Bacillus phage BtiUFT6.51-F

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Camtrevirus; unclassified Camtrevirus

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6UF39|A0A2I6UF39_9CAUD Uncharacterized protein OS=Bacillus phage BtiUFT6.51-F OX=2069313 GN=XO27_0023 PE=4 SV=1
MM1 pKa = 7.58GLNGLIGGIEE11 pKa = 3.88MTKK14 pKa = 10.67EE15 pKa = 3.82EE16 pKa = 4.28IVEE19 pKa = 4.19LFLNTVDD26 pKa = 3.52EE27 pKa = 5.02AKK29 pKa = 10.57PEE31 pKa = 4.56LIEE34 pKa = 4.27EE35 pKa = 4.44YY36 pKa = 9.96IEE38 pKa = 3.89EE39 pKa = 4.2SFRR42 pKa = 11.84II43 pKa = 4.0

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6UF37|A0A2I6UF37_9CAUD DNA-binding helix-turn-helix protein OS=Bacillus phage BtiUFT6.51-F OX=2069313 GN=XO27_0036 PE=4 SV=1
MM1 pKa = 7.4VIFWKK6 pKa = 10.54HH7 pKa = 6.1DD8 pKa = 3.29ILRR11 pKa = 11.84LTHH14 pKa = 6.66TGQLFPKK21 pKa = 8.76RR22 pKa = 11.84WLGKK26 pKa = 7.77RR27 pKa = 11.84CKK29 pKa = 10.23RR30 pKa = 11.84NFQLAPLTHH39 pKa = 6.91ASMGLYY45 pKa = 9.66LCSIIIFQFDD55 pKa = 3.57KK56 pKa = 11.13PLVLLSLSS64 pKa = 3.54

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

11944

28

1341

189.6

21.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.355 ± 0.569

0.887 ± 0.173

5.417 ± 0.248

8.255 ± 0.308

4.203 ± 0.274

5.509 ± 0.367

1.783 ± 0.182

7.05 ± 0.256

9.503 ± 0.259

8.163 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.039 ± 0.206

5.635 ± 0.213

2.746 ± 0.23

4.488 ± 0.268

4.605 ± 0.203

5.702 ± 0.198

5.626 ± 0.354

6.162 ± 0.247

1.222 ± 0.117

3.65 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski