Massilia sp. Root351

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia; unclassified Massilia

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6059 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6VK14|A0A0Q6VK14_9BURK Uncharacterized protein OS=Massilia sp. Root351 OX=1736522 GN=ASD15_21885 PE=4 SV=1
MM1 pKa = 7.73GYY3 pKa = 9.55ILHH6 pKa = 6.01GTDD9 pKa = 2.98WDD11 pKa = 4.34DD12 pKa = 5.66NIDD15 pKa = 3.78GTAGDD20 pKa = 4.57DD21 pKa = 4.36DD22 pKa = 5.5LYY24 pKa = 11.45GYY26 pKa = 10.73AGYY29 pKa = 10.82DD30 pKa = 3.2SLFGGRR36 pKa = 11.84GDD38 pKa = 4.87DD39 pKa = 3.93YY40 pKa = 11.77LNGGAGLDD48 pKa = 3.26EE49 pKa = 4.67AFYY52 pKa = 10.78NGYY55 pKa = 9.68RR56 pKa = 11.84GEE58 pKa = 4.19YY59 pKa = 9.97SVLADD64 pKa = 4.99GGQFWVNDD72 pKa = 3.61EE73 pKa = 4.44VGDD76 pKa = 3.98EE77 pKa = 4.25GLDD80 pKa = 3.24LLMNVEE86 pKa = 4.45RR87 pKa = 11.84LNFKK91 pKa = 10.61DD92 pKa = 3.53GAVALDD98 pKa = 3.21IDD100 pKa = 4.59GNGGEE105 pKa = 4.89AYY107 pKa = 10.31RR108 pKa = 11.84LYY110 pKa = 10.25QAAFNRR116 pKa = 11.84EE117 pKa = 3.83PDD119 pKa = 3.57EE120 pKa = 4.49VGLGFWMAHH129 pKa = 5.12MDD131 pKa = 3.09QGYY134 pKa = 8.72SLRR137 pKa = 11.84AIAQNFVTSNEE148 pKa = 3.68FRR150 pKa = 11.84DD151 pKa = 4.15TYY153 pKa = 10.85GANASNAEE161 pKa = 3.89IVLRR165 pKa = 11.84FYY167 pKa = 11.38FNVLHH172 pKa = 7.05RR173 pKa = 11.84APDD176 pKa = 3.58QEE178 pKa = 4.74GLNFWVTALNTGQATVADD196 pKa = 4.24VLTGFSEE203 pKa = 4.36SAEE206 pKa = 4.09NYY208 pKa = 8.99AQLIGKK214 pKa = 7.65MQDD217 pKa = 3.52GIDD220 pKa = 3.75YY221 pKa = 10.31QPWGG225 pKa = 3.73

Molecular weight: 24.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6VWP8|A0A0Q6VWP8_9BURK Uncharacterized protein OS=Massilia sp. Root351 OX=1736522 GN=ASD15_12740 PE=4 SV=1
MM1 pKa = 7.91PKK3 pKa = 9.63MKK5 pKa = 9.83TKK7 pKa = 10.72SSAKK11 pKa = 9.15KK12 pKa = 9.67RR13 pKa = 11.84FRR15 pKa = 11.84VRR17 pKa = 11.84PGGTVKK23 pKa = 10.54SGMAFKK29 pKa = 10.69RR30 pKa = 11.84HH31 pKa = 5.84ILTKK35 pKa = 9.78KK36 pKa = 3.98TTKK39 pKa = 10.23NKK41 pKa = 9.21RR42 pKa = 11.84QLRR45 pKa = 11.84GTRR48 pKa = 11.84NINASDD54 pKa = 3.65VTSVMRR60 pKa = 11.84MMPTAA65 pKa = 4.09

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6059

0

6059

2134414

47

3292

352.3

38.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.5 ± 0.046

0.872 ± 0.01

5.193 ± 0.02

5.079 ± 0.026

3.452 ± 0.018

8.464 ± 0.032

2.146 ± 0.015

4.358 ± 0.023

3.557 ± 0.029

10.577 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.015

2.974 ± 0.022

5.048 ± 0.024

4.172 ± 0.021

6.432 ± 0.031

5.563 ± 0.026

4.978 ± 0.025

7.131 ± 0.024

1.352 ± 0.013

2.603 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski