Amino acid dipepetide frequency for Actinobacteria bacterium OV320

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.013AlaAla: 21.013 ± 0.141	1.064AlaCys: 1.064 ± 0.019	8.422AlaAsp: 8.422 ± 0.065	8.598AlaGlu: 8.598 ± 0.088	3.472AlaPhe: 3.472 ± 0.039	12.946AlaGly: 12.946 ± 0.08	2.935AlaHis: 2.935 ± 0.036	3.399AlaIle: 3.399 ± 0.038	2.877AlaLys: 2.877 ± 0.038	14.367AlaLeu: 14.367 ± 0.115	2.38AlaMet: 2.38 ± 0.033	1.967AlaAsn: 1.967 ± 0.031	7.13AlaPro: 7.13 ± 0.065	3.856AlaGln: 3.856 ± 0.036	10.285AlaArg: 10.285 ± 0.079	6.162AlaSer: 6.162 ± 0.056	7.311AlaThr: 7.311 ± 0.059	12.312AlaVal: 12.312 ± 0.081	1.88AlaTrp: 1.88 ± 0.028	2.84AlaTyr: 2.84 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	1.084CysAla: 1.084 ± 0.019	0.096CysCys: 0.096 ± 0.007	0.453CysAsp: 0.453 ± 0.011	0.394CysGlu: 0.394 ± 0.013	0.215CysPhe: 0.215 ± 0.009	0.95CysGly: 0.95 ± 0.019	0.207CysHis: 0.207 ± 0.008	0.168CysIle: 0.168 ± 0.007	0.11CysLys: 0.11 ± 0.006	0.745CysLeu: 0.745 ± 0.017	0.118CysMet: 0.118 ± 0.006	0.127CysAsn: 0.127 ± 0.006	0.47CysPro: 0.47 ± 0.012	0.158CysGln: 0.158 ± 0.006	0.588CysArg: 0.588 ± 0.014	0.424CysSer: 0.424 ± 0.011	0.515CysThr: 0.515 ± 0.015	0.694CysVal: 0.694 ± 0.018	0.137CysTrp: 0.137 ± 0.007	0.154CysTyr: 0.154 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.595AspAla: 7.595 ± 0.053	0.422AspCys: 0.422 ± 0.013	3.731AspAsp: 3.731 ± 0.042	3.844AspGlu: 3.844 ± 0.038	1.644AspPhe: 1.644 ± 0.024	6.54AspGly: 6.54 ± 0.054	1.437AspHis: 1.437 ± 0.025	1.866AspIle: 1.866 ± 0.027	1.193AspLys: 1.193 ± 0.025	6.197AspLeu: 6.197 ± 0.053	0.811AspMet: 0.811 ± 0.016	0.998AspAsn: 0.998 ± 0.02	4.521AspPro: 4.521 ± 0.042	1.533AspGln: 1.533 ± 0.027	4.929AspArg: 4.929 ± 0.044	2.657AspSer: 2.657 ± 0.029	3.388AspThr: 3.388 ± 0.037	4.681AspVal: 4.681 ± 0.041	1.066AspTrp: 1.066 ± 0.023	1.132AspTyr: 1.132 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	7.286GluAla: 7.286 ± 0.07	0.347GluCys: 0.347 ± 0.012	2.768GluAsp: 2.768 ± 0.032	3.542GluGlu: 3.542 ± 0.046	1.482GluPhe: 1.482 ± 0.024	4.305GluGly: 4.305 ± 0.043	1.547GluHis: 1.547 ± 0.027	2.22GluIle: 2.22 ± 0.033	1.399GluLys: 1.399 ± 0.025	6.684GluLeu: 6.684 ± 0.057	0.845GluMet: 0.845 ± 0.02	1.064GluAsn: 1.064 ± 0.018	3.392GluPro: 3.392 ± 0.041	2.219GluGln: 2.219 ± 0.033	5.283GluArg: 5.283 ± 0.048	2.641GluSer: 2.641 ± 0.037	2.93GluThr: 2.93 ± 0.032	4.365GluVal: 4.365 ± 0.042	0.786GluTrp: 0.786 ± 0.015	1.119GluTyr: 1.119 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.629PheAla: 3.629 ± 0.037	0.245PheCys: 0.245 ± 0.01	1.916PheAsp: 1.916 ± 0.025	1.428PheGlu: 1.428 ± 0.027	0.866PhePhe: 0.866 ± 0.021	2.963PheGly: 2.963 ± 0.036	0.608PheHis: 0.608 ± 0.015	0.671PheIle: 0.671 ± 0.016	0.536PheLys: 0.536 ± 0.014	2.594PheLeu: 2.594 ± 0.034	0.396PheMet: 0.396 ± 0.011	0.588PheAsn: 0.588 ± 0.015	1.374PhePro: 1.374 ± 0.021	0.691PheGln: 0.691 ± 0.017	1.844PheArg: 1.844 ± 0.028	1.445PheSer: 1.445 ± 0.023	2.023PheThr: 2.023 ± 0.027	2.274PheVal: 2.274 ± 0.025	0.42PheTrp: 0.42 ± 0.01	0.597PheTyr: 0.597 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	11.129GlyAla: 11.129 ± 0.072	0.829GlyCys: 0.829 ± 0.02	5.185GlyAsp: 5.185 ± 0.04	5.085GlyGlu: 5.085 ± 0.047	2.868GlyPhe: 2.868 ± 0.034	8.834GlyGly: 8.834 ± 0.072	2.321GlyHis: 2.321 ± 0.027	3.407GlyIle: 3.407 ± 0.035	2.399GlyLys: 2.399 ± 0.034	9.325GlyLeu: 9.325 ± 0.067	1.889GlyMet: 1.889 ± 0.031	1.817GlyAsn: 1.817 ± 0.033	5.246GlyPro: 5.246 ± 0.048	2.701GlyGln: 2.701 ± 0.037	7.704GlyArg: 7.704 ± 0.054	5.572GlySer: 5.572 ± 0.048	6.632GlyThr: 6.632 ± 0.062	7.563GlyVal: 7.563 ± 0.052	1.738GlyTrp: 1.738 ± 0.026	2.3GlyTyr: 2.3 ± 0.03	0.0GlyXaa: 0.0 ± 0.0
His	2.75HisAla: 2.75 ± 0.038	0.212HisCys: 0.212 ± 0.009	1.373HisAsp: 1.373 ± 0.024	1.252HisGlu: 1.252 ± 0.021	0.631HisPhe: 0.631 ± 0.016	2.408HisGly: 2.408 ± 0.031	0.713HisHis: 0.713 ± 0.019	0.69HisIle: 0.69 ± 0.014	0.35HisLys: 0.35 ± 0.012	2.359HisLeu: 2.359 ± 0.03	0.329HisMet: 0.329 ± 0.011	0.397HisAsn: 0.397 ± 0.012	1.839HisPro: 1.839 ± 0.026	0.65HisGln: 0.65 ± 0.016	2.093HisArg: 2.093 ± 0.031	1.023HisSer: 1.023 ± 0.02	1.405HisThr: 1.405 ± 0.023	1.71HisVal: 1.71 ± 0.024	0.386HisTrp: 0.386 ± 0.012	0.497HisTyr: 0.497 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.646IleAla: 4.646 ± 0.047	0.265IleCys: 0.265 ± 0.009	2.222IleAsp: 2.222 ± 0.029	1.913IleGlu: 1.913 ± 0.031	0.655IlePhe: 0.655 ± 0.016	3.479IleGly: 3.479 ± 0.042	0.588IleHis: 0.588 ± 0.014	0.86IleIle: 0.86 ± 0.02	0.734IleLys: 0.734 ± 0.018	2.42IleLeu: 2.42 ± 0.033	0.431IleMet: 0.431 ± 0.014	0.678IleAsn: 0.678 ± 0.015	1.766IlePro: 1.766 ± 0.026	0.743IleGln: 0.743 ± 0.019	2.157IleArg: 2.157 ± 0.029	1.647IleSer: 1.647 ± 0.025	2.268IleThr: 2.268 ± 0.031	2.714IleVal: 2.714 ± 0.032	0.377IleTrp: 0.377 ± 0.012	0.534IleTyr: 0.534 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	3.031LysAla: 3.031 ± 0.041	0.12LysCys: 0.12 ± 0.007	1.319LysAsp: 1.319 ± 0.023	1.172LysGlu: 1.172 ± 0.023	0.467LysPhe: 0.467 ± 0.015	1.845LysGly: 1.845 ± 0.032	0.411LysHis: 0.411 ± 0.012	0.887LysIle: 0.887 ± 0.019	0.86LysLys: 0.86 ± 0.025	1.976LysLeu: 1.976 ± 0.028	0.384LysMet: 0.384 ± 0.011	0.549LysAsn: 0.549 ± 0.014	1.302LysPro: 1.302 ± 0.021	0.72LysGln: 0.72 ± 0.019	1.393LysArg: 1.393 ± 0.025	1.212LysSer: 1.212 ± 0.026	1.35LysThr: 1.35 ± 0.025	1.932LysVal: 1.932 ± 0.038	0.28LysTrp: 0.28 ± 0.01	0.469LysTyr: 0.469 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	14.936LeuAla: 14.936 ± 0.106	0.834LeuCys: 0.834 ± 0.016	6.61LeuAsp: 6.61 ± 0.055	4.634LeuGlu: 4.634 ± 0.05	2.595LeuPhe: 2.595 ± 0.033	9.16LeuGly: 9.16 ± 0.072	2.31LeuHis: 2.31 ± 0.03	3.225LeuIle: 3.225 ± 0.037	2.073LeuLys: 2.073 ± 0.033	11.237LeuLeu: 11.237 ± 0.1	1.572LeuMet: 1.572 ± 0.023	1.706LeuAsn: 1.706 ± 0.022	6.464LeuPro: 6.464 ± 0.053	2.118LeuGln: 2.118 ± 0.028	8.572LeuArg: 8.572 ± 0.072	5.279LeuSer: 5.279 ± 0.043	7.081LeuThr: 7.081 ± 0.062	8.724LeuVal: 8.724 ± 0.073	1.319LeuTrp: 1.319 ± 0.024	1.92LeuTyr: 1.92 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	2.171MetAla: 2.171 ± 0.031	0.126MetCys: 0.126 ± 0.006	0.861MetAsp: 0.861 ± 0.017	0.711MetGlu: 0.711 ± 0.016	0.449MetPhe: 0.449 ± 0.013	1.279MetGly: 1.279 ± 0.027	0.338MetHis: 0.338 ± 0.011	0.658MetIle: 0.658 ± 0.017	0.411MetLys: 0.411 ± 0.011	1.557MetLeu: 1.557 ± 0.025	0.277MetMet: 0.277 ± 0.01	0.438MetAsn: 0.438 ± 0.013	1.082MetPro: 1.082 ± 0.018	0.443MetGln: 0.443 ± 0.013	1.361MetArg: 1.361 ± 0.023	1.347MetSer: 1.347 ± 0.023	1.552MetThr: 1.552 ± 0.025	1.22MetVal: 1.22 ± 0.02	0.214MetTrp: 0.214 ± 0.009	0.315MetTyr: 0.315 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.28AsnAla: 2.28 ± 0.033	0.173AsnCys: 0.173 ± 0.008	1.005AsnAsp: 1.005 ± 0.021	0.83AsnGlu: 0.83 ± 0.017	0.481AsnPhe: 0.481 ± 0.013	1.949AsnGly: 1.949 ± 0.032	0.404AsnHis: 0.404 ± 0.012	0.668AsnIle: 0.668 ± 0.015	0.433AsnLys: 0.433 ± 0.013	1.677AsnLeu: 1.677 ± 0.025	0.298AsnMet: 0.298 ± 0.01	0.464AsnAsn: 0.464 ± 0.017	1.383AsnPro: 1.383 ± 0.022	0.534AsnGln: 0.534 ± 0.017	1.273AsnArg: 1.273 ± 0.02	1.008AsnSer: 1.008 ± 0.024	1.182AsnThr: 1.182 ± 0.024	1.387AsnVal: 1.387 ± 0.023	0.306AsnTrp: 0.306 ± 0.009	0.428AsnTyr: 0.428 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	8.75ProAla: 8.75 ± 0.072	0.328ProCys: 0.328 ± 0.012	4.448ProAsp: 4.448 ± 0.045	4.21ProGlu: 4.21 ± 0.043	1.511ProPhe: 1.511 ± 0.025	6.613ProGly: 6.613 ± 0.063	1.439ProHis: 1.439 ± 0.023	1.261ProIle: 1.261 ± 0.023	1.173ProLys: 1.173 ± 0.022	5.337ProLeu: 5.337 ± 0.049	0.988ProMet: 0.988 ± 0.023	0.923ProAsn: 0.923 ± 0.018	3.604ProPro: 3.604 ± 0.055	1.806ProGln: 1.806 ± 0.032	4.056ProArg: 4.056 ± 0.045	3.35ProSer: 3.35 ± 0.042	3.414ProThr: 3.414 ± 0.038	5.497ProVal: 5.497 ± 0.05	0.902ProTrp: 0.902 ± 0.019	1.434ProTyr: 1.434 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.738GlnAla: 3.738 ± 0.039	0.178GlnCys: 0.178 ± 0.009	1.484GlnAsp: 1.484 ± 0.023	1.506GlnGlu: 1.506 ± 0.025	0.673GlnPhe: 0.673 ± 0.015	2.401GlnGly: 2.401 ± 0.033	0.681GlnHis: 0.681 ± 0.017	1.074GlnIle: 1.074 ± 0.02	0.63GlnLys: 0.63 ± 0.015	3.016GlnLeu: 3.016 ± 0.032	0.471GlnMet: 0.471 ± 0.012	0.545GlnAsn: 0.545 ± 0.017	1.703GlnPro: 1.703 ± 0.034	1.27GlnGln: 1.27 ± 0.032	2.321GlnArg: 2.321 ± 0.03	1.327GlnSer: 1.327 ± 0.022	1.384GlnThr: 1.384 ± 0.023	2.393GlnVal: 2.393 ± 0.03	0.491GlnTrp: 0.491 ± 0.011	0.628GlnTyr: 0.628 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	9.805ArgAla: 9.805 ± 0.073	0.591ArgCys: 0.591 ± 0.017	4.2ArgAsp: 4.2 ± 0.041	4.668ArgGlu: 4.668 ± 0.046	2.343ArgPhe: 2.343 ± 0.032	5.728ArgGly: 5.728 ± 0.045	2.104ArgHis: 2.104 ± 0.029	3.116ArgIle: 3.116 ± 0.034	1.595ArgLys: 1.595 ± 0.025	8.9ArgLeu: 8.9 ± 0.07	1.658ArgMet: 1.658 ± 0.022	1.34ArgAsn: 1.34 ± 0.025	5.091ArgPro: 5.091 ± 0.055	2.326ArgGln: 2.326 ± 0.031	7.768ArgArg: 7.768 ± 0.069	4.055ArgSer: 4.055 ± 0.041	5.419ArgThr: 5.419 ± 0.05	5.872ArgVal: 5.872 ± 0.05	1.357ArgTrp: 1.357 ± 0.023	1.8ArgTyr: 1.8 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	6.987SerAla: 6.987 ± 0.057	0.426SerCys: 0.426 ± 0.015	2.814SerAsp: 2.814 ± 0.032	2.447SerGlu: 2.447 ± 0.03	1.544SerPhe: 1.544 ± 0.025	6.187SerGly: 6.187 ± 0.058	1.006SerHis: 1.006 ± 0.016	1.407SerIle: 1.407 ± 0.024	1.076SerLys: 1.076 ± 0.023	4.812SerLeu: 4.812 ± 0.046	1.073SerMet: 1.073 ± 0.017	0.898SerAsn: 0.898 ± 0.018	3.273SerPro: 3.273 ± 0.038	1.288SerGln: 1.288 ± 0.018	3.706SerArg: 3.706 ± 0.04	3.084SerSer: 3.084 ± 0.044	3.326SerThr: 3.326 ± 0.039	4.385SerVal: 4.385 ± 0.04	0.943SerTrp: 0.943 ± 0.019	1.278SerTyr: 1.278 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	9.331ThrAla: 9.331 ± 0.064	0.463ThrCys: 0.463 ± 0.014	3.858ThrAsp: 3.858 ± 0.037	3.297ThrGlu: 3.297 ± 0.039	1.663ThrPhe: 1.663 ± 0.025	6.929ThrGly: 6.929 ± 0.064	1.253ThrHis: 1.253 ± 0.021	1.714ThrIle: 1.714 ± 0.026	1.245ThrLys: 1.245 ± 0.028	5.888ThrLeu: 5.888 ± 0.05	0.937ThrMet: 0.937 ± 0.017	1.086ThrAsn: 1.086 ± 0.023	4.226ThrPro: 4.226 ± 0.047	1.437ThrGln: 1.437 ± 0.022	4.05ThrArg: 4.05 ± 0.04	3.362ThrSer: 3.362 ± 0.042	4.187ThrThr: 4.187 ± 0.054	6.286ThrVal: 6.286 ± 0.055	0.925ThrTrp: 0.925 ± 0.018	1.441ThrTyr: 1.441 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	10.512ValAla: 10.512 ± 0.059	0.755ValCys: 0.755 ± 0.016	5.068ValAsp: 5.068 ± 0.046	4.684ValGlu: 4.684 ± 0.047	2.408ValPhe: 2.408 ± 0.033	6.61ValGly: 6.61 ± 0.05	1.98ValHis: 1.98 ± 0.027	2.751ValIle: 2.751 ± 0.029	1.779ValLys: 1.779 ± 0.031	9.405ValLeu: 9.405 ± 0.069	1.407ValMet: 1.407 ± 0.026	1.687ValAsn: 1.687 ± 0.029	5.289ValPro: 5.289 ± 0.049	2.148ValGln: 2.148 ± 0.029	7.175ValArg: 7.175 ± 0.054	4.389ValSer: 4.389 ± 0.043	5.856ValThr: 5.856 ± 0.05	8.006ValVal: 8.006 ± 0.067	1.199ValTrp: 1.199 ± 0.025	1.606ValTyr: 1.606 ± 0.025	0.0ValXaa: 0.0 ± 0.0
Trp	1.699TrpAla: 1.699 ± 0.024	0.157TrpCys: 0.157 ± 0.007	0.849TrpAsp: 0.849 ± 0.02	0.735TrpGlu: 0.735 ± 0.017	0.493TrpPhe: 0.493 ± 0.013	1.082TrpGly: 1.082 ± 0.02	0.383TrpHis: 0.383 ± 0.012	0.563TrpIle: 0.563 ± 0.014	0.393TrpLys: 0.393 ± 0.013	1.773TrpLeu: 1.773 ± 0.026	0.29TrpMet: 0.29 ± 0.01	0.455TrpAsn: 0.455 ± 0.014	0.79TrpPro: 0.79 ± 0.016	0.644TrpGln: 0.644 ± 0.016	1.341TrpArg: 1.341 ± 0.025	0.985TrpSer: 0.985 ± 0.022	1.096TrpThr: 1.096 ± 0.021	0.999TrpVal: 0.999 ± 0.019	0.341TrpTrp: 0.341 ± 0.01	0.372TrpTyr: 0.372 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.846TyrAla: 2.846 ± 0.031	0.177TyrCys: 0.177 ± 0.007	1.602TyrAsp: 1.602 ± 0.033	1.273TyrGlu: 1.273 ± 0.022	0.647TyrPhe: 0.647 ± 0.017	2.339TyrGly: 2.339 ± 0.035	0.401TyrHis: 0.401 ± 0.013	0.492TyrIle: 0.492 ± 0.013	0.413TyrLys: 0.413 ± 0.012	2.096TyrLeu: 2.096 ± 0.028	0.256TyrMet: 0.256 ± 0.01	0.438TyrAsn: 0.438 ± 0.013	1.096TyrPro: 1.096 ± 0.019	0.631TyrGln: 0.631 ± 0.016	1.835TyrArg: 1.835 ± 0.028	0.993TyrSer: 0.993 ± 0.022	1.258TyrThr: 1.258 ± 0.024	1.701TyrVal: 1.701 ± 0.025	0.372TyrTrp: 0.372 ± 0.012	0.487TyrTyr: 0.487 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9056 proteins (2938333 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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