Bifiguratus adelaidae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina; Endogonomycetes; Endogonales; Endogonales incertae sedis; Bifiguratus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5718 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A261XVQ4|A0A261XVQ4_9FUNG V-type proton ATPase subunit a OS=Bifiguratus adelaidae OX=1938954 GN=BZG36_04462 PE=3 SV=1
MM1 pKa = 7.52NFDD4 pKa = 3.5QQQQQGSLGQRR15 pKa = 11.84QSGLNRR21 pKa = 11.84QEE23 pKa = 3.99EE24 pKa = 5.74GYY26 pKa = 10.07GQSDD30 pKa = 3.99YY31 pKa = 11.29GQQGDD36 pKa = 3.78ISQQNMGSDD45 pKa = 3.41SGQFGGMQPSYY56 pKa = 10.98GGNTQQPYY64 pKa = 10.33SSSDD68 pKa = 3.33YY69 pKa = 10.62SQQDD73 pKa = 3.19PSQQLGSQGQQQGQKK88 pKa = 9.11SQGGEE93 pKa = 3.96SQMQAPSAGSDD104 pKa = 2.94NTQFIDD110 pKa = 4.08ASQRR114 pKa = 11.84DD115 pKa = 3.82QQQGSNQNWW124 pKa = 3.1

Molecular weight: 13.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A261XZT6|A0A261XZT6_9FUNG Uncharacterized protein OS=Bifiguratus adelaidae OX=1938954 GN=BZG36_03679 PE=4 SV=1
MM1 pKa = 6.95STPRR5 pKa = 11.84LTSFRR10 pKa = 11.84RR11 pKa = 11.84FAVISGHH18 pKa = 6.46PPFAAQSPLAAGQVRR33 pKa = 11.84LLHH36 pKa = 6.45RR37 pKa = 11.84SSNRR41 pKa = 11.84YY42 pKa = 7.98FKK44 pKa = 10.7CQSAVAQTPRR54 pKa = 11.84AEE56 pKa = 4.22PGIIATIQRR65 pKa = 11.84DD66 pKa = 3.9FKK68 pKa = 11.3SIFPRR73 pKa = 11.84RR74 pKa = 11.84SSPLGHH80 pKa = 6.16RR81 pKa = 11.84RR82 pKa = 11.84HH83 pKa = 6.17RR84 pKa = 11.84KK85 pKa = 8.91GRR87 pKa = 11.84ALWAAVLQCRR97 pKa = 11.84KK98 pKa = 8.86WLNDD102 pKa = 3.21PFSIQFRR109 pKa = 11.84TPRR112 pKa = 11.84LFQVVAATYY121 pKa = 9.32QQFLRR126 pKa = 11.84DD127 pKa = 3.3IYY129 pKa = 10.63HH130 pKa = 6.58IYY132 pKa = 10.43DD133 pKa = 3.69RR134 pKa = 11.84PSTVDD139 pKa = 2.92MNYY142 pKa = 10.98AMSAKK147 pKa = 10.23SLFWNSRR154 pKa = 11.84WNHH157 pKa = 4.96RR158 pKa = 11.84LKK160 pKa = 11.11VPLAGPILDD169 pKa = 3.75AMTPEE174 pKa = 4.52SLHH177 pKa = 5.85TMIALPFATPKK188 pKa = 10.6HH189 pKa = 5.68LVFEE193 pKa = 4.44YY194 pKa = 11.03GDD196 pKa = 3.74MRR198 pKa = 11.84IHH200 pKa = 7.88ASRR203 pKa = 11.84NSLLSIADD211 pKa = 4.22TITSKK216 pKa = 11.1GSTSAFILTNINILKK231 pKa = 10.19ADD233 pKa = 4.33LDD235 pKa = 4.97AICSSPSSQRR245 pKa = 11.84IKK247 pKa = 10.66RR248 pKa = 11.84LPNAGGSSHH257 pKa = 6.57KK258 pKa = 10.82SEE260 pKa = 3.92VLSYY264 pKa = 11.22VFLEE268 pKa = 4.12RR269 pKa = 11.84MLGCEE274 pKa = 3.98LLKK277 pKa = 10.36TEE279 pKa = 4.53MEE281 pKa = 4.57IIYY284 pKa = 9.26EE285 pKa = 3.98PRR287 pKa = 11.84GGPMVDD293 pKa = 3.64YY294 pKa = 10.67SIHH297 pKa = 6.53LPQHH301 pKa = 6.68LGSTWLAVSVTRR313 pKa = 11.84AIAFGRR319 pKa = 11.84NFTLQDD325 pKa = 3.14AEE327 pKa = 4.02KK328 pKa = 10.44LIRR331 pKa = 11.84HH332 pKa = 6.19KK333 pKa = 11.02LLGVFFSNQTIVQRR347 pKa = 11.84QRR349 pKa = 11.84PSRR352 pKa = 11.84QILHH356 pKa = 5.04IWARR360 pKa = 11.84EE361 pKa = 3.91GKK363 pKa = 8.97VARR366 pKa = 11.84ILSKK370 pKa = 10.13AWSRR374 pKa = 11.84VMDD377 pKa = 5.05DD378 pKa = 3.95MPKK381 pKa = 8.23STIVIVSVVNMDD393 pKa = 3.87AVFLNKK399 pKa = 10.0AFKK402 pKa = 10.2QQ403 pKa = 3.62

Molecular weight: 45.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5718

0

5718

3128832

51

5291

547.2

61.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.006 ± 0.022

1.222 ± 0.01

5.9 ± 0.018

6.19 ± 0.028

3.788 ± 0.017

6.064 ± 0.028

2.575 ± 0.013

5.301 ± 0.019

5.263 ± 0.022

9.135 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.012

4.039 ± 0.018

5.437 ± 0.027

4.369 ± 0.017

5.638 ± 0.023

7.947 ± 0.029

6.069 ± 0.016

6.289 ± 0.016

1.272 ± 0.01

3.138 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski