Rhodococcus sp. RD6.2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5489 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2YMA5|A0A0K2YMA5_9NOCA Uncharacterized protein OS=Rhodococcus sp. RD6.2 OX=260936 GN=RHCRD62_40556 PE=4 SV=1
MM1 pKa = 7.38SHH3 pKa = 7.26PFDD6 pKa = 4.14MNSDD10 pKa = 3.62PTVVIPRR17 pKa = 11.84VEE19 pKa = 4.13PEE21 pKa = 4.4PEE23 pKa = 4.28PEE25 pKa = 4.21PEE27 pKa = 4.19PTPEE31 pKa = 4.13APASSGARR39 pKa = 11.84GKK41 pKa = 9.96IVAVGASVAALVLGAAAGYY60 pKa = 8.81FAGSSGSSSEE70 pKa = 4.02PATVPVAATTVAPPTTTSAAPAPGPGMFLAASGTVDD106 pKa = 4.3TIAGDD111 pKa = 3.86TFVLKK116 pKa = 10.97AEE118 pKa = 5.21DD119 pKa = 3.88GTKK122 pKa = 9.08VTIGTTAATRR132 pKa = 11.84IVTLQGDD139 pKa = 3.93EE140 pKa = 4.69FEE142 pKa = 5.03DD143 pKa = 5.13LEE145 pKa = 5.58IGDD148 pKa = 4.05AAVVQGEE155 pKa = 4.03QSGGNFVADD164 pKa = 3.83LVIAGALSGLLPTTEE179 pKa = 3.96AAAPANAVQQQTMDD193 pKa = 3.64QVPQPTWRR201 pKa = 11.84ATAAPTTTQVPEE213 pKa = 4.18SAPMDD218 pKa = 3.7GSGPSGGYY226 pKa = 10.46GPTGQYY232 pKa = 10.95GPDD235 pKa = 3.62GDD237 pKa = 4.34YY238 pKa = 11.52GPDD241 pKa = 3.54GDD243 pKa = 4.8YY244 pKa = 11.32GPSGNYY250 pKa = 10.08GPDD253 pKa = 3.37GDD255 pKa = 4.67YY256 pKa = 11.31GPSGNYY262 pKa = 9.4GPRR265 pKa = 11.84GDD267 pKa = 3.64YY268 pKa = 10.61GPRR271 pKa = 11.84GGYY274 pKa = 10.21GPDD277 pKa = 3.23GGYY280 pKa = 10.96GPDD283 pKa = 3.3GGYY286 pKa = 10.78GPRR289 pKa = 3.92

Molecular weight: 28.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2YSY6|A0A0K2YSY6_9NOCA Uncharacterized protein OS=Rhodococcus sp. RD6.2 OX=260936 GN=RHCRD62_70299 PE=4 SV=1
MM1 pKa = 7.15HH2 pKa = 7.4RR3 pKa = 11.84SSRR6 pKa = 11.84RR7 pKa = 11.84AAAFRR12 pKa = 11.84PRR14 pKa = 11.84RR15 pKa = 11.84NAAMYY20 pKa = 8.46PRR22 pKa = 11.84RR23 pKa = 11.84TAVTSPRR30 pKa = 11.84RR31 pKa = 11.84NAAMFPRR38 pKa = 11.84RR39 pKa = 11.84AIRR42 pKa = 11.84RR43 pKa = 11.84PRR45 pKa = 11.84PRR47 pKa = 11.84IGSSLDD53 pKa = 3.2HH54 pKa = 6.75RR55 pKa = 11.84PARR58 pKa = 11.84APRR61 pKa = 3.74

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5489

0

5489

1736644

20

14536

316.4

33.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.105 ± 0.04

0.76 ± 0.012

6.505 ± 0.035

5.322 ± 0.027

2.917 ± 0.018

9.19 ± 0.035

2.154 ± 0.015

3.889 ± 0.025

1.885 ± 0.023

9.832 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.952 ± 0.013

1.99 ± 0.018

5.683 ± 0.027

2.664 ± 0.018

7.498 ± 0.032

5.551 ± 0.024

6.426 ± 0.028

9.274 ± 0.035

1.447 ± 0.014

1.954 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski