Amino acid dipepetide frequency for Salmonella phage 22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.025AlaAla: 10.025 ± 1.117
0.935AlaCys: 0.935 ± 0.236
5.523AlaAsp: 5.523 ± 0.76
7.052AlaGlu: 7.052 ± 0.809
3.653AlaPhe: 3.653 ± 0.656
6.372AlaGly: 6.372 ± 0.944
1.444AlaHis: 1.444 ± 0.364
6.117AlaIle: 6.117 ± 0.83
5.353AlaLys: 5.353 ± 0.778
6.287AlaLeu: 6.287 ± 0.882
3.738AlaMet: 3.738 ± 0.556
5.862AlaAsn: 5.862 ± 0.906
2.464AlaPro: 2.464 ± 0.406
3.568AlaGln: 3.568 ± 0.523
5.777AlaArg: 5.777 ± 0.668
4.503AlaSer: 4.503 ± 0.563
5.692AlaThr: 5.692 ± 0.818
5.523AlaVal: 5.523 ± 0.596
1.614AlaTrp: 1.614 ± 0.318
2.719AlaTyr: 2.719 ± 0.486
0.0AlaXaa: 0.0 ± 0.0
Cys
0.85CysAla: 0.85 ± 0.279
0.255CysCys: 0.255 ± 0.162
0.34CysAsp: 0.34 ± 0.155
0.68CysGlu: 0.68 ± 0.234
0.595CysPhe: 0.595 ± 0.248
1.189CysGly: 1.189 ± 0.389
0.51CysHis: 0.51 ± 0.254
0.595CysIle: 0.595 ± 0.226
0.935CysLys: 0.935 ± 0.301
0.425CysLeu: 0.425 ± 0.168
0.34CysMet: 0.34 ± 0.185
0.765CysAsn: 0.765 ± 0.274
0.425CysPro: 0.425 ± 0.171
0.51CysGln: 0.51 ± 0.21
1.274CysArg: 1.274 ± 0.342
1.02CysSer: 1.02 ± 0.268
0.34CysThr: 0.34 ± 0.204
1.02CysVal: 1.02 ± 0.307
0.17CysTrp: 0.17 ± 0.12
0.595CysTyr: 0.595 ± 0.19
0.0CysXaa: 0.0 ± 0.0
Asp
7.392AspAla: 7.392 ± 0.976
0.765AspCys: 0.765 ± 0.292
3.653AspAsp: 3.653 ± 0.604
3.993AspGlu: 3.993 ± 0.593
2.039AspPhe: 2.039 ± 0.479
4.248AspGly: 4.248 ± 0.567
1.02AspHis: 1.02 ± 0.329
4.078AspIle: 4.078 ± 0.589
3.144AspLys: 3.144 ± 0.498
4.588AspLeu: 4.588 ± 0.719
1.869AspMet: 1.869 ± 0.365
1.954AspAsn: 1.954 ± 0.315
1.699AspPro: 1.699 ± 0.413
1.105AspGln: 1.105 ± 0.272
2.124AspArg: 2.124 ± 0.403
3.229AspSer: 3.229 ± 0.498
2.124AspThr: 2.124 ± 0.431
4.588AspVal: 4.588 ± 0.669
0.85AspTrp: 0.85 ± 0.288
3.483AspTyr: 3.483 ± 0.511
0.0AspXaa: 0.0 ± 0.0
Glu
5.692GluAla: 5.692 ± 0.7
1.529GluCys: 1.529 ± 0.345
3.738GluAsp: 3.738 ± 0.545
5.183GluGlu: 5.183 ± 0.865
2.039GluPhe: 2.039 ± 0.457
3.993GluGly: 3.993 ± 0.545
1.274GluHis: 1.274 ± 0.314
3.823GluIle: 3.823 ± 0.568
4.163GluLys: 4.163 ± 0.547
6.797GluLeu: 6.797 ± 0.773
2.039GluMet: 2.039 ± 0.482
3.144GluAsn: 3.144 ± 0.515
2.209GluPro: 2.209 ± 0.525
3.653GluGln: 3.653 ± 0.591
4.503GluArg: 4.503 ± 0.776
3.823GluSer: 3.823 ± 0.489
2.889GluThr: 2.889 ± 0.472
2.719GluVal: 2.719 ± 0.525
1.784GluTrp: 1.784 ± 0.363
1.784GluTyr: 1.784 ± 0.38
0.0GluXaa: 0.0 ± 0.0
Phe
2.974PheAla: 2.974 ± 0.494
0.765PheCys: 0.765 ± 0.275
2.039PheAsp: 2.039 ± 0.419
1.444PheGlu: 1.444 ± 0.33
1.105PhePhe: 1.105 ± 0.303
2.039PheGly: 2.039 ± 0.403
0.595PheHis: 0.595 ± 0.252
3.059PheIle: 3.059 ± 0.618
1.869PheLys: 1.869 ± 0.356
2.039PheLeu: 2.039 ± 0.408
1.105PheMet: 1.105 ± 0.284
2.209PheAsn: 2.209 ± 0.448
1.699PhePro: 1.699 ± 0.325
1.189PheGln: 1.189 ± 0.455
1.444PheArg: 1.444 ± 0.334
2.379PheSer: 2.379 ± 0.395
2.124PheThr: 2.124 ± 0.351
1.359PheVal: 1.359 ± 0.299
0.595PheTrp: 0.595 ± 0.207
1.02PheTyr: 1.02 ± 0.347
0.0PheXaa: 0.0 ± 0.0
Gly
5.268GlyAla: 5.268 ± 1.031
0.51GlyCys: 0.51 ± 0.188
3.398GlyAsp: 3.398 ± 0.472
3.908GlyGlu: 3.908 ± 0.606
2.464GlyPhe: 2.464 ± 0.459
4.418GlyGly: 4.418 ± 0.972
1.02GlyHis: 1.02 ± 0.279
4.333GlyIle: 4.333 ± 0.738
5.013GlyLys: 5.013 ± 0.649
5.183GlyLeu: 5.183 ± 0.829
2.719GlyMet: 2.719 ± 0.513
3.229GlyAsn: 3.229 ± 0.5
1.02GlyPro: 1.02 ± 0.269
3.993GlyGln: 3.993 ± 0.691
4.503GlyArg: 4.503 ± 0.773
3.823GlySer: 3.823 ± 0.619
3.738GlyThr: 3.738 ± 0.566
5.183GlyVal: 5.183 ± 0.658
1.444GlyTrp: 1.444 ± 0.301
2.209GlyTyr: 2.209 ± 0.39
0.0GlyXaa: 0.0 ± 0.0
His
1.189HisAla: 1.189 ± 0.361
0.255HisCys: 0.255 ± 0.151
1.189HisAsp: 1.189 ± 0.357
1.784HisGlu: 1.784 ± 0.395
1.02HisPhe: 1.02 ± 0.314
0.85HisGly: 0.85 ± 0.289
0.255HisHis: 0.255 ± 0.153
0.765HisIle: 0.765 ± 0.207
1.444HisLys: 1.444 ± 0.426
1.529HisLeu: 1.529 ± 0.387
0.595HisMet: 0.595 ± 0.199
0.425HisAsn: 0.425 ± 0.19
0.85HisPro: 0.85 ± 0.282
0.935HisGln: 0.935 ± 0.332
0.935HisArg: 0.935 ± 0.269
0.85HisSer: 0.85 ± 0.262
0.595HisThr: 0.595 ± 0.229
0.765HisVal: 0.765 ± 0.258
0.255HisTrp: 0.255 ± 0.136
0.68HisTyr: 0.68 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
6.627IleAla: 6.627 ± 0.814
0.595IleCys: 0.595 ± 0.232
3.653IleAsp: 3.653 ± 0.522
3.993IleGlu: 3.993 ± 0.537
1.699IlePhe: 1.699 ± 0.412
5.692IleGly: 5.692 ± 0.996
1.274IleHis: 1.274 ± 0.317
4.758IleIle: 4.758 ± 0.826
3.314IleLys: 3.314 ± 0.401
3.483IleLeu: 3.483 ± 0.573
1.359IleMet: 1.359 ± 0.358
3.059IleAsn: 3.059 ± 0.53
3.059IlePro: 3.059 ± 0.394
1.784IleGln: 1.784 ± 0.356
3.398IleArg: 3.398 ± 0.517
4.673IleSer: 4.673 ± 0.753
5.268IleThr: 5.268 ± 0.525
3.229IleVal: 3.229 ± 0.574
0.51IleTrp: 0.51 ± 0.171
2.124IleTyr: 2.124 ± 0.379
0.0IleXaa: 0.0 ± 0.0
Lys
5.438LysAla: 5.438 ± 0.837
0.85LysCys: 0.85 ± 0.25
3.144LysAsp: 3.144 ± 0.435
4.673LysGlu: 4.673 ± 0.613
1.869LysPhe: 1.869 ± 0.384
3.653LysGly: 3.653 ± 0.495
0.85LysHis: 0.85 ± 0.211
3.314LysIle: 3.314 ± 0.52
4.758LysLys: 4.758 ± 0.69
6.457LysLeu: 6.457 ± 0.857
1.359LysMet: 1.359 ± 0.294
2.124LysAsn: 2.124 ± 0.373
4.503LysPro: 4.503 ± 0.581
3.314LysGln: 3.314 ± 0.701
4.418LysArg: 4.418 ± 0.605
3.908LysSer: 3.908 ± 0.654
3.738LysThr: 3.738 ± 0.57
2.889LysVal: 2.889 ± 0.511
0.255LysTrp: 0.255 ± 0.154
2.209LysTyr: 2.209 ± 0.456
0.0LysXaa: 0.0 ± 0.0
Leu
7.562LeuAla: 7.562 ± 1.028
0.595LeuCys: 0.595 ± 0.241
3.398LeuAsp: 3.398 ± 0.553
5.183LeuGlu: 5.183 ± 0.739
2.719LeuPhe: 2.719 ± 0.56
4.333LeuGly: 4.333 ± 0.712
1.274LeuHis: 1.274 ± 0.337
5.013LeuIle: 5.013 ± 0.689
6.372LeuLys: 6.372 ± 0.86
6.797LeuLeu: 6.797 ± 0.671
2.804LeuMet: 2.804 ± 0.385
4.418LeuAsn: 4.418 ± 0.654
2.974LeuPro: 2.974 ± 0.53
2.719LeuGln: 2.719 ± 0.491
4.588LeuArg: 4.588 ± 0.699
5.607LeuSer: 5.607 ± 0.55
4.503LeuThr: 4.503 ± 0.583
4.333LeuVal: 4.333 ± 0.582
0.765LeuTrp: 0.765 ± 0.27
2.124LeuTyr: 2.124 ± 0.408
0.0LeuXaa: 0.0 ± 0.0
Met
3.398MetAla: 3.398 ± 0.512
0.255MetCys: 0.255 ± 0.14
1.444MetAsp: 1.444 ± 0.351
1.529MetGlu: 1.529 ± 0.351
0.51MetPhe: 0.51 ± 0.234
1.954MetGly: 1.954 ± 0.425
0.085MetHis: 0.085 ± 0.085
1.614MetIle: 1.614 ± 0.334
2.719MetLys: 2.719 ± 0.567
2.464MetLeu: 2.464 ± 0.418
1.02MetMet: 1.02 ± 0.31
1.189MetAsn: 1.189 ± 0.342
1.274MetPro: 1.274 ± 0.361
2.039MetGln: 2.039 ± 0.413
2.634MetArg: 2.634 ± 0.533
2.974MetSer: 2.974 ± 0.452
1.784MetThr: 1.784 ± 0.427
1.954MetVal: 1.954 ± 0.361
0.085MetTrp: 0.085 ± 0.083
1.02MetTyr: 1.02 ± 0.291
0.0MetXaa: 0.0 ± 0.0
Asn
5.268AsnAla: 5.268 ± 0.744
0.34AsnCys: 0.34 ± 0.178
2.209AsnAsp: 2.209 ± 0.468
2.549AsnGlu: 2.549 ± 0.423
0.51AsnPhe: 0.51 ± 0.193
4.333AsnGly: 4.333 ± 0.488
1.105AsnHis: 1.105 ± 0.348
3.398AsnIle: 3.398 ± 0.548
2.549AsnLys: 2.549 ± 0.486
2.974AsnLeu: 2.974 ± 0.53
1.529AsnMet: 1.529 ± 0.328
2.634AsnAsn: 2.634 ± 0.533
2.464AsnPro: 2.464 ± 0.43
2.634AsnGln: 2.634 ± 0.504
2.209AsnArg: 2.209 ± 0.475
3.314AsnSer: 3.314 ± 0.518
2.549AsnThr: 2.549 ± 0.572
2.719AsnVal: 2.719 ± 0.678
0.51AsnTrp: 0.51 ± 0.212
1.359AsnTyr: 1.359 ± 0.36
0.0AsnXaa: 0.0 ± 0.0
Pro
2.719ProAla: 2.719 ± 0.36
0.255ProCys: 0.255 ± 0.138
3.314ProAsp: 3.314 ± 0.496
4.758ProGlu: 4.758 ± 0.623
1.274ProPhe: 1.274 ± 0.291
2.039ProGly: 2.039 ± 0.466
0.595ProHis: 0.595 ± 0.248
2.379ProIle: 2.379 ± 0.353
2.379ProLys: 2.379 ± 0.361
3.229ProLeu: 3.229 ± 0.606
0.85ProMet: 0.85 ± 0.269
1.784ProAsn: 1.784 ± 0.337
1.614ProPro: 1.614 ± 0.376
1.699ProGln: 1.699 ± 0.278
1.869ProArg: 1.869 ± 0.331
2.464ProSer: 2.464 ± 0.341
2.039ProThr: 2.039 ± 0.396
2.889ProVal: 2.889 ± 0.429
0.34ProTrp: 0.34 ± 0.203
1.02ProTyr: 1.02 ± 0.334
0.0ProXaa: 0.0 ± 0.0
Gln
3.398GlnAla: 3.398 ± 0.514
0.51GlnCys: 0.51 ± 0.215
2.804GlnAsp: 2.804 ± 0.54
2.464GlnGlu: 2.464 ± 0.443
1.105GlnPhe: 1.105 ± 0.278
2.719GlnGly: 2.719 ± 0.564
0.935GlnHis: 0.935 ± 0.255
2.974GlnIle: 2.974 ± 0.545
1.954GlnLys: 1.954 ± 0.485
3.823GlnLeu: 3.823 ± 0.545
1.529GlnMet: 1.529 ± 0.257
1.954GlnAsn: 1.954 ± 0.649
1.869GlnPro: 1.869 ± 0.36
3.483GlnGln: 3.483 ± 0.716
2.974GlnArg: 2.974 ± 0.461
3.653GlnSer: 3.653 ± 0.572
1.699GlnThr: 1.699 ± 0.364
2.379GlnVal: 2.379 ± 0.453
1.02GlnTrp: 1.02 ± 0.352
1.869GlnTyr: 1.869 ± 0.423
0.0GlnXaa: 0.0 ± 0.0
Arg
4.758ArgAla: 4.758 ± 0.572
0.68ArgCys: 0.68 ± 0.275
3.738ArgAsp: 3.738 ± 0.663
4.163ArgGlu: 4.163 ± 0.562
1.869ArgPhe: 1.869 ± 0.413
3.398ArgGly: 3.398 ± 0.629
1.444ArgHis: 1.444 ± 0.303
3.398ArgIle: 3.398 ± 0.586
4.333ArgLys: 4.333 ± 0.689
4.843ArgLeu: 4.843 ± 0.583
2.719ArgMet: 2.719 ± 0.482
2.719ArgAsn: 2.719 ± 0.665
1.954ArgPro: 1.954 ± 0.456
2.464ArgGln: 2.464 ± 0.46
3.738ArgArg: 3.738 ± 0.752
3.653ArgSer: 3.653 ± 0.552
1.954ArgThr: 1.954 ± 0.37
3.738ArgVal: 3.738 ± 0.51
0.85ArgTrp: 0.85 ± 0.248
2.124ArgTyr: 2.124 ± 0.499
0.0ArgXaa: 0.0 ± 0.0
Ser
5.268SerAla: 5.268 ± 0.64
1.105SerCys: 1.105 ± 0.303
3.738SerAsp: 3.738 ± 0.512
3.738SerGlu: 3.738 ± 0.491
2.634SerPhe: 2.634 ± 0.465
5.777SerGly: 5.777 ± 0.878
1.274SerHis: 1.274 ± 0.335
4.418SerIle: 4.418 ± 0.664
3.144SerLys: 3.144 ± 0.38
5.607SerLeu: 5.607 ± 0.713
2.039SerMet: 2.039 ± 0.405
2.549SerAsn: 2.549 ± 0.409
2.804SerPro: 2.804 ± 0.438
2.804SerGln: 2.804 ± 0.566
3.398SerArg: 3.398 ± 0.571
3.483SerSer: 3.483 ± 0.78
2.889SerThr: 2.889 ± 0.565
3.738SerVal: 3.738 ± 0.509
0.85SerTrp: 0.85 ± 0.289
2.379SerTyr: 2.379 ± 0.523
0.0SerXaa: 0.0 ± 0.0
Thr
6.202ThrAla: 6.202 ± 0.614
0.765ThrCys: 0.765 ± 0.235
3.059ThrAsp: 3.059 ± 0.548
2.889ThrGlu: 2.889 ± 0.505
1.784ThrPhe: 1.784 ± 0.441
3.993ThrGly: 3.993 ± 0.477
0.68ThrHis: 0.68 ± 0.206
3.059ThrIle: 3.059 ± 0.487
3.993ThrLys: 3.993 ± 0.714
3.738ThrLeu: 3.738 ± 0.663
1.02ThrMet: 1.02 ± 0.333
1.954ThrAsn: 1.954 ± 0.43
2.719ThrPro: 2.719 ± 0.526
2.549ThrGln: 2.549 ± 0.481
2.634ThrArg: 2.634 ± 0.492
3.229ThrSer: 3.229 ± 0.553
3.144ThrThr: 3.144 ± 0.497
2.974ThrVal: 2.974 ± 0.421
0.68ThrTrp: 0.68 ± 0.203
1.699ThrTyr: 1.699 ± 0.345
0.0ThrXaa: 0.0 ± 0.0
Val
6.372ValAla: 6.372 ± 0.597
0.765ValCys: 0.765 ± 0.264
3.398ValAsp: 3.398 ± 0.538
4.503ValGlu: 4.503 ± 0.551
2.124ValPhe: 2.124 ± 0.434
3.653ValGly: 3.653 ± 0.639
0.425ValHis: 0.425 ± 0.175
3.314ValIle: 3.314 ± 0.59
4.078ValLys: 4.078 ± 0.513
4.418ValLeu: 4.418 ± 0.678
1.614ValMet: 1.614 ± 0.394
3.059ValAsn: 3.059 ± 0.534
1.954ValPro: 1.954 ± 0.381
2.039ValGln: 2.039 ± 0.369
3.229ValArg: 3.229 ± 0.456
3.993ValSer: 3.993 ± 0.567
3.398ValThr: 3.398 ± 0.553
4.503ValVal: 4.503 ± 0.581
0.68ValTrp: 0.68 ± 0.244
1.699ValTyr: 1.699 ± 0.507
0.0ValXaa: 0.0 ± 0.0
Trp
0.935TrpAla: 0.935 ± 0.279
0.34TrpCys: 0.34 ± 0.171
0.935TrpAsp: 0.935 ± 0.239
0.765TrpGlu: 0.765 ± 0.286
0.51TrpPhe: 0.51 ± 0.207
0.85TrpGly: 0.85 ± 0.251
0.255TrpHis: 0.255 ± 0.156
0.765TrpIle: 0.765 ± 0.28
1.105TrpLys: 1.105 ± 0.364
1.274TrpLeu: 1.274 ± 0.372
0.85TrpMet: 0.85 ± 0.266
0.425TrpAsn: 0.425 ± 0.197
0.51TrpPro: 0.51 ± 0.182
0.85TrpGln: 0.85 ± 0.249
0.68TrpArg: 0.68 ± 0.218
0.85TrpSer: 0.85 ± 0.256
0.595TrpThr: 0.595 ± 0.189
0.85TrpVal: 0.85 ± 0.257
0.255TrpTrp: 0.255 ± 0.156
0.34TrpTyr: 0.34 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.974TyrAla: 2.974 ± 0.541
0.765TyrCys: 0.765 ± 0.254
3.229TyrAsp: 3.229 ± 0.675
1.529TyrGlu: 1.529 ± 0.456
1.529TyrPhe: 1.529 ± 0.379
1.869TyrGly: 1.869 ± 0.478
0.85TyrHis: 0.85 ± 0.308
2.379TyrIle: 2.379 ± 0.566
1.105TyrLys: 1.105 ± 0.274
2.124TyrLeu: 2.124 ± 0.339
0.765TyrMet: 0.765 ± 0.215
1.699TyrAsn: 1.699 ± 0.403
1.444TyrPro: 1.444 ± 0.369
1.784TyrGln: 1.784 ± 0.476
2.209TyrArg: 2.209 ± 0.478
2.209TyrSer: 2.209 ± 0.488
1.699TyrThr: 1.699 ± 0.336
1.869TyrVal: 1.869 ± 0.348
0.425TyrTrp: 0.425 ± 0.192
1.02TyrTyr: 1.02 ± 0.341
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (11771 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski