Amino acid dipepetide frequency for Mycobacterium phage Duke13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.164AlaAla: 10.164 ± 0.734
1.174AlaCys: 1.174 ± 0.195
6.07AlaAsp: 6.07 ± 0.451
7.387AlaGlu: 7.387 ± 0.535
3.636AlaPhe: 3.636 ± 0.379
7.444AlaGly: 7.444 ± 0.706
2.29AlaHis: 2.29 ± 0.3
5.325AlaIle: 5.325 ± 0.432
3.98AlaLys: 3.98 ± 0.318
8.818AlaLeu: 8.818 ± 0.589
3.407AlaMet: 3.407 ± 0.353
3.149AlaAsn: 3.149 ± 0.355
4.237AlaPro: 4.237 ± 0.353
3.064AlaGln: 3.064 ± 0.325
6.299AlaArg: 6.299 ± 0.428
4.953AlaSer: 4.953 ± 0.429
5.268AlaThr: 5.268 ± 0.434
6.213AlaVal: 6.213 ± 0.41
1.947AlaTrp: 1.947 ± 0.281
2.548AlaTyr: 2.548 ± 0.243
0.0AlaXaa: 0.0 ± 0.0
Cys
0.888CysAla: 0.888 ± 0.187
0.115CysCys: 0.115 ± 0.057
1.288CysAsp: 1.288 ± 0.215
1.088CysGlu: 1.088 ± 0.198
0.344CysPhe: 0.344 ± 0.095
1.918CysGly: 1.918 ± 0.251
0.344CysHis: 0.344 ± 0.111
0.458CysIle: 0.458 ± 0.141
0.687CysLys: 0.687 ± 0.162
1.145CysLeu: 1.145 ± 0.215
0.286CysMet: 0.286 ± 0.081
0.63CysAsn: 0.63 ± 0.156
0.773CysPro: 0.773 ± 0.135
0.401CysGln: 0.401 ± 0.124
1.059CysArg: 1.059 ± 0.244
0.659CysSer: 0.659 ± 0.138
0.744CysThr: 0.744 ± 0.121
1.031CysVal: 1.031 ± 0.193
0.286CysTrp: 0.286 ± 0.085
0.458CysTyr: 0.458 ± 0.125
0.0CysXaa: 0.0 ± 0.0
Asp
6.013AspAla: 6.013 ± 0.458
1.031AspCys: 1.031 ± 0.181
3.98AspAsp: 3.98 ± 0.374
4.696AspGlu: 4.696 ± 0.523
2.09AspPhe: 2.09 ± 0.227
6.757AspGly: 6.757 ± 0.494
1.861AspHis: 1.861 ± 0.264
3.207AspIle: 3.207 ± 0.304
2.233AspLys: 2.233 ± 0.306
4.925AspLeu: 4.925 ± 0.302
0.916AspMet: 0.916 ± 0.158
2.29AspAsn: 2.29 ± 0.265
3.665AspPro: 3.665 ± 0.302
1.861AspGln: 1.861 ± 0.224
3.922AspArg: 3.922 ± 0.32
3.035AspSer: 3.035 ± 0.307
2.92AspThr: 2.92 ± 0.273
4.123AspVal: 4.123 ± 0.416
1.976AspTrp: 1.976 ± 0.246
2.491AspTyr: 2.491 ± 0.238
0.0AspXaa: 0.0 ± 0.0
Glu
6.757GluAla: 6.757 ± 0.507
1.088GluCys: 1.088 ± 0.199
3.922GluAsp: 3.922 ± 0.382
4.925GluGlu: 4.925 ± 0.477
2.863GluPhe: 2.863 ± 0.282
4.466GluGly: 4.466 ± 0.363
1.26GluHis: 1.26 ± 0.174
3.894GluIle: 3.894 ± 0.325
2.577GluLys: 2.577 ± 0.375
6.413GluLeu: 6.413 ± 0.496
2.29GluMet: 2.29 ± 0.277
1.89GluAsn: 1.89 ± 0.194
3.35GluPro: 3.35 ± 0.404
2.462GluGln: 2.462 ± 0.263
4.896GluArg: 4.896 ± 0.378
3.264GluSer: 3.264 ± 0.341
3.493GluThr: 3.493 ± 0.343
4.438GluVal: 4.438 ± 0.396
1.403GluTrp: 1.403 ± 0.216
2.663GluTyr: 2.663 ± 0.267
0.0GluXaa: 0.0 ± 0.0
Phe
2.691PheAla: 2.691 ± 0.26
0.515PheCys: 0.515 ± 0.132
2.405PheAsp: 2.405 ± 0.286
2.319PheGlu: 2.319 ± 0.249
1.174PhePhe: 1.174 ± 0.207
3.121PheGly: 3.121 ± 0.437
0.687PheHis: 0.687 ± 0.147
1.288PheIle: 1.288 ± 0.153
1.317PheLys: 1.317 ± 0.195
2.09PheLeu: 2.09 ± 0.232
0.83PheMet: 0.83 ± 0.148
1.632PheAsn: 1.632 ± 0.27
2.033PhePro: 2.033 ± 0.28
0.916PheGln: 0.916 ± 0.173
1.832PheArg: 1.832 ± 0.254
1.775PheSer: 1.775 ± 0.234
2.061PheThr: 2.061 ± 0.237
2.262PheVal: 2.262 ± 0.249
0.659PheTrp: 0.659 ± 0.156
0.773PheTyr: 0.773 ± 0.139
0.0PheXaa: 0.0 ± 0.0
Gly
7.129GlyAla: 7.129 ± 0.646
1.317GlyCys: 1.317 ± 0.207
5.469GlyAsp: 5.469 ± 0.455
5.612GlyGlu: 5.612 ± 0.439
3.293GlyPhe: 3.293 ± 0.384
9.076GlyGly: 9.076 ± 1.772
2.147GlyHis: 2.147 ± 0.257
4.037GlyIle: 4.037 ± 0.348
3.636GlyLys: 3.636 ± 0.354
7.301GlyLeu: 7.301 ± 0.585
2.29GlyMet: 2.29 ± 0.291
3.149GlyAsn: 3.149 ± 0.327
3.779GlyPro: 3.779 ± 0.352
2.577GlyGln: 2.577 ± 0.383
5.44GlyArg: 5.44 ± 0.342
5.068GlySer: 5.068 ± 0.429
5.583GlyThr: 5.583 ± 0.564
5.325GlyVal: 5.325 ± 0.319
2.205GlyTrp: 2.205 ± 0.264
2.949GlyTyr: 2.949 ± 0.261
0.0GlyXaa: 0.0 ± 0.0
His
1.546HisAla: 1.546 ± 0.223
0.515HisCys: 0.515 ± 0.13
1.661HisAsp: 1.661 ± 0.212
1.575HisGlu: 1.575 ± 0.224
0.515HisPhe: 0.515 ± 0.137
2.176HisGly: 2.176 ± 0.252
0.916HisHis: 0.916 ± 0.162
1.002HisIle: 1.002 ± 0.204
0.773HisLys: 0.773 ± 0.151
2.491HisLeu: 2.491 ± 0.287
0.601HisMet: 0.601 ± 0.129
0.573HisAsn: 0.573 ± 0.131
1.374HisPro: 1.374 ± 0.182
0.916HisGln: 0.916 ± 0.147
1.804HisArg: 1.804 ± 0.236
0.945HisSer: 0.945 ± 0.182
0.888HisThr: 0.888 ± 0.169
1.432HisVal: 1.432 ± 0.227
0.716HisTrp: 0.716 ± 0.146
0.744HisTyr: 0.744 ± 0.142
0.0HisXaa: 0.0 ± 0.0
Ile
5.583IleAla: 5.583 ± 0.435
0.515IleCys: 0.515 ± 0.113
3.751IleAsp: 3.751 ± 0.315
3.837IleGlu: 3.837 ± 0.31
1.002IlePhe: 1.002 ± 0.185
3.894IleGly: 3.894 ± 0.415
1.288IleHis: 1.288 ± 0.2
1.918IleIle: 1.918 ± 0.22
1.432IleLys: 1.432 ± 0.191
3.436IleLeu: 3.436 ± 0.336
0.888IleMet: 0.888 ± 0.165
1.718IleAsn: 1.718 ± 0.186
2.949IlePro: 2.949 ± 0.288
1.575IleGln: 1.575 ± 0.203
2.92IleArg: 2.92 ± 0.336
2.376IleSer: 2.376 ± 0.237
2.892IleThr: 2.892 ± 0.245
3.464IleVal: 3.464 ± 0.316
0.859IleTrp: 0.859 ± 0.165
1.203IleTyr: 1.203 ± 0.172
0.0IleXaa: 0.0 ± 0.0
Lys
4.094LysAla: 4.094 ± 0.417
0.687LysCys: 0.687 ± 0.138
2.061LysAsp: 2.061 ± 0.225
2.205LysGlu: 2.205 ± 0.299
1.145LysPhe: 1.145 ± 0.143
3.035LysGly: 3.035 ± 0.3
1.203LysHis: 1.203 ± 0.192
1.689LysIle: 1.689 ± 0.234
2.262LysLys: 2.262 ± 0.345
3.865LysLeu: 3.865 ± 0.358
1.489LysMet: 1.489 ± 0.202
0.802LysAsn: 0.802 ± 0.139
2.749LysPro: 2.749 ± 0.299
1.203LysGln: 1.203 ± 0.171
2.777LysArg: 2.777 ± 0.297
1.661LysSer: 1.661 ± 0.228
2.09LysThr: 2.09 ± 0.235
3.092LysVal: 3.092 ± 0.335
0.859LysTrp: 0.859 ± 0.152
1.346LysTyr: 1.346 ± 0.198
0.0LysXaa: 0.0 ± 0.0
Leu
9.276LeuAla: 9.276 ± 0.524
0.859LeuCys: 0.859 ± 0.167
5.469LeuAsp: 5.469 ± 0.356
5.01LeuGlu: 5.01 ± 0.395
2.605LeuPhe: 2.605 ± 0.245
6.786LeuGly: 6.786 ± 0.464
1.746LeuHis: 1.746 ± 0.224
3.035LeuIle: 3.035 ± 0.292
3.493LeuLys: 3.493 ± 0.338
5.841LeuLeu: 5.841 ± 0.446
1.746LeuMet: 1.746 ± 0.203
3.235LeuAsn: 3.235 ± 0.277
4.524LeuPro: 4.524 ± 0.335
2.806LeuGln: 2.806 ± 0.277
5.354LeuArg: 5.354 ± 0.413
4.982LeuSer: 4.982 ± 0.423
5.383LeuThr: 5.383 ± 0.338
5.268LeuVal: 5.268 ± 0.386
1.374LeuTrp: 1.374 ± 0.177
2.233LeuTyr: 2.233 ± 0.26
0.0LeuXaa: 0.0 ± 0.0
Met
2.319MetAla: 2.319 ± 0.233
0.229MetCys: 0.229 ± 0.092
1.288MetAsp: 1.288 ± 0.16
1.203MetGlu: 1.203 ± 0.188
0.601MetPhe: 0.601 ± 0.134
1.947MetGly: 1.947 ± 0.24
0.487MetHis: 0.487 ± 0.116
1.346MetIle: 1.346 ± 0.204
1.231MetLys: 1.231 ± 0.181
1.517MetLeu: 1.517 ± 0.214
0.573MetMet: 0.573 ± 0.107
1.174MetAsn: 1.174 ± 0.159
1.002MetPro: 1.002 ± 0.183
0.659MetGln: 0.659 ± 0.154
1.317MetArg: 1.317 ± 0.192
2.691MetSer: 2.691 ± 0.326
2.405MetThr: 2.405 ± 0.307
1.317MetVal: 1.317 ± 0.205
0.487MetTrp: 0.487 ± 0.12
0.429MetTyr: 0.429 ± 0.098
0.0MetXaa: 0.0 ± 0.0
Asn
3.264AsnAla: 3.264 ± 0.338
0.401AsnCys: 0.401 ± 0.142
2.033AsnAsp: 2.033 ± 0.203
1.832AsnGlu: 1.832 ± 0.266
1.145AsnPhe: 1.145 ± 0.178
3.98AsnGly: 3.98 ± 0.455
0.945AsnHis: 0.945 ± 0.145
1.661AsnIle: 1.661 ± 0.3
1.489AsnLys: 1.489 ± 0.232
2.577AsnLeu: 2.577 ± 0.281
0.429AsnMet: 0.429 ± 0.132
0.744AsnAsn: 0.744 ± 0.149
2.691AsnPro: 2.691 ± 0.262
0.83AsnGln: 0.83 ± 0.211
2.233AsnArg: 2.233 ± 0.281
1.947AsnSer: 1.947 ± 0.273
1.746AsnThr: 1.746 ± 0.214
2.119AsnVal: 2.119 ± 0.232
0.802AsnTrp: 0.802 ± 0.16
0.83AsnTyr: 0.83 ± 0.143
0.0AsnXaa: 0.0 ± 0.0
Pro
5.411ProAla: 5.411 ± 0.375
0.716ProCys: 0.716 ± 0.159
3.522ProAsp: 3.522 ± 0.383
4.781ProGlu: 4.781 ± 0.457
1.804ProPhe: 1.804 ± 0.229
5.411ProGly: 5.411 ± 0.628
1.002ProHis: 1.002 ± 0.149
2.405ProIle: 2.405 ± 0.249
2.147ProLys: 2.147 ± 0.235
4.066ProLeu: 4.066 ± 0.353
1.059ProMet: 1.059 ± 0.179
2.119ProAsn: 2.119 ± 0.252
2.949ProPro: 2.949 ± 0.297
1.46ProGln: 1.46 ± 0.195
3.006ProArg: 3.006 ± 0.3
2.663ProSer: 2.663 ± 0.371
2.949ProThr: 2.949 ± 0.378
4.466ProVal: 4.466 ± 0.323
1.288ProTrp: 1.288 ± 0.197
1.489ProTyr: 1.489 ± 0.197
0.0ProXaa: 0.0 ± 0.0
Gln
3.35GlnAla: 3.35 ± 0.337
0.515GlnCys: 0.515 ± 0.131
1.346GlnAsp: 1.346 ± 0.21
1.804GlnGlu: 1.804 ± 0.245
1.317GlnPhe: 1.317 ± 0.199
2.061GlnGly: 2.061 ± 0.262
0.659GlnHis: 0.659 ± 0.131
1.603GlnIle: 1.603 ± 0.177
1.718GlnLys: 1.718 ± 0.299
2.434GlnLeu: 2.434 ± 0.292
1.059GlnMet: 1.059 ± 0.153
0.888GlnAsn: 0.888 ± 0.185
2.176GlnPro: 2.176 ± 0.272
1.374GlnGln: 1.374 ± 0.256
2.233GlnArg: 2.233 ± 0.284
1.746GlnSer: 1.746 ± 0.227
1.718GlnThr: 1.718 ± 0.237
2.376GlnVal: 2.376 ± 0.294
0.659GlnTrp: 0.659 ± 0.135
0.687GlnTyr: 0.687 ± 0.119
0.0GlnXaa: 0.0 ± 0.0
Arg
6.499ArgAla: 6.499 ± 0.507
1.002ArgCys: 1.002 ± 0.183
4.18ArgAsp: 4.18 ± 0.369
4.237ArgGlu: 4.237 ± 0.426
2.061ArgPhe: 2.061 ± 0.246
5.182ArgGly: 5.182 ± 0.395
1.489ArgHis: 1.489 ± 0.227
3.378ArgIle: 3.378 ± 0.387
2.92ArgLys: 2.92 ± 0.351
4.61ArgLeu: 4.61 ± 0.401
1.746ArgMet: 1.746 ± 0.229
1.918ArgAsn: 1.918 ± 0.231
3.378ArgPro: 3.378 ± 0.415
2.29ArgGln: 2.29 ± 0.298
5.469ArgArg: 5.469 ± 0.537
3.264ArgSer: 3.264 ± 0.279
3.293ArgThr: 3.293 ± 0.372
4.896ArgVal: 4.896 ± 0.385
1.861ArgTrp: 1.861 ± 0.298
2.176ArgTyr: 2.176 ± 0.29
0.0ArgXaa: 0.0 ± 0.0
Ser
5.698SerAla: 5.698 ± 0.435
0.859SerCys: 0.859 ± 0.159
3.207SerAsp: 3.207 ± 0.347
3.522SerGlu: 3.522 ± 0.358
1.775SerPhe: 1.775 ± 0.29
5.096SerGly: 5.096 ± 0.54
1.117SerHis: 1.117 ± 0.175
3.064SerIle: 3.064 ± 0.316
2.033SerLys: 2.033 ± 0.223
4.696SerLeu: 4.696 ± 0.353
1.374SerMet: 1.374 ± 0.199
2.004SerAsn: 2.004 ± 0.297
2.834SerPro: 2.834 ± 0.276
1.632SerGln: 1.632 ± 0.223
3.321SerArg: 3.321 ± 0.314
3.293SerSer: 3.293 ± 0.402
2.777SerThr: 2.777 ± 0.224
3.493SerVal: 3.493 ± 0.398
1.489SerTrp: 1.489 ± 0.243
1.546SerTyr: 1.546 ± 0.221
0.0SerXaa: 0.0 ± 0.0
Thr
4.867ThrAla: 4.867 ± 0.401
1.088ThrCys: 1.088 ± 0.191
2.978ThrAsp: 2.978 ± 0.31
3.522ThrGlu: 3.522 ± 0.255
2.119ThrPhe: 2.119 ± 0.244
5.039ThrGly: 5.039 ± 0.426
1.145ThrHis: 1.145 ± 0.18
2.949ThrIle: 2.949 ± 0.281
1.775ThrLys: 1.775 ± 0.27
5.383ThrLeu: 5.383 ± 0.383
0.888ThrMet: 0.888 ± 0.171
1.661ThrAsn: 1.661 ± 0.24
4.352ThrPro: 4.352 ± 0.397
1.432ThrGln: 1.432 ± 0.196
3.092ThrArg: 3.092 ± 0.292
3.149ThrSer: 3.149 ± 0.328
2.92ThrThr: 2.92 ± 0.363
4.352ThrVal: 4.352 ± 0.433
1.718ThrTrp: 1.718 ± 0.258
2.033ThrTyr: 2.033 ± 0.27
0.0ThrXaa: 0.0 ± 0.0
Val
6.728ValAla: 6.728 ± 0.426
0.888ValCys: 0.888 ± 0.145
5.841ValAsp: 5.841 ± 0.465
5.154ValGlu: 5.154 ± 0.456
1.718ValPhe: 1.718 ± 0.191
5.411ValGly: 5.411 ± 0.499
1.403ValHis: 1.403 ± 0.19
3.235ValIle: 3.235 ± 0.286
2.749ValLys: 2.749 ± 0.257
4.581ValLeu: 4.581 ± 0.391
1.317ValMet: 1.317 ± 0.196
2.491ValAsn: 2.491 ± 0.246
3.579ValPro: 3.579 ± 0.311
2.548ValGln: 2.548 ± 0.251
4.438ValArg: 4.438 ± 0.462
4.237ValSer: 4.237 ± 0.378
4.438ValThr: 4.438 ± 0.391
5.211ValVal: 5.211 ± 0.409
1.059ValTrp: 1.059 ± 0.198
2.061ValTyr: 2.061 ± 0.265
0.0ValXaa: 0.0 ± 0.0
Trp
1.947TrpAla: 1.947 ± 0.215
0.515TrpCys: 0.515 ± 0.131
1.403TrpAsp: 1.403 ± 0.187
1.346TrpGlu: 1.346 ± 0.199
0.515TrpPhe: 0.515 ± 0.119
1.432TrpGly: 1.432 ± 0.187
0.687TrpHis: 0.687 ± 0.119
0.973TrpIle: 0.973 ± 0.157
0.744TrpLys: 0.744 ± 0.145
2.147TrpLeu: 2.147 ± 0.252
0.601TrpMet: 0.601 ± 0.165
0.802TrpAsn: 0.802 ± 0.157
1.117TrpPro: 1.117 ± 0.198
0.601TrpGln: 0.601 ± 0.132
1.746TrpArg: 1.746 ± 0.264
1.775TrpSer: 1.775 ± 0.235
1.346TrpThr: 1.346 ± 0.19
2.033TrpVal: 2.033 ± 0.245
0.802TrpTrp: 0.802 ± 0.149
0.601TrpTyr: 0.601 ± 0.162
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.149TyrAla: 3.149 ± 0.285
0.573TyrCys: 0.573 ± 0.126
2.119TyrAsp: 2.119 ± 0.235
2.262TyrGlu: 2.262 ± 0.225
0.659TyrPhe: 0.659 ± 0.125
3.178TyrGly: 3.178 ± 0.286
0.487TyrHis: 0.487 ± 0.133
1.031TyrIle: 1.031 ± 0.208
1.117TyrLys: 1.117 ± 0.218
2.691TyrLeu: 2.691 ± 0.275
0.401TyrMet: 0.401 ± 0.105
0.83TyrAsn: 0.83 ± 0.154
1.203TyrPro: 1.203 ± 0.198
1.117TyrGln: 1.117 ± 0.17
2.691TyrArg: 2.691 ± 0.262
1.346TyrSer: 1.346 ± 0.181
1.546TyrThr: 1.546 ± 0.229
2.176TyrVal: 2.176 ± 0.228
0.687TyrTrp: 0.687 ± 0.13
0.973TyrTyr: 0.973 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 239 proteins (34928 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski