Amino acid dipepetide frequency for Ciona savignyi (Pacific transparent sea squirt)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.762AlaAla: 4.762 ± 0.031	1.351AlaCys: 1.351 ± 0.018	3.147AlaAsp: 3.147 ± 0.023	3.58AlaGlu: 3.58 ± 0.027	2.514AlaPhe: 2.514 ± 0.021	3.978AlaGly: 3.978 ± 0.028	1.355AlaHis: 1.355 ± 0.014	3.658AlaIle: 3.658 ± 0.023	3.564AlaLys: 3.564 ± 0.024	5.697AlaLeu: 5.697 ± 0.034	1.668AlaMet: 1.668 ± 0.014	2.632AlaAsn: 2.632 ± 0.02	2.632AlaPro: 2.632 ± 0.03	2.326AlaGln: 2.326 ± 0.021	2.85AlaArg: 2.85 ± 0.017	4.677AlaSer: 4.677 ± 0.027	3.871AlaThr: 3.871 ± 0.025	4.544AlaVal: 4.544 ± 0.028	0.768AlaTrp: 0.768 ± 0.01	1.774AlaTyr: 1.774 ± 0.014	0.0AlaXaa: 0.0 ± 0.0
Cys	1.381CysAla: 1.381 ± 0.024	0.638CysCys: 0.638 ± 0.013	1.439CysAsp: 1.439 ± 0.025	1.48CysGlu: 1.48 ± 0.029	0.998CysPhe: 0.998 ± 0.012	1.588CysGly: 1.588 ± 0.027	0.674CysHis: 0.674 ± 0.015	1.396CysIle: 1.396 ± 0.018	1.301CysLys: 1.301 ± 0.018	2.02CysLeu: 2.02 ± 0.02	0.56CysMet: 0.56 ± 0.01	1.331CysAsn: 1.331 ± 0.026	1.108CysPro: 1.108 ± 0.02	0.935CysGln: 0.935 ± 0.018	1.154CysArg: 1.154 ± 0.016	2.07CysSer: 2.07 ± 0.035	1.566CysThr: 1.566 ± 0.038	1.636CysVal: 1.636 ± 0.024	0.262CysTrp: 0.262 ± 0.006	0.703CysTyr: 0.703 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	3.274AspAla: 3.274 ± 0.022	1.158AspCys: 1.158 ± 0.021	3.543AspAsp: 3.543 ± 0.027	3.905AspGlu: 3.905 ± 0.032	2.361AspPhe: 2.361 ± 0.021	3.895AspGly: 3.895 ± 0.037	1.24AspHis: 1.24 ± 0.012	3.339AspIle: 3.339 ± 0.021	2.893AspLys: 2.893 ± 0.021	4.825AspLeu: 4.825 ± 0.029	1.283AspMet: 1.283 ± 0.012	2.369AspAsn: 2.369 ± 0.022	2.528AspPro: 2.528 ± 0.023	1.928AspGln: 1.928 ± 0.014	2.508AspArg: 2.508 ± 0.02	3.682AspSer: 3.682 ± 0.023	2.649AspThr: 2.649 ± 0.02	4.001AspVal: 4.001 ± 0.026	0.71AspTrp: 0.71 ± 0.011	1.813AspTyr: 1.813 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	3.814GluAla: 3.814 ± 0.024	1.42GluCys: 1.42 ± 0.033	3.553GluAsp: 3.553 ± 0.029	5.022GluGlu: 5.022 ± 0.041	2.257GluPhe: 2.257 ± 0.015	3.015GluGly: 3.015 ± 0.021	1.316GluHis: 1.316 ± 0.011	3.62GluIle: 3.62 ± 0.023	4.681GluLys: 4.681 ± 0.047	5.402GluLeu: 5.402 ± 0.033	1.687GluMet: 1.687 ± 0.015	3.278GluAsn: 3.278 ± 0.025	2.223GluPro: 2.223 ± 0.023	2.483GluGln: 2.483 ± 0.023	3.244GluArg: 3.244 ± 0.022	3.994GluSer: 3.994 ± 0.025	3.25GluThr: 3.25 ± 0.022	3.935GluVal: 3.935 ± 0.026	0.734GluTrp: 0.734 ± 0.01	1.833GluTyr: 1.833 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	2.519PheAla: 2.519 ± 0.021	0.966PheCys: 0.966 ± 0.013	2.383PheAsp: 2.383 ± 0.02	2.242PheGlu: 2.242 ± 0.018	1.968PhePhe: 1.968 ± 0.022	2.707PheGly: 2.707 ± 0.022	1.158PheHis: 1.158 ± 0.014	2.613PheIle: 2.613 ± 0.02	2.394PheLys: 2.394 ± 0.024	3.983PheLeu: 3.983 ± 0.029	1.016PheMet: 1.016 ± 0.01	2.171PheAsn: 2.171 ± 0.018	1.925PhePro: 1.925 ± 0.017	1.702PheGln: 1.702 ± 0.017	2.053PheArg: 2.053 ± 0.024	3.284PheSer: 3.284 ± 0.021	2.672PheThr: 2.672 ± 0.025	2.888PheVal: 2.888 ± 0.019	0.517PheTrp: 0.517 ± 0.007	1.555PheTyr: 1.555 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	3.657GlyAla: 3.657 ± 0.026	1.263GlyCys: 1.263 ± 0.015	3.236GlyAsp: 3.236 ± 0.029	3.621GlyGlu: 3.621 ± 0.04	2.855GlyPhe: 2.855 ± 0.027	4.482GlyGly: 4.482 ± 0.039	1.463GlyHis: 1.463 ± 0.015	3.474GlyIle: 3.474 ± 0.025	3.895GlyLys: 3.895 ± 0.024	5.174GlyLeu: 5.174 ± 0.04	1.585GlyMet: 1.585 ± 0.015	2.949GlyAsn: 2.949 ± 0.024	2.492GlyPro: 2.492 ± 0.059	2.208GlyGln: 2.208 ± 0.024	3.118GlyArg: 3.118 ± 0.025	4.917GlySer: 4.917 ± 0.034	3.459GlyThr: 3.459 ± 0.037	4.055GlyVal: 4.055 ± 0.031	0.833GlyTrp: 0.833 ± 0.011	2.406GlyTyr: 2.406 ± 0.029	0.0GlyXaa: 0.0 ± 0.0
His	1.373HisAla: 1.373 ± 0.014	0.616HisCys: 0.616 ± 0.008	1.234HisAsp: 1.234 ± 0.013	1.397HisGlu: 1.397 ± 0.014	1.109HisPhe: 1.109 ± 0.012	1.526HisGly: 1.526 ± 0.016	0.843HisHis: 0.843 ± 0.014	1.399HisIle: 1.399 ± 0.015	1.408HisLys: 1.408 ± 0.016	2.447HisLeu: 2.447 ± 0.029	0.606HisMet: 0.606 ± 0.009	1.235HisAsn: 1.235 ± 0.016	1.396HisPro: 1.396 ± 0.017	1.106HisGln: 1.106 ± 0.018	1.477HisArg: 1.477 ± 0.019	1.816HisSer: 1.816 ± 0.014	1.564HisThr: 1.564 ± 0.025	1.598HisVal: 1.598 ± 0.016	0.344HisTrp: 0.344 ± 0.006	0.912HisTyr: 0.912 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	3.623IleAla: 3.623 ± 0.022	1.338IleCys: 1.338 ± 0.013	3.11IleAsp: 3.11 ± 0.023	3.236IleGlu: 3.236 ± 0.023	2.606IlePhe: 2.606 ± 0.023	3.41IleGly: 3.41 ± 0.029	1.603IleHis: 1.603 ± 0.024	3.336IleIle: 3.336 ± 0.026	3.38IleLys: 3.38 ± 0.022	5.235IleLeu: 5.235 ± 0.031	1.382IleMet: 1.382 ± 0.014	2.858IleAsn: 2.858 ± 0.024	2.852IlePro: 2.852 ± 0.019	2.298IleGln: 2.298 ± 0.018	2.847IleArg: 2.847 ± 0.018	4.523IleSer: 4.523 ± 0.027	3.565IleThr: 3.565 ± 0.029	3.618IleVal: 3.618 ± 0.025	0.639IleTrp: 0.639 ± 0.009	1.87IleTyr: 1.87 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	3.457LysAla: 3.457 ± 0.023	1.412LysCys: 1.412 ± 0.022	3.105LysAsp: 3.105 ± 0.024	4.12LysGlu: 4.12 ± 0.024	2.444LysPhe: 2.444 ± 0.02	2.98LysGly: 2.98 ± 0.031	1.607LysHis: 1.607 ± 0.023	3.48LysIle: 3.48 ± 0.023	4.583LysLys: 4.583 ± 0.04	5.896LysLeu: 5.896 ± 0.031	1.619LysMet: 1.619 ± 0.016	2.803LysAsn: 2.803 ± 0.019	2.877LysPro: 2.877 ± 0.033	2.712LysGln: 2.712 ± 0.021	3.491LysArg: 3.491 ± 0.029	4.714LysSer: 4.714 ± 0.036	3.339LysThr: 3.339 ± 0.023	3.988LysVal: 3.988 ± 0.024	0.771LysTrp: 0.771 ± 0.01	2.08LysTyr: 2.08 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	5.667LeuAla: 5.667 ± 0.03	2.084LeuCys: 2.084 ± 0.022	4.775LeuAsp: 4.775 ± 0.029	5.436LeuGlu: 5.436 ± 0.035	3.903LeuPhe: 3.903 ± 0.03	4.851LeuGly: 4.851 ± 0.029	2.463LeuHis: 2.463 ± 0.02	4.86LeuIle: 4.86 ± 0.031	5.89LeuLys: 5.89 ± 0.036	8.85LeuLeu: 8.85 ± 0.056	2.19LeuMet: 2.19 ± 0.017	4.362LeuAsn: 4.362 ± 0.024	4.479LeuPro: 4.479 ± 0.033	4.184LeuGln: 4.184 ± 0.026	4.869LeuArg: 4.869 ± 0.031	6.98LeuSer: 6.98 ± 0.032	5.231LeuThr: 5.231 ± 0.025	5.79LeuVal: 5.79 ± 0.031	1.133LeuTrp: 1.133 ± 0.024	2.798LeuTyr: 2.798 ± 0.019	0.001LeuXaa: 0.001 ± 0.0
Met	1.719MetAla: 1.719 ± 0.014	0.539MetCys: 0.539 ± 0.01	1.397MetAsp: 1.397 ± 0.013	1.712MetGlu: 1.712 ± 0.014	1.126MetPhe: 1.126 ± 0.012	1.421MetGly: 1.421 ± 0.014	0.622MetHis: 0.622 ± 0.009	1.325MetIle: 1.325 ± 0.014	1.699MetLys: 1.699 ± 0.013	2.388MetLeu: 2.388 ± 0.019	0.769MetMet: 0.769 ± 0.01	1.191MetAsn: 1.191 ± 0.013	0.99MetPro: 0.99 ± 0.01	1.095MetGln: 1.095 ± 0.013	1.227MetArg: 1.227 ± 0.013	1.875MetSer: 1.875 ± 0.013	1.433MetThr: 1.433 ± 0.015	1.653MetVal: 1.653 ± 0.013	0.315MetTrp: 0.315 ± 0.005	0.775MetTyr: 0.775 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.791AsnAla: 2.791 ± 0.021	1.375AsnCys: 1.375 ± 0.034	2.489AsnAsp: 2.489 ± 0.018	2.994AsnGlu: 2.994 ± 0.024	2.064AsnPhe: 2.064 ± 0.016	3.298AsnGly: 3.298 ± 0.037	1.159AsnHis: 1.159 ± 0.015	3.097AsnIle: 3.097 ± 0.022	2.852AsnLys: 2.852 ± 0.018	4.451AsnLeu: 4.451 ± 0.027	1.191AsnMet: 1.191 ± 0.011	2.497AsnAsn: 2.497 ± 0.02	2.651AsnPro: 2.651 ± 0.023	2.084AsnGln: 2.084 ± 0.018	2.219AsnArg: 2.219 ± 0.015	3.534AsnSer: 3.534 ± 0.023	2.692AsnThr: 2.692 ± 0.024	3.178AsnVal: 3.178 ± 0.024	0.563AsnTrp: 0.563 ± 0.008	1.604AsnTyr: 1.604 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	2.789ProAla: 2.789 ± 0.019	1.02ProCys: 1.02 ± 0.026	2.522ProAsp: 2.522 ± 0.016	2.865ProGlu: 2.865 ± 0.025	1.695ProPhe: 1.695 ± 0.015	3.496ProGly: 3.496 ± 0.087	1.327ProHis: 1.327 ± 0.024	2.419ProIle: 2.419 ± 0.019	2.497ProLys: 2.497 ± 0.02	3.698ProLeu: 3.698 ± 0.02	1.063ProMet: 1.063 ± 0.013	2.323ProAsn: 2.323 ± 0.019	3.476ProPro: 3.476 ± 0.044	1.914ProGln: 1.914 ± 0.018	2.205ProArg: 2.205 ± 0.018	3.927ProSer: 3.927 ± 0.03	3.128ProThr: 3.128 ± 0.03	3.08ProVal: 3.08 ± 0.023	0.512ProTrp: 0.512 ± 0.007	1.566ProTyr: 1.566 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	2.559GlnAla: 2.559 ± 0.02	1.076GlnCys: 1.076 ± 0.019	1.884GlnAsp: 1.884 ± 0.015	2.472GlnGlu: 2.472 ± 0.022	1.649GlnPhe: 1.649 ± 0.015	2.022GlnGly: 2.022 ± 0.025	1.135GlnHis: 1.135 ± 0.013	2.295GlnIle: 2.295 ± 0.018	2.278GlnLys: 2.278 ± 0.019	4.027GlnLeu: 4.027 ± 0.027	1.069GlnMet: 1.069 ± 0.014	1.965GlnAsn: 1.965 ± 0.016	1.956GlnPro: 1.956 ± 0.021	2.244GlnGln: 2.244 ± 0.024	2.329GlnArg: 2.329 ± 0.02	2.901GlnSer: 2.901 ± 0.021	2.208GlnThr: 2.208 ± 0.024	2.618GlnVal: 2.618 ± 0.016	0.527GlnTrp: 0.527 ± 0.008	1.255GlnTyr: 1.255 ± 0.012	0.0GlnXaa: 0.0 ± 0.0
Arg	2.836ArgAla: 2.836 ± 0.018	1.226ArgCys: 1.226 ± 0.021	2.674ArgAsp: 2.674 ± 0.019	2.947ArgGlu: 2.947 ± 0.022	2.149ArgPhe: 2.149 ± 0.018	2.855ArgGly: 2.855 ± 0.031	1.484ArgHis: 1.484 ± 0.013	2.977ArgIle: 2.977 ± 0.023	3.668ArgLys: 3.668 ± 0.027	4.555ArgLeu: 4.555 ± 0.025	1.338ArgMet: 1.338 ± 0.014	2.564ArgAsn: 2.564 ± 0.016	2.247ArgPro: 2.247 ± 0.026	1.996ArgGln: 1.996 ± 0.016	3.38ArgArg: 3.38 ± 0.029	3.83ArgSer: 3.83 ± 0.027	2.743ArgThr: 2.743 ± 0.021	3.05ArgVal: 3.05 ± 0.027	0.721ArgTrp: 0.721 ± 0.009	1.701ArgTyr: 1.701 ± 0.015	0.0ArgXaa: 0.0 ± 0.0
Ser	4.582SerAla: 4.582 ± 0.024	1.951SerCys: 1.951 ± 0.031	4.252SerAsp: 4.252 ± 0.028	4.113SerGlu: 4.113 ± 0.027	3.444SerPhe: 3.444 ± 0.031	5.064SerGly: 5.064 ± 0.031	1.863SerHis: 1.863 ± 0.02	4.319SerIle: 4.319 ± 0.027	4.393SerLys: 4.393 ± 0.027	6.728SerLeu: 6.728 ± 0.038	1.902SerMet: 1.902 ± 0.016	3.9SerAsn: 3.9 ± 0.029	3.862SerPro: 3.862 ± 0.035	2.768SerGln: 2.768 ± 0.017	3.767SerArg: 3.767 ± 0.025	7.141SerSer: 7.141 ± 0.046	4.748SerThr: 4.748 ± 0.027	4.996SerVal: 4.996 ± 0.029	0.943SerTrp: 0.943 ± 0.011	2.51SerTyr: 2.51 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	3.661ThrAla: 3.661 ± 0.024	1.937ThrCys: 1.937 ± 0.057	3.034ThrAsp: 3.034 ± 0.025	3.259ThrGlu: 3.259 ± 0.021	2.407ThrPhe: 2.407 ± 0.023	3.976ThrGly: 3.976 ± 0.033	1.415ThrHis: 1.415 ± 0.017	3.272ThrIle: 3.272 ± 0.023	3.279ThrLys: 3.279 ± 0.023	5.063ThrLeu: 5.063 ± 0.028	1.402ThrMet: 1.402 ± 0.014	2.875ThrAsn: 2.875 ± 0.024	3.136ThrPro: 3.136 ± 0.029	2.112ThrGln: 2.112 ± 0.016	2.637ThrArg: 2.637 ± 0.024	5.057ThrSer: 5.057 ± 0.03	3.979ThrThr: 3.979 ± 0.05	3.869ThrVal: 3.869 ± 0.03	0.788ThrTrp: 0.788 ± 0.013	1.853ThrTyr: 1.853 ± 0.016	0.0ThrXaa: 0.0 ± 0.0
Val	4.45ValAla: 4.45 ± 0.026	1.797ValCys: 1.797 ± 0.025	3.666ValAsp: 3.666 ± 0.023	3.914ValGlu: 3.914 ± 0.028	2.949ValPhe: 2.949 ± 0.022	3.953ValGly: 3.953 ± 0.03	1.553ValHis: 1.553 ± 0.014	3.837ValIle: 3.837 ± 0.026	3.894ValLys: 3.894 ± 0.026	6.101ValLeu: 6.101 ± 0.032	1.71ValMet: 1.71 ± 0.014	3.089ValAsn: 3.089 ± 0.023	2.89ValPro: 2.89 ± 0.025	2.529ValGln: 2.529 ± 0.017	3.155ValArg: 3.155 ± 0.019	4.763ValSer: 4.763 ± 0.027	4.238ValThr: 4.238 ± 0.038	4.974ValVal: 4.974 ± 0.033	0.831ValTrp: 0.831 ± 0.011	2.034ValTyr: 2.034 ± 0.015	0.0ValXaa: 0.0 ± 0.0
Trp	0.623TrpAla: 0.623 ± 0.008	0.302TrpCys: 0.302 ± 0.007	0.67TrpAsp: 0.67 ± 0.011	0.69TrpGlu: 0.69 ± 0.01	0.561TrpPhe: 0.561 ± 0.007	0.695TrpGly: 0.695 ± 0.009	0.257TrpHis: 0.257 ± 0.005	0.697TrpIle: 0.697 ± 0.01	0.886TrpLys: 0.886 ± 0.01	1.206TrpLeu: 1.206 ± 0.012	0.361TrpMet: 0.361 ± 0.006	0.702TrpAsn: 0.702 ± 0.008	0.424TrpPro: 0.424 ± 0.007	0.488TrpGln: 0.488 ± 0.008	0.695TrpArg: 0.695 ± 0.009	1.077TrpSer: 1.077 ± 0.018	0.743TrpThr: 0.743 ± 0.012	0.79TrpVal: 0.79 ± 0.01	0.24TrpTrp: 0.24 ± 0.004	0.476TrpTyr: 0.476 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.814TyrAla: 1.814 ± 0.016	0.772TyrCys: 0.772 ± 0.012	1.789TyrAsp: 1.789 ± 0.015	1.755TyrGlu: 1.755 ± 0.015	1.657TyrPhe: 1.657 ± 0.018	1.932TyrGly: 1.932 ± 0.015	0.872TyrHis: 0.872 ± 0.012	1.916TyrIle: 1.916 ± 0.014	2.047TyrLys: 2.047 ± 0.03	3.046TyrLeu: 3.046 ± 0.02	0.872TyrMet: 0.872 ± 0.009	1.691TyrAsn: 1.691 ± 0.018	1.377TyrPro: 1.377 ± 0.013	1.356TyrGln: 1.356 ± 0.015	1.714TyrArg: 1.714 ± 0.014	2.481TyrSer: 2.481 ± 0.023	1.932TyrThr: 1.932 ± 0.019	2.066TyrVal: 2.066 ± 0.016	0.433TyrTrp: 0.433 ± 0.009	1.2TyrTyr: 1.2 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.006XaaXaa: 0.006 ± 0.001

Statistics based on 20004 proteins (9644551 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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