Streptococcus phage phiARI0460-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A141E092|A0A141E092_9CAUD CBM_5_12 domain protein OS=Streptococcus phage phiARI0460-1 OX=1701824 GN=phiARI0460-1_16 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.42FKK4 pKa = 10.53IKK6 pKa = 10.21QDD8 pKa = 3.67FYY10 pKa = 11.5DD11 pKa = 3.28WEE13 pKa = 5.08SNVKK17 pKa = 9.99RR18 pKa = 11.84LAGEE22 pKa = 3.73EE23 pKa = 4.17LEE25 pKa = 4.4ITEE28 pKa = 4.03EE29 pKa = 4.46RR30 pKa = 11.84YY31 pKa = 10.36AEE33 pKa = 4.17LADD36 pKa = 4.47NIASNGVAISDD47 pKa = 3.89VLEE50 pKa = 4.98KK51 pKa = 10.45ILPEE55 pKa = 4.04PEE57 pKa = 4.1FLEE60 pKa = 4.42EE61 pKa = 4.41DD62 pKa = 3.37

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A141E0B7|A0A141E0B7_9CAUD Uncharacterized protein OS=Streptococcus phage phiARI0460-1 OX=1701824 GN=phiARI0460-1_42 PE=4 SV=1
MM1 pKa = 8.02HH2 pKa = 7.61EE3 pKa = 4.27ICPGRR8 pKa = 11.84LTPEE12 pKa = 3.75VTRR15 pKa = 11.84LLNEE19 pKa = 4.22KK20 pKa = 10.47FGTTYY25 pKa = 10.53TKK27 pKa = 9.64TQIGEE32 pKa = 3.8VRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84LGLPVGKK43 pKa = 10.29VYY45 pKa = 10.35QGKK48 pKa = 9.17LLTKK52 pKa = 9.08EE53 pKa = 3.64QHH55 pKa = 6.9DD56 pKa = 4.5YY57 pKa = 11.1LVSIQKK63 pKa = 10.33NKK65 pKa = 9.84ISRR68 pKa = 11.84DD69 pKa = 3.29VANEE73 pKa = 3.68MNLKK77 pKa = 10.42FGLSLTEE84 pKa = 4.06KK85 pKa = 10.22QIKK88 pKa = 9.48SYY90 pKa = 10.67RR91 pKa = 11.84RR92 pKa = 11.84NNNLHH97 pKa = 6.24SGLTGRR103 pKa = 11.84FEE105 pKa = 5.1KK106 pKa = 11.07GQTPHH111 pKa = 6.58NKK113 pKa = 8.94GKK115 pKa = 10.51KK116 pKa = 8.0YY117 pKa = 10.9PNMPKK122 pKa = 10.37NGGQFKK128 pKa = 10.52KK129 pKa = 10.86GNRR132 pKa = 11.84PPNYY136 pKa = 9.98VPVGTINYY144 pKa = 7.06TTDD147 pKa = 3.55GYY149 pKa = 9.73PKK151 pKa = 10.44EE152 pKa = 4.95KK153 pKa = 9.75IGEE156 pKa = 4.09PNQWVLKK163 pKa = 9.51HH164 pKa = 5.61RR165 pKa = 11.84KK166 pKa = 8.01VWEE169 pKa = 4.09EE170 pKa = 3.39HH171 pKa = 6.51HH172 pKa = 6.78GPIPKK177 pKa = 9.74GHH179 pKa = 6.74SIVFLDD185 pKa = 4.36GDD187 pKa = 3.73KK188 pKa = 11.07TNYY191 pKa = 10.39DD192 pKa = 3.87ILNLACLSKK201 pKa = 11.55NEE203 pKa = 4.07IARR206 pKa = 11.84MNQNHH211 pKa = 7.16LFTSNADD218 pKa = 3.59LTKK221 pKa = 10.82SGIGLTKK228 pKa = 9.48LTNKK232 pKa = 9.28IRR234 pKa = 11.84EE235 pKa = 4.16VEE237 pKa = 4.19KK238 pKa = 11.28NGG240 pKa = 3.57

Molecular weight: 27.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12135

43

1217

237.9

26.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.716 ± 0.504

0.503 ± 0.098

6.222 ± 0.271

7.845 ± 0.429

4.096 ± 0.207

6.403 ± 0.488

1.376 ± 0.15

6.51 ± 0.26

9.015 ± 0.384

8.133 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.332 ± 0.178

5.529 ± 0.268

2.81 ± 0.221

3.98 ± 0.189

4.31 ± 0.281

6.189 ± 0.255

6.197 ± 0.34

6.494 ± 0.318

1.426 ± 0.13

3.898 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski