Virgibacillus massiliensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Virgibacillus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A024QGD6|A0A024QGD6_9BACI Uncharacterized protein OS=Virgibacillus massiliensis OX=1462526 GN=BN990_03987 PE=4 SV=1
MM1 pKa = 7.8LAQTNHH7 pKa = 6.88KK8 pKa = 9.16ARR10 pKa = 11.84KK11 pKa = 8.37YY12 pKa = 8.82VLSTAFVTSLALTPIFSGSVFANAGAGASEE42 pKa = 4.33GDD44 pKa = 3.69LTISEE49 pKa = 4.55SNIPNVEE56 pKa = 4.0QQEE59 pKa = 4.43DD60 pKa = 4.0TSSNEE65 pKa = 3.09ASLIQRR71 pKa = 11.84GDD73 pKa = 3.24VSEE76 pKa = 4.36SVEE79 pKa = 4.16DD80 pKa = 3.94VQALLQDD87 pKa = 3.36QGYY90 pKa = 7.75YY91 pKa = 10.03TYY93 pKa = 10.35TVDD96 pKa = 5.68GIFGPITEE104 pKa = 4.27QAVRR108 pKa = 11.84DD109 pKa = 3.92YY110 pKa = 11.21QADD113 pKa = 2.97QGLQVDD119 pKa = 5.6GIVGPNTSEE128 pKa = 3.98AMGLSGSEE136 pKa = 4.1SSNDD140 pKa = 3.32LTITEE145 pKa = 4.79SGDD148 pKa = 3.43NSDD151 pKa = 3.32IQADD155 pKa = 3.32IVAAAEE161 pKa = 4.32SVIGTPYY168 pKa = 10.07VWGGTTTDD176 pKa = 3.88GMDD179 pKa = 2.84SSGFINYY186 pKa = 8.91VFDD189 pKa = 3.56QVGIDD194 pKa = 3.34VSRR197 pKa = 11.84THH199 pKa = 8.37SEE201 pKa = 3.18MWQNDD206 pKa = 4.08GVHH209 pKa = 6.22VDD211 pKa = 3.65APSVGDD217 pKa = 3.45VVFFEE222 pKa = 4.58GTYY225 pKa = 7.29EE226 pKa = 4.1TEE228 pKa = 4.4GASHH232 pKa = 6.3SGIYY236 pKa = 10.04IGDD239 pKa = 3.51NQMIHH244 pKa = 6.87AGNDD248 pKa = 3.66GVSVADD254 pKa = 4.07ISIDD258 pKa = 3.29YY259 pKa = 8.08WKK261 pKa = 10.54NHH263 pKa = 6.04YY264 pKa = 9.97IGAKK268 pKa = 9.94SITEE272 pKa = 3.89

Molecular weight: 28.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A024Q6D6|A0A024Q6D6_9BACI Ribosomal-protein-alanine N-acetyltransferase OS=Virgibacillus massiliensis OX=1462526 GN=BN990_00355 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.35QPNNRR10 pKa = 11.84KK11 pKa = 9.22RR12 pKa = 11.84KK13 pKa = 8.02KK14 pKa = 8.47VHH16 pKa = 5.57GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 10.29NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4389

0

4389

1246616

29

2377

284.0

31.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.959 ± 0.035

0.674 ± 0.011

5.289 ± 0.035

7.207 ± 0.046

4.496 ± 0.033

6.753 ± 0.038

2.2 ± 0.022

8.289 ± 0.044

6.505 ± 0.041

9.416 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.848 ± 0.018

4.776 ± 0.034

3.578 ± 0.025

4.116 ± 0.032

3.832 ± 0.029

6.0 ± 0.031

5.637 ± 0.024

6.669 ± 0.032

1.073 ± 0.016

3.682 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski