Pseudomonas phage D3112 (Bacteriophage D3112)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Casadabanvirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q6TM72|ACR30_BPD31 Anti-CRISPR protein 30 OS=Pseudomonas phage D3112 OX=10708 GN=orf30 PE=1 SV=1
MM1 pKa = 6.84STQYY5 pKa = 10.32TYY7 pKa = 11.15EE8 pKa = 3.92QIAEE12 pKa = 4.19DD13 pKa = 3.73FRR15 pKa = 11.84LWGEE19 pKa = 4.04YY20 pKa = 9.14MDD22 pKa = 5.63PNAEE26 pKa = 3.96MTEE29 pKa = 4.4EE30 pKa = 4.03EE31 pKa = 4.3FQALSTEE38 pKa = 4.53EE39 pKa = 4.0KK40 pKa = 10.51VAMQVEE46 pKa = 4.56AFGAEE51 pKa = 3.91AA52 pKa = 4.36

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6TM82|Q6TM82_BPD31 Uncharacterized protein orf20 OS=Pseudomonas phage D3112 OX=10708 GN=orf20 PE=4 SV=1
MM1 pKa = 7.71ALQPRR6 pKa = 11.84GIRR9 pKa = 11.84NNNPGNIVWSARR21 pKa = 11.84NNWQGQLPHH30 pKa = 6.52NPKK33 pKa = 9.83IEE35 pKa = 3.9PRR37 pKa = 11.84FARR40 pKa = 11.84FDD42 pKa = 3.52TAHH45 pKa = 6.2NGIRR49 pKa = 11.84ALAKK53 pKa = 10.57LLLNYY58 pKa = 10.23RR59 pKa = 11.84KK60 pKa = 9.76VDD62 pKa = 3.32GLRR65 pKa = 11.84TVEE68 pKa = 3.98SLIARR73 pKa = 11.84WAPSNEE79 pKa = 3.72NDD81 pKa = 2.89TRR83 pKa = 11.84AYY85 pKa = 8.05ATAVARR91 pKa = 11.84AMGVPPQAGLHH102 pKa = 5.82MDD104 pKa = 3.53QDD106 pKa = 4.01TLAALVTAIIRR117 pKa = 11.84HH118 pKa = 5.57EE119 pKa = 4.47NGQQPYY125 pKa = 9.68SAEE128 pKa = 3.99QIAQAVRR135 pKa = 11.84EE136 pKa = 4.2VLL138 pKa = 3.49

Molecular weight: 15.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11811

34

735

214.7

23.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.598 ± 0.501

0.855 ± 0.094

5.884 ± 0.236

5.91 ± 0.336

2.769 ± 0.189

7.882 ± 0.387

1.592 ± 0.146

4.25 ± 0.199

3.471 ± 0.217

9.322 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.201 ± 0.143

2.921 ± 0.243

5.046 ± 0.222

5.173 ± 0.3

7.374 ± 0.386

5.867 ± 0.263

5.622 ± 0.279

6.689 ± 0.272

1.82 ± 0.104

2.752 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski