Amino acid dipepetide frequency for Streptococcus phage IPP30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.057AlaAla: 4.057 ± 1.285
0.518AlaCys: 0.518 ± 0.225
4.489AlaAsp: 4.489 ± 0.751
5.698AlaGlu: 5.698 ± 0.722
3.108AlaPhe: 3.108 ± 0.408
3.971AlaGly: 3.971 ± 0.77
0.518AlaHis: 0.518 ± 0.179
5.611AlaIle: 5.611 ± 1.185
5.093AlaLys: 5.093 ± 0.669
7.683AlaLeu: 7.683 ± 0.944
1.985AlaMet: 1.985 ± 0.366
4.23AlaAsn: 4.23 ± 0.783
1.64AlaPro: 1.64 ± 0.343
3.28AlaGln: 3.28 ± 0.775
2.935AlaArg: 2.935 ± 0.479
3.885AlaSer: 3.885 ± 0.791
4.489AlaThr: 4.489 ± 0.594
4.23AlaVal: 4.23 ± 0.721
0.863AlaTrp: 0.863 ± 0.287
1.985AlaTyr: 1.985 ± 0.467
0.0AlaXaa: 0.0 ± 0.0
Cys
0.259CysAla: 0.259 ± 0.126
0.0CysCys: 0.0 ± 0.0
0.173CysAsp: 0.173 ± 0.127
0.863CysGlu: 0.863 ± 0.385
0.259CysPhe: 0.259 ± 0.133
0.518CysGly: 0.518 ± 0.243
0.173CysHis: 0.173 ± 0.149
0.518CysIle: 0.518 ± 0.236
0.432CysLys: 0.432 ± 0.205
0.604CysLeu: 0.604 ± 0.26
0.259CysMet: 0.259 ± 0.229
0.086CysAsn: 0.086 ± 0.099
0.086CysPro: 0.086 ± 0.083
0.259CysGln: 0.259 ± 0.159
0.432CysArg: 0.432 ± 0.205
0.259CysSer: 0.259 ± 0.171
0.345CysThr: 0.345 ± 0.15
0.086CysVal: 0.086 ± 0.082
0.173CysTrp: 0.173 ± 0.097
0.604CysTyr: 0.604 ± 0.233
0.0CysXaa: 0.0 ± 0.0
Asp
3.367AspAla: 3.367 ± 0.446
0.345AspCys: 0.345 ± 0.21
3.28AspAsp: 3.28 ± 0.608
3.712AspGlu: 3.712 ± 0.697
2.244AspPhe: 2.244 ± 0.442
4.748AspGly: 4.748 ± 0.73
0.777AspHis: 0.777 ± 0.267
5.093AspIle: 5.093 ± 0.61
5.266AspLys: 5.266 ± 0.901
5.352AspLeu: 5.352 ± 0.69
1.122AspMet: 1.122 ± 0.356
2.849AspAsn: 2.849 ± 0.683
1.554AspPro: 1.554 ± 0.349
1.468AspGln: 1.468 ± 0.284
1.899AspArg: 1.899 ± 0.479
3.021AspSer: 3.021 ± 0.578
2.762AspThr: 2.762 ± 0.428
3.626AspVal: 3.626 ± 0.695
0.863AspTrp: 0.863 ± 0.231
3.28AspTyr: 3.28 ± 0.619
0.0AspXaa: 0.0 ± 0.0
Glu
6.043GluAla: 6.043 ± 1.241
0.345GluCys: 0.345 ± 0.155
3.712GluAsp: 3.712 ± 0.532
6.561GluGlu: 6.561 ± 1.015
3.798GluPhe: 3.798 ± 0.552
3.194GluGly: 3.194 ± 0.46
0.95GluHis: 0.95 ± 0.333
6.647GluIle: 6.647 ± 0.79
7.079GluLys: 7.079 ± 0.968
9.755GluLeu: 9.755 ± 0.889
2.244GluMet: 2.244 ± 0.663
5.266GluAsn: 5.266 ± 0.558
1.381GluPro: 1.381 ± 0.475
3.453GluGln: 3.453 ± 0.723
3.108GluArg: 3.108 ± 0.565
3.626GluSer: 3.626 ± 0.519
3.885GluThr: 3.885 ± 0.699
5.007GluVal: 5.007 ± 0.769
1.295GluTrp: 1.295 ± 0.372
2.503GluTyr: 2.503 ± 0.612
0.0GluXaa: 0.0 ± 0.0
Phe
2.676PheAla: 2.676 ± 0.557
0.173PheCys: 0.173 ± 0.135
3.712PheAsp: 3.712 ± 0.622
2.762PheGlu: 2.762 ± 0.465
1.899PhePhe: 1.899 ± 0.682
2.849PheGly: 2.849 ± 0.486
0.691PheHis: 0.691 ± 0.338
3.367PheIle: 3.367 ± 0.712
4.144PheLys: 4.144 ± 0.533
2.676PheLeu: 2.676 ± 0.57
1.295PheMet: 1.295 ± 0.4
2.762PheAsn: 2.762 ± 0.377
1.381PhePro: 1.381 ± 0.379
1.381PheGln: 1.381 ± 0.352
2.158PheArg: 2.158 ± 0.402
2.503PheSer: 2.503 ± 0.538
2.59PheThr: 2.59 ± 0.472
2.158PheVal: 2.158 ± 0.511
0.604PheTrp: 0.604 ± 0.204
1.813PheTyr: 1.813 ± 0.344
0.0PheXaa: 0.0 ± 0.0
Gly
3.194GlyAla: 3.194 ± 0.606
0.345GlyCys: 0.345 ± 0.139
3.539GlyAsp: 3.539 ± 0.595
3.626GlyGlu: 3.626 ± 0.47
3.021GlyPhe: 3.021 ± 0.731
4.316GlyGly: 4.316 ± 0.675
1.036GlyHis: 1.036 ± 0.343
4.403GlyIle: 4.403 ± 0.674
4.316GlyLys: 4.316 ± 0.583
5.266GlyLeu: 5.266 ± 0.669
1.64GlyMet: 1.64 ± 0.349
3.712GlyAsn: 3.712 ± 0.615
0.863GlyPro: 0.863 ± 0.226
2.59GlyGln: 2.59 ± 0.58
3.194GlyArg: 3.194 ± 0.501
3.626GlySer: 3.626 ± 0.601
3.367GlyThr: 3.367 ± 0.538
3.971GlyVal: 3.971 ± 0.526
1.468GlyTrp: 1.468 ± 0.499
2.331GlyTyr: 2.331 ± 0.366
0.0GlyXaa: 0.0 ± 0.0
His
0.432HisAla: 0.432 ± 0.157
0.259HisCys: 0.259 ± 0.163
0.777HisAsp: 0.777 ± 0.294
1.295HisGlu: 1.295 ± 0.34
1.209HisPhe: 1.209 ± 0.271
0.863HisGly: 0.863 ± 0.26
0.259HisHis: 0.259 ± 0.176
1.899HisIle: 1.899 ± 0.37
0.863HisLys: 0.863 ± 0.238
1.468HisLeu: 1.468 ± 0.413
0.345HisMet: 0.345 ± 0.155
0.95HisAsn: 0.95 ± 0.289
0.432HisPro: 0.432 ± 0.168
0.518HisGln: 0.518 ± 0.265
0.259HisArg: 0.259 ± 0.16
1.295HisSer: 1.295 ± 0.438
0.777HisThr: 0.777 ± 0.288
0.863HisVal: 0.863 ± 0.249
0.086HisTrp: 0.086 ± 0.085
0.604HisTyr: 0.604 ± 0.207
0.0HisXaa: 0.0 ± 0.0
Ile
6.733IleAla: 6.733 ± 0.795
0.691IleCys: 0.691 ± 0.248
3.971IleAsp: 3.971 ± 0.577
5.352IleGlu: 5.352 ± 0.644
2.417IlePhe: 2.417 ± 0.462
3.971IleGly: 3.971 ± 0.676
0.95IleHis: 0.95 ± 0.262
4.748IleIle: 4.748 ± 0.887
5.87IleLys: 5.87 ± 0.699
5.439IleLeu: 5.439 ± 0.928
1.209IleMet: 1.209 ± 0.275
3.798IleAsn: 3.798 ± 0.495
1.899IlePro: 1.899 ± 0.317
2.762IleGln: 2.762 ± 0.412
3.539IleArg: 3.539 ± 0.617
6.474IleSer: 6.474 ± 1.243
4.403IleThr: 4.403 ± 0.702
4.057IleVal: 4.057 ± 0.71
0.691IleTrp: 0.691 ± 0.231
1.554IleTyr: 1.554 ± 0.411
0.0IleXaa: 0.0 ± 0.0
Lys
6.215LysAla: 6.215 ± 0.741
0.432LysCys: 0.432 ± 0.203
5.18LysAsp: 5.18 ± 0.755
7.079LysGlu: 7.079 ± 0.96
2.849LysPhe: 2.849 ± 0.545
4.403LysGly: 4.403 ± 0.77
2.158LysHis: 2.158 ± 0.478
5.093LysIle: 5.093 ± 0.679
5.266LysLys: 5.266 ± 0.884
5.611LysLeu: 5.611 ± 0.615
1.899LysMet: 1.899 ± 0.394
5.18LysAsn: 5.18 ± 0.632
2.417LysPro: 2.417 ± 0.552
4.662LysGln: 4.662 ± 0.592
4.23LysArg: 4.23 ± 0.793
4.662LysSer: 4.662 ± 0.635
5.007LysThr: 5.007 ± 0.629
5.18LysVal: 5.18 ± 0.804
1.036LysTrp: 1.036 ± 0.316
2.935LysTyr: 2.935 ± 0.527
0.0LysXaa: 0.0 ± 0.0
Leu
6.561LeuAla: 6.561 ± 0.84
0.518LeuCys: 0.518 ± 0.29
6.043LeuAsp: 6.043 ± 0.552
7.251LeuGlu: 7.251 ± 0.693
4.23LeuPhe: 4.23 ± 0.606
5.525LeuGly: 5.525 ± 0.558
1.209LeuHis: 1.209 ± 0.285
4.489LeuIle: 4.489 ± 0.548
7.338LeuLys: 7.338 ± 0.793
5.956LeuLeu: 5.956 ± 1.07
2.072LeuMet: 2.072 ± 0.534
4.834LeuAsn: 4.834 ± 0.674
2.331LeuPro: 2.331 ± 0.551
2.676LeuGln: 2.676 ± 0.883
4.748LeuArg: 4.748 ± 0.583
6.388LeuSer: 6.388 ± 0.832
5.266LeuThr: 5.266 ± 0.57
4.144LeuVal: 4.144 ± 0.595
0.777LeuTrp: 0.777 ± 0.244
2.762LeuTyr: 2.762 ± 0.481
0.0LeuXaa: 0.0 ± 0.0
Met
1.727MetAla: 1.727 ± 0.434
0.086MetCys: 0.086 ± 0.085
1.381MetAsp: 1.381 ± 0.439
2.072MetGlu: 2.072 ± 0.468
0.691MetPhe: 0.691 ± 0.271
1.122MetGly: 1.122 ± 0.259
0.0MetHis: 0.0 ± 0.0
1.295MetIle: 1.295 ± 0.308
2.417MetLys: 2.417 ± 0.611
1.899MetLeu: 1.899 ± 0.468
0.691MetMet: 0.691 ± 0.267
2.072MetAsn: 2.072 ± 0.482
0.432MetPro: 0.432 ± 0.165
0.777MetGln: 0.777 ± 0.253
1.122MetArg: 1.122 ± 0.437
1.381MetSer: 1.381 ± 0.316
1.985MetThr: 1.985 ± 0.502
1.64MetVal: 1.64 ± 0.343
0.173MetTrp: 0.173 ± 0.142
0.604MetTyr: 0.604 ± 0.224
0.0MetXaa: 0.0 ± 0.0
Asn
3.971AsnAla: 3.971 ± 0.788
0.173AsnCys: 0.173 ± 0.108
2.331AsnAsp: 2.331 ± 0.48
4.575AsnGlu: 4.575 ± 0.619
2.676AsnPhe: 2.676 ± 0.424
4.662AsnGly: 4.662 ± 0.587
1.554AsnHis: 1.554 ± 0.405
4.057AsnIle: 4.057 ± 0.595
5.352AsnLys: 5.352 ± 0.59
4.575AsnLeu: 4.575 ± 0.675
0.863AsnMet: 0.863 ± 0.37
1.985AsnAsn: 1.985 ± 0.374
2.59AsnPro: 2.59 ± 0.504
3.798AsnGln: 3.798 ± 0.618
2.503AsnArg: 2.503 ± 0.468
3.539AsnSer: 3.539 ± 0.704
2.676AsnThr: 2.676 ± 0.393
4.316AsnVal: 4.316 ± 0.65
0.95AsnTrp: 0.95 ± 0.311
1.295AsnTyr: 1.295 ± 0.442
0.0AsnXaa: 0.0 ± 0.0
Pro
2.072ProAla: 2.072 ± 0.392
0.345ProCys: 0.345 ± 0.191
2.244ProAsp: 2.244 ± 0.528
2.072ProGlu: 2.072 ± 0.41
1.122ProPhe: 1.122 ± 0.445
0.604ProGly: 0.604 ± 0.246
0.518ProHis: 0.518 ± 0.255
1.813ProIle: 1.813 ± 0.516
2.503ProLys: 2.503 ± 0.595
2.158ProLeu: 2.158 ± 0.532
0.173ProMet: 0.173 ± 0.119
0.95ProAsn: 0.95 ± 0.281
0.432ProPro: 0.432 ± 0.21
1.468ProGln: 1.468 ± 0.413
0.777ProArg: 0.777 ± 0.299
1.468ProSer: 1.468 ± 0.329
2.158ProThr: 2.158 ± 0.597
2.072ProVal: 2.072 ± 0.326
0.432ProTrp: 0.432 ± 0.156
0.863ProTyr: 0.863 ± 0.299
0.0ProXaa: 0.0 ± 0.0
Gln
4.921GlnAla: 4.921 ± 1.002
0.086GlnCys: 0.086 ± 0.093
1.381GlnAsp: 1.381 ± 0.346
4.144GlnGlu: 4.144 ± 0.774
1.899GlnPhe: 1.899 ± 0.372
2.158GlnGly: 2.158 ± 0.366
0.604GlnHis: 0.604 ± 0.21
2.503GlnIle: 2.503 ± 0.466
3.453GlnLys: 3.453 ± 0.492
2.59GlnLeu: 2.59 ± 0.487
1.295GlnMet: 1.295 ± 0.372
2.417GlnAsn: 2.417 ± 0.388
1.295GlnPro: 1.295 ± 0.34
1.381GlnGln: 1.381 ± 0.38
2.244GlnArg: 2.244 ± 0.466
3.626GlnSer: 3.626 ± 0.421
2.762GlnThr: 2.762 ± 0.571
2.762GlnVal: 2.762 ± 0.532
0.345GlnTrp: 0.345 ± 0.191
1.209GlnTyr: 1.209 ± 0.37
0.0GlnXaa: 0.0 ± 0.0
Arg
3.453ArgAla: 3.453 ± 0.649
0.173ArgCys: 0.173 ± 0.133
1.985ArgAsp: 1.985 ± 0.387
3.712ArgGlu: 3.712 ± 0.732
1.727ArgPhe: 1.727 ± 0.477
1.899ArgGly: 1.899 ± 0.485
0.604ArgHis: 0.604 ± 0.359
3.712ArgIle: 3.712 ± 0.616
3.885ArgLys: 3.885 ± 0.713
4.489ArgLeu: 4.489 ± 0.687
1.209ArgMet: 1.209 ± 0.251
3.798ArgAsn: 3.798 ± 0.517
1.554ArgPro: 1.554 ± 0.38
1.985ArgGln: 1.985 ± 0.632
2.59ArgArg: 2.59 ± 0.656
1.727ArgSer: 1.727 ± 0.343
2.417ArgThr: 2.417 ± 0.93
2.417ArgVal: 2.417 ± 0.482
0.777ArgTrp: 0.777 ± 0.254
2.244ArgTyr: 2.244 ± 0.575
0.0ArgXaa: 0.0 ± 0.0
Ser
4.748SerAla: 4.748 ± 0.882
0.432SerCys: 0.432 ± 0.161
3.194SerAsp: 3.194 ± 0.547
4.921SerGlu: 4.921 ± 0.888
2.935SerPhe: 2.935 ± 0.451
4.403SerGly: 4.403 ± 0.891
0.691SerHis: 0.691 ± 0.241
3.539SerIle: 3.539 ± 0.478
4.921SerLys: 4.921 ± 0.636
4.316SerLeu: 4.316 ± 0.496
1.468SerMet: 1.468 ± 0.508
3.626SerAsn: 3.626 ± 0.6
1.727SerPro: 1.727 ± 0.344
2.331SerGln: 2.331 ± 0.709
2.935SerArg: 2.935 ± 0.819
3.712SerSer: 3.712 ± 0.617
3.194SerThr: 3.194 ± 0.691
4.662SerVal: 4.662 ± 1.104
0.863SerTrp: 0.863 ± 0.213
2.417SerTyr: 2.417 ± 0.446
0.0SerXaa: 0.0 ± 0.0
Thr
4.144ThrAla: 4.144 ± 1.045
0.259ThrCys: 0.259 ± 0.184
4.489ThrAsp: 4.489 ± 0.75
3.885ThrGlu: 3.885 ± 0.664
2.935ThrPhe: 2.935 ± 0.743
3.367ThrGly: 3.367 ± 0.617
0.777ThrHis: 0.777 ± 0.245
5.007ThrIle: 5.007 ± 0.664
4.489ThrLys: 4.489 ± 0.57
5.439ThrLeu: 5.439 ± 0.64
0.95ThrMet: 0.95 ± 0.405
2.849ThrAsn: 2.849 ± 0.513
0.863ThrPro: 0.863 ± 0.293
2.676ThrGln: 2.676 ± 0.694
2.676ThrArg: 2.676 ± 0.52
3.798ThrSer: 3.798 ± 0.558
4.748ThrThr: 4.748 ± 0.797
4.489ThrVal: 4.489 ± 0.852
0.432ThrTrp: 0.432 ± 0.193
1.985ThrTyr: 1.985 ± 0.482
0.0ThrXaa: 0.0 ± 0.0
Val
3.108ValAla: 3.108 ± 0.574
0.345ValCys: 0.345 ± 0.18
3.108ValAsp: 3.108 ± 0.419
6.215ValGlu: 6.215 ± 0.784
2.158ValPhe: 2.158 ± 0.438
4.403ValGly: 4.403 ± 0.663
0.95ValHis: 0.95 ± 0.278
3.885ValIle: 3.885 ± 0.599
5.784ValLys: 5.784 ± 0.786
4.834ValLeu: 4.834 ± 0.662
1.381ValMet: 1.381 ± 0.336
3.885ValAsn: 3.885 ± 0.512
1.64ValPro: 1.64 ± 0.439
2.59ValGln: 2.59 ± 0.492
2.072ValArg: 2.072 ± 0.497
3.453ValSer: 3.453 ± 0.576
5.439ValThr: 5.439 ± 0.676
4.748ValVal: 4.748 ± 0.729
1.122ValTrp: 1.122 ± 0.345
1.64ValTyr: 1.64 ± 0.532
0.0ValXaa: 0.0 ± 0.0
Trp
0.777TrpAla: 0.777 ± 0.252
0.173TrpCys: 0.173 ± 0.13
0.432TrpAsp: 0.432 ± 0.181
1.209TrpGlu: 1.209 ± 0.398
0.518TrpPhe: 0.518 ± 0.226
0.691TrpGly: 0.691 ± 0.232
0.259TrpHis: 0.259 ± 0.181
1.036TrpIle: 1.036 ± 0.311
0.691TrpLys: 0.691 ± 0.257
1.036TrpLeu: 1.036 ± 0.201
0.432TrpMet: 0.432 ± 0.212
1.554TrpAsn: 1.554 ± 0.302
0.173TrpPro: 0.173 ± 0.13
1.036TrpGln: 1.036 ± 0.218
0.691TrpArg: 0.691 ± 0.27
0.95TrpSer: 0.95 ± 0.297
0.604TrpThr: 0.604 ± 0.193
0.432TrpVal: 0.432 ± 0.163
0.173TrpTrp: 0.173 ± 0.091
0.777TrpTyr: 0.777 ± 0.498
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.813TyrAla: 1.813 ± 0.31
0.691TyrCys: 0.691 ± 0.226
1.209TyrAsp: 1.209 ± 0.229
2.935TyrGlu: 2.935 ± 0.577
1.899TyrPhe: 1.899 ± 0.496
2.158TyrGly: 2.158 ± 0.458
0.777TyrHis: 0.777 ± 0.201
1.813TyrIle: 1.813 ± 0.346
2.331TyrLys: 2.331 ± 0.526
3.971TyrLeu: 3.971 ± 0.63
0.95TyrMet: 0.95 ± 0.323
1.64TyrAsn: 1.64 ± 0.474
1.554TyrPro: 1.554 ± 0.37
1.985TyrGln: 1.985 ± 0.445
2.244TyrArg: 2.244 ± 0.488
1.64TyrSer: 1.64 ± 0.339
1.381TyrThr: 1.381 ± 0.28
1.899TyrVal: 1.899 ± 0.356
0.518TyrTrp: 0.518 ± 0.191
2.072TyrTyr: 2.072 ± 0.711
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (11585 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski