Streptococcus satellite phage Javan296

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJ50|A0A4D5ZJ50_9VIRU Repressor OS=Streptococcus satellite phage Javan296 OX=2558615 GN=JavanS296_0015 PE=4 SV=1
MM1 pKa = 6.88TLKK4 pKa = 10.04TFSDD8 pKa = 3.49KK9 pKa = 11.46AKK11 pKa = 9.54TFTFTYY17 pKa = 9.87EE18 pKa = 4.22FKK20 pKa = 11.13DD21 pKa = 3.28LDD23 pKa = 3.75TAMVAGHH30 pKa = 7.15ALLGYY35 pKa = 7.54MTGTYY40 pKa = 9.01CQPTISLTHH49 pKa = 6.59KK50 pKa = 10.97DD51 pKa = 3.12KK52 pKa = 10.58GTLVAEE58 pKa = 4.24YY59 pKa = 11.19VEE61 pKa = 4.59DD62 pKa = 3.69SKK64 pKa = 11.88LNKK67 pKa = 8.21TFKK70 pKa = 10.49RR71 pKa = 11.84ICDD74 pKa = 3.7SFKK77 pKa = 10.87DD78 pKa = 4.26YY79 pKa = 11.51YY80 pKa = 9.82NQPVDD85 pKa = 4.45DD86 pKa = 4.12EE87 pKa = 4.7AFEE90 pKa = 4.06EE91 pKa = 4.93RR92 pKa = 11.84YY93 pKa = 9.8KK94 pKa = 10.7RR95 pKa = 11.84EE96 pKa = 3.48RR97 pKa = 11.84VLQLKK102 pKa = 9.9EE103 pKa = 4.29SEE105 pKa = 4.49DD106 pKa = 4.18FEE108 pKa = 5.24SLLNKK113 pKa = 8.93ATDD116 pKa = 3.78YY117 pKa = 11.09EE118 pKa = 4.87LEE120 pKa = 4.21LLDD123 pKa = 3.9YY124 pKa = 11.08ADD126 pKa = 5.31RR127 pKa = 11.84LLSDD131 pKa = 4.21KK132 pKa = 9.93PIPMDD137 pKa = 3.3SMTAFGTLEE146 pKa = 3.95KK147 pKa = 10.88LGDD150 pKa = 3.75EE151 pKa = 5.13SISLLQKK158 pKa = 10.52LDD160 pKa = 3.32VEE162 pKa = 4.81GEE164 pKa = 4.36YY165 pKa = 10.5KK166 pKa = 10.76GLADD170 pKa = 3.67YY171 pKa = 10.39TEE173 pKa = 4.23HH174 pKa = 7.28LKK176 pKa = 10.96

Molecular weight: 20.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZNQ9|A0A4D5ZNQ9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan296 OX=2558615 GN=JavanS296_0014 PE=4 SV=1
MM1 pKa = 7.65FDD3 pKa = 4.52GFPEE7 pKa = 3.82IPRR10 pKa = 11.84YY11 pKa = 8.1MKK13 pKa = 9.66RR14 pKa = 11.84DD15 pKa = 3.69RR16 pKa = 11.84YY17 pKa = 9.2HH18 pKa = 5.94KK19 pKa = 10.09HH20 pKa = 4.58YY21 pKa = 10.97KK22 pKa = 10.24KK23 pKa = 10.13FLKK26 pKa = 10.6YY27 pKa = 9.68IEE29 pKa = 4.58KK30 pKa = 10.6GDD32 pKa = 3.96RR33 pKa = 11.84FWLNGLRR40 pKa = 11.84LL41 pKa = 3.6

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

3031

41

516

168.4

19.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.576 ± 0.383

0.495 ± 0.108

5.279 ± 0.47

9.337 ± 0.725

4.487 ± 0.302

4.619 ± 0.34

1.914 ± 0.289

7.093 ± 0.728

9.535 ± 0.407

9.766 ± 0.529

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.375 ± 0.333

5.444 ± 0.472

3.233 ± 0.585

3.695 ± 0.328

5.477 ± 0.361

5.444 ± 0.392

6.137 ± 0.343

4.982 ± 0.329

0.693 ± 0.177

4.421 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski