Amino acid dipepetide frequency for Mycobacterium phage Curiosium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.59AlaAla: 19.59 ± 1.316
1.34AlaCys: 1.34 ± 0.34
8.815AlaAsp: 8.815 ± 0.678
8.661AlaGlu: 8.661 ± 0.933
2.887AlaPhe: 2.887 ± 0.625
8.815AlaGly: 8.815 ± 0.954
2.99AlaHis: 2.99 ± 0.383
5.722AlaIle: 5.722 ± 0.654
3.196AlaLys: 3.196 ± 0.441
12.012AlaLeu: 12.012 ± 1.008
3.506AlaMet: 3.506 ± 0.405
2.732AlaAsn: 2.732 ± 0.461
7.32AlaPro: 7.32 ± 0.748
4.537AlaGln: 4.537 ± 0.611
8.403AlaArg: 8.403 ± 0.714
4.691AlaSer: 4.691 ± 0.658
6.186AlaThr: 6.186 ± 0.723
9.795AlaVal: 9.795 ± 0.85
2.062AlaTrp: 2.062 ± 0.348
2.835AlaTyr: 2.835 ± 0.395
0.0AlaXaa: 0.0 ± 0.0
Cys
1.186CysAla: 1.186 ± 0.272
0.206CysCys: 0.206 ± 0.104
0.722CysAsp: 0.722 ± 0.218
0.567CysGlu: 0.567 ± 0.198
0.155CysPhe: 0.155 ± 0.089
1.804CysGly: 1.804 ± 0.382
0.155CysHis: 0.155 ± 0.099
0.361CysIle: 0.361 ± 0.12
0.258CysLys: 0.258 ± 0.103
0.722CysLeu: 0.722 ± 0.203
0.155CysMet: 0.155 ± 0.089
0.309CysAsn: 0.309 ± 0.115
0.825CysPro: 0.825 ± 0.263
0.464CysGln: 0.464 ± 0.182
1.083CysArg: 1.083 ± 0.253
0.619CysSer: 0.619 ± 0.207
0.67CysThr: 0.67 ± 0.223
1.031CysVal: 1.031 ± 0.229
0.567CysTrp: 0.567 ± 0.159
0.258CysTyr: 0.258 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
7.423AspAla: 7.423 ± 0.885
0.722AspCys: 0.722 ± 0.235
5.928AspAsp: 5.928 ± 0.711
5.619AspGlu: 5.619 ± 0.646
1.186AspPhe: 1.186 ± 0.203
7.32AspGly: 7.32 ± 0.679
0.67AspHis: 0.67 ± 0.202
1.753AspIle: 1.753 ± 0.317
1.959AspLys: 1.959 ± 0.385
6.135AspLeu: 6.135 ± 0.687
1.495AspMet: 1.495 ± 0.286
1.856AspAsn: 1.856 ± 0.268
3.969AspPro: 3.969 ± 0.488
2.526AspGln: 2.526 ± 0.444
4.949AspArg: 4.949 ± 0.623
2.629AspSer: 2.629 ± 0.358
3.351AspThr: 3.351 ± 0.329
3.969AspVal: 3.969 ± 0.427
1.186AspTrp: 1.186 ± 0.246
1.443AspTyr: 1.443 ± 0.254
0.0AspXaa: 0.0 ± 0.0
Glu
7.527GluAla: 7.527 ± 0.765
0.928GluCys: 0.928 ± 0.242
2.784GluAsp: 2.784 ± 0.452
1.289GluGlu: 1.289 ± 0.297
2.062GluPhe: 2.062 ± 0.334
3.815GluGly: 3.815 ± 0.494
1.34GluHis: 1.34 ± 0.329
2.474GluIle: 2.474 ± 0.407
1.392GluLys: 1.392 ± 0.359
6.341GluLeu: 6.341 ± 0.595
1.907GluMet: 1.907 ± 0.287
1.237GluAsn: 1.237 ± 0.283
3.093GluPro: 3.093 ± 0.476
2.784GluGln: 2.784 ± 0.38
5.052GluArg: 5.052 ± 0.574
2.371GluSer: 2.371 ± 0.326
2.887GluThr: 2.887 ± 0.327
5.413GluVal: 5.413 ± 0.637
1.237GluTrp: 1.237 ± 0.245
1.804GluTyr: 1.804 ± 0.25
0.0GluXaa: 0.0 ± 0.0
Phe
2.938PheAla: 2.938 ± 0.577
0.258PheCys: 0.258 ± 0.116
3.145PheAsp: 3.145 ± 0.42
1.598PheGlu: 1.598 ± 0.284
0.619PhePhe: 0.619 ± 0.195
2.835PheGly: 2.835 ± 0.361
0.309PheHis: 0.309 ± 0.139
1.083PheIle: 1.083 ± 0.251
0.928PheLys: 0.928 ± 0.195
1.392PheLeu: 1.392 ± 0.252
0.619PheMet: 0.619 ± 0.163
1.186PheAsn: 1.186 ± 0.317
0.876PhePro: 0.876 ± 0.21
0.722PheGln: 0.722 ± 0.2
1.65PheArg: 1.65 ± 0.35
1.134PheSer: 1.134 ± 0.314
1.495PheThr: 1.495 ± 0.362
1.856PheVal: 1.856 ± 0.308
0.361PheTrp: 0.361 ± 0.133
0.516PheTyr: 0.516 ± 0.209
0.0PheXaa: 0.0 ± 0.0
Gly
11.548GlyAla: 11.548 ± 0.903
1.186GlyCys: 1.186 ± 0.318
5.671GlyAsp: 5.671 ± 0.563
5.464GlyGlu: 5.464 ± 0.563
2.217GlyPhe: 2.217 ± 0.37
9.228GlyGly: 9.228 ± 1.488
1.804GlyHis: 1.804 ± 0.31
2.732GlyIle: 2.732 ± 0.51
3.454GlyLys: 3.454 ± 0.465
7.32GlyLeu: 7.32 ± 0.779
2.062GlyMet: 2.062 ± 0.29
2.938GlyAsn: 2.938 ± 0.357
3.351GlyPro: 3.351 ± 0.555
2.835GlyGln: 2.835 ± 0.423
5.155GlyArg: 5.155 ± 0.504
5.464GlySer: 5.464 ± 0.858
5.619GlyThr: 5.619 ± 0.583
7.32GlyVal: 7.32 ± 0.575
2.268GlyTrp: 2.268 ± 0.299
2.217GlyTyr: 2.217 ± 0.5
0.0GlyXaa: 0.0 ± 0.0
His
2.423HisAla: 2.423 ± 0.399
0.258HisCys: 0.258 ± 0.099
1.598HisAsp: 1.598 ± 0.353
1.134HisGlu: 1.134 ± 0.247
0.412HisPhe: 0.412 ± 0.155
2.423HisGly: 2.423 ± 0.346
0.361HisHis: 0.361 ± 0.144
0.516HisIle: 0.516 ± 0.173
0.567HisLys: 0.567 ± 0.173
1.804HisLeu: 1.804 ± 0.339
0.206HisMet: 0.206 ± 0.1
0.412HisAsn: 0.412 ± 0.139
1.495HisPro: 1.495 ± 0.269
0.361HisGln: 0.361 ± 0.146
1.598HisArg: 1.598 ± 0.312
0.722HisSer: 0.722 ± 0.206
1.65HisThr: 1.65 ± 0.27
2.32HisVal: 2.32 ± 0.352
0.412HisTrp: 0.412 ± 0.182
0.67HisTyr: 0.67 ± 0.183
0.0HisXaa: 0.0 ± 0.0
Ile
5.361IleAla: 5.361 ± 0.463
0.052IleCys: 0.052 ± 0.056
3.248IleAsp: 3.248 ± 0.433
3.299IleGlu: 3.299 ± 0.475
0.825IlePhe: 0.825 ± 0.194
4.537IleGly: 4.537 ± 0.76
0.361IleHis: 0.361 ± 0.199
0.619IleIle: 0.619 ± 0.225
1.289IleLys: 1.289 ± 0.21
3.248IleLeu: 3.248 ± 0.368
0.155IleMet: 0.155 ± 0.078
1.186IleAsn: 1.186 ± 0.232
2.062IlePro: 2.062 ± 0.456
0.876IleGln: 0.876 ± 0.232
2.474IleArg: 2.474 ± 0.374
1.34IleSer: 1.34 ± 0.224
2.99IleThr: 2.99 ± 0.402
3.609IleVal: 3.609 ± 0.478
0.67IleTrp: 0.67 ± 0.174
0.619IleTyr: 0.619 ± 0.187
0.0IleXaa: 0.0 ± 0.0
Lys
3.712LysAla: 3.712 ± 0.445
0.464LysCys: 0.464 ± 0.138
1.237LysAsp: 1.237 ± 0.228
0.567LysGlu: 0.567 ± 0.156
0.876LysPhe: 0.876 ± 0.275
2.938LysGly: 2.938 ± 0.615
0.516LysHis: 0.516 ± 0.135
1.65LysIle: 1.65 ± 0.366
0.516LysLys: 0.516 ± 0.198
2.887LysLeu: 2.887 ± 0.425
1.186LysMet: 1.186 ± 0.281
0.619LysAsn: 0.619 ± 0.141
2.217LysPro: 2.217 ± 0.415
0.516LysGln: 0.516 ± 0.159
2.835LysArg: 2.835 ± 0.42
1.186LysSer: 1.186 ± 0.279
1.598LysThr: 1.598 ± 0.343
2.681LysVal: 2.681 ± 0.393
0.67LysTrp: 0.67 ± 0.161
1.031LysTyr: 1.031 ± 0.267
0.0LysXaa: 0.0 ± 0.0
Leu
11.651LeuAla: 11.651 ± 0.971
1.031LeuCys: 1.031 ± 0.222
7.011LeuAsp: 7.011 ± 0.61
2.887LeuGlu: 2.887 ± 0.394
1.804LeuPhe: 1.804 ± 0.304
7.733LeuGly: 7.733 ± 0.701
2.114LeuHis: 2.114 ± 0.346
3.351LeuIle: 3.351 ± 0.501
2.578LeuLys: 2.578 ± 0.343
5.774LeuLeu: 5.774 ± 0.622
1.65LeuMet: 1.65 ± 0.25
2.062LeuAsn: 2.062 ± 0.306
4.124LeuPro: 4.124 ± 0.522
2.938LeuGln: 2.938 ± 0.389
6.289LeuArg: 6.289 ± 0.597
4.897LeuSer: 4.897 ± 0.383
5.877LeuThr: 5.877 ± 0.628
6.341LeuVal: 6.341 ± 0.455
1.598LeuTrp: 1.598 ± 0.251
1.598LeuTyr: 1.598 ± 0.277
0.0LeuXaa: 0.0 ± 0.0
Met
2.732MetAla: 2.732 ± 0.413
0.052MetCys: 0.052 ± 0.058
0.722MetAsp: 0.722 ± 0.172
0.773MetGlu: 0.773 ± 0.201
0.979MetPhe: 0.979 ± 0.216
1.443MetGly: 1.443 ± 0.325
0.567MetHis: 0.567 ± 0.185
0.928MetIle: 0.928 ± 0.219
0.876MetLys: 0.876 ± 0.23
1.804MetLeu: 1.804 ± 0.275
0.773MetMet: 0.773 ± 0.19
0.722MetAsn: 0.722 ± 0.199
1.031MetPro: 1.031 ± 0.266
0.825MetGln: 0.825 ± 0.211
1.598MetArg: 1.598 ± 0.29
2.887MetSer: 2.887 ± 0.332
2.114MetThr: 2.114 ± 0.297
1.495MetVal: 1.495 ± 0.27
0.464MetTrp: 0.464 ± 0.149
0.516MetTyr: 0.516 ± 0.183
0.0MetXaa: 0.0 ± 0.0
Asn
3.299AsnAla: 3.299 ± 0.533
0.361AsnCys: 0.361 ± 0.144
1.186AsnAsp: 1.186 ± 0.28
1.443AsnGlu: 1.443 ± 0.27
0.825AsnPhe: 0.825 ± 0.246
3.815AsnGly: 3.815 ± 0.428
0.67AsnHis: 0.67 ± 0.172
0.825AsnIle: 0.825 ± 0.227
0.773AsnLys: 0.773 ± 0.226
1.907AsnLeu: 1.907 ± 0.265
0.361AsnMet: 0.361 ± 0.133
0.67AsnAsn: 0.67 ± 0.257
2.32AsnPro: 2.32 ± 0.33
0.722AsnGln: 0.722 ± 0.172
1.443AsnArg: 1.443 ± 0.343
1.289AsnSer: 1.289 ± 0.26
1.907AsnThr: 1.907 ± 0.358
1.959AsnVal: 1.959 ± 0.24
0.619AsnTrp: 0.619 ± 0.166
0.309AsnTyr: 0.309 ± 0.101
0.0AsnXaa: 0.0 ± 0.0
Pro
6.856ProAla: 6.856 ± 0.636
0.361ProCys: 0.361 ± 0.133
3.145ProAsp: 3.145 ± 0.408
3.969ProGlu: 3.969 ± 0.577
1.547ProPhe: 1.547 ± 0.282
5.361ProGly: 5.361 ± 0.564
1.237ProHis: 1.237 ± 0.27
1.907ProIle: 1.907 ± 0.321
1.959ProLys: 1.959 ± 0.473
3.918ProLeu: 3.918 ± 0.406
1.237ProMet: 1.237 ± 0.264
1.134ProAsn: 1.134 ± 0.254
3.145ProPro: 3.145 ± 0.482
1.753ProGln: 1.753 ± 0.338
3.918ProArg: 3.918 ± 0.513
3.712ProSer: 3.712 ± 0.546
3.299ProThr: 3.299 ± 0.49
4.537ProVal: 4.537 ± 0.461
1.083ProTrp: 1.083 ± 0.24
1.289ProTyr: 1.289 ± 0.244
0.0ProXaa: 0.0 ± 0.0
Gln
4.485GlnAla: 4.485 ± 0.486
0.361GlnCys: 0.361 ± 0.16
0.928GlnAsp: 0.928 ± 0.214
1.495GlnGlu: 1.495 ± 0.251
1.083GlnPhe: 1.083 ± 0.261
2.887GlnGly: 2.887 ± 0.323
0.825GlnHis: 0.825 ± 0.217
2.268GlnIle: 2.268 ± 0.319
0.825GlnLys: 0.825 ± 0.199
2.423GlnLeu: 2.423 ± 0.336
1.289GlnMet: 1.289 ± 0.244
0.773GlnAsn: 0.773 ± 0.169
1.753GlnPro: 1.753 ± 0.333
1.237GlnGln: 1.237 ± 0.268
3.402GlnArg: 3.402 ± 0.442
1.237GlnSer: 1.237 ± 0.244
1.804GlnThr: 1.804 ± 0.282
3.093GlnVal: 3.093 ± 0.429
0.876GlnTrp: 0.876 ± 0.252
1.237GlnTyr: 1.237 ± 0.217
0.0GlnXaa: 0.0 ± 0.0
Arg
7.269ArgAla: 7.269 ± 0.63
1.134ArgCys: 1.134 ± 0.274
4.33ArgAsp: 4.33 ± 0.495
5.001ArgGlu: 5.001 ± 0.552
2.268ArgPhe: 2.268 ± 0.341
5.001ArgGly: 5.001 ± 0.567
2.268ArgHis: 2.268 ± 0.401
2.938ArgIle: 2.938 ± 0.42
2.887ArgLys: 2.887 ± 0.403
6.032ArgLeu: 6.032 ± 0.567
1.907ArgMet: 1.907 ± 0.277
2.32ArgAsn: 2.32 ± 0.405
3.557ArgPro: 3.557 ± 0.467
3.145ArgGln: 3.145 ± 0.48
6.856ArgArg: 6.856 ± 0.778
2.784ArgSer: 2.784 ± 0.427
4.279ArgThr: 4.279 ± 0.493
5.98ArgVal: 5.98 ± 0.637
1.495ArgTrp: 1.495 ± 0.252
2.371ArgTyr: 2.371 ± 0.389
0.0ArgXaa: 0.0 ± 0.0
Ser
7.011SerAla: 7.011 ± 0.859
0.516SerCys: 0.516 ± 0.177
2.887SerAsp: 2.887 ± 0.411
2.474SerGlu: 2.474 ± 0.359
1.237SerPhe: 1.237 ± 0.211
4.382SerGly: 4.382 ± 0.565
1.134SerHis: 1.134 ± 0.268
1.753SerIle: 1.753 ± 0.343
0.979SerLys: 0.979 ± 0.264
4.073SerLeu: 4.073 ± 0.481
1.392SerMet: 1.392 ± 0.267
1.34SerAsn: 1.34 ± 0.292
3.145SerPro: 3.145 ± 0.43
1.598SerGln: 1.598 ± 0.262
3.042SerArg: 3.042 ± 0.412
2.629SerSer: 2.629 ± 0.427
3.248SerThr: 3.248 ± 0.358
3.557SerVal: 3.557 ± 0.491
1.289SerTrp: 1.289 ± 0.315
1.237SerTyr: 1.237 ± 0.24
0.0SerXaa: 0.0 ± 0.0
Thr
6.65ThrAla: 6.65 ± 0.72
0.567ThrCys: 0.567 ± 0.186
3.763ThrAsp: 3.763 ± 0.44
4.021ThrGlu: 4.021 ± 0.501
1.959ThrPhe: 1.959 ± 0.291
5.31ThrGly: 5.31 ± 0.671
1.34ThrHis: 1.34 ± 0.274
3.093ThrIle: 3.093 ± 0.457
1.804ThrLys: 1.804 ± 0.315
4.382ThrLeu: 4.382 ± 0.443
0.722ThrMet: 0.722 ± 0.189
1.443ThrAsn: 1.443 ± 0.295
4.382ThrPro: 4.382 ± 0.448
1.65ThrGln: 1.65 ± 0.294
4.124ThrArg: 4.124 ± 0.461
3.093ThrSer: 3.093 ± 0.524
3.506ThrThr: 3.506 ± 0.439
5.877ThrVal: 5.877 ± 0.537
1.237ThrTrp: 1.237 ± 0.222
1.289ThrTyr: 1.289 ± 0.264
0.0ThrXaa: 0.0 ± 0.0
Val
9.949ValAla: 9.949 ± 0.832
1.598ValCys: 1.598 ± 0.324
6.65ValAsp: 6.65 ± 0.704
4.794ValGlu: 4.794 ± 0.564
1.547ValPhe: 1.547 ± 0.28
6.702ValGly: 6.702 ± 0.809
1.907ValHis: 1.907 ± 0.396
3.145ValIle: 3.145 ± 0.391
2.681ValLys: 2.681 ± 0.316
6.547ValLeu: 6.547 ± 0.624
1.856ValMet: 1.856 ± 0.295
2.371ValAsn: 2.371 ± 0.273
4.897ValPro: 4.897 ± 0.496
2.578ValGln: 2.578 ± 0.44
5.413ValArg: 5.413 ± 0.542
3.969ValSer: 3.969 ± 0.422
5.155ValThr: 5.155 ± 0.488
6.856ValVal: 6.856 ± 0.721
1.959ValTrp: 1.959 ± 0.326
1.34ValTyr: 1.34 ± 0.289
0.0ValXaa: 0.0 ± 0.0
Trp
2.011TrpAla: 2.011 ± 0.329
0.309TrpCys: 0.309 ± 0.109
0.825TrpAsp: 0.825 ± 0.225
0.825TrpGlu: 0.825 ± 0.241
0.412TrpPhe: 0.412 ± 0.161
1.443TrpGly: 1.443 ± 0.27
0.412TrpHis: 0.412 ± 0.15
1.083TrpIle: 1.083 ± 0.229
0.361TrpLys: 0.361 ± 0.127
2.629TrpLeu: 2.629 ± 0.387
0.206TrpMet: 0.206 ± 0.115
0.722TrpAsn: 0.722 ± 0.18
0.876TrpPro: 0.876 ± 0.201
1.083TrpGln: 1.083 ± 0.25
2.887TrpArg: 2.887 ± 0.398
0.825TrpSer: 0.825 ± 0.267
1.237TrpThr: 1.237 ± 0.248
1.701TrpVal: 1.701 ± 0.258
0.464TrpTrp: 0.464 ± 0.146
0.619TrpTyr: 0.619 ± 0.161
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.474TyrAla: 2.474 ± 0.283
0.412TyrCys: 0.412 ± 0.144
1.495TyrAsp: 1.495 ± 0.26
1.856TyrGlu: 1.856 ± 0.323
0.619TyrPhe: 0.619 ± 0.155
2.062TyrGly: 2.062 ± 0.365
0.361TyrHis: 0.361 ± 0.136
0.619TyrIle: 0.619 ± 0.212
0.619TyrLys: 0.619 ± 0.224
1.959TyrLeu: 1.959 ± 0.344
0.567TyrMet: 0.567 ± 0.17
0.67TyrAsn: 0.67 ± 0.153
0.928TyrPro: 0.928 ± 0.235
1.134TyrGln: 1.134 ± 0.331
1.701TyrArg: 1.701 ± 0.3
1.443TyrSer: 1.443 ± 0.264
1.34TyrThr: 1.34 ± 0.299
2.526TyrVal: 2.526 ± 0.312
0.412TyrTrp: 0.412 ± 0.198
0.567TyrTyr: 0.567 ± 0.131
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 103 proteins (19399 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski