 0.955
1.224AspMet: 1.224 ± 0.446
4.195AspAsn: 4.195 ± 0.69
2.447AspPro: 2.447 ± 0.827
1.049AspGln: 1.049 ± 0.487
2.098AspArg: 2.098 ± 0.648
3.146AspSer: 3.146 ± 0.827
3.845AspThr: 3.845 ± 0.837
4.545AspVal: 4.545 ± 0.936
0.524AspTrp: 0.524 ± 0.295
4.02AspTyr: 4.02 ± 0.62
0.0AspXaa: 0.0 ± 0.0
Glu
3.321GluAla: 3.321 ± 0.809
0.874GluCys: 0.874 ± 0.578
4.195GluAsp: 4.195 ± 1.038
7.167GluGlu: 7.167 ± 1.572
3.845GluPhe: 3.845 ± 0.855
3.321GluGly: 3.321 ± 0.714
1.398GluHis: 1.398 ± 0.393
6.118GluIle: 6.118 ± 1.459
5.943GluLys: 5.943 ± 1.385
4.894GluLeu: 4.894 ± 1.219
2.622GluMet: 2.622 ± 0.571
4.894GluAsn: 4.894 ± 0.9
1.923GluPro: 1.923 ± 0.859
3.496GluGln: 3.496 ± 0.515
3.496GluArg: 3.496 ± 0.726
3.146GluSer: 3.146 ± 1.031
5.069GluThr: 5.069 ± 0.998
4.719GluVal: 4.719 ± 0.901
1.224GluTrp: 1.224 ± 0.55
4.02GluTyr: 4.02 ± 1.049
0.0GluXaa: 0.0 ± 0.0
Phe
2.098PheAla: 2.098 ± 0.397
0.524PheCys: 0.524 ± 0.312
3.671PheAsp: 3.671 ± 1.081
2.622PheGlu: 2.622 ± 0.612
1.573PhePhe: 1.573 ± 0.599
3.496PheGly: 3.496 ± 1.167
0.524PheHis: 0.524 ± 0.346
5.419PheIle: 5.419 ± 1.071
2.272PheLys: 2.272 ± 0.745
4.195PheLeu: 4.195 ± 1.024
1.224PheMet: 1.224 ± 0.385
4.545PheAsn: 4.545 ± 1.205
1.748PhePro: 1.748 ± 0.432
1.049PheGln: 1.049 ± 0.436
2.098PheArg: 2.098 ± 0.507
2.972PheSer: 2.972 ± 1.097
3.671PheThr: 3.671 ± 0.997
2.797PheVal: 2.797 ± 0.796
0.35PheTrp: 0.35 ± 0.247
2.272PheTyr: 2.272 ± 0.74
0.0PheXaa: 0.0 ± 0.0
Gly
5.069GlyAla: 5.069 ± 1.777
0.524GlyCys: 0.524 ± 0.251
4.195GlyAsp: 4.195 ± 1.269
4.195GlyGlu: 4.195 ± 1.105
2.622GlyPhe: 2.622 ± 0.772
6.992GlyGly: 6.992 ± 1.099
1.573GlyHis: 1.573 ± 0.593
4.195GlyIle: 4.195 ± 0.941
4.37GlyLys: 4.37 ± 0.701
4.37GlyLeu: 4.37 ± 1.1
1.398GlyMet: 1.398 ± 0.61
4.37GlyAsn: 4.37 ± 1.056
0.35GlyPro: 0.35 ± 0.235
1.049GlyGln: 1.049 ± 0.541
1.224GlyArg: 1.224 ± 0.325
3.845GlySer: 3.845 ± 1.266
3.845GlyThr: 3.845 ± 0.559
4.545GlyVal: 4.545 ± 0.839
0.524GlyTrp: 0.524 ± 0.371
2.622GlyTyr: 2.622 ± 0.864
0.0GlyXaa: 0.0 ± 0.0
His
0.874HisAla: 0.874 ± 0.388
0.175HisCys: 0.175 ± 0.165
0.874HisAsp: 0.874 ± 0.358
1.224HisGlu: 1.224 ± 0.532
1.573HisPhe: 1.573 ± 0.526
0.874HisGly: 0.874 ± 0.555
0.175HisHis: 0.175 ± 0.148
1.224HisIle: 1.224 ± 0.315
0.35HisLys: 0.35 ± 0.228
1.224HisLeu: 1.224 ± 0.361
0.35HisMet: 0.35 ± 0.231
1.748HisAsn: 1.748 ± 0.441
0.524HisPro: 0.524 ± 0.378
0.175HisGln: 0.175 ± 0.165
1.049HisArg: 1.049 ± 0.572
0.874HisSer: 0.874 ± 0.352
0.175HisThr: 0.175 ± 0.186
0.874HisVal: 0.874 ± 0.372
0.175HisTrp: 0.175 ± 0.2
1.573HisTyr: 1.573 ± 0.387
0.0HisXaa: 0.0 ± 0.0
Ile
4.894IleAla: 4.894 ± 0.97
0.874IleCys: 0.874 ± 0.342
4.894IleAsp: 4.894 ± 0.821
5.593IleGlu: 5.593 ± 1.2
2.622IlePhe: 2.622 ± 0.934
3.671IleGly: 3.671 ± 0.66
1.573IleHis: 1.573 ± 0.477
3.496IleIle: 3.496 ± 0.794
5.768IleLys: 5.768 ± 1.046
4.195IleLeu: 4.195 ± 1.005
0.699IleMet: 0.699 ± 0.417
5.419IleAsn: 5.419 ± 0.812
2.972IlePro: 2.972 ± 0.657
3.146IleGln: 3.146 ± 0.837
1.224IleArg: 1.224 ± 0.431
4.894IleSer: 4.894 ± 0.852
4.545IleThr: 4.545 ± 0.962
3.845IleVal: 3.845 ± 0.88
0.874IleTrp: 0.874 ± 0.437
2.447IleTyr: 2.447 ± 0.618
0.0IleXaa: 0.0 ± 0.0
Lys
4.719LysAla: 4.719 ± 1.168
0.699LysCys: 0.699 ± 0.49
4.37LysAsp: 4.37 ± 0.721
6.817LysGlu: 6.817 ± 1.539
4.37LysPhe: 4.37 ± 1.046
4.37LysGly: 4.37 ± 0.712
1.049LysHis: 1.049 ± 0.344
5.069LysIle: 5.069 ± 0.775
6.293LysLys: 6.293 ± 1.099
7.516LysLeu: 7.516 ± 1.177
2.622LysMet: 2.622 ± 0.696
3.321LysAsn: 3.321 ± 0.836
2.972LysPro: 2.972 ± 0.798
2.622LysGln: 2.622 ± 0.547
3.496LysArg: 3.496 ± 0.685
3.321LysSer: 3.321 ± 0.798
5.069LysThr: 5.069 ± 1.038
6.817LysVal: 6.817 ± 0.81
0.874LysTrp: 0.874 ± 0.391
3.671LysTyr: 3.671 ± 0.809
0.0LysXaa: 0.0 ± 0.0
Leu
5.069LeuAla: 5.069 ± 0.627
0.699LeuCys: 0.699 ± 0.407
4.894LeuAsp: 4.894 ± 0.858
6.817LeuGlu: 6.817 ± 1.193
0.699LeuPhe: 0.699 ± 0.331
4.894LeuGly: 4.894 ± 1.17
1.049LeuHis: 1.049 ± 0.422
4.719LeuIle: 4.719 ± 0.707
8.39LeuLys: 8.39 ± 1.362
6.293LeuLeu: 6.293 ± 1.502
2.272LeuMet: 2.272 ± 0.634
6.118LeuAsn: 6.118 ± 1.106
4.02LeuPro: 4.02 ± 0.827
1.573LeuGln: 1.573 ± 0.599
2.797LeuArg: 2.797 ± 0.655
5.069LeuSer: 5.069 ± 0.781
4.719LeuThr: 4.719 ± 0.893
4.719LeuVal: 4.719 ± 0.785
0.699LeuTrp: 0.699 ± 0.363
3.671LeuTyr: 3.671 ± 0.813
0.0LeuXaa: 0.0 ± 0.0
Met
1.049MetAla: 1.049 ± 0.358
0.0MetCys: 0.0 ± 0.0
1.224MetAsp: 1.224 ± 0.597
1.224MetGlu: 1.224 ± 0.384
2.098MetPhe: 2.098 ± 0.39
1.923MetGly: 1.923 ± 0.399
0.0MetHis: 0.0 ± 0.0
2.447MetIle: 2.447 ± 0.679
3.321MetLys: 3.321 ± 0.633
2.972MetLeu: 2.972 ± 0.772
0.524MetMet: 0.524 ± 0.264
2.098MetAsn: 2.098 ± 0.435
0.699MetPro: 0.699 ± 0.325
0.874MetGln: 0.874 ± 0.389
1.049MetArg: 1.049 ± 0.565
1.923MetSer: 1.923 ± 0.749
2.272MetThr: 2.272 ± 0.78
1.224MetVal: 1.224 ± 0.349
0.175MetTrp: 0.175 ± 0.227
1.398MetTyr: 1.398 ± 0.503
0.0MetXaa: 0.0 ± 0.0
Asn
4.195AsnAla: 4.195 ± 0.923
0.0AsnCys: 0.0 ± 0.0
2.622AsnAsp: 2.622 ± 0.822
5.244AsnGlu: 5.244 ± 1.104
3.496AsnPhe: 3.496 ± 0.926
4.719AsnGly: 4.719 ± 1.182
1.923AsnHis: 1.923 ± 0.54
4.37AsnIle: 4.37 ± 0.901
5.768AsnLys: 5.768 ± 1.057
5.069AsnLeu: 5.069 ± 0.673
2.797AsnMet: 2.797 ± 0.722
7.167AsnAsn: 7.167 ± 1.338
4.02AsnPro: 4.02 ± 1.028
2.972AsnGln: 2.972 ± 0.655
1.748AsnArg: 1.748 ± 0.545
4.545AsnSer: 4.545 ± 0.745
3.146AsnThr: 3.146 ± 0.809
3.146AsnVal: 3.146 ± 0.942
1.224AsnTrp: 1.224 ± 0.456
3.845AsnTyr: 3.845 ± 0.691
0.0AsnXaa: 0.0 ± 0.0
Pro
1.398ProAla: 1.398 ± 0.432
0.0ProCys: 0.0 ± 0.0
3.321ProAsp: 3.321 ± 0.781
2.972ProGlu: 2.972 ± 0.503
2.272ProPhe: 2.272 ± 0.493
0.524ProGly: 0.524 ± 0.265
0.35ProHis: 0.35 ± 0.187
1.748ProIle: 1.748 ± 0.601
3.845ProLys: 3.845 ± 0.903
1.923ProLeu: 1.923 ± 0.457
0.175ProMet: 0.175 ± 0.15
2.797ProAsn: 2.797 ± 1.018
1.049ProPro: 1.049 ± 0.764
1.224ProGln: 1.224 ± 0.451
0.874ProArg: 0.874 ± 0.572
3.321ProSer: 3.321 ± 0.815
3.146ProThr: 3.146 ± 0.677
1.398ProVal: 1.398 ± 0.523
0.35ProTrp: 0.35 ± 0.205
1.224ProTyr: 1.224 ± 0.476
0.0ProXaa: 0.0 ± 0.0
Gln
1.573GlnAla: 1.573 ± 0.477
0.175GlnCys: 0.175 ± 0.176
1.049GlnAsp: 1.049 ± 0.431
1.748GlnGlu: 1.748 ± 0.425
2.447GlnPhe: 2.447 ± 0.485
2.622GlnGly: 2.622 ± 0.613
0.524GlnHis: 0.524 ± 0.269
2.098GlnIle: 2.098 ± 0.448
3.321GlnLys: 3.321 ± 0.49
2.972GlnLeu: 2.972 ± 0.612
0.699GlnMet: 0.699 ± 0.269
2.622GlnAsn: 2.622 ± 0.516
0.874GlnPro: 0.874 ± 0.39
1.398GlnGln: 1.398 ± 0.498
1.398GlnArg: 1.398 ± 0.537
1.224GlnSer: 1.224 ± 0.52
3.321GlnThr: 3.321 ± 1.009
1.923GlnVal: 1.923 ± 0.534
1.573GlnTrp: 1.573 ± 0.522
1.224GlnTyr: 1.224 ± 0.476
0.0GlnXaa: 0.0 ± 0.0
Arg
0.874ArgAla: 0.874 ± 0.392
0.699ArgCys: 0.699 ± 0.302
1.923ArgAsp: 1.923 ± 0.385
1.923ArgGlu: 1.923 ± 0.536
2.447ArgPhe: 2.447 ± 0.808
1.923ArgGly: 1.923 ± 0.474
0.35ArgHis: 0.35 ± 0.224
2.447ArgIle: 2.447 ± 0.53
2.447ArgLys: 2.447 ± 0.677
2.972ArgLeu: 2.972 ± 0.661
0.874ArgMet: 0.874 ± 0.521
2.272ArgAsn: 2.272 ± 0.473
0.699ArgPro: 0.699 ± 0.276
2.272ArgGln: 2.272 ± 0.617
0.874ArgArg: 0.874 ± 0.422
1.573ArgSer: 1.573 ± 0.552
1.049ArgThr: 1.049 ± 0.423
2.098ArgVal: 2.098 ± 0.678
0.175ArgTrp: 0.175 ± 0.175
2.972ArgTyr: 2.972 ± 0.609
0.0ArgXaa: 0.0 ± 0.0
Ser
2.447SerAla: 2.447 ± 0.716
0.0SerCys: 0.0 ± 0.0
5.069SerAsp: 5.069 ± 1.091
4.195SerGlu: 4.195 ± 0.802
2.972SerPhe: 2.972 ± 0.69
3.671SerGly: 3.671 ± 1.154
1.398SerHis: 1.398 ± 0.412
4.719SerIle: 4.719 ± 0.822
5.069SerLys: 5.069 ± 0.722
4.37SerLeu: 4.37 ± 1.073
2.272SerMet: 2.272 ± 0.736
3.146SerAsn: 3.146 ± 1.068
1.049SerPro: 1.049 ± 0.437
2.098SerGln: 2.098 ± 0.46
1.398SerArg: 1.398 ± 0.543
3.496SerSer: 3.496 ± 0.605
3.496SerThr: 3.496 ± 0.946
2.447SerVal: 2.447 ± 0.743
1.049SerTrp: 1.049 ± 0.564
2.972SerTyr: 2.972 ± 0.993
0.0SerXaa: 0.0 ± 0.0
Thr
3.321ThrAla: 3.321 ± 0.998
0.175ThrCys: 0.175 ± 0.15
3.671ThrAsp: 3.671 ± 1.135
6.293ThrGlu: 6.293 ± 0.83
3.845ThrPhe: 3.845 ± 0.89
4.545ThrGly: 4.545 ± 0.865
1.398ThrHis: 1.398 ± 0.429
4.195ThrIle: 4.195 ± 0.534
5.419ThrLys: 5.419 ± 1.024
4.894ThrLeu: 4.894 ± 0.878
2.098ThrMet: 2.098 ± 0.659
4.195ThrAsn: 4.195 ± 0.894
2.098ThrPro: 2.098 ± 0.827
2.797ThrGln: 2.797 ± 0.914
1.573ThrArg: 1.573 ± 0.415
4.02ThrSer: 4.02 ± 0.678
5.419ThrThr: 5.419 ± 1.345
4.719ThrVal: 4.719 ± 0.836
0.524ThrTrp: 0.524 ± 0.321
2.972ThrTyr: 2.972 ± 0.505
0.0ThrXaa: 0.0 ± 0.0
Val
3.146ValAla: 3.146 ± 0.781
0.699ValCys: 0.699 ± 0.249
5.244ValAsp: 5.244 ± 1.342
5.593ValGlu: 5.593 ± 1.048
1.923ValPhe: 1.923 ± 0.446
2.972ValGly: 2.972 ± 0.857
0.35ValHis: 0.35 ± 0.327
2.972ValIle: 2.972 ± 0.479
5.419ValLys: 5.419 ± 0.737
3.146ValLeu: 3.146 ± 0.705
2.447ValMet: 2.447 ± 0.5
3.496ValAsn: 3.496 ± 0.723
2.622ValPro: 2.622 ± 0.627
0.524ValGln: 0.524 ± 0.3
2.098ValArg: 2.098 ± 0.531
3.146ValSer: 3.146 ± 0.838
5.419ValThr: 5.419 ± 1.097
3.146ValVal: 3.146 ± 0.782
0.524ValTrp: 0.524 ± 0.267
4.02ValTyr: 4.02 ± 0.632
0.0ValXaa: 0.0 ± 0.0
Trp
0.524TrpAla: 0.524 ± 0.317
0.524TrpCys: 0.524 ± 0.382
1.224TrpAsp: 1.224 ± 0.486
0.175TrpGlu: 0.175 ± 0.175
0.874TrpPhe: 0.874 ± 0.428
0.175TrpGly: 0.175 ± 0.165
0.35TrpHis: 0.35 ± 0.209
1.224TrpIle: 1.224 ± 0.441
0.35TrpLys: 0.35 ± 0.248
1.398TrpLeu: 1.398 ± 0.521
0.524TrpMet: 0.524 ± 0.296
0.524TrpAsn: 0.524 ± 0.407
0.0TrpPro: 0.0 ± 0.0
0.699TrpGln: 0.699 ± 0.319
0.874TrpArg: 0.874 ± 0.416
0.35TrpSer: 0.35 ± 0.27
1.398TrpThr: 1.398 ± 0.394
0.35TrpVal: 0.35 ± 0.351
0.0TrpTrp: 0.0 ± 0.0
0.874TrpTyr: 0.874 ± 0.332
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.098TyrAla: 2.098 ± 0.465
1.049TyrCys: 1.049 ± 0.45
2.797TyrAsp: 2.797 ± 0.906
3.321TyrGlu: 3.321 ± 0.876
2.622TyrPhe: 2.622 ± 0.617
2.972TyrGly: 2.972 ± 0.612
0.874TyrHis: 0.874 ± 0.357
1.923TyrIle: 1.923 ± 0.599
4.37TyrLys: 4.37 ± 0.988
4.37TyrLeu: 4.37 ± 0.839
1.224TyrMet: 1.224 ± 0.346
4.719TyrAsn: 4.719 ± 0.801
1.573TyrPro: 1.573 ± 0.437
2.797TyrGln: 2.797 ± 0.477
1.224TyrArg: 1.224 ± 0.389
3.146TyrSer: 3.146 ± 0.453
4.894TyrThr: 4.894 ± 0.685
2.272TyrVal: 2.272 ± 0.586
0.874TyrTrp: 0.874 ± 0.324
2.622TyrTyr: 2.622 ± 0.81
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 25 proteins (5722 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski