Lutibacter sp. Hel_I_33_5

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Lutibacter; unclassified Lutibacter

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2634 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A559RYQ8|A0A559RYQ8_9FLAO Uncharacterized protein OS=Lutibacter sp. Hel_I_33_5 OX=1566289 GN=OD91_0488 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.52NNYY5 pKa = 9.87SKK7 pKa = 11.13FILKK11 pKa = 8.86TLLLFAILFGLEE23 pKa = 3.88TYY25 pKa = 10.01AQQNIIAQTQNITVQLDD42 pKa = 3.56ANGEE46 pKa = 4.25AIITPQQINNGSTATTGIQSLNLDD70 pKa = 3.62TSDD73 pKa = 5.29FNCTDD78 pKa = 2.94INGDD82 pKa = 3.69RR83 pKa = 11.84NFSYY87 pKa = 11.0LLDD90 pKa = 4.5GEE92 pKa = 4.49TDD94 pKa = 3.23ATGDD98 pKa = 4.04EE99 pKa = 4.34ILVDD103 pKa = 3.93DD104 pKa = 4.79TNLTTLTTQTIEE116 pKa = 3.44AWIRR120 pKa = 11.84PATGASDD127 pKa = 4.0NDD129 pKa = 3.31VLVAVRR135 pKa = 11.84NNIHH139 pKa = 6.24NSRR142 pKa = 11.84FSIHH146 pKa = 6.62INADD150 pKa = 3.29TNTIGIFNSTNWQTITTIINANQWYY175 pKa = 9.43HH176 pKa = 6.08IALVMNSTKK185 pKa = 10.36TLVYY189 pKa = 10.51LDD191 pKa = 3.92GAFEE195 pKa = 4.68GEE197 pKa = 3.9ISTGINTSLGANAPLSIGSPNDD219 pKa = 3.44TGWYY223 pKa = 7.61SEE225 pKa = 3.87KK226 pKa = 10.41WEE228 pKa = 4.36GNIDD232 pKa = 5.46DD233 pKa = 4.21LRR235 pKa = 11.84IWNDD239 pKa = 2.44EE240 pKa = 3.95RR241 pKa = 11.84TALEE245 pKa = 3.64IANNRR250 pKa = 11.84NIEE253 pKa = 4.11LVGNEE258 pKa = 4.22AGLVGYY264 pKa = 10.48FDD266 pKa = 5.05FNEE269 pKa = 4.58GTGNTITNLAGTVNGTANNSQNANWSTDD297 pKa = 3.14SPIEE301 pKa = 4.42SNGNTVNLTVTDD313 pKa = 3.86VNGNSATTPANVIVVDD329 pKa = 5.01NIAPIAPTLADD340 pKa = 3.43VTGEE344 pKa = 4.02CSATVTAPTTTDD356 pKa = 2.77NCAGTITGTTTDD368 pKa = 3.32SLTYY372 pKa = 7.42TTQGTHH378 pKa = 6.68IITWNFDD385 pKa = 3.58DD386 pKa = 5.63GNGNSTTASQNVIISDD402 pKa = 3.98STPPTAITNNISVNLDD418 pKa = 3.23ANGNASITPQQIDD431 pKa = 3.55NGSSDD436 pKa = 3.43ACGISSISCSRR447 pKa = 11.84VGGSGNGLTNFLNGTLNNGTADD469 pKa = 3.21IIEE472 pKa = 4.33YY473 pKa = 10.71SNSNAPNYY481 pKa = 7.41RR482 pKa = 11.84TSFGTTCNEE491 pKa = 3.02AWFARR496 pKa = 11.84GMLCDD501 pKa = 4.66LAPNQNDD508 pKa = 3.73PDD510 pKa = 3.71NSSRR514 pKa = 11.84QDD516 pKa = 3.41NNAGATWRR524 pKa = 11.84NTSPGNSYY532 pKa = 10.56GRR534 pKa = 11.84LVIDD538 pKa = 4.97LGSSQTINAMSIFQMFSDD556 pKa = 4.19GKK558 pKa = 6.81TTHH561 pKa = 6.72IEE563 pKa = 3.62AFAYY567 pKa = 10.21NGNLEE572 pKa = 4.36NPNSSSPGWNQLFPSTSVGAGVLNGTTVSSPLKK605 pKa = 8.72TTFQSTNTRR614 pKa = 11.84FIKK617 pKa = 10.58LYY619 pKa = 10.44AKK621 pKa = 10.52NDD623 pKa = 3.28GSLGFSGYY631 pKa = 9.92IEE633 pKa = 4.04LRR635 pKa = 11.84QVKK638 pKa = 8.98VFNTNEE644 pKa = 3.77SNACDD649 pKa = 4.49FNCDD653 pKa = 3.79DD654 pKa = 4.63LGEE657 pKa = 4.41NNVTLTVEE665 pKa = 4.49DD666 pKa = 3.87KK667 pKa = 11.19NGNISTKK674 pKa = 10.5DD675 pKa = 3.51AIVTIIDD682 pKa = 4.82AIAPALEE689 pKa = 4.32ATPADD694 pKa = 3.89LTVEE698 pKa = 4.25CHH700 pKa = 6.73SIPDD704 pKa = 3.56VATISATDD712 pKa = 3.38NCDD715 pKa = 3.33SNISVNFTEE724 pKa = 4.09QRR726 pKa = 11.84ADD728 pKa = 3.23GFSNDD733 pKa = 3.57NYY735 pKa = 10.28TLTRR739 pKa = 11.84TWSATDD745 pKa = 3.21QSGNTVSEE753 pKa = 4.39SQVITVQDD761 pKa = 3.69VTPPTIVGQNISVTLTASGTVSIIPQNVLNNGSDD795 pKa = 3.37TCSTVSYY802 pKa = 9.46TISQNTFGATDD813 pKa = 5.5AINSPVTIQLIGTDD827 pKa = 3.51EE828 pKa = 4.43SGNATTIPVQVTVIDD843 pKa = 4.05PVPVVITQNIVIQLDD858 pKa = 3.72DD859 pKa = 4.13NGTVVIDD866 pKa = 4.16PLQIDD871 pKa = 4.14NGSSSAVGIGSLSLDD886 pKa = 2.95ISAFNCEE893 pKa = 4.91DD894 pKa = 3.06INNPVTVTLTVTSTLGSSSTGTAIVTVQDD923 pKa = 4.35TIAPIAPILSDD934 pKa = 3.4ILGEE938 pKa = 4.32CSATATTPTTTDD950 pKa = 2.7NCAGSITGTTTDD962 pKa = 3.28SLEE965 pKa = 3.87YY966 pKa = 9.72TIQGTHH972 pKa = 6.87TITWSFDD979 pKa = 3.3DD980 pKa = 4.83GNGNTTTATQNVIVNDD996 pKa = 3.79TTAPIAITKK1005 pKa = 10.33NITIPLDD1012 pKa = 3.44ASGNAIITPQMINNGSSDD1030 pKa = 3.3NCEE1033 pKa = 3.68IDD1035 pKa = 5.75SITLDD1040 pKa = 3.29NTVFDD1045 pKa = 4.81CSNVDD1050 pKa = 4.98SSAQCNAGTALDD1062 pKa = 4.49LGADD1066 pKa = 3.46NDD1068 pKa = 4.04YY1069 pKa = 11.29VRR1071 pKa = 11.84IGDD1074 pKa = 3.91PFHH1077 pKa = 6.99EE1078 pKa = 5.16FSNQITVSWWVNFQGNSAWMTQSTFGMSSNNTNVWNMHH1116 pKa = 5.82ASGANGITWYY1126 pKa = 10.32INDD1129 pKa = 3.46NGAYY1133 pKa = 9.84RR1134 pKa = 11.84AVSLNTTAGWHH1145 pKa = 6.95LITGVADD1152 pKa = 3.7TTSTRR1157 pKa = 11.84LYY1159 pKa = 10.84LDD1161 pKa = 4.1GNLASTDD1168 pKa = 3.27TGINNIRR1175 pKa = 11.84VNANSEE1181 pKa = 3.74IAIGIDD1187 pKa = 3.17GRR1189 pKa = 11.84IPSRR1193 pKa = 11.84NRR1195 pKa = 11.84NFQTDD1200 pKa = 3.03EE1201 pKa = 3.72VLIYY1205 pKa = 10.87NRR1207 pKa = 11.84ALSEE1211 pKa = 4.27TEE1213 pKa = 3.88IQDD1216 pKa = 5.18LLMNCPDD1223 pKa = 3.4ISDD1226 pKa = 3.87NSLVGYY1232 pKa = 9.13WAMNEE1237 pKa = 4.05GSGNTIEE1244 pKa = 4.74DD1245 pKa = 3.53QSITGANGVLINMTDD1260 pKa = 3.79ADD1262 pKa = 4.18WVTSGINNIGAGSSNEE1278 pKa = 3.83VTLTVTDD1285 pKa = 3.74VNGNSSTAIANVTVNDD1301 pKa = 3.33ITAPTLNDD1309 pKa = 2.9VPANVTVEE1317 pKa = 4.06CDD1319 pKa = 4.2AIPNAATISASDD1331 pKa = 3.36NCGNPTITFVEE1342 pKa = 4.06EE1343 pKa = 4.47RR1344 pKa = 11.84IDD1346 pKa = 4.12GKK1348 pKa = 11.74SNDD1351 pKa = 3.61NYY1353 pKa = 10.36TLNRR1357 pKa = 11.84TWTATDD1363 pKa = 3.2QSEE1366 pKa = 4.21NSVSHH1371 pKa = 4.98TQIITVQDD1379 pKa = 3.28TTAPFFDD1386 pKa = 4.3ATPVDD1391 pKa = 3.37ITVEE1395 pKa = 4.23CEE1397 pKa = 4.11TVPAAATISASDD1409 pKa = 3.36NCGNPTITFVEE1420 pKa = 4.15EE1421 pKa = 4.48RR1422 pKa = 11.84IDD1424 pKa = 5.03GISNDD1429 pKa = 3.53NYY1431 pKa = 10.17TLTRR1435 pKa = 11.84TWTATDD1441 pKa = 3.92LSGNTVSEE1449 pKa = 4.28SQIITVQDD1457 pKa = 3.22VTAPTVVGQNISVTLTSTGTVSIVPEE1483 pKa = 3.92NVLDD1487 pKa = 4.39NGSDD1491 pKa = 3.26NCGTVTYY1498 pKa = 10.24SVSQDD1503 pKa = 2.96TFGATDD1509 pKa = 3.57AVNSPVTVQLTGTDD1523 pKa = 3.24ASGNATTVPVLVTVIDD1539 pKa = 4.01PVPVVITQNIVVQLDD1554 pKa = 3.65DD1555 pKa = 4.12NGTVVIDD1562 pKa = 4.31PSQIDD1567 pKa = 3.71NGSSSAVGIGSLSLDD1582 pKa = 2.95ISAFNCEE1589 pKa = 4.91DD1590 pKa = 3.06INNPVTVTLTVTSTLGSSSTGTAIVTVQDD1619 pKa = 4.35TIAPIAPILSDD1630 pKa = 3.4ILGEE1634 pKa = 4.32CSATATTPTTTDD1646 pKa = 2.7NCAGSITGTTTDD1658 pKa = 3.66YY1659 pKa = 11.67LEE1661 pKa = 4.18YY1662 pKa = 8.84TAQGIHH1668 pKa = 6.29VITWSFDD1675 pKa = 3.44DD1676 pKa = 4.34GNGNIATATQNVIVIDD1692 pKa = 3.78VTAPTAIAQDD1702 pKa = 3.25ISINLDD1708 pKa = 3.4DD1709 pKa = 5.21NGNATITPQQIDD1721 pKa = 3.68NDD1723 pKa = 4.42SNDD1726 pKa = 3.21ACGIASTSLDD1736 pKa = 3.95KK1737 pKa = 11.66NSFDD1741 pKa = 4.0CSNVGSSASSSSFSFDD1757 pKa = 3.76GIANANGDD1765 pKa = 4.38EE1766 pKa = 4.1ILVNDD1771 pKa = 4.38TSFSTLTAQTIEE1783 pKa = 3.66AWIRR1787 pKa = 11.84PASGSNGNEE1796 pKa = 3.24SWLAIRR1802 pKa = 11.84DD1803 pKa = 3.56RR1804 pKa = 11.84VGNSRR1809 pKa = 11.84YY1810 pKa = 10.11SIHH1813 pKa = 6.77TNPSANTIGVYY1824 pKa = 10.58NSTHH1828 pKa = 6.72GYY1830 pKa = 7.49RR1831 pKa = 11.84TISISTGINANQWYY1845 pKa = 8.62HH1846 pKa = 5.27VAMVMNTTRR1855 pKa = 11.84TLVYY1859 pKa = 11.04VNGTLAGQIAAGISTNHH1876 pKa = 6.56GANVPLSIGSPNDD1889 pKa = 3.02ASYY1892 pKa = 11.4YY1893 pKa = 8.15GEE1895 pKa = 4.09KK1896 pKa = 9.11WQGDD1900 pKa = 3.83MDD1902 pKa = 5.85DD1903 pKa = 3.8LRR1905 pKa = 11.84VWNTEE1910 pKa = 3.45RR1911 pKa = 11.84TATEE1915 pKa = 3.55IADD1918 pKa = 3.58NRR1920 pKa = 11.84NNEE1923 pKa = 3.97LTGNEE1928 pKa = 4.04VGLVGYY1934 pKa = 9.75FNFNEE1939 pKa = 4.43GSGNSVDD1946 pKa = 4.29NLAGTINGTANNSQTEE1962 pKa = 3.99NWIDD1966 pKa = 3.59EE1967 pKa = 4.48SPISGSTGNMVTLTVTDD1984 pKa = 3.69VNGNSSTATANVTVVDD2000 pKa = 4.61NISPEE2005 pKa = 3.82ISDD2008 pKa = 3.81PADD2011 pKa = 3.04INVFATSAAGAVVNYY2026 pKa = 6.75NTPIGTDD2033 pKa = 2.93NCTVTTALTAGLADD2047 pKa = 4.27GEE2049 pKa = 4.6TFPIGTTVVSYY2060 pKa = 8.75TATDD2064 pKa = 3.93GSGNTATATFNVIVTGLPPEE2084 pKa = 4.1IVVPEE2089 pKa = 5.1DD2090 pKa = 2.9ITVNNDD2096 pKa = 2.76SGEE2099 pKa = 4.17CGAIVNYY2106 pKa = 10.33AATEE2110 pKa = 4.26TVGIPASTITYY2121 pKa = 9.62DD2122 pKa = 3.37IEE2124 pKa = 4.8PGSFFAEE2131 pKa = 4.37GTVTVTATATNPVGTSTKK2149 pKa = 9.99TFTVTVNDD2157 pKa = 3.56NQLPSVLTQNLIINLDD2173 pKa = 3.66ANGNSTITPQQIDD2186 pKa = 3.48NGSSDD2191 pKa = 3.35NCGIASISLDD2201 pKa = 3.17NDD2203 pKa = 3.54TFNCSNVSTNTVTLTVTDD2221 pKa = 3.58VNGNSNSLTANVTVNDD2237 pKa = 3.37VTAPNVITQNIIIEE2251 pKa = 4.09LDD2253 pKa = 3.62EE2254 pKa = 4.78NGEE2257 pKa = 4.01ASITPADD2264 pKa = 3.7IDD2266 pKa = 4.01NNSTDD2271 pKa = 2.92ACGIEE2276 pKa = 4.97SYY2278 pKa = 11.34SLDD2281 pKa = 3.99KK2282 pKa = 11.44DD2283 pKa = 4.41SFDD2286 pKa = 5.21CSNTGDD2292 pKa = 3.63NTVVLTVTDD2301 pKa = 3.68VNGNDD2306 pKa = 3.45ASLSATVTVQDD2317 pKa = 4.41NIAPTVITQSYY2328 pKa = 10.47SIDD2331 pKa = 3.7LTNGVADD2338 pKa = 5.34IIPSNIDD2345 pKa = 2.9GGTFDD2350 pKa = 4.65NCDD2353 pKa = 3.19FTLSIDD2359 pKa = 3.87RR2360 pKa = 11.84DD2361 pKa = 3.81HH2362 pKa = 6.59FTCDD2366 pKa = 3.82DD2367 pKa = 3.63IGDD2370 pKa = 4.09HH2371 pKa = 6.21VVTLTATDD2379 pKa = 3.49ASGNSSSEE2387 pKa = 3.98TAIVSILGNVPTIGINDD2404 pKa = 3.57FNIVQTQKK2412 pKa = 11.22KK2413 pKa = 7.03NTIFLGFGPQSINLSTVVDD2432 pKa = 4.16GGSGFTYY2439 pKa = 10.38EE2440 pKa = 4.12WTTSTGEE2447 pKa = 4.27VVSNQANPTINPTVSTTYY2465 pKa = 10.86YY2466 pKa = 9.36VTVTNANGCTASTSIYY2482 pKa = 10.2VCVIDD2487 pKa = 4.8ARR2489 pKa = 11.84AYY2491 pKa = 9.95DD2492 pKa = 3.28RR2493 pKa = 11.84KK2494 pKa = 10.5GRR2496 pKa = 11.84YY2497 pKa = 7.95KK2498 pKa = 10.86GKK2500 pKa = 10.51VLVCHH2505 pKa = 5.99HH2506 pKa = 6.69TNGKK2510 pKa = 9.92KK2511 pKa = 8.63GTKK2514 pKa = 9.44HH2515 pKa = 5.3VMISISKK2522 pKa = 8.21NAVMQHH2528 pKa = 5.13LTKK2531 pKa = 10.67HH2532 pKa = 6.45GIGTDD2537 pKa = 3.36HH2538 pKa = 7.49ADD2540 pKa = 4.06SLGACNAQCVTNQTILARR2558 pKa = 11.84TKK2560 pKa = 9.87TINLSSVTTYY2570 pKa = 10.43PNPSNGIFEE2579 pKa = 4.38VRR2581 pKa = 11.84LSKK2584 pKa = 11.4AEE2586 pKa = 4.21DD2587 pKa = 3.33NTRR2590 pKa = 11.84LLLFDD2595 pKa = 4.2LTGKK2599 pKa = 10.77LILHH2603 pKa = 5.89KK2604 pKa = 9.7TISTKK2609 pKa = 9.9KK2610 pKa = 10.79ASMGSNNLPSGVYY2623 pKa = 8.69ILKK2626 pKa = 10.52VIYY2629 pKa = 10.2NDD2631 pKa = 3.99SISTKK2636 pKa = 10.53KK2637 pKa = 10.84LIIEE2641 pKa = 4.28KK2642 pKa = 10.67GKK2644 pKa = 10.69

Molecular weight:
277.62 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A559S2L1|A0A559S2L1_9FLAO NTE family protein OS=Lutibacter sp. Hel_I_33_5 OX=1566289 GN=OD91_1881 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.34KK6 pKa = 9.56RR7 pKa = 11.84KK8 pKa = 8.34RR9 pKa = 11.84AKK11 pKa = 10.25ISTHH15 pKa = 4.55KK16 pKa = 9.9RR17 pKa = 11.84KK18 pKa = 9.85KK19 pKa = 8.28RR20 pKa = 11.84ARR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.7KK27 pKa = 10.78KK28 pKa = 9.39KK29 pKa = 10.83

Molecular weight:
3.51 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2634

0

2634

952811

29

9603

361.7

40.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.861 ± 0.064

0.705 ± 0.017

5.787 ± 0.181

6.153 ± 0.08

5.327 ± 0.082

6.394 ± 0.097

1.607 ± 0.03

8.418 ± 0.084

8.647 ± 0.152

8.935 ± 0.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.872 ± 0.034

7.012 ± 0.068

3.128 ± 0.046

3.042 ± 0.035

3.002 ± 0.059

6.817 ± 0.054

6.335 ± 0.186

6.103 ± 0.049

0.962 ± 0.019

3.895 ± 0.042

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski